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Preferred walking speed (PWS) is lower when wearing a controlled ankle motion (CAM) boot, which can potentially make comparisons between footwear conditions difficult. Standardising walking speed accounts for this but lacks the ecological validity of PWS. The aim of this study was to compare acute biomechanical responses to CAM boot wear when walking is freely chosen and when it is controlled. Twelve healthy participants walked on an instrumented treadmill at their PWS and at three standardised speeds: 3, 4, and 5 km/h. They did so in three footwear conditions: (1) with a Rebound® Air Walker CAM boot on the right leg, (2) with a Rebound® Air Walker on the right leg and an Evenup Shoelift™ on the left, and (3) in normal footwear. Comparisons between footwear conditions were largely similar in the ipsilateral limb at PWS and at the standardised speeds, which included a decrease in total mechanical work and ankle joint work during CAM boot wear ( p < 0.001). At the standardised speeds, total mechanical work and hip joint work were lower during CAM boot wear than wearing normal shoes and the Evenup Shoelift™ ( p ≤ 0.014), although there were no differences between footwear conditions at PWS ( p ≥ 0.095). As such, acute responses to CAM boot wear are different when speed is standardised compared to when speed is freely chosen, meaning conclusions cannot necessarily be transferred between approaches. Based on these differences observed between walking speeds, it would be prudent for future studies to try to maintain ecological validity by using PWS.

Muscle-tendon unit (MTU) assessments can be categorised into local (e.g., tendon strain) or global (e.g., jump height) assessments. Although menstrual cycle phase may be a key consideration when implementing these assessments in female athletes, the reliability of many MTU assessments is not well defined within female populations. Therefore, the purpose of this study was to report the test-retest reliability of local and global MTU assessments during the early follicular phase of the menstrual cycle. Seventeen naturally menstruating females (age 28.5 ± 7.3 years) completed local and global MTU assessments during two testing sessions separated over 24–72 hours. Local tests included Achilles’ tendon mechanical testing and isometric strength of ankle plantar flexors and knee extensors, whereas global tests included countermovement, squat, and drop jumps, and the isometric midthigh pull. Based on intraclass correlation coefficient (ICC) statistics, poor to excellent reliability was found for local measures (ICC: 0.096–0.936). Good to excellent reliability was found for all global measures (ICC: 0.788–0.985), excluding the eccentric utilisation ratio (ICC 0.738) and most rate of force development metrics (ICC: 0.635–0.912). Isometric midthigh pull peak force displayed excellent reliability (ICC: 0.966), whereas force-time metrics ranged from moderate to excellent (ICC: 0.635–0.970). Excluding rate of force development (coefficient of variation [CV]: 10.6–35.9%), global measures (CV: 1.6–12.9%) were more reproducible than local measures (CV: 3.6–64.5%). However, local metrics directly measure specific properties of the MTU, and therefore provide valuable information despite lower reproducibility. The novel data reported here provides insight into the natural variability of MTU assessments within female athletes which can be used to enhance the interpretation of other female athlete data, especially that which aims to investigate other aspects of variability, such as the menstrual cycle.

This article discusses self-organization in cold atoms via light-mediated interactions induced by feedback from a single retro-reflecting mirror. Diffractive dephasing between the pump beam and the spontaneous sidebands selects the lattice period. Spontaneous breaking of the rotational and translational symmetry occur in the 2D plane transverse to the pump. We elucidate how diffractive ripples couple sites on the self-induced atomic lattice. The nonlinear phase shift of the atomic cloud imprinted onto the optical beam is the parameter determining coupling strength. The interaction can be tailored to operate either on external degrees of freedom leading to atomic crystallization for thermal atoms and supersolids for a quantum degenerate gas, or on internal degrees of freedom like populations of the excited state or Zeeman sublevels. Using the light polarization degrees of freedom on the Poincaré sphere (helicity and polarization direction), specific irreducible tensor components of the atomic Zeeman states can be coupled leading to spontaneous magnetic ordering of states of dipolar and quadrupolar nature. The requirements for critical interaction strength are compared for the different situations. Connections and extensions to longitudinally pumped cavities, counterpropagating beam schemes and the CARL instability are discussed.

We show that a Bose–Einstein Condensate illuminated by a far off-resonant optical pump field and its retroreflection from a feedback mirror can produce stable, localised structures known as optomechanical droplets. We show that these droplets could be used to measure the acceleration of a BEC via continuous monitoring of the position of the droplet via the optical intensity distribution.

We investigated the effect of probiotic Bacillus velezensis strains (LSSA01, 15AP4 and 2084) on pathogens causing post-weaning diarrhea in piglets (Enterotoxigenic Escherichia coli, Clostridium perfringens, Salmonella spp.). We studied the effect of B. velezensis and its cell-free supernatant on (1) pathogen growth; (2) IPEC-J2 cell cytokine and tight junction protein expression; (3) IPEC-J2 cell ‘wound’ recovery; (4) adhesion to IPEC-J2 cells and pathogen exclusion; and (5) Caenorhabditis elegans survival following pathogen exposure. Cell-free supernatant (CFS) from all strains inhibited the growth of ETEC F4 and F18 (by 36.9–53.2%; p < 0.05). One or more strains inhibited C. perfringens and Salmonella spp. (p < 0.05). Strain 2084 CFS increased IL-8 expression (+12.0% vs. control; p < 0.05; 6 h incubation), whereas LSSA01 CFS increased the expression of tight junction proteins (p < 0.05 vs. control; 6 h incubation) and accelerated 96 h ‘wound’ healing. Colony-forming units (CFUs) of all strains displayed a higher binding affinity to IPEC-J2 cells than 12 ETEC isolates, reduced adhesion of ETEC F4 and F18 and extended C. elegans survival over 30 d. The results indicate that probiotic B. velezensis strains have potential for use in the control of PWD pathogens.

Despite increasing interest in probiotics as antibiotic alternatives in swine production, few studies have directly compared the functional efficacy of different commercial probiotic formulations under controlled conditions. We conducted an in vitro study using porcine intestinal epithelial (IPEC-J2) and macrophage-like (3D4/21) cell models to compare the efficacy of three commercial probiotic consortia (C1: three strains of Bacillus velezensis; C2: B. licheniformis + B. subtilis; C3: Clostridium butyricum). Treatments were evaluated for their ability to inhibit pathogenic Escherichia coli, Clostridium perfringens, and Salmonella spp., enhance epithelial barrier integrity, and modulate immune responses. Experimental endpoints included pathogen inhibition assays, adhesion to IPEC-J2 cells, transepithelial electrical resistance (TEER), tight junction protein expression, and cytokine profiling via RT-qPCR and proteomics. Data were analyzed using the Kruskal–Wallis test with false discovery rate (FDR) control at 5%. C1 cell-free supernatant (CFS) strongly inhibited pathogen growth (84.8 ± 5.3% inhibition of ETEC F4+F18− vs. medium control; p < 0.05), whereas C2 had no effect, and C3 inhibited only one isolate. The coculture of IPEC-J2 cells with C1 CFS increased the expression of TJ proteins ZO-1, MUC13, and MUC20 (+12.9–46.6% vs. control; p < 0.001) and anti-inflammatory TGF-β; reduced pro-inflammatory IL-6 in LPS-stimulated 3D4/21 cells. In comparison, C2 and C3 showed minimal impact on epithelial barrier integrity and immune modulation, as indicated by negligible changes in TEER values, tight junction protein expression (ZO-1, MUC13, MUC20), and cytokine profiles relative to the control. In conclusion, C1 demonstrated greater in vitro efficacy than C2 (B. licheniformis + B. subtilis) and C3 (Clostridium butyricum), including pathogen inhibition assays, epithelial adhesion, TEER measurements, and cytokine modulation, suggesting its potential as a leading candidate for functional probiotic applications.

Preferred walking speed (PWS) is lower when wearing a controlled ankle motion (CAM) boot, which can potentially make comparisons between footwear conditions difficult. Standardising walking speed accounts for this but lacks the ecological validity of PWS. The aim of this study was to compare acute biomechanical responses to CAM boot wear when walking is freely chosen and when it is controlled. Twelve healthy participants walked on an instrumented treadmill at their PWS and at three standardised speeds: 3, 4, and 5 km/h. They did so in three footwear conditions: (1) with a Rebound® Air Walker CAM boot on the right leg, (2) with a Rebound® Air Walker on the right leg and an Evenup Shoelift[TM] on the left, and (3) in normal footwear. Comparisons between footwear conditions were largely similar in the ipsilateral limb at PWS and at the standardised speeds, which included a decrease in total mechanical work and ankle joint work during CAM boot wear (p < 0.001). At the standardised speeds, total mechanical work and hip joint work were lower during CAM boot wear than wearing normal shoes and the Evenup Shoelift[TM] (p [less than or equal to] 0.014), although there were no differences between footwear conditions at PWS (p [greater than or equal to] 0.095). As such, acute responses to CAM boot wear are different when speed is standardised compared to when speed is freely chosen, meaning conclusions cannot necessarily be transferred between approaches. Based on these differences observed between walking speeds, it would be prudent for future studies to try to maintain ecological validity by using PWS.

Racewalking is an unconventional form of competitive gait that elicits a unique loading profile on lower limb muscles and tendons. This study mapped the structural and mechanical properties of lower limbs in competitive racewalkers and made associations with World Athletics competition performance points. Fourteen international racewalkers (seven men, seven women) were recruited for this study. Static ultrasonography was used to quantify muscle and tendon morphological characteristics. An isokinetic dynamometer was used to measure passive musculotendinous stiffness of the triceps surae, isometric (seven knee/ankle angle combinations) and isokinetic (four angular velocities) strength parameters in the plantarflexors and dorsiflexors, and isokinetic knee flexion and extension strength at five angular velocities. Correlations were found between performance and fascicle length of gastrocnemius medialis (r = –0.569, p = 0.034), dorsiflexor strength at 120°/s (r = 0.649, p = 0.016) and knee flexor strength at 30°/s (r = 0.632, p = 0.020). No associations were found for isometric plantarflexion or passive stiffness properties. Overall, the study showed various morphological and mechanical properties are associated with performance in competitive racewalkers. These associations seem to be related to the specific and unique biomechanical characteristics of racewalking.

Hinged controlled ankle motion boots are used to incrementally increase ankle joint range of movement during rehabilitation following Achilles’ tendon rupture. This increased movement should induce mechanical stress on the tendon via cycles of stretching and shortening. However, research has yet to determine how this permitted range of movement influences tendon length change. Eight healthy individuals (age: 23 ± 2 y; stature = 1.70 ± 0.09 m; body mass = 67.7 ± 13.7 kg) walked at a self-selected speed on an instrumented, motorised treadmill in a hinged controlled ankle motion boot with three pre-established ankle ranges of movement: 0, 15, and 30°, which were all compared with walking in normal footwear. Kinematic and kinetic measurements were obtained using motion capture and the treadmill. Triceps surae mechanical characteristics, including Achilles’ tendon stretch, were obtained with B-mode ultrasonography. Achilles’ tendon stretch significantly (p < 0.001) increased as boot range of movement increased and was strongly correlated with measured ankle joint range of movement when the boot was set to a 15 or 30° range of movement (r ≥ 0.84, p ≤ 0.009). Increasing controlled ankle motion boot range of movement also increased ankle joint mechanical work done and total mechanical work done by the boot-wearing limb, which led to an increase in self-selected walking speed (all p < 0.001). These findings provide preliminary evidence that hinged controlled ankle motion boots have the capacity to provide a controlled mechanical stimulus to the Achilles’ tendon when range of movement is increased. This has possible clinical application for the early management of Achilles’ tendon rupture, potentially improving healing and functional outcomes if it can be translated into a patient population.

AbstractWe present an unusual movement disorder due to immune checkpoint inhibitors which responded rapidly to prednisolone.BackgroundImmune checkpoint inhibitors (ICIs) have revolutionised the scope of cancer therapy but can induce autoimmune effects on healthy organs, termed immune-related adverse events (irAEs). Their pathophysiology relies on the same mechanisms that confer anti-tumour activity and can affect any organ in the body. Neurological irAEs (n-irAEs) although rare, are potentially fatal. Differentiating n-irAEs from paraneoplastic syndromes and other neurological disorders can be challenging.CaseWe present a case of n-irAE in a patient with metastatic renal cell carcinoma treated with two cycles of ipilimumab/nivolumab. Following the second cycle, the patient developed subacute onset of postural and rest tremor, predominantly affecting her upper limbs, head and tongue, of variable amplitude, with bradykinesia of foot tapping. Her gait was broad-based. MRI brain was unremarkable. CSF was lymphocytic (WBC 103 cells, 90% lymphocytes) with elevated protein (1.30g/L), normal CSF:serum glucose ratio, negative CSF culture and viral PCR. She was treated for ICI-associated neurotoxicity and responded rapidly to high-dose steroids.ConclusionPhysicians should be aware of the diverse presentations of n-irAEs, including unusual movement disorders. Close collaboration between neurologists and oncologists is imperative in the management of these patients.