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Background The aim of this study was to determine the association between hypertriglyceridemia and hyperuricemia (HUA). Methods The study was conducted in 3884 subjects who had not received medication enrolled as a baseline. Each participant received at least three annual health check-ups between 2011 and 2017. The risk of hyperuricemia was assessed in four Quartiles (Q1 to Q4) according to TG levels using multivariate-adjusted logistic regression models. Results The total incidence rate of HUA was 62.3/1000 person-years. In the univariate analysis, the risk of hyperuricemia in people with hypertriglyceridemia was 2.353 times that of normal triglycerides, with a 95% confidence interval of (2.011, 2.754), and the risk of hyperuricemia in men was 1.86 times of female, and the 95% confidence interval is (1.634, 2.177). After adjusting the potential confounders, the relative risk RR of TG at Q2 Q3 Q4 was 1.445 (95%CI:1.114, 1.901), 2.075 (1.611, 2.674), 2.972 (2.322, 3.804). Conclusions TG is an independent risk factor for hyperuricemia. As the level of TG increases, the risk of HUA increases.

The seasonal freeze-thaw period is a critical time for restoring soil fertility. Winter irrigation during this period alters soil moisture in agricultural fields, which in turn affects the soil’s temperature, nutrients, and microbial factors within the soil micro-environment. Soil microorganisms play a crucial role in soil nitrogen fixation. Investigating the changes in soil microbial communities during the seasonal freeze-thaw period under winter irrigation conditions can help further understand the regulatory mechanisms of winter irrigation on the soil microenvironment. The experimental micro-areas were selected at the Key Laboratory of Modern Water-Saving Irrigation of Xinjiang Production &Construction Group. Based on soil moisture conditions, winter irrigation quotas of 0, 45, 90, and 135 mm were established. Soil samples were collected according to the seasonal freeze-thaw process in the experimental area, and measurements were taken for soil moisture, temperature, salinity, nutrients, as well as the quantity and diversity indices of soil bacteria and fungi. Using single-factor correlation and principal component analysis, the interactions between soil microbial indicators and their influencing factors under winter irrigation conditions were examined. Under winter irrigation conditions, during the seasonal freeze-thaw period, pH significantly affects the richness of soil bacterial populations and the quantity of bacteria ( P  < 0.05). Soil organic matter significantly influences the diversity of fungal populations ( P  < 0.05). The combined effects of soil moisture content, temperature, and pH significantly impact the quantity of soil microorganisms ( P  < 0.05), with soil moisture content being the determining factor. There is a significant correlation between the diversity and richness of soil microbial communities ( P  < 0.05). The results indicate that winter irrigation during the seasonal freeze-thaw period not only alters the composition and metabolic capacity of microbial communities but also affects the interrelationships among microbial diversity indices, thereby impacting microbial stability. Furthermore, a winter irrigation quota of 45 mm during the seasonal freeze-thaw period has multiple positive effects on the soil micro-environment, effectively promoting plant growth, improving soil health, and supporting the development of sustainable agriculture.

To investigate correlations between altered spontaneous brain activity, diabetic vascular disease, and cognitive function for patients with type 2 diabetes mellitus (T2DM) using resting-state functional magnetic resonance imaging (rs-fMRI). Rs-fMRI was performed for T2DM patients (n = 26) and age-, gender-, and education-matched non-diabetic control subjects (n = 26). Amplitude of low frequency fluctuations (ALFF) were computed from fMRI signals to measure spontaneous neuronal activity. Differences in the ALFF patterns between patients and controls, as well as their correlations with clinical variables, were evaluated. Compared with healthy controls, T2DM patients exhibited significantly decreased ALFF values mainly in the frontal and parietal lobes, the bilateral thalumi, the posterior lobe of the cerebellum, and increased ALFF values mainly in the visual cortices. Furthermore, lower ALFF values in the left subcallosal gyrus correlated with lower ankle-brachial index values (r = 0.481, p = 0.020), while lower ALFF values in the bilateral medial prefrontal gyri correlated with higher urinary albumin-creatinine ratio (r =  -0.418, p = 0.047). In addition, most of the regions with increased ALFF values in the visual cortices were found to negatively correlate with MoCA scores. These results confirm that ALFF are altered in many brain regions in T2DM patients, and this is associated with the presence of diabetic vascular disease and poor cognitive performance. These findings may provide additional insight into the neurophysiological mechanisms that mediate T2DM-related cognitive dysfunction, and may also serve as a reference for future research.

Recurrent spontaneous abortion (RSA) has a complex pathogenesis with an increasing prevalence and is one of the most intractable clinical challenges in the field of reproductive medicine. Quercetin (QCT) is an effective active ingredient extracted from Semen Cuscutae and Herba Taxilli used in traditional Chinese medicine for tonifyng the kidneys and promoting fetal restoration. Although QCT helps improve adverse pregnancy outcomes, the specific mechanism remains unclear. The trophoblast cell line HTR‐8/SVneo cultured in vitro was treated with different concentrations of QCT, and the cell counting kit‐8 assay, wound healing assay, transwell assay, and western blotting were used to evaluate the effects and mechanisms of QCT on the proliferation, migration, and invasion of HTR‐8/SVneo cells, respectively. To assess the expression levels of miR‐149‐3p and AKT serine/threonine kinase 1 (AKT1), quantitative real‐time polymerase chain reaction (qRT‐PCR) and western blotting analysis were performed. A dual‐luciferase reporter assay was used to investigate the potential regulatory relationship between miR‐149‐3p and AKT1. Our results showed that QCT promoted the proliferation, migration, and invasion of trophoblast cells, promoted the expression of MMP2, MMP9, and vimentin, and downregulated the expression of E‐cadherin. Mechanistically, QCT downregulated the expression of miR‐149‐3p and upregulated the expression of AKT1, and miR‐149‐3p directly targets AKT1, negatively regulating its expression. Overexpression of miR‐149‐3p and silencing of AKT1 counteracted the promotional effects of QCT on trophoblast proliferation, migration, and invasion. Taken together, QCT regulates the migration and invasion abilities of HTR‐8/SVneo cells through the miR‐149‐3p/AKT1 axis, which may provide a promising therapeutic approach for RSA.

Objective The prognostic value of tumor size in neuroblastoma (NB) patients has not been fully evaluated. Our purpose is to elucidate the prognostic significance of tumor size in surgery performed on neuroblastoma patients. Methods Neuroblastoma patients diagnosed from 2004 to 2015 were selected from the Surveillance, Epidemiology, and End Results Program (SEER) for the study. Univariate and multivariate Cox proportional hazard regression models were used to identify risk factors and the independent prognostic influences of tumor size on NB patients. Overall survival (OS) was analyzed through univariate Cox regression analysis. To determine the optimal cutoff value of tumor size, we first divided the cohort into three groups (≤5 cm, 5–10 cm, >10 cm). Subsequently, the patients were divided into two groups repeatedly, with tumor size at 1 cm intervals. The cutoff value that maximized prognostic outcome difference was selected. Furthermore, we performed the Kaplan–Meier methods to visually present differences in prognosis between the optimal tumor size cutoff value in different subgroups. Results A total of 591 NB patients who met the inclusion criteria were selected from the SEER database in this study. Cox analysis showed that age >1 year (HR = 2.42, p < 0.0001), originate from adrenal site (HR = 1.7, p = 0.014), distant stage (HR = 6.4, p < 0.0001), undifferentiated grade (HR = 1.94, p = 0.002), and large tumor size (HR = 1.5, p < 0.0001) independently predicted poor prognosis. For tumor size, there were significant differences in tumor size distribution in different ages, tumor grade, disease stage, and primary site subgroup but not in sex, race, and histology subgroup. Furthermore, both univariate (HR = 4.96, 95% CI 2.31–10.63, p < 0.0001) and multivariable analysis (HR = 2.8, 95% CI 1.29–6.08, p < 0.0001) indicated the optimal cutoff value of tumor size was 4 cm for overall survival of NB patients. Using a 4 cm of tumor size cutoff in subgroups, we found that it can identify poor prognosis patients whatever their age or primary site. Interestingly, tumor size of 4 cm cutoff can only identify unfavorable NB patients with diagnosis at distant‐stage disease, or differentiated grade tumor, but not with regional and local or undifferentiated tumor. Conclusions Tumor size is first to be recognized as a key prognostic factor of neuroblastoma patients and a cutoff value >4 cm might predict poor prognosis, which should be included in the evaluation of prognostic factors for NB. we found that tumor size could independently predict prognosis in NB patients, and a single cut‐off at 4 cm that maximized prognostic discrimination of NB children was essential for identify the poor prognosis subgroup.

In order to accurately grasp the characteristics and influencing factors of gas explosion in heading face, the mathematical model of gas explosion was determined. According to the actual size of a heading face of a coal mine, a 3D geometric model with a length of 100 m was established, and the effects of ignition energy and gas explosion equivalent on the gas explosion characteristics of the heading face were analyzed. The results show the following. (1) The mathematical models for numerical simulation of gas explosion can accurately simulate the gas explosion and its propagation process. The time-space step size has a great influence on the simulation results. The grid spacing for numerical simulation of mine gas explosion is determined to be 0.1 m and the time step length is determined to be 0.001 s. (2) The ignition energy has a limited effect on gas explosion characteristics. It only has a certain influence on the gas explosion process, but has little influence on the overpressure of shock wave. The larger the ignition energy is, the faster the explosion reaction speed is, and the maximum overpressure increases slightly. When the ignition energy increases to a certain value, the time of peak shock wave and the maximum overpressure both tend to be stable. The ignition energy has little effect on gas explosion characteristics when an explosion accident occurs underground with a large amount of gas accumulation. (3) The gas explosion equivalent has a great influence on the overpressure of gas explosion shock wave. The higher the explosion equivalent is, the greater the pressure is, and the peak value of the shock wave overpressure increases with the explosion equivalent as a power function. The research results have important guiding significance for the research and development of new technology for prevention and control of gas explosion.

Due to the eutectic reaction, the Mg 2 Si phase is inclined to creating an unfavorable fishbone shape at low solidification rates. This can significantly degrade the mechanical characteristics and become the main barrier to the practical application of Mg-Si based alloy. It is regarded that isothermal semi-solid treatment is an efficient technique to modulate the fishbone-shape eutectic Mg 2 Si phases in Mg-Si based alloys. In this work, a new type of Mg-11%Al-2%Zn-1%Si alloy is successfully fabricated through isothermal semi-solid treatment, and the influences of different process temperatures on microstructural evolution of Mg-11%Al-2%Zn-1%Si alloy are studied. The findings illustrate that the mean size of the α-Mg grains increases and their trendency of spheroidization becomes more evidently after held for 30 min at different process temperatures from 550 °C to 580 °C. Additionally, through isothermal semi-solid treatment, the eutectic Mg 2 Si phases in experimental alloy are totally changed from their original fishbone shape into polygon or granule shapes. With increasing the process temperatures, the mean size and morphologies of eutectic Mg 2 Si phases have no significantly changes. The morphological change theory of eutectic Mg 2 Si phases through isothermal semi-solid treatment is also studied.

Fluorescent probes with the binding selectivity to specific structures in DNAs or RNAs have gained much attention as useful tools for the study of nucleic acid functions. Here, chloro-substituted 2-amino-5,7-dimethyl-1,8-naphthyridine (ClNaph) was developed as a strong and highly selective binder for target orphan cytosine opposite an abasic (AP) site in the DNA duplexes. ClNaph was then conjugated with thiazole orange (TO) via an alkyl spacer (ClNaph–TO) to design a light-up probe for the detection of cytosine-related mutations in target DNA. In addition, we found the useful binding and fluorescence signaling of the ClNaph–TO conjugate to target C in AP site-containing DNA/RNA hybrid duplexes with a view toward sequence analysis of microRNAs.

MicroRNA-24 (miR-24) serves an important role in cell proliferation, migration and inflammation in various types of disease. In the present study, the biological function and molecular mechanism of miR-24 was investigated in association with the progression of age-associated cataracts. To the best of our knowledge the present study is the first to report that the expression of miR-24 was significantly increased in human anterior lens capsules affected by age-associated cataracts as well as lens epithelial cells (LECs) exposed to oxidative stress. Overexpression of miR-24 induced p53 expression and p53 was verified as a direct target of miR-24. Overexpression of miR-24 enhanced LEC death by directly targeting p53. The present study revealed that oxidative stress induced the upregulation of miR-24 and enhanced LEC death by directly targeting p53. These results suggest that the miR-24-p53 signaling pathway is involved in a novel mechanism of age-associated cataractogenesis and miR-24 may be a useful therapeutic target for age-associated cataracts.

Objective To explore the usefulness of highly sensitive nucleic acid detection for assisting with the accurate antiviral treatment of patients with cirrhosis that was caused by hepatitis. Methods There were 377 patients with hepatitis B with cirrhosis and 119 patients with hepatitis C with cirrhosis, either as hospitalized patients and outpatients, who were enrolled into the study. Among them, 299 were men and 197 were women between 23 and 82 years of age. All patients were examined using a domestic HBV DNA/HCV RNA test, which was negative in 162 cirrhosis with hepatitis B and 54 cirrhosis with hepatitis C patients (HBV DNA/HCV RNA <500 IU/mL). Prediction and analysis of the HBV DNA load using alanine aminotransferase (ALT) level was based on receiver operating characteristics (ROC) curve analysis. Results For patients with hepatitis C with cirrhosis, after the antiviral therapy, ALT, HCV RNA, and Child–Pugh grade were significantly improved compared with before treatment. ROC analysis results showed that an ALT level of 29 IU/mL was the most sensitive cutoff value to judge a positive HBV DNA load (sensitivity 1.0, specificity 0.237, Youden index 0.763). Conclusion Precise detection for patients with cirrhosis caused by hepatitis is required for accurate therapy.