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Objective From the perspective of informal social support, this paper analysed the impact of factors such as “Relationship with spouse”, “Relationship with Children”, “Financial support from children”, “Sibling support”, “Support from other friends and relatives” and “Borrowing costs” on the health poverty vulnerability of elderly people in rural China. Methods Based on the data of the China Health and Retirement Longitudinal Study (CHARLS) in 2018, the vulnerability of the rural elderly to health poverty was measured from two dimensions of health status and influencing factors of health status by the three-stage feasible generalized least square method. A quantile regression model was used to analyse the impact of six variables in the informal social support network on health poverty vulnerability: “Relationship with spouse”, “Relationship with children”, “Financial support from children”, \" Sibling support”, \" Support from other friends and relatives”, and “Borrowing costs”. Results When the poverty line standards were 2995 CNY/year and 4589 CNY/year, the health poverty vulnerability of the elderly population in rural China was 0.397 and 0.598 in 2018. In the analysis of informal social support, factors such as the relationship with spouse, relationship with children, borrowing costs, support from other friends and relatives, and sibling support had different impacts on the health poverty vulnerability of the rural elderly, who were classified into three groups according to their different vulnerabilities. Conclusion According to the analysis of the 2018 CHARLS database, the health poverty vulnerability of the elderly population was related to the informal social support network, and it is necessary to pay attention to the role of informal channels such as children, spouses, relatives and friends in daily care and financial support for rural elderly individuals. Meanwhile, the government and other formal organizations should also give full play to their supporting role for elderly individuals, who are highly vulnerable to health poverty, and their families.

Background Adverse childhood experiences are critical factors in depression and cognitive decrease, but the effect of adverse childhood health experiences (ACHEs) on cognitive function and the role of depression have not been fully studied. Methods Data were taken from the China Health and Retirement Longitudinal Study (CHARLS) of 2014 and 2018. This study used indicators of situational memory ability and mental status to measure cognitive capacity. Besides analyzing the different types of ACHEs, scores for ACHEs were calculated to represent the severity of ACHEs. The Center for Epidemiologic Studies Depression Scale (CES-D) was used to assess depression. The analysis of this study employed two different analytical strategies in order to examine the mediated effects of depression. We used Sobel’s test and Baron and Kenny’s causal step approach, which utilized a generalized least squares regression model. Furthermore, a logistic regression model was used to evaluate the robustness of the Karlson-Holm-Breen (KHB) approach. Results In this study, 6301 individuals who met the requirements of the study were included. We found that being confined to bed (ACHE3) (β=-0.3846, p = 0.022) in childhood had a negative impact on cognitive function. Similarly, ACHEs had a negative effect on cognitive function (β=-0.0819, p = 0.090). And after the depression had been introduced into the model, the regression coefficient of ACHEs on cognitive function was no longer significant (β=-0.0170, p = 0.727). The Sobel test showed that for ACHE3, the mediated proportion of the total effect of depression was 36.92%. While for ACHEs, the proportion of the mediated effect of depression was 70.11%. Finally, a robustness test of the mediating effect using the KHB method revealed that the mediating effect still existed. Further, based on different gender, age, and educational levels, the heterogeneity test indicated that the relationship between ACHEs and cognitive function and mediating effects of the depression were different as well as passing the robustness test of the interaction. Conclusion The decline in cognition had been shown to be correlated with ACHEs and depression mediated this relationship. Positive interventions might help to improve cognitive performance in individuals suffering from ACHEs and depression.

Background We aimed to explore the association between WeChat usage and depression in the Chinese middle-aged and elderly and the role of social participation. Methods Data were obtained from China Health and Retirement Longitudinal Study (CHARLS) of 2018. The dependent variable was depressive symptoms, measured with the 10-item Center for Epidemiologic Studies Depression Scale (CES-D-10). We used the propensity score matching (PSM) to match the WeChat users with the non-WeChat users. Correlations between WeChat usage and depressive symptoms were verified by using logistic regression and linear regression, and the mediating role of social participation was verified by using stepwise regression and KHB method. Results Four thousand five hundred forty-five samples were ultimately matched for analysis in this study. After including all control variables, results of logistic regression showed that WeChat usage was significantly associated with a lower prevalence of depression (aOR:0.701,95% CI: 0.605–0.812). And the results of linear regression showed that WeChat usage was associated with lower levels of depression which was significant ( p  < 0.001). The results of the stepwise regression and the KHB method showed a mediating role of social participation in WeChat usage and depressive symptoms. Among the four types of social participation, the mediating effect of recreational activities was significant, while the mediating effects of voluntary activities, cultural activities, and other activities were not significant. Meanwhile, the effect of WeChat usage on depression and the mediating effect of social participation were heterogeneous because of differences in age and gender. Conclusion Social participation partly mediated the effect between WeChat usage and depression in middle-aged and older adults. Among the four types of social participation, only recreational activities had a mediating effect. Encouraging more active social participation and other types of social activities should be considered to improve the mental health of the middle-aged and older adults in China through social media usage.

Introduction Several studies have proved that Polygenic Risk Score (PRS) is a potential candidate for realizing precision screening. The effectiveness of low-dose computed tomography (LDCT) screening for lung cancer has been proved to reduce lung cancer specific and overall mortality, but the cost-effectiveness of diverse screening strategies remained unclear. Methods The comparative cost-effectiveness analysis used a Markov state-transition model to assess the potential effect and costs of the screening strategies incorporating PRS or not. A hypothetical cohort of 300,000 heavy smokers entered the study at age 50–74 years and were followed up until death or age 79 years. The model was run with a cycle length of 1 year. All the transition probabilities were validated and the performance value of PRS was extracted from published literature. A societal perspective was adopted and cost parameters were derived from databases of local medical insurance bureau. Sensitivity analyses and scenario analyses were conducted. Results The strategy incorporating PRS was estimated to obtain an ICER of CNY 156,691.93 to CNY 221,741.84 per QALY gained compared with non-screening with the initial start age range across 50–74 years. The strategy that screened using LDCT alone from 70–74 years annually could obtain an ICER of CNY 80,880.85 per QALY gained, which was the most cost-effective strategy. The introduction of PRS as an extra eligible criteria was associated with making strategies cost-saving but also lose the capability of gaining more LYs compared with LDCT screening alone. Conclusion The PRS-based conjunctive screening strategy for lung cancer screening in China was not cost-effective using the willingness-to-pay threshold of 1 time Gross Domestic Product (GDP) per capita, and the optimal screening strategy for lung cancer still remains to be LDCT screening for now. Further optimization of the screening modality can be useful to consider adoption of PRS and prospective evaluation remains a research priority.

Introduction This study investigated the effects of falls on self-rated health and anxiety symptoms and the moderating role of psychological resilience in Chinese older persons with chronic multimorbidity. Methods Data were taken from the 2018 Chinese Longitudinal Healthy Longevity Survey (CLHLS). We used a linear regression model to evaluate the associations among falls and self-rated health and anxiety symptoms, the moderating role of psychological resilience was verified by moderation analysis, and we also used a replacement model to test robustness. Finally, the results of the study were further verified via heterogeneity analysis through subgroup regression. Results A total of 2933 people aged 60 years or older with chronic multimorbidity were included in our study. The linear regression results revealed that falls were significantly negatively correlated with the self-rated health symptoms of older Chinese people with chronic multimorbidity (β = -0.1703, p  < 0.01) and significantly positively correlated with anxiety symptoms (β = 0.5590, p  < 0.01). Among the moderating effects, we found that psychological resilience played a moderating role between falls and anxiety symptoms (β = − 0.151 [-0.217, -0.084], p  < 0.01). Finally, we found heterogeneity in the study results by sex, residence and number of chronic diseases. Conclusion Falls are associated with poorer self-rated health and higher anxiety levels among older persons with chronic multimorbidity in China. High levels of psychological resilience have a moderating effect on the development of anxiety symptoms.

Circulation of genotype VII Newcastle disease virus (NDV) has posed a great threat for the poultry industry worldwide. Antibodies against Hemagglutinin-neuraminidase (HN), a membrane protein of NDV with critical roles in NDV infection, have been reported to provide chickens protection from NDV infection. In this study, we comprehensively analyzed the in vivo antibody responses against the linear antigenic domains of the HN protein from genotype VII NDV using a yeast surface display system. The results revealed four distinct regions of HN, P1 (1-52aa), P2 (53-192aa), P3 (193-302aa) and P4 (303-571aa), respectively, according to their antigenic potency. Analysis by FACS and ELISA assay indicated P2 to be the dominant linear antigenic domain, with the immunogenic potency to protect the majority of chickens from NDV challenge. In contrast, the P1, P3 and P4 domains showed weak antigenicity in vivo and could not protect chickens from NDV challenge. These results provide important insight into the characteristic of humoral immune responses elicited by HN of NDV in vivo.

Background: This study aimed to investigate the potential mediation effect of adverse childhood school neighborhood friendship experiences (ACSNFEs) in the relationship between childhood parental emotions and depressive symptoms. Methods: The study extracted data from 9489 participants from the China Health and Retirement Longitudinal Study (CHARLS) of 2014 and 2020. Depressive symptoms were assessed by the 10‐item Center for Epidemiological Studies Depression Scale (CES‐D‐10). Stepwise regression based on least squares regression models, bootstrap tests, and Karlson–Holm–Breen (KHB)‐based logit regression models were applied to analyze. Results: Negative childhood parental emotions ( β = 0.2030 and p < 0.001), negative childhood mother’s emotions ( β = 0.3399 and p < 0.001), and negative childhood father’s emotions ( β = 0.3866 and p < 0.001) were all significantly associated with higher severity of depressive symptoms. Bootstrap tests showed that the proportion of ACSNFEs mediated for childhood parental emotions was 14.03%. For childhood mother’s emotions and childhood father’s emotions, the mediating proportions were 15.32% and 13.57%, respectively. Moreover, KHB tests showed that the mediating effect still existed. Conclusions: The association between childhood parental emotions and depressive symptoms was partly mediated by ACSNFEs. Focus on developing parental emotional management ability, actively guiding parents to help children develop high‐quality friendships, and promoting the development of psychological health.

The epithelial-mesenchymal transition (EMT) plays an important role in renal fibrosis. Defective fatty acid oxidation (FAO) is the metabolic hallmark in injured tubular epithelial cells (TECs) contributing to renal fibrogenesis. Dysregulated cytoskeleton is believed to be an important element of renal injury and its function has been linked to cell metabolism, but little is known about the influence of the cytoskeleton on FAO in TECs. Herein, we aimed to investigate the protective effects and molecular mechanisms of pharmacological stabilization of the cytoskeleton against unilateral ureteric obstruction (UUO) -induced renal tubulointerstitial fibrosis. TGF-β1-treated primary tubular epithelial cells (PTECs) and UUO mice served as renal interstitial fibrosis (RIF) models, with or without pharmacological cytoskeleton stabilization by Bis-T-23 (TargetMol, T30479; 20 mg/kg/day intraperitoneally in vivo; 5 μM in vitro). Biomarkers, HE staining, PAS staining, Masson staining, Sirius red staining, and TUNEL assay were used to assess kidney function and apoptosis. Real-time quantitative PCR (qPCR), Western blotting and immunohistochemistry (IHC) were performed to measure mRNA and protein expression levels respectively. PTECs viability was assessed by CCK-8 assay. Bulk mRNA sequencing was used for transcriptomic analysis of cellular metabolism. FITC-phalloidin fluorescence staining was used to detect the cytoskeleton. Fluorescent co-staining was used to detect the interactions between lipid droplets (LDs) and mitochondria. Pharmacological stabilization of the cytoskeleton alleviated tubulointerstitial injury, functional changes and TEC damage in UUO mouse models. Stabilization of cytoskeleton restored FAO and enhanced FAO-associated respiration impaired by UUO injury. Here, we demonstrate that cytoskeleton rearrangement is a critical regulator of mitochondrial FAO in TECs both in vitro and in vivo. We further show that this cytoskeleton-dependent regulation of FAO occurs through altered interactions between LDs and mitochondria. These findings indicate that cytoskeleton stabilization is required to maintain TECs metabolism and that therapeutic manipulation of cytoskeleton remodeling could protect the kidney from fibrogenesis conditions.

Circulation of genotype VII Newcastle disease virus (NDV) has posed a great threat for the poultry industry worldwide. Antibodies against Hemagglutinin-neuraminidase (HN), a membrane protein of NDV with critical roles in NDV infection, have been reported to provide chickens protection from NDV infection. In this study, we comprehensively analyzed the in vivo antibody responses against the linear antigenic domains of the HN protein from genotype VII NDV using a yeast surface display system. The results revealed four distinct regions of HN, P1 (1-52aa), P2 (53-192aa), P3 (193-302aa) and P4 (303-571aa), respectively, according to their antigenic potency. Analysis by FACS and ELISA assay indicated P2 to be the dominant linear antigenic domain, with the immunogenic potency to protect the majority of chickens from NDV challenge. In contrast, the P1, P3 and P4 domains showed weak antigenicity in vivo and could not protect chickens from NDV challenge. These results provide important insight into the characteristic of humoral immune responses elicited by HN of NDV in vivo.