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[...]the lung autonomic nervous system may be provoked by pulmonary exposure, which could then result in autonomic nervous system imbalance [15]; the levels of stress hormones may also be altered [16]. Ninety-five percent of the total Chinese population was covered by social health insurance schemes by the end of 2017 [21]. [...]hospital admission records can provide reliable and timely information on the health status of a geographically defined population in China. Since January 1, 2013, class 3 hospitals in China have been mandated to automatically submit inpatient discharge records to the HQMS on a daily basis, in a nationally standardized format. All data used were anonymized and de-identified prior to analysis, under the supervision of Bureau of Medical Administration, National Health Commission of the People’s Republic of China. Because the data were analyzed at the aggregate level with no individual identifiers involved, institutional review board approval and participant written consent were not required for this study.

The mitogen-activated protein kinase (MAPK) cascade is a highly conserved signaling transduction module that transduces extracellular stimuli into intracellular responses in plants. Early studies of plant MAPKs focused on their functions in model plants. Based on the results of whole-genome sequencing, many MAPKs have been identified in horticultural plants, such as tomato and apple. Recent studies revealed that the MAPK cascade also plays crucial roles in the biotic and abiotic stress responses of horticultural plants. In this review, we summarize the composition and classification of MAPK cascades in horticultural plants and recent research on this cascade in responses to abiotic stresses (such as drought, extreme temperature and high salinity) and biotic stresses (such as pathogen infection). In addition, we discuss the most advanced research themes related to plant MAPK cascades, thus facilitating research on MAPK cascade functions in horticultural plants.

Recognition of histone-modified nucleosomes by specific reader domains underlies the regulation of chromatin-associated processes. Whereas structural studies revealed how reader domains bind modified histone peptides, it is unclear how reader domains interact with modified nucleosomes. Here, we report the cryo-electron microscopy structure of the PWWP reader domain of human transcriptional coactivator LEDGF in complex with an H3K36-methylated nucleosome at 3.2–Å resolution. The structure reveals multivalent binding of the reader domain to the methylated histone tail and to both gyres of nucleosomal DNA, explaining the known cooperative interactions. The observed cross-gyre binding may contribute to nucleosome integrity during transcription. The structure also explains how human PWWP domain-containing proteins are recruited to H3K36-methylated regions of the genome for transcription, histone acetylation and methylation, and for DNA methylation and repair.The cryo-EM structure of the PWWP reader domain of the transcriptional coactivator LEDGF in complex with an H3K36-methylated nucleosome reveals multivalent binding of the reader domain to the methylated histone tail and to both gyres of nucleosomal DNA.

University physical education is an important public basic course in colleges and universities. The traditional teaching is usually within the class time specified in the training program; the teacher teaches the students the basic physical education fundamentals so that the students can master the basic skills of sports, thus improving the students’ sports level and physical quality. An improved genetic algorithm is proposed to reduce the problem of slow convergence and partial convergence of the fundamental genetic algorithm for intelligent grouping systems. To ensure the group’s stability and variety, the algorithm can rapidly extend the search space by repeatedly rejecting similar individuals. Therefore, this study proposes a new method of intelligent grouping based on the improved genetic algorithm. The new method can overcome the problem of premature convergence of the algorithm more efficiently and easily than the traditional algorithm. A large number of experiments have proved that the proposed algorithm meets all the requirements of physical education very well. The algorithm can automatically generate test papers with moderate difficulty and reasonable structure.

The rapid emergence of drug resistant Staphylococcus aureus ( S. aureus ) poses a serious threat to public health globally. Silver (Ag)-based antimicrobials are promising to combat antibiotic resistant S. aureus , yet their molecular targets are largely elusive. Herein, we separate and identify 38 authentic Ag + -binding proteins in S. aureus at the whole-cell scale. We then capture the molecular snapshot on the dynamic action of Ag + against S. aureus and further validate that Ag + could inhibit a key target 6-phosphogluconate dehydrogenase through binding to catalytic His185 by X-ray crystallography. Significantly, the multi-target mode of action of Ag + (and nanosilver) endows its sustainable antimicrobial efficacy, leading to enhanced efficacy of conventional antibiotics and resensitization of MRSA to antibiotics. Our study resolves the long-standing question of the molecular targets of silver in S. aureus and offers insights into the sustainable bacterial susceptibility of silver, providing a potential approach for combating antimicrobial resistance. Silver (Ag) has been used as an antimicrobial agent since a long time, but its molecular mechanism of action was not elucidated due to technical challenges. Here, the authors develop a mass spectrometric approach to identify the Ag-proteome in Staphylococcus aureus , and capture a molecular snapshot of the dynamic bactericidal mode of action of Ag through targeting multiple biological pathways.

The TATA box-binding protein (TBP) is highly conserved throughout eukaryotes and plays a central role in the assembly of the transcription preinitiation complex (PIC) at gene promoters. TBP binds and bends DNA, and directs adjacent binding of the transcription factors TFIIA and TFIIB for PIC assembly. Here, we show that yeast TBP can bind to a nucleosome containing the Widom-601 sequence and that TBP–nucleosome binding is stabilized by TFIIA. We determine three cryo-electron microscopy (cryo-EM) structures of TBP–nucleosome complexes, two of them containing also TFIIA. TBP can bind to superhelical location (SHL) –6, which contains a TATA-like sequence, but also to SHL +2, which is GC-rich. Whereas binding to SHL –6 can occur in the absence of TFIIA, binding to SHL +2 is only observed in the presence of TFIIA and goes along with detachment of upstream terminal DNA from the histone octamer. TBP–nucleosome complexes are sterically incompatible with PIC assembly, explaining why a promoter nucleosome generally impairs transcription and must be moved before initiation can occur.

In order to investigate the effects of strain rate and water saturation on the energy dissipation and crack growth of tuff, uniaxial compression tests were carried out on dry and water saturated tuff with different strain rates using an electro-hydraulic servo press and a 50 mm diameter split Hopkinson pressure rod (SHPB) device. High-speed camera and Image J image analysis software were used to obtain the crack growth process of the specimen under impact load, and fractal dimension was introduced to quantitatively study the crack growth degree. The results show that more than 90% of the energy is stored in the specimen as elastic energy when it reaches the peak stress under static load. The average total energy of water-saturated specimens is 67.55% of that of dry specimens. The average energy dissipation density of water-saturated specimens under 0.3 MPa, 0.4 MPa and 0.5 MPa air pressure is 0.79, 0.91 and 0.92 times of that of dry specimens, respectively. Water-saturated specimens will deteriorate and thus reduce their energy storage and energy absorption effects. The reflected energy, transmitted energy, absorbed energy and incident energy are linear, logarithmic and linear functions, respectively, and the energy absorptivity and specific energy absorptivity of water-saturated specimens are lower than those of dry specimens. Due to the existence of “stefan” effect, the increase of energy dissipation density of water-saturated specimen at high strain rate is greater than that of dry specimen. The mean fractal dimension of water-saturated specimens under 0.3 MPa, 0.4 MPa and 0.5 MPa is 1.09, 1.05 and 1.16 times that of dry specimens. At the same strain rate, the number and width of cracks in water-saturated specimens are larger than that in dry specimens. Water-saturated behavior reduces the energy absorption capacity of tuff, increases the fractal dimension of crack growth, and significantly reduces the resistance of water-saturated rock to external loads.

Magnéli phase titanium oxides, also called titanium sub-oxides (TinO2n−1, 4 < n < 9), are a series of electrically conducting ceramic materials. The synthesis and applications of these materials have recently attracted tremendous attention because of their applications in a number of existing and emerging areas. Titanium sub-oxides are generally synthesized through the reduction of titanium dioxide using hydrogen, carbon, metals or metal hydrides as reduction agents. More recently, the synthesis of nanostructured titanium sub-oxides has been making progress through optimizing thermal reduction processes or using new titanium-containing precursors. Titanium sub-oxides have attractive properties such as electrical conductivity, corrosion resistance and optical properties. Titanium sub-oxides have played important roles in a number of areas such as conducting materials, fuel cells and organic degradation. Titanium sub-oxides also show promising applications in batteries, solar energy, coatings and electronic and optoelectronic devices. Titanium sub-oxides are expected to become more important materials in the future. In this review, the recent progress in the synthesis methods and applications of titanium sub-oxides in the existing and emerging areas are reviewed.

The Qilian Mountain ecosystems play an irreplaceable role in maintaining ecological security in western China. Vegetation, as an important part of the ecosystem, has undergone considerable changes in recent decades in this area, but few studies have focused on the process of vegetation change. A long normalized difference vegetation index (NDVI) time series dataset based on remote sensing is an effective tool to investigate large-scale vegetation change dynamics. The MODerate resolution Imaging Spectroradiometer (MODIS) NDVI dataset has provided very detailed regional to global information on the state of vegetation since 2000. The aim of this study was to explore the spatial-temporal characteristics of abrupt vegetation changes and detect their potential drivers in the Qilian Mountain area using MODIS NDVI data with 1 km resolution from 2000 to 2017. The Breaks for Additive Season and Trend (BFAST) algorithm was adopted to detect vegetation breakpoint change times and magnitudes from satellite observations. Our results indicated that approximately 80.1% of vegetation areas experienced at least one abrupt change from 2000 to 2017, and most of these areas were distributed in the southern and northern parts of the study area, especially the area surrounding Qinghai Lake. The abrupt browning changes were much more widespread than the abrupt greening changes for most years of the study period. Environmental factors and anthropogenic activities mainly drove the abrupt vegetation changes. Long-term overgrazing is likely the main cause of the abrupt browning changes. In addition, our results indicate that national ecological protection policies have achieved positive effects in the study area.

Global emergence of Gram-negative bacteria carrying the plasmid-borne resistance genes, bla MBL and mcr , raises a significant challenge to the treatment of life-threatening infections by the antibiotics, carbapenem and colistin (COL). Here, we identify an antirheumatic drug, auranofin (AUR) as a dual inhibitor of metallo-β-lactamases (MBLs) and mobilized colistin resistance (MCRs), two resistance enzymes that have distinct structures and substrates. We demonstrate that AUR irreversibly abrogates both enzyme activity via the displacement of Zn(II) cofactors from their active sites. We further show that AUR synergizes with antibiotics on killing a broad spectrum of carbapenem and/or COL resistant bacterial strains, and slows down the development of β-lactam and COL resistance. Combination of AUR and COL rescues all mice infected by Escherichia coli co-expressing MCR-1 and New Delhi metallo-β-lactamase 5 (NDM-5). Our findings provide potential therapeutic strategy to combine AUR with antibiotics for combating superbugs co-producing MBLs and MCRs. Multi-drug resistant pathogens remain a serious public health threat. Here, Sun and colleagues identify a role for auranofin, which is normally used as a drug for rheumatoid arthritis, for reversing antibiotic resistance to carbapenem and colistin.