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Ferroptosis is an iron-dependent programmed cell death characterized by reactive oxygen species-induced lipid peroxidation and resultant membrane damage. Recent research has elucidated the mechanism of ferroptosis and investigated the relationship between ferroptosis and various diseases, including degenerative diseases, cancer, and inflammation. Ferroptosis is associated with inflammation-related intestinal diseases such as colitis and colitis-associated cancer. New insights into the role of ferroptosis in the pathogenesis of inflammation-related gut diseases have suggested novel therapeutic targets. In this review, we summarize current information on the molecular mechanisms of ferroptosis and describe its emerging role and therapeutic potential in inflammation-related intestinal diseases.

Background and Aims To explore the inhibition mechanism of Saccharomyces boulardii ( S. boulardii ) on ulcerative colitis (UC) carcinogenesis. Methods C57BL/6 mice were treated with azoxymethane and dextran sulfate sodium (AOM/DSS) to develop a UC carcinogenesis model. The treatment group was lavaged with S. boulardii (5 × 10 7  CFU/d) for 12 weeks. The mice were sacrificed and the tumor load in the treatment group was compared with that of a control group. The levels of TNF-α and IL-6 in colon tissue were measured by enzyme-linked immunosorbent assays. The influence of S. boulardii on TNF-α and IL-6 regulation was also investigated using different colon cell lines. Differences in intestinal microbiota in both stool and intestinal mucosa samples were assessed using 16S rDNA sequencing. Results S. boulardii treatment reduced AOM/DSS-induced UC carcinogenesis in mice, as indicated by the reduced tumor load and reduced TNF-α and IL-6 levels in vivo, as well its effects on TNF-α and IL-6 activities in vitro. Significant changes in both fecal and mucosal microbiota were observed among the control, the AOM/DSS treated, and AOM/DSS plus S. boulardii treated groups. For fecal microbiota, the AOM/DSS treated group was lower in Lactobacillus , but higher in Oscillibacter and Lachnoclostridium than the control group. After intervention with S. boulardii , the percentage of Bacillus and Lactococcus increased, but Lachnoclostridium , Oscillibacter, Bacteroides, and Pseudomonas decreased. For the intestinal mucosal microbiota, the AOM/DSS treated group was lower in Bifidobacterium and Ruminococcaceae_UCG-014 and higher in Alloprevotella than the control group. After S. boulardii exposure, the percentage contributions of Lachnoclostridium and Lachnospiraceae_NK4A136 increased. Conclusions S. boulardii effectively reduced UC carcinogenesis in an AOM/DSS induced mice model. This positive result can likely be attributed to the reduction of TNF-α and IL-6 levels or the blockade of their function combined with alterations to the intestinal microbiota.

Based on panel data from 173 Chinese listed companies in the new energy vehicle industry from 2016 to 2023, this study constructs a two-way fixed effects model to examine the impact of data elements on corporate innovation capability. Systematically address issues and validate results through multiple measurement approaches, including robustness checks, instrumental variables methods, moderation effect analysis, and heterogeneity tests. The results indicate: (1) Data elements significantly enhance the innovation capability of new energy vehicle enterprises, and a conclusion that remains robust after a series of endogeneity and robustness checks. (2) Moderating effect tests reveal that human resources strengthen the relationship between data elements and corporate innovation capability. (3) Heterogeneity analysis shows that, in terms of capital sources, data elements have a more substantial promoting effect on the innovation capability of domestic enterprises compared to foreign-funded ones; regionally, the innovation-driven effect of data elements is more pronounced in eastern and central China than in the western region. This study not only offers practical guidance for new energy vehicle enterprises to allocate data elements and human resources effectively, but also provides an empirical basis for policymakers to formulate market-oriented data policies, thereby offering a new perspective for enhancing the innovation capabilities of new energy vehicle enterprises.

Alkylnitriles play important roles in many fields because of their unique electronic properties and structural characteristics. Incorporating cyanoalkyl with characteristic spectroscopy and reactivity properties into amino acids and peptides is of special interest for potential imaging and therapeutic purposes. Here, we report a copper-catalyzed asymmetric cyanoalkylation of C(sp 3 )-H. In the reactions, glycine derivatives can effectively couple with various cycloalkanone oxime esters with high enantioselectivities, and the reaction can be applied to the late-stage modification of peptides with good yields and excellent stereoselectivities, which is useful for modern peptide synthesis and drug discovery. The mechanistic studies show that the in situ formed copper complex by the coordination of glycine derivatives and chiral phosphine Cu catalyst can not only mediate the single electronic reduction of cycloalkanone oxime ester but also control the stereoselectivity of the cyanoalkylation reaction. Incorporating cyanoalkyl moieties into amino acids and peptides is of interest for potential imaging and therapeutic purposes. Here, the authors developed a Cu-catalyzed asymmetric cyanoalkylation of glycine derivatives for the synthesis of α-cyanoalkylate amino acids and peptide modification.

Background Participating in regular physical activity contributes to significant improvements of quality of life (QOL) in adults. Understanding psychosocial factors that influence physical activity and QOL in working adults may have important implications for future interventions aimed at improving their health. The major purpose of this study was to investigate the psychosocial predictors of physical activity and QOL among Shanghai working adults. Methods Participants were 238 working adults ( M age  = 51.6 ± 5.6) living in Shanghai communities, China. They completed previously validated questionnaires assessing their perceptions of stress, social support from friends, self-efficacy, physical activity, and QOL. Pearson correlations were computed to assess the associations among physical activity, QOL, and psychosocial variables. Path analysis was used to test the predictive strengths of psychosocial factors on physical activity and QOL among Shanghai working adults. Results The results indicated that stress had directly negative relationships on self-efficacy and QOL. Social support had directly positive relationships on self-efficacy, physical activity, and QOL. Physical activity had directly positive relationship on QOL. Self-efficacy and physical activity mediated the influences of stress and social support on QOL. Conclusions: Stress and social support from friends were two important sources of self-efficacy, all of which facilitated more physical activity participation. Lower stress, higher social support, and more physical activity may directly increase QOL among Shanghai working adults. The mediating roles of self-efficacy and physical activity should be taken into account in managing stress and social support in order to promote QOL among Shanghai working adults.

Background Approximately 20% to 30% of preterm infants may develop white matter injury. Early detection of brain injury is of great significance for clinical practice. The snake model is a boundary detection technology used in image processing and computer vision. It automatically locates the closed boundaries of objects in the image by simulating the behavior of elastic snakes. The quantitative ultrasound segmentation technique using the snake model can improve the accuracy of the diagnosis of early white matter injury. Methods In this retrospective single-center study, cranial ultrasound scans from 60 preterm infants with clinically confirmed WMD and 40 healthy controls were analyzed. Five regions of interest (ROIs) around key brain structures were automatically delineated by the snake model, and mean grayscale values were measured. Reproducibility was assessed via inter- and intra-observer analyses, and receiver operating characteristic (ROC) curves determined optimal thresholds for WMD prediction. Results The snake model achieved a mean area under the ROC curve (AUC) of 0.87 for ROI 1 , 0.85 for ROI 2 , and 0.82 for ROI 3 , with corresponding sensitivities of 0.82, 0.70, and 0.63 and specificities of 0.88, 0.85, and 0.88. The average Dice coefficient across ROIs was 0.75, and The total coefficient of variation was 6.7%, which was less than 10%, indicating high reproducibility. Conclusions Quantitative analysis of ultrasound images with the snake model demonstrates promising accuracy and reproducibility for early WMD detection in preterm infants. Future multicenter studies with larger cohorts are warranted to validate these findings. Clinical trial number Not applicable.

Vitamin D deficiency and insufficiency pose global public health challenges, yet research on serum vitamin D levels in the 0–17-year-old age group in southeastern China remains limited. This study aimed to fill this gap by investigating serum 25(OH)D levels in children in the region aged 0–17 years, contributing crucial data for understanding vitamin D nutritional status. Liquid chromatography‒mass spectrometry/mass spectrometry (LC‒MS/MS) technology was used. Vitamin D testing was integrated into routine diagnostic procedures for 11,116 children in Wujiang District, Suzhou City. Among the 0–17-year age group, comprising 6348 boys and 4768 girls, the prevalence of serum 25(OH)D deficiency and insufficiency was 21.4% and 31.0%, respectively. The median serum 25(OH)D concentration was 29.72 ng/mL (21.84–39.84 ng/mL) in boys compared to 28.48 ng/mL (20.65–39.23 ng/mL) in girls. Seasonal variations were observed, with median serum 25(OH)D concentrations of 29.02 ng/mL (20.73–39.72 ng/mL) in spring, 28.79 ng/mL (21.53–39.37 ng/mL) in summer, 30.12 ng/mL (22.00–39.70 ng/mL) in autumn, and 28.58 ng/mL (19.97–39.46 ng/mL) in winter. Statistically significant differences were noted in the serum 25(OH)D levels during autumn and winter. In conclusion, the rate of adequate vitamin D levels in local children was 47.5%, revealing a relatively high prevalence of vitamin D deficiency (21.4%) and insufficiency (31.0%), especially during the post-preschool period. Advocating for vitamin D supplementation in children is crucial for ensuring adequate vitamin D support.

Systemic autoinflammatory diseases are caused by mutations in genes that function in innate immunity. Here, we report an autoinflammatory disease caused by loss-of-function mutations in OTULIN (FAM105B), encoding a deubiquitinase with linear linkage specificity. We identified two missense and one frameshift mutations in one Pakistani and two Turkish families with four affected patients. Patients presented with neonatal-onset fever, neutrophilic dermatitis/panniculitis, and failure to thrive, but without obvious primary immunodeficiency. HEK293 cells transfected with mutated OTULIN had decreased enzyme activity relative to cells transfected with WT OTULIN, and showed a substantial defect in the linear deubiquitination of target molecules. Stimulated patients’ fibroblasts and peripheral blood mononuclear cells showed evidence for increased signaling in the canonical NF-κB pathway and accumulated linear ubiquitin aggregates. Levels of proinflammatory cytokines were significantly increased in the supernatants of stimulated primary cells and serum samples. This discovery adds to the emerging spectrum of human diseases caused by defects in the ubiquitin pathway and suggests a role for targeted cytokine therapies.

Background Central nervous system (CNS) tumors are the most common solid tumors in children and the leading cause of cancer-related death in the latter. Currently, the incidence rate exceeds that of leukemia and ranks first in the incidence of malignant tumors in children. Methods The epidemiological data on childhood CNS tumors were collected from the Chinese Cancer Registry Annual Report. The annual percent change (APC) of incidence and mortality-rate changes were estimated via Joinpoint regression. Due to a lack of pertinent data, we performed a system review on the clinical-pathological characteristics in Chinese publications. Results There was no significant increase in the incidence rate (APC: -0.1, 95% CI: -1.5 to 1.3), but there was a significant increase in the mortality rate (APC: 1.8, 95% CI: 0.3 to 3.4) for childhood CNS tumors. In the subgroup analysis, there were significant increases in both the incidence and mortality rates in rural areas (APC in the incidence: 6.2, 95% CI: 2.4 to 10.2; APC in mortality: 4.4, 95% CI: 0.4 to 8.4). The most common location and type of childhood CNS were, respectively, the cerebral hemisphere (25.5%, 95% CI: 21.7% to 29.4%) and astrocytomas (26.8%, 95% CI: 23.9% to 29.6%). Conclusions The epidemiological trends, and the relevant prediction, highlighted the need to pay continual attention to childhood CNS tumors, and the clinicopathology evinced its own distinctive characteristics. Timely detection and effective treatment must be further promoted regarding childhood CNS tumors with a view to decreasing the disease burden, especially in rural areas.

A large amount of rabbit manure is produced with the development of the rabbit industry, which will cause environmental pollution without proper treatment. Rabbit manure compost may be suitable for seedling cultivation, considering its low moisture, low heavy metal, high lignocellulose, and good fertilizer effect. In this study, a pre-proportioning test of growing media was conducted to optimize the ratio of perlite and vermiculite with peat/rabbit manure compost according to their physicochemical properties. Then, based on the results of the first proportioning optimization, the mixing ratio of rabbit manure compost and peat was further optimized using a bioassay. In this bioassay, salt-tolerant calendula ( Calendula officinalis L.) and salt-intolerant cucumber ( Cucumis sativus L.) were selected as test plants. The seedling effects (e.g., seedling emergence percentage, plant growth parameters, plant biomass, and nutrient effects) were evaluated. It was shown in the results that the rabbit manure compound growing media could be used for the seedlings, and suitable seedling performance was obtained with the increase of the total porosity (5.0%–61.2%), organic matter content (8.3%–39.9%), and nutrient elements from the rabbit manure compost. From the perspective of seedling emergence, there was no significant difference between rabbit manure compound media and peat treatment, in which the highest emergence percentages were >90%. At the same time, the nutrient performance of plant aboveground was significantly increased in rabbit manure compound growing media compared to peat treatment. In particular, the contents of P and Mg were increased by 31%–141.4% and 80.4%–107.8% for calendula and by 82.6%–117.4% and 35.1%–67.6% for cucumber, respectively. It was indicated in the two-step optimization that the rabbit manure compost proportion of 30%–50% (that is, 60%–100% instead of peat) was more suitable. Additionally, the greenhouse gas emission could be reduced by using rabbit manure compost replacing peat, and the greenhouse gas emission reduction potential would be 3.65 × 10 5 –4.06 × 10 8  kg CO 2 -equivalent/year in China, which has important ecological significance.