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Single-cell transcriptome profiling of an adult human cell atlas of 15 major organs
Background
As core units of organ tissues, cells of various types play their harmonious rhythms to maintain the homeostasis of the human body. It is essential to identify the characteristics of cells in human organs and their regulatory networks for understanding the biological mechanisms related to health and disease. However, a systematic and comprehensive single-cell transcriptional profile across multiple organs of a normal human adult is missing.
Results
We perform single-cell transcriptomes of 84,363 cells derived from 15 tissue organs of one adult donor and generate an adult human cell atlas. The adult human cell atlas depicts 252 subtypes of cells, including major cell types such as T, B, myeloid, epithelial, and stromal cells, as well as novel
COCH
+
fibroblasts and FibSmo cells, each of which is distinguished by multiple marker genes and transcriptional profiles. These collectively contribute to the heterogeneity of major human organs. Moreover, T cell and B cell receptor repertoire comparisons and trajectory analyses reveal direct clonal sharing of T and B cells with various developmental states among different tissues. Furthermore, novel cell markers, transcription factors, and ligand-receptor pairs are identified with potential functional regulations in maintaining the homeostasis of human cells among tissues.
Conclusions
The adult human cell atlas reveals the inter- and intra-organ heterogeneity of cell characteristics and provides a useful resource in uncovering key events during the development of human diseases in the context of the heterogeneity of cells and organs.
Journal Article
Hai ma xian sheng
After Mrs. Seahorse lays her eggs on Mr. Seahorse's belly, he drifts through the water, greeting other fish fathers who are taking care of their eggs.
BOOK
Engineering Auger recombination in colloidal quantum dots via dielectric screening
Auger recombination is the main non-radiative decay pathway for multi-carrier states of colloidal quantum dots, which affects performance of most of their optical and optoelectronic applications. Outstanding single-exciton properties of CdSe/CdS core/shell quantum dots enable us to simultaneously study the two basic types of Auger recombination channels—negative trion and positive trion channels. Though Auger rates of positive trion are regarded to be much faster than that of negative trion for II-VI quantum dots in literature, our experiments find the two rates can be inverted for certain core/shell geometries. This is confirmed by theoretical calculations as a result of geometry-dependent dielectric screening. By varying the core/shell geometry, both types of Auger rates can be independently tuned for ~ 1 order of magnitude. Experimental and theoretical findings shed new light on designing quantum dots with necessary Auger recombination characteristics for high-power light-emitting-diodes, lasers, single-molecular tracking, super-resolution microscope, and advanced quantum light sources.
Designing core/shell quantum dots with desired optoelectronic properties remains a challenge. Here, the authors investigate the negative and positive trions dynamics within a quantum dot, proposing an Auger engineering strategy based on geometry-dependent dielectrics for tuning optical properties.
Journal Article
5G networks : fundamental requirements, enabling technologies, and operations management
A reliable and focused treatment of the emergent technology of fifth generation (5G) networks This book provides an understanding of the most recent developments in 5G, from both theoretical and industrial perspectives. It identifies and discusses technical challenges and recent results related to improving capacity and spectral efficiency on the radio interface side, and operations management on the core network side. It covers both existing network technologies and those currently in development in three major areas of 5G: spectrum extension, spatial spectrum utilization, and core network and network topology management. It explores new spectrum opportunities; the capability of radio access technology; and the operation of network infrastructure and heterogeneous Qoe provisioning. 5G Networks: Fundamental Requirements, Enabling Technologies, and Operations Management is split into five sections: Physical Layer for 5G Radio Interface Technologies; Radio Access Technology for 5G Networks; 5G Network Interworking and Core Network Advancements; Vertical 5G Applications; and R&D and 5G Standardization. It starts by introducing emerging technologies in 5G software, hardware, and management aspects before moving on to cover waveform design for 5G and beyond; code design for multi-user MIMO; network slicing for 5G networks; machine type communication in the 5G era; provisioning unlicensed LAA interface for smart grid applications; moving toward all-IT 5G end-to-end infrastructure; and more. This valuable resource: -Provides a comprehensive reference for all layers of 5G networks -Focuses on fundamental issues in an easy language that is understandable by a wide audience -Includes both beginner and advanced examples at the end of each section -Features sections on major open research challenges 5G Networks: Fundamental Requirements, Enabling Technologies, and Operations Management is an excellent book for graduate students, academic researchers, and industry professionals, involved in 5G technology.
Book
Suspension electrolyte with modified Li+ solvation environment for lithium metal batteries
Designing a stable solid–electrolyte interphase on a Li anode is imperative to developing reliable Li metal batteries. Herein, we report a suspension electrolyte design that modifies the Li
+
solvation environment in liquid electrolytes and creates inorganic-rich solid–electrolyte interphases on Li. Li
2
O nanoparticles suspended in liquid electrolytes were investigated as a proof of concept. Through theoretical and empirical analyses of Li
2
O suspension electrolytes, the roles played by Li
2
O in the liquid electrolyte and solid–electrolyte interphases of the Li anode are elucidated. Also, the suspension electrolyte design is applied in conventional and state-of-the-art high-performance electrolytes to demonstrate its applicability. Based on electrochemical analyses, improved Coulombic efficiency (up to ~99.7%), reduced Li nucleation overpotential, stabilized Li interphases and prolonged cycle life of anode-free cells (~70 cycles at 80% of initial capacity) were achieved with the suspension electrolytes. We expect this design principle and our findings to be expanded into developing electrolytes and solid–electrolyte interphases for Li metal batteries.
Stable solid–electrolyte interphases on Li anodes are crucial for reliable Li metal batteries. A suspension electrolyte design that modifies the Li
+
solvation environment in liquid electrolytes and creates inorganic-rich interphases on Li is now reported.
Journal Article
Interplay between plasmon and single-particle excitations in a metal nanocluster
Plasmon-generated hot carriers are used in photovoltaic or photochemical applications. However, the interplays between the plasmon and single-particle excitations in nanosystems have not been theoretically addressed using
ab initio
methods. Here we show such interplays in a Ag
55
nanocluster using real-time time-dependent density functional theory simulations. We find that the disappearance of the zero-frequency peak in the Fourier transform of the band-to-band transition coefficient is a hallmark of the plasmon. We show the importance of the
d
-states for hot-carrier generations. If the single-particle
d
-to-
s
excitations are resonant to the plasmon frequency, the majority of the plasmon energy will be converted into hot carriers, and the overall hot-carrier generation is enhanced by the plasmon; if such resonance does not exist, we observe an intriguing Rabi oscillation between the plasmon and hot carriers. Phonons play a minor role in plasmonic dynamics in such small systems. This study provides guidance on improving plasmonic applications.
Plasmons can enhance hot-carrier generation for efficient photochemical reactions, but the interplay between plasmons and single-particle excitations are difficult to capture in models. Here, the authors use real-time time-dependent density functional theory to study these interactions in silver nanocrystals.
Journal Article
Dictionary of environmental and climate change law
This dictionary defines terms employed in international agreements, national legislation and scholarly legal studies related to comparative and international environmental law and the emerging law of climate change. Each term also includes its pinyin translation in order to facilitate accessing the Mandarin variants of each term.
Book
Facet development during platinum nanocube growth
An understanding of how facets of a nanocrystal develop is critical for controlling nanocrystal shape and designing novel functional materials. However, the atomic pathways of nanocrystal facet development are mostly unknown because of the lack of direct observation. We report the imaging of platinum nanocube growth in a liquid cell using transmission electron microscopy with high spatial and temporal resolution. The growth rates of all low index facets are similar until the {100} facets stop growth. The continuous growth of the rest facets leads to a nanocube. Our calculation shows that the much lower ligand mobility on the {100} facets is responsible for the arresting of {100} growing facets. These findings shed light on nanocrystal shape-control mechanisms and future design of nanomaterials.
Journal Article