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Previous studies highlighting the pivotal function of the S100A8 protein have shown that inflammation and vascular endothelial harm play a major role in deep vein thrombosis (DVT) development, as evidenced by earlier studies highlighting the pivotal function of the S100 calcium-binding protein A8 (S100A8). Therefore, we aimed to establish a connection between S100A8 and DVT and investigate the role of S100A8 in DVT development. Blood specimens were taken from 23 patients with DVT and 31 controls. The fluctuation and association for S100A8 and interleukin-1 beta (IL-1β) in the specimens was assessed using enzyme-linked immunosorbent assay. We also used the human recombinant protein S100A8 to activate human umbilical vein endothelial cells and created a rat model to explore the possible relationship between them. Studies have shown that the infiltration of S100A8 sustains local inflammation and thrombus formation, which may exacerbate DVT by amplifying NLRP3/Caspase-1/IL-1β signals in the vascular endothelial cells.

Herein, we aimed to identify blood biomarkers that compensate for the poor specificity of D-dimer in the diagnosis of deep vein thrombosis (DVT). S100A8 was identified by conducting protein microarray analysis of blood samples from patients with and without DVT. We used ELISA to detect S100A8, VCAM-1, and ICAM-1 expression levels in human blood and evaluated their correlations. Additionally, we employed human recombinant protein S100A8 to induce human umbilical vein endothelial cells and examined the role of the TLR4/MAPK/VCAM-1 and ICAM-1 signaling axes in the pathogenic mechanism of S100A8. Simultaneously, we constructed a rat model of thrombosis induced by inferior vena cava stenosis and detected levels of S100A8, VCAM-1, and ICAM-1 in the blood of DVT rats using ELISA. The associations of thrombus tissue, neutrophils, and CD68-positive cells with S100A8 and p38MAPK, TLR4, and VCAM-1 expression levels in vein walls were explored. The results revealed that blood S100A8 was significantly upregulated during the acute phase of DVT and activated p38MAPK expression by combining with TLR4 to enhance the expression and secretion of VCAM-1 and ICAM-1, thereby affecting the occurrence and development of DVT. Therefore, S100A8 could be a potential biomarker for early diagnosis and screening of DVT.

Deep vein thrombosis (DVT) is a significant contributor to the global disease burden. This study aimed to identify metabolic biomarkers to compensate for the limitation in specificity and singular nature of D-dimer test. UHPLC–MS was used to examine γ-butylbetaine and l -carnitine levels in clinical and rat blood and urine at different stages of DVT along with analysis of their diagnostic titer and correlation analyses with DVT. γ-Butylbetaine and l -carnitine levels in plasma and urine which trends were similar in plasma and urine of humans and rats with DVT were lower in patients with DVT and rats than those in the control group ( P  < 0.05); in human plasma, area under the curve (AUC) of γ-butylbetaine and l -carnitine combined with D-dimer was 0.914 ( P  < 0.001) in the acute group and 0.895 ( P  < 0.001) in the subacute group, respectively. In human urine, AUC of l -carnitine combined with γ-butylbetaine in the subacute group was 0.855 ( P  < 0.001). γ-Butylbetaine and l -carnitine can be used to screen and diagnose DVT at different phases. Moreover, γ-butylbetaine and l -carnitine expression levels are similar in humans and rats, providing precise indicators and animal models for in-depth study of the mechanisms underlying DVT development.

Background Expanding the number of biomarkers is imperative for studying the etiology and improving venous thromboembolism prediction. In this study, we aimed to identify promising biomarkers or targeted therapies to improve the detection accuracy of early-stage deep vein thrombosis (DVT) or reduce complications. Methods Quantibody Human Cytokine Antibody Array 440 (QAH-CAA-440) was used to screen novel serum-based biomarkers for DVT/non-lower extremity DVT (NDVT). Differentially expressed proteins in DVT were analyzed using bioinformatics methods and validated using a customized array. Diagnostic accuracy was calculated using receiver operating characteristics, and machine learning was applied to establish a biomarker model for evaluating the identified targets. Twelve targets were selected for validation. Results Cytokine profiling was conducted using a QAH-CAA-440 (RayBiotech, USA) quantimeter array. Cross-tabulation analysis with Venn diagrams identified common differential factors, leading to the selection of 12 cytokines for validation based on their clinical significance. These 12 biomarkers were consistent with the results of previous array analysis: FGF-6 (AUC = 0.956), Galectin-3 (AUC = 0.942), EDA-A2 (AUC = 0.933), CHI3L1 (AUC = 0.911), IL-1 F9 (AUC = 0.898), Dkk-4 (AUC = 0.88), IG-H3 (AUC = 0.876), IGFBP (AUC = 0.858), Gas-1 (AUC = 0.858), Layilin (AUC = 0.849), ULBP-2 (AUC = 0.813)and FGF-9 (AUC = 0.773). These cytokines are expected to serve as biomarkers, targets, or therapeutic targets to differentiate DVT from NDVT. Conclusions EDA-A2, FGF-6, Dkk-4, IL-1 F9, Galentin-3, Layilin, Big-h3, CHI3L1, ULBP-2, Gas-1, IGFBP-5, and FGF-9 are promising targets for DVT diagnosis and treatment.

Aiming at the higher requirements on the performance of composite materials put forward by new specialized equipment, the development of new high-performance winding resin is carried out. First, basing on the epoxy curing system, the selection of the main resin has been completed, we select the appropriate curing agent according to the characteristics of the screened trifunctional glycidylamine epoxy resin and trifunctional alicyclic epoxy resin, the ratio is calculated and optimised, the curing system is established, and the design of the resin formulation system is completed. Second, according to the nature of the resin curing system, the toughening method is screened, and the synergistic enhancement of heat resistance and toughness of the resin is accomplished through the core-shell nano-toughening method. Finally, the glass transition temperature of the new high-performance winding resin T1 is 165.12°C, and the fracture toughness of the resin K IC can reach 1.74MPa-m 1/2 , and the annular tensile strength of the T1 resin-based T700 carbon fibre composites can reach 3500MPa, which meets the requirements of the design indexes of specialized equipment.

Objective: In this study, we evaluated the efficacy of tranexamic acid (TXA) and acute normovolemic hemodilution (ANH) with 6% hydroxyethyl starch (130/0.4) in minimizing blood loss during open partial liver resection. Coagulation function was assessed using thromboelastography (TEG) and hemostasis tests, while renal function changes were tracked through serum creatinine values post-surgery. Methods: Thirty patients undergoing open partial liver resection were allocated to two groups: Group T received TXA + ANH, and Group A received ANH alone. Blood was drawn from the radial artery under general anesthesia. Both groups received peripheral vein injections of 6% hydroxyethyl starch 130/0.4. Group T additionally received intravenous TXA. Primary outcomes included blood loss and allogeneic blood transfusions. TEG assessed coagulation status and renal function was monitored. Results: Group T demonstrated superior outcomes compared to Group A. Group T had significantly lower intraoperative blood loss (700 mL vs 1200 mL) and a lower bleeding rate per kilogram of body weight (13.3 mL/kg vs 20.4 mL/kg). Coagulation parameters favored Group T, with higher TEG maximum amplitude (55.91 mm vs 45.88 mm) and lower activated partial thromboplastin time (38.04 seconds vs 41.49 seconds). Neither group experienced acute renal injury or kidney function deficiency during hospitalization. Conclusion: TXA and ANH in a small dose during liver resection stabilize clotting, reduce blood loss by 6% compared to hydroxyethyl starch 130/0.4, and do not affect renal function. Keywords: acute normovolemic hemodilution, coagulation function, liver resection, thromboelastography, tranexamic acid

When the relation between the interconnected grid and the main system is weak, once a serious failure occurs in the tie line both the local small grid and the large grid, the local weakly connected grid will disconnect from the main grid and into island operation. Due to the small number of island operating units and the overall capacity and inertia are very small, the system frequency and voltage are easy to fluctuate in a large range, and operational stability is very prominent. This paper studied the effects the stability of isolated island grid for gusty wind and gradual wind. At the same time, the island system stable operation for different ratio of wind generator is also studied. Finally, by analyzing the regulating effect of reactive energy compensation device, voltage stability of wind electricity is improved.

From tensile overload to shot peening, there have been many attempts to extend the fatigue properties of metals. A key challenge with the cold work processes is that it is hard to avoid generation of harmful effects (e.g., the increase of surface roughness caused by shot peening). Pre-stress has a positive effect on improving the fatigue property of metals, and it is expected to strength Al-alloy without introducing adverse factors. Four pre-stresses ranged from 120 to 183 MPa were incorporated in four cracked extended-compact tension specimens by application of different load based on the measured stress–strain curve. Fatigue crack growth behavior and fractured characteristic of the pre-stressed specimens were investigated systematically and were compared with those of an as-received specimen. The results show that the pre-stress ranged from 120 to 183 MPa significantly improved the fatigue resistance of Al-alloy by comparison with that of the as-received specimen. With increasing pre-stress, the fatigue life first increases, then decrease, and the specimen with pre-stress of 158 MPa has the longest fatigue life. For the manner of pre-stress, no adverse factor was observed for increasing fatigue property, and the induced pre-stress reduced gradually till to disappear during subsequent fatigue cycling.

Photocatalytic two-electron oxygen reduction to produce high-value hydrogen peroxide (H 2 O 2 ) is gaining popularity as a promising avenue of research. However, structural evolution mechanisms of catalytically active sites in the entire photosynthetic H 2 O 2 system remains unclear and seriously hinders the development of highly-active and stable H 2 O 2 photocatalysts. Herein, we report a high-loading Ni single-atom photocatalyst for efficient H 2 O 2 synthesis in pure water, achieving an apparent quantum yield of 10.9% at 420 nm and a solar-to-chemical conversion efficiency of 0.82%. Importantly, using in situ synchrotron X-ray absorption spectroscopy and Raman spectroscopy we directly observe that initial Ni-N 3 sites dynamically transform into high-valent O 1 -Ni-N 2 sites after O 2 adsorption and further evolve to form a key *OOH intermediate before finally forming HOO-Ni-N 2 . Theoretical calculations and experiments further reveal that the evolution of the active sites structure reduces the formation energy barrier of *OOH and suppresses the O=O bond dissociation, leading to improved H 2 O 2 production activity and selectivity. Here, the authors explore how Ni single-atom sites on carbon nitride evolve under photocatalytic conditions. They show that this evolution plays a pivotal role in enhancing photocatalytic H 2 O 2 production.

The coupled green energy and chemical production by photocatalysis represents a promising sustainable pathway, which poses great challenges for the multifunction integration of catalytic systems. Here we show a promising green photocatalyst design using Cu-ZnIn 2 S 4 nanosheets and carbon dots as building units, which enables the integration of reaction, mass transfer, and separation functions in the nano-space, mimicking a nanoreactor. This function integration results in great activity promotion for benzyl alcohol oxidation coupled H 2 production, with H 2 /benzaldehyde production rates of 45.95/46.47 mmol g −1  h −1 , 36.87 and 36.73 times to pure ZnIn 2 S 4 , respectively, owning to the enhanced charge accumulation and mass transfer according to in-situ spectroscopies and computational simulations of the built-in electrical field. Near-unity selectivity of benzaldehyde is achieved via the effective separation enabled by the Cu(II)-mediated conformation flipping of the intermediates and subsequent π-π conjugation. This work demonstrates an inspiring proof-of-concept nanoreactor design of photocatalysts for coupled sustainable systems. Cu-doped ZnIn 2 S 4 nanosheets decorated with carbon dots are reported for the photocatalytic oxidation of organic alcohols coupled with H 2 evolution with enhanced yields due to efficient charge accumulation and mass transfer at the catalyst surface.