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The precise regulation of bone homeostasis and the balance between bone resorption and formation in periodontitis remain unclear. This study explores the role of long intergenic noncoding RNA-erythroid prosurvival (lincRNA-EPS) in inflammatory osteoclastogenesis and bone resorption. LincRNA-EPS knockout (KO) worsened LPS-induced alveolar bone resorption in vivo and osteoclast differentiation in vitro. Transcriptomics and protein sequencing showed dysregulated osteoclastogenesis and iron homeostasis without lincRNA-EPS, marked by increased expression of Lcn2 . Knockdown of Lcn2 in osteoclast precursors (OCPs) resulted in a reduction in the level of iron metabolism and osteoclastogenesis; however, the regulatory response was delayed in KO cells. Correspondingly, overexpression of lincRNA-EPS accelerated the regulation of iron metabolism. Further, reducing Lcn2 levels in wildtype mice alleviated periodontitis-related bone loss, but not in KO mice. Taken together, we identified the critical role of lincRNA-EPS in regulating osteoclastogenesis under inflammatory environment, by preventing excessive iron metabolism caused by Lcn2.

The fast and reliable protection is one key technology of flexible HVDC transmission system. The traditional flexible DC line protection is mainly based on single-ended fault characteristics, whose reliability is greatly affected by DC fault. In this paper, a single-ended protection of flexible HVDC transmission line based on line-mode power is proposed. The frequency-domain characteristics of line-mode power traveling wave under internal and external fault are analyzed theoretically. The line-mode power is converted by S-transform, the amplitude differences between the low and high frequencies are analyzed, and the amplitude ratio of low frequency to high frequency is constructed for the protection criterion. The simulation results on Zhangbei four-terminal ± 500 kV flexible HVDC transmission system by PSCAD illustrate the effectiveness of proposed algorithm under different fault types, fault locations and transition resistances, which can withstand the transition resistance 1000 Ω and 20 dB noise.

The large yellow croaker ( Larimichthys crocea ) is a cornerstone of China’s mariculture. This study integrates transcriptomic, proteomic, and metabolomic analyses to determine hypoxia adaptation mechanisms in an anti-flowing F1 generation (FDTL) compared with non-selected (FDCL) counterparts under 24-h hypoxic stress (2.0 mg/L dissolved oxygen). FDTL exhibited higher survival (67% vs. 42%). Transcriptomic analysis identified 852 differentially expressed genes, with significant enriched pathways including hypoxia-inducible factor signaling, glycolysis/gluconeogenesis, and IL-17-mediated immunity. Metabolomic profiling revealed 463 differential metabolites, predominantly associated with glycerophospholipid metabolism, arachidonic acid metabolism, and VEGF signaling. Proteomic screening detected 388 differentially abundant proteins, uniquely enriched in the cytokine-cytokine receptor interaction pathway. Cross-omics integration uncovered 37 shared pathways, with VEGF, GnRH, and C-type lectin receptor signaling pathways being co-regulated at the transcriptomic-metabolomic level. Notably, Core glycolysis-related genes and hypoxia-inducible factor-associated genes were markedly downregulated. This study confirmed that the anti-flowing strain exhibits a lower oxygen threshold for metabolic reprogramming, enabling sustained aerobic metabolic homeostasis under reduced oxygen levels. The integration of immune regulation and angiogenesis establishes a multi-layered hypoxia resistance network, providing molecular targets for breeding stress-tolerant fish. These findings highlight the FDTL’s superior adaptability to high-density offshore aquaculture and validate the effectiveness of targeted breeding strategies.

Nobiletin (NOB), a polymethoxy flavonoid, possesses anti-cancer and anti-inflammatory activities, has been reported that it played role in anti-osteoporosis treatment. However, previous research did not focus on practical use due to lack of hydrophilicity and cytotoxicity at high concentrations. The aim of this study was to develop a therapeutic formulation for osteoporosis based on the utilization of NOB. In this study, NOB-loaded poly(ethylene glycol)-block-poly(e-caprolactone) (NOB-PEG-PCL) was prepared by dialysis method. The effects on osteoclasts and anti-osteoporosis functions were investigated in a RANKL-induced cell model and ovariectomized (OVX) mice. Dynamic light scattering and transmission electron microscopy examination results revealed that the NOB-PEG-PCL had a round shape, with a mean diameter around 124 nm. The encapsulation efficiency and drug loading were 76.34±3.25% and 7.60±0.48%, respectively. The in vitro release of NOB from NOB-PEG-PCL showed a remarkably sustained releasing characteristic and could be retained at least 48 hrs in pH 7.4 PBS. Anti-osteoclasts effects demonstrated that the NOB-PEG-PCL significantly inhibited the formation of tartrate-resistant acid phosphatase (TRAP)-positive multinuclear cells stimulated by RANKL. Furthermore, the NOB-PEG-PCL did not produce cytotoxicity on bone marrow-derived macrophages (BMMs). The mRNA expressions of genetic markers of osteoclasts including TRAP and cathepsin K were significantly decreased in the presence of NOB-PEG-PCL. In addition, the NOB-PEG-PCL inhibited OC differentiation of BMMs through RANKL-induced MAPK signal pathway. After administration of the NOB-PEG-PCL, NOB-PEG-PCL prevented bone loss and improved bone density in OVX mice. These findings suggest that NOB-PEG-PCL might have great potential in the treatment of osteoporosis. The results suggested that NOB-PEG-PCL micelles could effectively prevent NOB fast release from micelles and extend circulation time. The NOB-PEG-PCL delivery system may be a promising way to prevent and treat osteoporosis.

The doublesex and mab-3 related transcription factor (DMRT) gene family involvement in sex development is widely conserved from invertebrates to humans. In this study, we identified a DM (Doublesex/Mab-3)-domain gene in Macrobrachium nipponense, which we named MniDMRT11E because it has many similarities to and phylogenetically close relationships with the arthropod DMRT11E. Amino acid alignments and structural prediction uncovered conservation and putative active sites of the DM domain. Real-time PCR analysis showed that the MniDMRT11E was highly expressed in the ovary and testis in both males and females. Cellular localization analysis showed that DMRT11E was mainly located in the oocytes of the ovary and the spermatocyte of the testis. During embryogenesis, the expression level of MniDMRT11E was higher at the cleavage stage than at other stages. During the different stages of ovarian development, MniDMRT11E expression gradually increased from OI to OIII and decreased to the lowest level at the end of OIV. The results indicated that MniDMRT11E probably played important roles in embryonic development and sex maturity in M. nipponense. MniDMRT11E dsRNA injection also significantly reduced vitellogenin (VG) expression and significantly increased insulin-like androgenic gland factor (IAG) expression, indicating a close relationship in gonad development.

Numerous studies focusing on physical activity in the context of thyroid cancer have been reported in recent years. However, there is no bibliometric analysis in this research field. This study aims to provide a comprehensive overview of the knowledge structure and research hotspots of physical activity in thyroid cancer through bibliometrics. Publications related to physical activity in thyroid cancer were searched in the Web of Science Core Collection (WoSCC) and PubMed databases from January 1, 2001 to May 31, 2025. VOSviewers, CiteSpace and R package \"bibliometrix\" were used to conduct bibliometric analysis. This bibliometric analysis spanned from 2001 to 2025 and involved 1020 authors from 182 institutions across 46 countries who contributed to 173 papers in 111 academic journals on physical activity in thyroid cancer. There has been an exponential growth trend in research on thyroid cancer and physical activity since 2009, with the United States leading in terms of publication volume. The United States dominated both in publications and citations. The journal led in both the number of publications and the frequency of citations. Studies predominantly focused on foundational clinical topics, with recent trends shifting towards metabolic health and patient-centered outcomes, reflecting global priorities in cancer survivorship care. This bibliometric study charts the evolution of thyroid cancer and physical activity research toward metabolic health and patient-centered outcomes. The assembled body of evidence conclusively links physical activity to a lower disease risk and better clinical outcomes, highlighting its significant public health and clinical implications.

BackgroundThe novel coronavirus disease 2019 (COVID-19) pandemic is still spreading across the world. Although the pandemic has an all-round impact on medical work, the degree of its impact on endovascular thrombectomy (EVT) for patients with acute ischemic stroke (AIS) is unclear.MethodsWe continuously included AIS patients with large artery occlusion who underwent EVT in a comprehensive stroke center before and during the Wuhan shutdown. The protected code stroke (PCS) for screening and treating AIS patients was established during the pandemic. The efficacy and safety outcomes including the rate of successful reperfusion (defined as modified Thrombolysis In Cerebral Infarction (mTICI) graded 2b or 3) and time intervals for reperfusion were compared between two groups: pre-pandemic and pandemic.ResultsA total of 55 AIS patients who received EVT were included. The baseline characteristics were comparable between the two groups. The time from hospital arrival to puncture (174 vs 125.5 min; p=0.002) and time from hospital arrival to reperfusion (213 vs 172 min; p=0.047) were significantly prolonged in the pandemic group compared with the pre-pandemic group. The rate of successful reperfusion was not significantly different between the two groups (85.7% (n=18) vs 88.2% (n=30); OR 0.971, 95% CI 0.785 to 1.203; p=1.000).ConclusionThe results of this study suggest a proper PCS algorithm which combines the COVID-19 screening and protection measures could decrease the impact of the disease on the clinical outcomes of EVT for AIS patients to the lowest extent possible during the pandemic.

Due to material plastic deformation and current leakage at high temperatures, SOI (silicon-on-insulator) and SOS (silicon-on-sapphire) pressure sensors have difficulty working over 500 °C. Silicon carbide (SiC) is a promising sensor material to solve this problem because of its stable mechanical and electrical properties at high temperatures. However, SiC is difficult to process which hinders its application as a high-temperature pressure sensor. This study proposes a piezoresistive SiC pressure sensor fabrication method to overcome the difficulties in SiC processing, especially deep etching. The sensor was processed by a combination of ICP (inductive coupled plasma) dry etching, high-temperature rapid annealing and femtosecond laser deep etching. Static and dynamic calibration tests show that the accuracy error of the fabricated sensor can reach 0.33%FS, and the dynamic signal response time is 1.2 μs. High and low temperature test results show that the developed sensor is able to work at temperatures from −50 °C to 600 °C, which demonstrates the feasibility of the proposed sensor fabrication method.

The Juno mission, launched in 2011, has significantly advanced our understanding of Jupiter's gravitational field, the solar wind and interplanetary conditions along its orbit, the evolution of Jupiter's high‐latitude magnetosphere and aurorae, the dynamics of plasma and electromagnetic fields in its magnetosphere, etc. As a result, new requirements for the definition and improvement of existing coordinate systems have arisen due to engineering implementation and the understanding of various scientific questions that rely on different preferred coordinate systems. In this paper, we first review the primary Jupiter's coordinate systems, including newly defined systems, such as the Jupiter Solar MAGnetosphere (JSMAG) and Jupiter Equatorial Inertial (JEIJ2000) coordinate systems, as well as a precisely defined orthogonal Jupiter Heliospheric (JH) coordinate system. We have also improved several magnetic field‐related coordinate systems and these systems support different Jupiter magnetic field models. The angular deviation of solar position‐related coordinate systems oriented by the semi‐analytical ephemerides TOP2013 is less than 70 milliarcseconds during the time span from 1800 to 2200 year. In addition, we propose a relatively simple, fast, and accurate transformation method, addressing the complexity of traditional coordinate system transformations entirely based on rotation axes and angles. This method introduces a basic coordinate system in which the unit vectors of all coordinate axes are represented. Finally, the comparison of our calculation with the published data indicates the high efficiency and accuracy of this work, which can be used as a basic tool for future Jovian system explorations from 1800 to 2200 year. Plain Language Summary Coordinate systems and their transformations play a critical role in both engineering and scientific research. Without appropriate coordinate systems, it would be difficult to accurately design spacecraft orbits, analyze scientific data, and understand physical mechanisms in different regions. Jupiter's coordinate systems, which are typically defined using astronomical basic vectors, can be challenging for researchers without an astronomy background. Moreover, traditional and fully Euler angle‐based rotation can be cumbersome for transforming between two undirectly related coordinate systems. Therefore, the development of a comprehensive set of Jupiter's coordinate systems based on recent research findings and the proposal of a universal and efficient transformation method can greatly facilitate deep space exploration and serve as a fundamental tool for studying the Jovian system. Key Points A comprehensive overview is provided of eight primary Jupiter's coordinate systems, some of which are newly defined or updated An efficient transformation method is proposed, in which unit vectors of all coordinate axes are represented in a basic coordinate system Comparison of our results with published data shows the high efficiency and accuracy of this work as a basic tool from 1800 to 2200 year

Background Breast cancer (BC) is the most prevalent malignancy in women. Potential therapeutic targets for BC are of great significance. In our previous study, we found that prenylated rab acceptor 1 domain family member 2 (PRAF2) is an oncogene in BC. However, the exact mechanism of PRAF2 in BC cancer promotion is still not fully understood. Methods Pan-cancer analysis of PRAF2 was performed in the TIMER, Kaplan‒Meier, UALCAN and GEPIA databases.The prognostic value of PRAF2 in BC was investigated in the GEPIA database. The influence of PRAF2 on immune infiltration in BC was analyzed in the TISIDE and TIMER databases. Finally, we validated the expression of PRAF2 in our institutional samples. After downregulating PRAF2 in two BC cell lines, we tested cell proliferation by CCK-8 and Wound healing assays. Results PRAF2 was highly expressed in various cancers, including BC, and in most BC cell lines. Higher expression of PRAF2 indicated poorer overall survival (OS) but not disease-free survival (DFS). Higher expression of PRAF2 is an independent prognostic factor in BC.PRAF2 is more highly expressed in BC than in the corresponding normal tissues. Downregulation of PRAF2 in BC can significantly inhibit viability and migration. Conclusions PRAF2 is highly expressed in various cancers, including BC. The expression of PRAF2 is related to Liquid–Liquid Phase Separation in BC. Finally, PRAF2 is upregulated in BC based on our institutional data. Downregulation of PRAF2 significantly inhibits cellular viability、migration in BC. PRAF2 may be a potential biomarker and therapeutic target for BC.