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Background Titanium ions can significantly promote plant growth and increase crop yield, yet the underlying mechanism remains an enigma. This study investigated the effect of titanium ions on tomato ( Solanum lycopersicum ) by foliar spraying and root irrigation with titanium ion solution (8 mg/L) in both pot and field trials. Results Pot and field trials showed that exogenous titanium ions significantly stimulated plant growth and root system development, enhanced antioxidant capacity, alleviated Blossom end rot and saline-alkali stress. After 24 h foliar application, comparative RNA-Seq profiling of leaves identified 4,214 differentially expressed genes. GO and KEGG enrichment analyses revealed that titanium ions affected core pathways such as DNA replication, protein translation, phenylpropanoid biosynthesis, plant-pathogen interaction, and plant hormone signal transduction in tomato. Notably, virtually all the genes associated with DNA replication and ribosome translation were remarkably upregulated. Other important upregulated genes included the phytohormone related genes (e.g., auxin, ethylene and gibberellin) and stress-related transcription factor genes (e.g., MYB13 , WRKY46 , WRKY51 , WRKY54 , PLT2 , ANT , and CRF2 ). The qRT-PCR validated the RNA-Seq results with high concordance. Conclusions This study partially elucidates the mechanisms by which titanium ions promote plant growth and enhance abiotic stress resilience, providing guidance for the application of titanium ion-based biostimulants in agricultural production.

Background Spur, a structure capable of producing and storing nectar, not only plays a vital role in the pollination process but also promotes the rapid diversification of some plant lineages, which is considered a key innovation in plants. Spur is the focus of many studies, such as evolution and ecological hypothesis, but the current understanding of spur development is limited. High-throughput sequencing of Impatiens uliginosa was carried out to study the molecular mechanism of its spur development, which is believed to provide some insights into the spur development of Impatiens . Results Transcriptomic sequencing and analysis were performed on spurs and limbs of I. uliginosa at three developmental stages. A total of 47.83 Gb of clean data were obtained, and 49,716 unigene genes were assembled. After comparison with NR, Swiss-Prot, Pfam, COG, GO and KEGG databases, a total of 27,686 genes were annotated successfully. Through comparative analysis, 19,356 differentially expressed genes were found and enriched into 208 GO terms and 146 KEGG pathways, among which plant hormone signal transduction was the most significantly enriched pathway. One thousand thirty-two transcription factors were identified, which belonged to 33 TF families such as MYB, bHLH and TCP. Twenty candidate genes that may be involved in spur development were screened and verified by qPCR, such as SBP, IAA and ABP. Conclusions Transcriptome data of different developmental stages of spurs were obtained, and a series of candidate genes related to spur development were identified. The importance of genes related to cell cycle, cell division, cell elongation and hormones in spur development was clarified. This study provided valuable information and resources for understanding the molecular mechanism of spur development in Impatiens .

Titanium ions significantly promote plant growth, but the mechanism is still unclear. Cut flowers are ideal materials for the study of plant growth and senescence. In this study, freshly cut Gerbera jamesonii were used to study the effects of titanium ions (8 mg/L) on the flower longevity. Flowering observation showed that the gerbera vase life was significantly prolonged in the presence of titanium ions. Plate colony counts showed that the amounts of bacteria in the vase solution of the control group were approximately 1700 times more than that of titanium ion treatment group. High-throughput sequencing was used to determine the sequences of 16S rRNA gene V3-V4 variable regions of the vase solutions to analyze bacterial species, their average proportions, and absolute abundance. The results showed that the titanium ions reduced the entire bacterial counts as well as altered the absolute abundance of different bacterial species in the vase solution. The most prevalent bacteria were mainly Enterobacteriaceae, Pseudomonas veronii, Pseudomonas sp., Delftia sp., Agrobacterium sp., Sphingobacterium multivorum, Acinetobacter johnsonii, and Clostridiaceae. In combination with plate colony counts, we demonstrated that all the bacterial growths were significantly inhibited by titanium ions, regardless of their average proportions increased or decreased. These results showed that titanium ions could extend effectively the longevity of gerberas and possess the broad-spectrum antibacterial properties. This study provides a basis for further mechanism exploration of titanium ions action and its applications in cut flower preservation and agricultural production.

Background Research has associated human epidermal growth factor receptor (HER2) with glucose and lipid metabolism. However, the association between circulating HER2 levels and coronary artery disease (CAD) remains to be elucidated. Methods We performed a case–control study with 435 participants (237 CAD patients and 198 controls) who underwent diagnostic coronary angiography from September 2018 to October 2019. Adjusted odds ratios (ORs) and 95% confidence intervals (CIs) for CAD were calculated with multiple logistic regression models after adjustment for confounders. Results Overall, increased serum HER2 levels were independently associated with the presence of CAD (OR per 1-standard deviation (SD) increase: 1.438, 95% CI 1.13–1.83; P = 0.003) and the number of stenotic vessels (OR per 1-SD increase: 1.399, 95% CI 1.15–1.71; P = 0.001). In the subgroup analysis, a significant interaction of HER2 with body mass index (BMI) on the presence of CAD was observed (adjusted interaction P = 0.046). Increased serum HER2 levels were strongly associated with the presence of CAD in participants with BMI ≥ 25 kg/m 2 (OR per 1-SD increase: 2.143, 95% CI 1.37–3.35; P = 0.001), whereas no significant association was found in participants with BMI < 25 kg/m 2 (OR per 1-SD increase: 1.225, 95% CI 0.90–1.67; P = 0.201). Conclusion Elevated HER2 level is associated with an increased risk of CAD, particularly in people with obesity. This finding yields new insight into the pathological mechanisms underlying CAD, and warrants further research regarding HER2 as a preventive and therapeutic target of CAD.

The data and events from Internet of Things are mass but their semantic information is very simple and can not be utilized in upper applications directly, so we have to analyze lots of data to find out useful business logic in these events. To aggregate these simple basic events into advanced events, a hierarchical model was designed to process RFID data stream, in which a complex events processing (CEP) mechanism was used to analyze atomic events in RFID data. We defined event handling operators in CEP mechanism to process business logics. As an example, the CEP was used in a logistic application, which implemented logistic events aggregation and complex events processing effectively. The result indicates the CEP mechanism is an effective approach to handle data and events in Internet of Things.

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The advance of research on activated carbon loaded metal at home and abroad was depicted. The method and character of activated carbon loaded metal was also investigated systematically. Based on the sorption mechanism, it was briefly introduced that application on removal chromium from wastewater using modified activated carbon. Finally, the work pointed the exist problems that is how to reduce cost and optimize process, how to regenerate and reuse activated carbon, and how to increase utilization activated carbon.

The adsorption property of Cu2+ in aqueous solution by carbonaceous adsorbent made by sludge was studied. Under static conditions, the influences to the adsorption properties by the dosage of adsorbent, adsorption time, concentration of Cu2+, adsorption temperature, pH value, and other factors were systematically studied. The adsorption kinetics of the carbonaceous adsorbent was analyzed, and its adsorption properties were primarily discussed. The research results show that the carbonaceous adsorbent made by sludge has strong removal efficiency of Cu2+; the adsorption process complies with the Fruendlich adsorption isotherm; and the adsorption process can be represented by first-order kinetic model.

Background： The European Society of Urogenital Radiology has built the Prostate Imaging Reporting and Data System （PI-RADS） for standardizing the diagnosis of prostate cancer （PCa）. This study evaluated the PI-RADS diagnosis method in patients with prostate-specific antigen （PSA） 〈20 ng/ml. Methods： A total of 133 patients with PSA 〈20 ng/ml were prospectively recruited. T2-weighted （T2WI） and diffusion-weighted （DWI） magnetic resonance images of the prostate were acquired before a 12-core transrectal prostate biopsy. Each patient＇s peripheral zone was divided into six regions on the images; each region corresponded to two of the 12 biopsy cores. T2WI, DWI, and T2W1 ＋ DWI scores were computed according to PI-RADS. The diagnostic accuracy of the PI-RADS score was evaluated using histopathology of prostate biopsies as the reference standard. Results： PCa was histologically diagnosed in 169 （21.2%） regions. Increased PI-RADS score correlated positively with increased cancer detection rate. The cancer detection rate for scores 1 to 5 was 2.8%, 15.0%, 34.6%, 52.6%, and 88.9%, respectively, using T2W1 and 12.0%, 20.2%, 48.0%, 85.7%, and 93.3%, respectively, using DWI. For T2WI ＋ DWI, the cancer detection rate was 1.5% （score 2）, 13.5% （scores 3-4）, 41.3% （scores 5-6）, 75.9% （scores 7-8）, and 92.3% （scores 9-10）. The area under the curve for cancer detection was 0.700 （T2WI）, 0.735 （DWI） and 0.749 （T2WI ＋ DWI）. The sensitivity and specificity were 53.8% and 89.2%, respectively, when The summed score ofT2Wl ＋ DWI

We studied the allelic frequency distributions and statistical forensic parameters of 21 new short tandem repeat（STR）loci and the amelogenin locus,which are not included in the combined DNA index system（CODIS）,in a Russian ethnic minority group from the Inner Mongolia Autonomous Region,China.A total of 114 bloodstain samples from unrelated individuals were extracted and co-amplified with four fluorescence-labeled primers in a multiplex polymerase chain reaction（PCR）system.Using capillary electrophoresis,the PCR products of the 21 STR loci were separated and genotyped.A total of 161 alleles were observed in the Russian ethnic minority group,and corresponding allelic frequencies ranged from 0.0044 to 0.5965.The 21 non-CODIS STR loci of the Russian ethnic minority group were characterized by high genetic diversity and therefore may be useful for elucidating the population’s genetic background,for individual identification,and for paternity testing in forensic practice.