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"Wiley, Melissa"
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Inch and Roly make a wish
Roly Poly, Inchworm, Dragonfly, and Beetle all feel bored one sleepy afternoon, until Roly suggests they go to the wishing well to wish for something to do.
Book
Haplotype phasing of whole human genomes using bead-based barcode partitioning in a single tube
Haplotype information for whole genomes is rapidly generated with a single-tube method.
Haplotype-resolved genome sequencing promises to unlock a wealth of information in population and medical genetics. However, for the vast majority of genomes sequenced to date, haplotypes have not been determined because of cumbersome haplotyping workflows that require fractions of the genome to be sequenced in a large number of compartments. Here we demonstrate barcode partitioning of long DNA molecules in a single compartment using “on-bead” barcoded tagmentation. The key to the method that we call “contiguity preserving transposition” sequencing on beads (CPTv2-seq) is transposon-mediated transfer of homogenous populations of barcodes from beads to individual long DNA molecules that get fragmented at the same time (tagmentation). These are then processed to sequencing libraries wherein all sequencing reads originating from each long DNA molecule share a common barcode. Single-tube, bulk processing of long DNA molecules with ∼150,000 different barcoded bead types provides a barcode-linked read structure that reveals long-range molecular contiguity. This technology provides a simple, rapid, plate-scalable and automatable route to accurate, haplotype-resolved sequencing, and phasing of structural variants of the genome.
Journal Article
Fox and Crow are not friends
Continuing Aesop's fable, Fox and Crow tussle over pieces of cheese, but Mama, one of the three bears, outwits them both.
Book
Profound Autonomic Instability Complicated by Multiple Episodes of Cardiac Asystole and Refractory Bradycardia in a Patient with Anti-NMDA Encephalitis
Anti-N-methyl-d-aspartate receptor encephalitis (anti-NMDARE) is autoimmune encephalitis primarily affecting young adults and children. First described about a decade ago, it frequently manifests as a syndrome that includes progressive behavioral changes, psychosis, central hypoventilation, seizures, and autonomic instability. Although cardiac arrhythmias often accompany anti-NMDARE, the need for long-term electrophysiological support is rare. We describe the case of NMDARE whose ICU course was complicated by progressively worsening episodes of tachyarrhythmia-bradyarrhythmia and episodes of asystole from which she was successfully resuscitated. Her life-threatening episodes of autonomic instability were successfully controlled only after the placement of a permanent pacemaker during her ICU stay. She made a clinical recovery and was discharged to a skilled nursing facility after a protracted hospital course.
Journal Article
The prairie thief
In late nineteenth-century Colorado, Louisa's father is erroneously arrested for thievery and, while under the charge of the awful Smirch family, Louisa and a magical friend must find a way to prove his innocence.
Book
Everyone Has a Story Healing Macro Systems Through Micro Relationships
In 2020, Wiley's nation became indirect and aggressive communicators. From social media posts to yard signs, every person had an opinion. And often, those opinions took hold in passive declarations. They traded social media arguments for intimate connection and conversation. And in those moments of passive anger, they languished. They felt disconnected and hopeless. More sure of themselves and their own righteousness, but less sure of others. Every person she knows thinks they are right and a \"good person.\" Every person she knows also thinks that someone else is to blame for all that is wrong with the world.
Journal Article
The Pressure of Pretty
Women can do anything. As long as we're pretty. Not too pretty, but pretty enough. Because too pretty is threatening. I didn't make the rules and neither did you. We just live them. I consider myself an expert on beauty because I am facially disfigured. A childhood dog attack left me scarred and lopsided. And we can only understand the true power of something if we first experience its opposite. For example, what do we know of health if we've never been sick? What do we know of connection if we've never been lonely? Empathy isn't taught, it's lived. And I know the power of beauty because I've lived at the periphery of its force. Beauty isn't an achievement or contribution. It fades under seasons of stress, sickness, and age. It's a slippery alter at best and destructive at worst. It sends our children--especially our daughters--into the jaws of bullying, eating disorders, addiction, and suicide.
Trade Publication Article
Counterpoint: The IRS's First-Time Abatement Policy ... Even Harsher Than You Realized
Over the years, I've successfully used the policy to obtain penalty abatement for many relieved clients who thought they would either be stuck paying exorbitant penalties for an innocent mistake, or forced to pay a tax advisor's hourly rates to navigate the lengthy and inefficient reasonable cause abatement process. Given that Appeals will usually decline to review FTA denials on the grounds that such relief is not statutory, but rather a matter of administrative grace, there's good reason to make it as easy as possible for the Service Center reviewer to grant your FTA request in the first place.) Another common misconception is that taxpayers can \"save\" FTA for a future year if they qualify for reasonable cause relief. The flip side of FTA, which Mr. Brodeur's piece nicely points out, is that it is a time-saving freebie from the IRS that can save qualifying taxpayers penalties and the time and expense of hiring a practitioner to work through the reasonable cause process.
Journal Article
Murrin and Zuch provide insight into the limits of taxpayers' rights
Zuch then petitioned the Tax Court for relief. Because the IRS no longer had a reason to levy Zuch, it moved to dismiss the Tax Court case as moot, arguing that the Tax Court lacked jurisdiction over the case because the Service did not have a basis to levy on Zuch's property. [...]of the decision, Gorsuch wrote, Sec. 6330 proceedings \"are essentially risk-free for the IRS. In Vigon, the taxpayer had requested a CDP hearing after the Service had assessed penalties pursuant to Sec. 6702 and filed a notice of federal tax lien. [...]a refund suit is the least favored of the avenues available to taxpayers as a practical matter.) However, caution in this arena is worthy of consideration.
Trade Publication Article