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To assess the efficiency of seven treatments including biochars produced from dried faecal matter and manures as stabilizing agents of cadmium (Cd)-spiked soils, lettuce was grown in glasshouse on two contrasting soils. The soils used were moderately fertile silty loam and less fertile sandy loam and the applied treatments were 7 % w/w. The reduction of bioavailable Cd (ammonium nitrate extractable) and its phytoavailability for lettuce were used as assessment criteria in the evaluation of stabilization performance of each treatment. Moreover, the agronomic values of the treatments were also investigated. Ammonium nitrate extraction results indicated that faecal matter biochar, cow manure biochar and lime significantly reduced bioavailable Cd by 84–87, 65–68 and 82–91 %, respectively, as compared to the spiked controls. Unpredictably, coffee husk biochar induced significant increment of Cd in NH 4 NO 3 extracts. The immobilization potential of faecal matter biochar and lime were superior than the other treatments. However, lime and egg shell promoted statistically lower yield and P, K and Zn concentrations response of lettuce plants compared to the biochar treatments. The lowest Cd and highest P tissue concentrations of lettuce plants were induced by faecal matter and cow manure biochar treatments in both soils. Additionally, the greatest Cd phytoavailability reduction for lettuce was induced by poultry litter and cow manure biochars in the silty loam soil. Our results indicate that faecal matter and animal manure biochars have shown great potential to promote Cd immobilization and lettuce growth response in heavily contaminated agricultural fields.

Khat (Catha edulis) chewing is widespread in the region of East Africa. Even low levels of organochlorine pesticides (OCPs) in khat could induce public health concern. In a market-based study, from five popular khat varieties, a total of 35 composite khat samples were analyzed for dichlorodiphenyltrichloroethane (DDT) and its main transformation products, and four hexachlorocyclohexane (HCH) isomers. Extraction was carried out by quick, easy, cheap, effective, rugged and safe method (QuEChERS). OCP concentrations were determined by head space solid phase microextraction coupled to gas chromatography-mass spectrometry (HS-SPME–GC–MS). Every sample contained β-HCH above the maximum residue limit set by the European Commission. For total DDT, this was the case for 25.7% of the samples. The ratios of (p,p′-DDD + p,p′-DDE) to p,p′-DDT were less than one for 85% of khat samples, demonstrating recent use of DDT in khat farmlands. Conversely, the ratio of β-HCH to total HCH varied from 0.56 to 0.96, implying historical input of technical HCH. Assuming a daily chewable portion of 100 g, dietary intakes of p,p′-DDT, total DDT and total HCH by adults ranged from 3.12 to 57.9, 6.49 to 80.2 and 39.2 to 51.9 ng (kg body weight)−1 day−1, respectively. These levels are below acceptable levels suggested by international organizations. Chewing khat showed lower non-cancer health risk, but showed relatively higher cancer risk in terms of OCPs. Because khat is chewed without being subjected to any treatment, uncertainties associated with estimated intakes and health risks should be low. Therefore, this practice is of great concern.

The use of wastewater to produce food crops particularly vegetables is very prevalent in Addis Ababa, Ethiopia. This practice may pose health risks to farm workers and consumers. Hence, the study was designed to evaluate farmers’ perceptions on irrigation water quality, health risks and health risk mitigation measures in four wastewater-irrigated urban vegetable farming sites in Addis Ababa. Data were collected on farm through 263 individual interviews and 12 focus group discussions. The findings showed that despite differences in levels of knowledge and awareness on health risks, farmers appear informed about the contamination of their irrigation water. The difference in perception to quality consideration of Akaki River/irrigation water is highlighted by the result of Kruskal–Wallis H test analysis which shows significant mean value (1.33) of positive perception toward the water quality by male than female farmers. Interestingly, significant difference (p < 0.05) in mean values of awareness toward problems of eating unwashed vegetables is also found between male and female farmers where females seemed to be more aware. Conversely, no significant difference was found in mean value of perception and awareness toward vegetables quality. Among the perceived health risks, skin problems were top-rated health risk while eye burn, sore feet and abdominal pains were rated low across the four farming sites. Although statistically not significant, perception toward consumption-related health risk differed with gender: females assigned relatively high mean score. Irrespective of the farming site and gender differences, the most accepted health risk reduction measures were health promotion programs and cessation of irrigation before harvesting. In view of crop restriction measures, females assigned significantly (p = 0.044) low mean score to planting non-food produce. Akaki-Addis farmers suitability perceptions of planting non-food produce and non-raw eaten crops were significantly (p < 0.001) higher than the other farming sites. Therefore, effective site and gender-specific educational programs have the potential for clarifying farmers and consumers’ risks and risk management perceptions and improving practical knowledge, which in turn may help identify adoption barriers, opportunities and incentives.

Micronutrient deficiency remains an enormous problem in refugee settings. Transforming refugees’ food systems through the scaling up of kitchen gardening and fortifying relief food crops with minor food components including nutrient-dense spices can help improve the quality of staple foods. Globally, spices are indispensable in the daily diet and play an important role in the socio-cultural setting of different communities. Forty turmeric and curry powder samples were collected from different market establishments and geographic locations in East Africa. The samples were analyzed for selected elements using Portable X-Ray Fluorescence (PXRF). The contents of potassium (K), calcium (Ca), zinc (Zn) and strontium (Sr) in turmeric powder were statistically different among geographic origins (Ethiopia, Kenya and Uganda). We also aimed to determine if a small portion of spices (turmeric (5 g) and curry (4 g)) would contribute to an adequate intake (AI) or recommended dietary allowance (RDA) for selected minerals, for refugee men and women aged between 19 and 50 years as defined by the Food and Nutrition Board of the Institute of Medicine (IOM). For the reference groups, the contributions of turmeric and curry powder to AI/RDA for K, Ca and Zn varied between 0.48 to 4.13%. On the other hand, turmeric was identified to contribute > 20% AI/RDA for refugee men and women aged between 19 and 50 years for two micro minerals: manganese (Mn) and iron (Fe). Considering turmeric and curry powder of East African origins are good sources of minerals and present acceptable toxic metal(loid)s loads coupled with low cost, these spices particularly turmeric should be more widely popularized and recommended for food-to-spice fortification among the refugee population located in East Africa. Highlights Information concerning the mineral contents of spices is lacking. Spices are a good source of several minerals and contribute to dietary intakes of nutritionally important minerals. Nutrition intervention, including food-to-spice fortification for improving mineral intakes by refugee community, is required.

Background Wastewater irrigation for vegetable production is a highly prevalent practice in Addis Ababa and a number of articles have been published on wastewater-irrigated soils and vegetables contaminated with heavy metals. However, to the best of our knowledge, an insight into assessment of human health risks associated with the consumption of vegetable crops grown on wastewater-irrigated soils is non-existent in the city. Long-term effect of wastewater irrigation on the build-up of heavy metals in soils and selected vegetable crops in Addis Ababa urban vegetable farming sites (10) was evaluated. By calculating estimated daily intakes (EDIs) and target hazard quotients (THQs) of metals, health risk associated with the consumption of the analyzed vegetables was also evaluated. Results The heavy metal concentrations in irrigation water and soils did not exceed the recommended maximum limits (RMLs). Moreover, Cd, Co, Cr, Cu, Ni and Zn concentrations in all analyzed vegetables were lower than the RML standards. In contrast, Pb concentrations were 1.4–3.9 times higher. Results of two way ANOVA test showed that variation in metals concentrations were significant ( p  < 0.001) across farming site, vegetable type and site x vegetable interaction. The EDI and THQ values showed that there would be no potential health risk to local inhabitants due to intake of individual metal if one or more of the analyzed vegetables are consumed. Furthermore, total target hazard quotients (TTHQs) for the combined metals due to all analyzed vegetables were lower than 1, suggesting no potential health risk even to highly exposed local inhabitants. Conclusions There is a great respite that toxic metals like Pb and Cd have not posed potential health risk even after long term (more than 50 years) use of this water for irrigation. However, intermittent monitoring of the metals from irrigation water, in soil and crops may be required to follow/prevent their build-up in the food chain.

Kitchen gardening is considered a way to reconnect with agriculture and complement the cereal-based relief food offered to refugees in East Africa. This work aimed at profiling mineral content of okra in four refugee camps and settlements located in Ethiopia and Uganda and its contribution to adequate intake (AIs) or recommended dietary allowances (RDAs) for young children and pregnant and lactating women (PLW). The study also evaluated the applicability of portable X-ray fluorescence (PXRF) as compared with inductively coupled plasma mass spectrometry (ICP-MS) for mineral profiling of okra powder samples. The contents of minerals (mg kg −1 ) from the ICP-MS readings were in the following ranges: K (14,385–33,294), Ca (2610–14,090), P (3178–13,248), Mg (3896–7986), Cu (3.81–19.3), Fe (75.7–1243), Zn (33–141) and Mn (23.1–261). Regardless of geographic origin, at low-end consumption probability (17 g day −1 for young children and 68 g day −1 for PLW), okra could contribute ˂ 15% (2.7–12.9%) AI for macro-minerals (K and Ca). In addition, the contributions to RDA values for Fe and Zn, elements of known public health interest, ranged from 4.5 to 34.7% for young children. Interestingly, regression lines revealed strong agreement between ICP-MS and PXRF readings for Mn and Zn, with R 2 values > 0.91. This information is useful in support of nutrition-sensitive kitchen gardening programs through scaling culturally important crops in refugee settings.

Among the most renowned Ethiopian food crops, teff (Eragrostis tef (Zucc.)Trotter) is the most nutritious and gluten-free cereal. Because of the increase in demand for teff, it is necessary to establish geographic origin authentication of traditional teff brands based on multi-element fingerprint. For this purpose, a total of 60 teff samples were analysed using Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Accuracy of the laboratory procedure was verified by the analysis of rice flour standard reference material (NIST SRM 1568b). In this context, four traditional teff brands (Ada’a, Ginchi, Gojam and Tulu Bolo) were analytically characterized using multi-element fingerprint and further treated statistically using linear discriminant analysis (LDA). Due to obvious extrinsic Fe, Al and V contamination, these elements were excluded from the discriminant model. Five elements (Cu, Mo, Se, Sr, and Zn) significantly contributed to discriminate the geographical origin of white teff. On the other hand, Mn, Mo, Se and Sr were used as discriminant variables for brown teff. LDA revealed 90 and 100% correct classifications for white and brown teff, respectively. Overall, multi-element fingerprint coupled with LDA can be considered a suitable tool for geographic origin discrimination of traditional teff brands.

Background In Addis Ababa, where irrigation water for vegetable production is commonly derived from the highly polluted Akaki river, information on microbial contamination of water and irrigated vegetable is scanty. An assessment was done to determine the microbiological quality of irrigation water and lettuce harvested from 10 urban farming sites of Addis Ababa. The efficacy of 5 lettuce washing methods were also assessed. A total of 210 lettuce and 90 irrigation water samples were analyzed for faecal coliform and helminth eggs population levels. Results The mean faecal coliform levels of irrigation water ranged from 4.29-5.61 log 10 MPN 100 ml −1 , while on lettuce, the concentrations ranged from 3.46-5.03 log 10 MPN 100 g −1 . Helminth eggs and larvae were detected in 80% of irrigation water and 61% of lettuce samples. Numbers ranged from 0.9-3.1 eggs 1000 ml −1 and 0.8-3.7 eggs 100 g −1 wet weight for irrigation water and lettuce, respectively. The helminth eggs identified included those of Ascaris lumbricoides , Hookworm, Enterobius vermicularis , Trichuris trichiura , Taenia and Strongloyides larvae. Ascaris lumbricoides and Hookworm were most prevalent in both irrigation water and lettuce samples. Compared with the WHO recommendations and international standards, the faecal coliform and helminth eggs levels in irrigation water and lettuce samples exceeded the recommended levels. Irrespective of the tested washing methods, faecal coliform and helminth eggs levels were somehow reduced. Among the washing methods, potable tap water washing - rinsing (2 min) followed by dipping in 15 000 ppm vinegar solution for a minute supported the highest faecal coliorm reduction of 1.7 log 10 units, whereas lowest reduction of 0.8 log 10 units was achieved for the same procedure without vinegar. Conclusion Compared with international standards, both faecal coliform and helminth eggs levels exceeded recommended thresholds in water and lettuce, but still in a potential risk range which can be easily mitigated if farmers and households are aware of the potential risk. Aside preventing occupational exposure, potential risk reduction programs should target households which have so far no guidance on how best to wash vegetables. The result of the present study suggest that the vinegar based washing methods are able to reduce faecal coliform towards low level while the physical washing with running water may help to substantially decrease potential risk of helminth parasitic infections.

Concentrations of metal(loid)s, Ag, Al, As, Cd, Co, Cr, Cu, Fe, Mn, Ni, Se, Sr, V and Zn, were determined in rice on sale in Las Vegas. The rice samples were grown in five different countries, the USA, Thailand, India, Pakistan, and Bangladesh. The elemental concentrations in rice grain were determined using inductively coupled plasma mass spectrometry (ICP-MS) following hot block-assisted digestion. The accuracy of the laboratory procedure was verified by the analysis of rice flour standard reference material (NIST SRM 1568b). The mean metal(loid) contents in rice of various geographic origins were 3.18–5.91 mg kg−1 for Al, 0.05–0.12 mg kg−1 for As, 3.64–41 μg kg−1 for Cd, 5.11–12 μg kg−1 for Co, 0.12–0.14 mg kg−1 for Cr, 1.5–1.91 mg kg−1 for Cu, 3.04–4.98 mg kg−1 for Fe, 4.2–10.4 mg kg−1 for Mn, 0.21–0.41 mg kg−1 for Ni, 0.02–0.07 mg kg−1 for Se, 0.68–0.88 mg kg−1 for Sr, 3.64–5.26 μg kg−1 for V, and 16.6–19.9 mg kg−1 for Zn. respectively. The mean concentration of As in US rice was significantly higher than in Indian, Pakistani, and Bangladeshi rice. On the other hand, it was found a significantly low mean level of Cd in US-grown rice. It was also found that the concentrations of metal(loid)s in black and brown rice on sale in Las Vegas were statistically similar, except for Mn and Se. The geographic origin traceability of rice grain involved the use of ICP-MS analysis coupled with chemometrics that allowed their differentiation based on the rice metal(loid) profile, thus confirming their origins. Data were processed by linear discriminant analysis, and US and Thai rice samples were cross-validated with higher accuracy (100%). This authentication quickly discriminates US rice from the other regions and adds verifiable food safety measures for consumers.

The traditional Ethiopian flat bread, injera, is a regular component of daily diets in Ethiopia and Eritrea. This bread is also popular among urban refugees particularly Eritreans in Addis Ababa. The levels of metal(loid)s in 40 composite (120 sub-samples) injera samples, representing 4 types of market establishments in Addis Ababa, were determined using inductively coupled plasma-mass spectrometry (ICP-MS) and portable X-ray fluorescence (PXRF). For ICP-MS analysis, the accuracy of the method was evaluated by the analysis of a certified reference material and recovery experiments. It was found that the correlations between the mean levels of Al and Fe and between Al and Mn in injera were highly significant ( p  < 0.001). It was also found that 1.5 fresh injeras would cover 48–75% of recommended dietary allowance (RDA) for Mg, 17–21% of RDA for K, 19–23% of RDA for Ca, and 60–72% of RDA for P for an adult group aged between 19 and 50. Daily intakes of Al, Fe, and Mn were found to be above the provisional tolerable daily intake (PTDI)/maximum tolerable daily intake (MTDI) values. The mean target hazard quotient (THQ) values for Fe and Mn were greater than 1. The total THQ values varied from 6.52 to 8.53 among market establishments. Estimating carcinogenic risk due to exposure to As, Cr, and Pb indicated that perennial injera consumers might remain at cancer risk. This would further escalate if other staple food items and spices are considered. Hence, there is a need for home-based strategies to reduce extrinsic soil-Al-Fe-Mn in injera/tef batter.