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Ethics and Integrity in Public Administration presents cutting-edge perspectives on the role of ethics in public sector management - what it is and where it is going. The contributors include a cross-section of authoritative authors from around the globe, and from both the academy and government. They cover a wide range of topics, diverse theoretical and conceptual paradigms, and global examples, and provide a broader view than what is typically offered in other books. The book includes both theoretical insights and commentaries grounded in practice. Chapters are divided into three parts: Ethical Foundations and Perspectives, Ethical Management and Ethical Leadership, and International and Comparative Perspectives.

CRISPR-Cas genome editing induces targeted DNA damage but can also affect off-target sites. Current off-target discovery methods work using purified DNA or specific cellular models but are incapable of direct detection in vivo. We developed DISCOVER-Seq (discovery of in situ Cas off-targets and verification by sequencing), a universally applicable approach for unbiased off-target identification that leverages the recruitment of DNA repair factors in cells and organisms. Tracking the precise recruitment of MRE11 uncovers the molecular nature of Cas activity in cells with single-base resolution. DISCOVER-Seq works with multiple guide RNA formats and types of Cas enzymes, allowing characterization of new editing tools. Off-targets can be identified in cell lines and patient-derived induced pluripotent stem cells and during adenoviral editing of mice, paving the way for in situ off-target discovery within individual patient genotypes during therapeutic genome editing.

Reintroduction of imperiled native freshwater fish is becoming an increasingly important conservation tool amidst persisten anthropogenic pressures and new threats related to climate change. We summarized trends in native fish reintroductions in the current literature, identified predictors of reintroduction outcome, and devised recommendations for managers attempting future native fish reintroductions. We constructed random forest classifications using data from 260 published case studies of native fish reintroductions to estimate the effectiveness of variables in predicting reintroduction outcome. The outcome of each case was assigned as a success or failure on the basis of the author's perception of the outcome and on whether or not survival, spawning, or recruitment were documented during post-reintroduction monitoring. Inadequately addressing the initial cause of decline was the best predictor of reintroduction failure. Variables associated with habitat (e.g., water quality, prey availability) were also good predictors of reintroduction outcomes, followed by variables associated with stocking (e.g., genetic diversity of stock source, duration of stocking event). Consideration of these variables by managers during the planning process may increase the likelihood for successful outcomes in future reintroduction attempts of native freshwater fish. La reintroducción de peces nativos de agua dulce que se encuentran en peligro se está convirtiendo cada vez más en una herramienta importante de conservación frente a las presiones antropogénicas persistentes y nuevas amenazas relacionadas con el cambio climático. Resumimos las tendencias encontradas en la literatura actual sobre la reintroducción de peces nativos, identificamos pronosticadores de resultados de la reintroducción e ideamos recomendaciones para administradores que intenten reintroducciones de peces nativos en el futuro. Construimos clasificaciones de bosque aleatorio a partir de datos de 260 estudios de caso publicados sobre la reintroducción de peces nativos para estimar la efectividad de las variables en la predicción del resultado de la reintroducción. El resultado de cada caso fue asignado como un éxito o un fracaso con base en la percepción del autor a partir del resultado y dependiendo de si se documentó o no la supervivencia, el desove o el reclutamiento durante el monitoreo posterior a la reintroducción. Abordar inadecuadamente a la causa inicial de la declinación fue el mejor pronosticador del fracaso de la reintroducción. Las variables asociadas con el hábitat (p. ej.: calidad del agua, disponibilidad de la presa) también fueron buenos pronosticadores de los resultados de la reintroducción, seguidas por las variables asociadas con el stock (p. ej.: la diversidad genética de la fuente del stock, duración del evento de stock). Que los administradores consideren estas variables durante el proceso de planeación puede incrementar la probabilidad de resultados exitosos en futuros intentos de reintroducción de peces nativos de agua dulce.