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Anthracnose is the most devastating disease affecting persimmon (Diospyros kaki Thunb.) cultivars in China, and is caused by the pathogen recently identified as Colletotrichum horii. In this study, the symptoms of anthracnose in persimmon fruits and trees in China, and the morphological characteristics of C. horii, were observed and recorded. Thirty simple sequence repeat (SSR) markers for C. horii were developed from the whole genome of the closely related species C. gloeosporioides, which is available from the GenBank database. After screening 30 combinations of primer pairs, six SSR markers were selected to amplify the genomic DNA of 23 isolates, to assess the degree of polymorphism and the reproducibility of the SSR markers. A total of 164 discernible bands were obtained when visualizing the amplicons by electrophoresis, 156 of which showed polymorphism. A dendrogram was constructed with FreeTree software (ver. 0.9.1.50) and the similarity coefficients ranged from 0.58–0.98. The result indicated there was genetic diversity in the C. horii population in China. There was an obvious correlation between the cluster groups and the sites from which the samples were isolated. The results of this study will facilitate a more detailed understanding of the population structure of C. horii in China.

[Objective]This study was designed to screen the fungicides for controlling pathogens of persimmon anthracnose by laboratory and field test. [Method]4 representative persimmon anthracnose strains collected from Qingzhou of Shandong Province,Gongcheng of Guangxi Zhuang Autonomous Region,Luanchuan of Henan Province and Fuping of Shanxi Province,were selected as the trial materials. By comparing the minimum inhibitory concentration( MIC) value and the concentration for 50% of maximal effect( EC50) value of 22 kinds of fungicides belonging to 10 categories to decide the inhibition activity of each fungicide. 5 kinds of fungicide were finally selected to conduct field trials. [Result]The results show that: benzimidazole,demethylation inhibitor and benzene- pyrrole acted better,the MIC values were below 10 mu g times m L super(-1),and the EC50 within the scope of 0. 011 2- 0. 520 3 mu g times m L super(-1). Field trials demonstrated that the inhibition efficacy of 30% difenoconazole WG( 1500 x),240 g times L super(

Based on the land utilization data, the distribution pattern of forest green-land with different area and types in Nanyang City, Henan Province were analyzed. The result showed that (1) the area of forest was relatively small in Nanyang City; (2) the patches of forest lands were fragmented and distributed un-uniformly; and (3) the density and average area of forest patched varied greatly. Enforcing large-scale vegetation patches construction and small patches connection will be the emphasis of urban forest construction in the future.

Timely removal of oxidatively damaged proteins is crit- ical for cells exposed to oxidative stresses; however, cellular mechanism for clearing oxidized proteins is not clear. Our study reveals a novel type of protein modifi- cation that may play a role in targeting oxidized proteins and remove them. In this process, DSS1 （deleted in split hand/split foot 1）, an evolutionally conserved small protein, is conjugated to proteins induced by oxidative stresses in vitro and in vivo, implying oxidized proteins are DSS1 clients. A subsequent ubiquitination targeting DSSl-protein adducts has been observed, suggesting the client proteins are degraded through the ubiquitin- proteasome pathway. The DSS1 attachment to its clients is evidenced to be an enzymatic process modulated by an unidentified ATPase. We name this novel protein modification as DSSylation, in which DSS1 plays as amodifier, whose attachment may render target proteins a signature leading to their subsequent ubiquitination, thereby recruits proteasome to degrade them.

A sampling campaign including summer, autumn and winter of 2014 and spring of 2015 was accomplished to obtain the characteristic of chemical components in PM2.5 at three sites ofKunming, a plateau city in South-west China. Nine kinds of water-soluble inorganic ions （WSI）, organic and element carbon （OC and EC） in PM2.5 were analyzed by ion chromatography and thermal optical reflectance method, respectively. Results showed that the average concentrations of total WSI, OC and EC were 22.85±10.95 μg.m -3, 17.83±9.57 μg.m-3 and 5.114-4.29 μg.m-3, respectively. They totally accounted for 53.0% of PM2.5. Secondary organic and inorganic aerosols （SOA and SIA） were also assessed by the minimum ratio of OC/EC, nitrogen and sulfur oxidation ratios. The annual average concentrations of SOA and SIA totally accounted for 28.3% of the PM2.5 concentration. The low proportion suggested the primary emission was the main source of PM2.5 in Kunming. However, secondary pollution in the plateau city should also not be ignorable, due to the appropriate temperature and strong solar radiation, which can promote the atmospheric photochemical reactions.

Microglia play a key role in regulating synaptic remodeling in the central nervous system. Activation of classical complement pathway promotes microglia-mediated synaptic pruning during development and disease. CD47 protects synapses from excessive pruning during development, implicating microglial SIRPα, a CD47 receptor, in synaptic remodeling. However, the role of microglial SIRPα in synaptic pruning in disease remains unclear. Here, using conditional knock-out mice, we show that microglia-specific deletion of SIRPα results in decreased synaptic density. In human tissue, we observe that microglial SIRPα expression declines alongside the progression of Alzheimer’s disease. To investigate the role of SIRPα in neurodegeneration, we modulate the expression of microglial SIRPα in mouse models of Alzheimer’s disease. Loss of microglial SIRPα results in increased synaptic loss mediated by microglia engulfment and enhanced cognitive impairment. Together, these results suggest that microglial SIRPα regulates synaptic pruning in neurodegeneration. Microglial SIRPα regulates synaptic pruning during development. Its role in neurodegeneration is unclear. Here, the authors show microglial SIRPα declines in the model of Alzheimer’s disease, leading to excessive microglia mediated synapse elimination as well as impaired cognitive function.

The scale of the fractures can vary, making the seismic velocity and anisotropy substantially scale dependent. Two mechanisms of the scale-dependent phenomenon may be considered: scattering and wave-induced fluid flow. In this study, we measure the scale-dependent velocity and anisotropy effects through laboratory experiments on porous and non-porous artificial rocks containing aligned fractures. This allows us to isolate the effects of these two mechanisms for the first time, yielding some insights into the scale-dependent phenomenon. For short-wavelength waves, scattering dominates with less wave-induced fluid flow effects. For intermediate- and long-wavelength waves, the P-wave is strongly scale dependent mainly due to wave-induced fluid flow mechanism, and the slow shear-wave is also strongly scale dependent but due to both scattering and wave-induced fluid flow. However, the fast shear-wave is almost scale independent. Moreover, a multi-scale equivalent medium theory can model the P-wave propagation accurately.

In face of the everlasting battle toward COVID-19 and the rapid evolution of SARS-CoV-2, no specific and effective drugs for treating this disease have been reported until today. Angiotensin-converting enzyme 2 (ACE2), a receptor of SARS-CoV-2, mediates the virus infection by binding to spike protein. Although ACE2 is expressed in the lung, kidney, and intestine, its expressing levels are rather low, especially in the lung. Considering the great infectivity of COVID-19, we speculate that SARS-CoV-2 may depend on other routes to facilitate its infection. Here, we first discover an interaction between host cell receptor CD147 and SARS-CoV-2 spike protein. The loss of CD147 or blocking CD147 in Vero E6 and BEAS-2B cell lines by anti-CD147 antibody, Meplazumab, inhibits SARS-CoV-2 amplification. Expression of human CD147 allows virus entry into non-susceptible BHK-21 cells, which can be neutralized by CD147 extracellular fragment. Viral loads are detectable in the lungs of human CD147 (hCD147) mice infected with SARS-CoV-2, but not in those of virus-infected wild type mice. Interestingly, virions are observed in lymphocytes of lung tissue from a COVID-19 patient. Human T cells with a property of ACE2 natural deficiency can be infected with SARS-CoV-2 pseudovirus in a dose-dependent manner, which is specifically inhibited by Meplazumab. Furthermore, CD147 mediates virus entering host cells by endocytosis. Together, our study reveals a novel virus entry route, CD147-spike protein, which provides an important target for developing specific and effective drug against COVID-19.

Utilization of the torsional wave signal has been reported in medical testing and engineering detection. A torsional wave is a shear-wave train whose vibrating direction (polarization) forms a torsional motion. However, there is a lack of understanding of its characteristics and potential for subsurface investigations. Here, compressional (P), shear (S), and torsional (T) transducers are constructed using different piezoelectric-transformer (PZT) chips in the laboratory, and the characteristics of the torsional (T) wave propagating in isotropic and fracture-induced anisotropic media are analyzed in line with conventional P- and S-waves, covering waveform, amplitude, arrival time, and velocity at different propagating angles. The results indicate that the velocity and anisotropy values measured using the T transducer are almost the same as the SH-wave. For propagation in isotropic and anisotropic media, the wavefield generated by the T transducer is very simple with no mode conversions and source-generated interferences, which is similar to the SH-wavefield, while the SV-wavefield comprises a series of complex events from mode conversions and interferences. For propagating in fluid-saturated fractured rocks, the torsional wavefield also comprises a fast and slow wave train, which is referred to as torsional wave splitting, and is similar to the shear-wave splitting from S transducers. Furthermore, the anisotropy parameters measured using P, S, and T transducers show a good correlation with the fracture density, and the P-wave anisotropy is higher in air-saturated rocks than water-saturated rocks, while the shear-wave anisotropy measured by the S and T transducers is sensitive to fracture density. Like the P transducer, the T transducer is polarization-independent, or directional invariant. Therefore, utilizing the T transducer may simplify the field logistics during 3D shear-wave surveys. However, field torsional wave generation is a major issue that needs further study.