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RNA-mediated chromatin silencing is central to genome regulation in many organisms. However, how nascent non-coding transcripts regulate chromatin is poorly understood. Here, through analysis of Arabidopsis FLC , we show that resolution of a nascent-transcript-induced R-loop promotes chromatin silencing. Stabilization of an antisense-induced R-loop at the 3′ end of FLC enables an RNA binding protein FCA, with its direct partner FY/WDR33 and other 3′-end processing factors, to polyadenylate the nascent antisense transcript. This clears the R-loop and recruits the chromatin modifiers demethylating H3K4me1. FCA immunoprecipitates with components of the m 6 A writer complex, and m 6 A modification affects dynamics of FCA nuclear condensates, and promotes FLC chromatin silencing. This mechanism also targets other loci in the Arabidopsis genome, and consistent with this fca and fy are hypersensitive to a DNA damage-inducing drug. These results show how modulation of R-loop stability by co-transcriptional RNA processing can trigger chromatin silencing. Nascent non-coding RNA can mediate chromatin silencing, however mechanistically this process is poorly understood. Here the authors show that resolution of an R-loop during 3'-end processing of a plant antisense transcript recruits chromatin modifiers to promote chromatin silencing.

Arabidopsis REVERSION-TO-ETHYLENE SENSITIVITY1 (RTE1) represses ethylene hormone responses by promoting ethylene receptor ETHYLENE RESPONSE1 (ETR1) signaling, which negatively regulates ethylene responses. To investigate the regulation of RTE1, we performed a genetic screening for mutations that suppress ethylene insensitivity conferred by RTE1 overexpression in Arabidopsis. We isolated HYPER RECOMBINATION1 (HPR1), which is required for RTE1 overexpressor (RTE1ox) ethylene insensitivity at the seedling but not adult stage. HPR1 is a component of the THO complex, which, with other proteins, forms the TRanscription EXport (TREX) complex. In yeast, Drosophila, and humans, the THO/TREX complex is involved in transcription elongation and nucleocytoplasmic RNA export, but its role in plants is to be fully determined. We investigated how HPR1 is involved in RTE1ox ethylene insensitivity in Arabidopsis. The hpr1-5 mutation may affect nucleocytoplasmic mRNA export, as revealed by in vivo hybridization of fluorescein-labeled oligo(dT)45 with unidentified mRNA in the nucleus. The hpr1-5 mutation reduced the total and nuclear RTE1 transcript levels to a similar extent, and RTE1 transcript reduction rate was not affected by hpr1-5 with cordycepin treatment, which prematurely terminates transcription. The defect in the THO-interacting TEX1 protein of TREX but not the mRNA export factor SAC3B also reduced the total and nuclear RTE1 levels. SERINE-ARGININE-RICH (SR) proteins are involved mRNA splicing, and we found that SR protein SR33 co-localized with HPR1 in nuclear speckles, which agreed with the association of human TREX with the splicing machinery. We reveal a role for HPR1 in RTE1 expression during transcription elongation and less likely during export. Gene expression involved in ethylene signaling suppression was not reduced by the hpr1-5 mutation, which indicates selectivity of HPR1 for RTE1 expression affecting the consequent ethylene response. Thus, components of the THO/TREX complex appear to have specific roles in the transcription or export of selected genes.

Quantitative transcriptional control is essential for physiological and developmental processes in many organisms. Transcriptional output is influenced by cotranscriptional processes interconnected to chromatin regulation, but how the functions of different cotranscriptional regulators are integrated is poorly understood. The Arabidopsis floral repressor locus FLOWERING LOCUS C (FLC) is cotranscriptionally repressed by alternative processing of the antisense transcript COOLAIR. Proximal 3′-end processing of COOLAIR resolves a cotranscriptionally formed R-loop, and this process physically links to a histone-modifying complex FLD/SDG26/LD. This induces a chromatin environment locally that determines low transcription initiation and a slow elongation rate to both sense and antisense strands. Here, we show that ARGONAUTE1 (AGO1) genetically functions in this cotranscriptional repression mechanism. AGO1 associates with COOLAIR and influences COOLAIR splicing dynamics to promote proximal COOLAIR, R-loop resolution, and chromatin silencing. Proteomic analyses revealed physical associations between AGO1, subunits of RNA Polymerase II (Pol II), the splicing-related proteins—the spliceosome NineTeen Complex (NTC) and related proteins (NTR)—and the THO/TREX complex. We connect these activities by demonstrating that the THO/TREX complex activates FLC expression acting antagonistically to AGO1 in COOLAIR processing. Together these data reveal that antagonistic cotranscriptional regulation through AGO1 or THO/TREX influences COOLAIR processing to deliver a local chromatin environment that determines FLC transcriptional output. The involvement of these conserved cotranscriptional regulators suggests similar mechanisms may underpin quantitative transcriptional regulation generally.

INTRODUCTION: While the highly efficient interaction models of digital-intelligent technology bring convenience, they have also triggered widespread social alienation and psychological burnout, highlighting the necessity for critical examination and reshaping of digital spaces. OBJECTIVES: This paper aims to employ Lefebvre's theory of the production of space as a framework to systematically analyze the emotional healing mechanisms of low-interaction products as a \"restorative micro-space,\" clarifying the intrinsic pathways through which their \"production\" process influences user psychology and emotions. METHODS: The study constructs a chained mediation model encompassing the three dimensions of spatial production (12 design elements), basic psychological needs (autonomy, competence, relatedness), and emotional healing. Through the development of low-interaction product design practices and the establishment of a mainstream calendar application as a reference group, the theoretical model was empirically examined using structural equation modeling (SEM) and multi-group analysis. RESULTS: Empirical results strongly support the theoretical model, indicating that the core advantage of low-interaction products lies in their \"non-performative\" and \"nurturing\" design. These elements effectively restore users' sense of autonomy and relatedness, thereby achieving significant emotional healing effects CONCLUSION: This research provides empirically grounded theoretical guidance for design practices focused on digital mental health, validating the effectiveness and superiority of using the theory of spatial production to guide the construction of a \"restorative micro-space.\"  

Quantitative variation in expression of the Arabidopsis floral repressor FLC influences whether plants overwinter before flowering, or have a rapid cycling habit enabling multiple generations a year. Genetic analysis has identified activators and repressors of FLC expression but how they interact to set expression level is poorly understood. Here, we show that antagonistic functions of the FLC activator FRIGIDA (FRI) and the repressor FCA, at a specific stage of embryo development, determine FLC expression and flowering. FRI antagonizes an FCA-induced proximal polyadenylation to increase FLC expression and delay flowering. Sector analysis shows that FRI activity during the early heart stage of embryo development maximally delays flowering. Opposing functions of cotranscriptional regulators during an early embryonic developmental window thus set FLC expression levels and determine flowering time.

Arabidopsis REVERSION-TO-ETHYLENE SENSITIVITY1 (RTE1) represses ethylene hormone responses by promoting ethylene receptor ETHYLENE RESPONSE1 (ETR1) signaling, which negatively regulates ethylene responses. To investigate the regulation of RTE1, we performed a genetic screening for mutations that suppress ethylene insensitivity conferred by RTE1 overexpression in Arabidopsis. We isolated HYPER RECOMBINATION1 (HPR1), which is required for RTE1 overexpressor (RTE1ox) ethylene insensitivity at the seedling but not adult stage. HPR1 is a component of the THO complex, which, with other proteins, forms the TRanscription EXport (TREX) complex. In yeast, Drosophila, and humans, the THO/TREX complex is involved in transcription elongation and nucleocytoplasmic RNA export, but its role in plants is to be fully determined. We investigated how HPR1 is involved in RTE1ox ethylene insensitivity in Arabidopsis. The hpr1-5 mutation may affect nucleocytoplasmic mRNA export, as revealed by in vivo hybridization of fluorescein-labeled oligo(dT)45 with unidentified mRNA in the nucleus. The hpr1-5 mutation reduced the total and nuclear RTE1 transcript levels to a similar extent, and RTE1 transcript reduction rate was not affected by hpr1-5 with cordycepin treatment, which prematurely terminates transcription. The defect in the THO-interacting TEX1 protein of TREX but not the mRNA export factor SAC3B also reduced the total and nuclear RTE1 levels. SERINE-ARGININE-RICH (SR) proteins are involved mRNA splicing, and we found that SR protein SR33 co-localized with HPR1 in nuclear speckles, which agreed with the association of human TREX with the splicing machinery. We reveal a role for HPR1 in RTE1 expression during transcription elongation and less likely during export. Gene expression involved in ethylene signaling suppression was not reduced by the hpr1-5 mutation, which indicates selectivity of HPR1 for RTE1 expression affecting the consequent ethylene response. Thus, components of the THO/TREX complex appear to have specific roles in the transcription or export of selected genes.

The essence of art design is the process of emotional interaction with humans. In this paper, emotions are classified using multimodal fusion features, and abstract fusion features are obtained by sampling the multimodal matching tensor using average pooling. The matching fusion matrix in the tensor operator is used to convert from two-modal to multi-modal matching. Virtual interaction model in art design controls the design objectives, and the optimized virtual world is constructed by using virtual reality technology, so as to build an immersive art design model. Finally, a study was conducted to examine the impact of the use of emotion perception and interaction technology in art product design with students from the School of Design and Art, University of G. The results show that the liking degree of three-color matching in the cognitive experiment test is 0.307, which is higher than the liking degree of two-color matching of 0.223, indicating that overall three-color matching samples are more popular in art design. This study provides effective evaluation and guidance for designing art products that are emotional and innovative.

Background Obesity is a risk factor for asthma, but heterogeneity remains. The relationship between different metabolic obesity phenotypes and asthma has not been previously studied. Methods We used data from the National Health and Nutrition Examination Survey (NHANES), including a total of 50,544 participants. We compared the nationally weighted prevalence of asthma across different obesity statuses, metabolic conditions, and their combined states, and estimated asthma risk. Patients with asthma were identified through questionnaire data. Weighted chi-square tests were used to compare asthma prevalence between groups. Multivariable weighted logistic regression analyses were used to estimate asthma risk. Results In the total population, obesity (OR = 1.32, P  < 0.001) and hypertension (OR = 1.19, P  < 0.001) were risk factors for asthma, while hyperglycemia (OR = 1.09, P  = 0.062) and dyslipidemia (OR = 0.96, P  = 0.254) was not significantly associated with higher asthma risk. Compared to metabolically healthy non-obese individuals, metabolically healthy obese individuals had a higher risk of asthma. Furthermore, when obesity was combined with two or more metabolic risk factors, the risk of asthma was higher by 39% (OR = 1.39, P  < 0.001). This effect was more pronounced in females(OR = 1.52 in females vs OR = 1.22 in females, both P  < 0.05). Further analysis revealed that, in the total population, obesity combined with isolated hypertension was significantly associated with higher risk of asthma (OR = 1.49, P  = 0.001). Moreover, among females, obesity combined with isolated hyperglycemia, hypertension or dyslipidemia was all associated with higher risk of asthma. Conclusion Both obesity and metabolic abnormalities independently increase asthma risk, and their combined effects pose an even greater threat, particularly among females. These findings highlight the importance of simultaneously addressing both obesity and metabolic abnormalities to reduce asthma risk.

The large-scale fabrication and patterning of artificial perceptual systems are vital for the development of bionic systems. Traditional patterning processes are often constrained by the use of mask versions, which are not only expensive but also challenging to produce on a large scale. Inkjet printing technology with maskless patterning capability is well suited for current demands for large-scale preparation and patterning. However, the application of printing techniques is typically confined to the production of simple devices or the preparation of patterned active layers within more complex devices. In this study, we successfully fabricated fully inkjet-printed indium gallium zinc oxide (IGZO) memristor arrays to mimic artificial nociceptor (pain receptors). By integrating a layer of silver into the all metal-oxide indium tin oxide (ITO)/IGZO/ITO memristor, we achieved stable threshold switching characteristics, a large current switching ratio of 10 5 , excellent switching durability of 10 4 cycles scans, and excellent spatial uniformity. We investigated the Ag-based conductive filament conduction mode and mimic LIF characteristics (leaky, integrate and fire), demonstrating the potential of the memristor as an artificial neuron. Lastly, we successfully implemented artificial nociceptor, including “threshold fire”, “relaxation”, “non-adaptation”, and “sensitization”, leveraging the stable threshold switching properties of the device. Our work demonstrates the significant potential of inkjet printing technology in the realization of bionic systems.

Context: The clusters Abell 3017 and Abell 3016 are located within a large-scale filament. A prominent X-ray bridge has been detected connecting the two clusters and a potential galaxy group between them. Aims: The aim of this work is to investigate the existence of a radio bridge in the filament between Abell 3017 and Abell 3016, to explore other diffuse radio structures within this system, and to investigate the origins of these diffuse radio emission. Methods: We analyzed MeerKAT L-band data to study the morphology and spectra of the diffuse radio structures in Abell 3016-Abell 3017. X-ray imaging and spectral analysis were carried out with archival Chandra and XMM-Newton data. Additionally, correlations between radio (\\(I_R\\)) and X-ray surface brightness (\\(I_X\\)) were generated to explore the connections between thermal and non-thermal components in the diffuse radio emission. Results: We detected a faint radio bridge with an average surface brightness of \\(\\sim 0.1~\\mu\\rm Jy~arcsec^{-2}\\) at 1280 MHz using MeerKAT. It connects Abell 3017 with a potential galaxy group and extends towards Abell 3016, aligning with the X-ray bridge. A high X-ray temperature of \\(7.09 \\pm 0.54\\) keV detected in the bridge region suggests an interaction between Abell 3017 and the group. In Abell 3017, we identified two distinct components of diffuse radio emission: a radio mini-halo and an outer radio halo with a northern extension (N-extension hereafter). The radio surface brightness profile of Abell 3017 shows a steep inner component consistent with other mini-halos, and a faint outer component likely linked to an infalling subcluster. The \\(I_{\\rm R}-I_{\\rm X}\\) diagram indicates superlinear and sublinear correlations for the mini-halo and N-extension, respectively.