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Small interfering RNA (siRNA) is an effective therapeutic to regulate the expression of target genes in vitro and in vivo. Constructing a siRNA delivery system with high serum stability, especially responsive to endogenous stimuli, remains technically challenging. Herein we develop anti-degradation Y-shaped backbone-rigidified triangular DNA bricks with sticky ends (sticky-YTDBs) and tile them onto a siRNA-packaged gold nanoparticle in a programmed fashion, forming a multi-functional three-dimensional (3D) DNA shell. After aptamers are arranged on the exterior surface, a biocompatible siRNA-encapsulated core/shell nanoparticle, siRNA/Ap-CS, is achieved. SiRNAs are internally encapsulated in a 3D DNA shell and are thus protected from enzymatic degradation by the outermost layer of YTDB. The siRNAs can be released by endogenous miRNA and execute gene silencing within tumor cells, causing cell apoptosis higher than Lipo3000/siRNA formulation. In vivo treatment shows that tumor growth is completely (100%) inhibited, demonstrating unique opportunities for next-generation anticancer-drug carriers for targeted cancer therapies. Small interfering RNA (siRNA) is used to regulate gene expression for therapeutic purposes, but the design of stable and efficient siRNA delivery systems is challenging. Here, the authors develop a siRNA-encapsulated and aptamer-incorporated core/shell nanoparticle for controlled siRNA delivery, with high stability, tumor-specific targeting and long circulation time.

With the development of the times, the financial industry is constantly changing. Blockchain technology continues to play an important role in supply chain finance. The security of the financial industry is very important. Blockchain technology can better protect the security of the financial industry, but there are also some risks. We have made a detailed investigation and research on the risk assessment and analysis of blockchain technology in supply chain finance. The research is as follows: (1) the risks under blockchain technology are introduced in detail, and the risks in many aspects are detailed. It is convenient for the public to do a good job in risk control in the use of blockchain finance. (2) The algorithm of the blockchain is used for mining. In finance, the security of privacy is the most important. The differential privacy in the algorithm and the Bc-ppkCa algorithm makes the financial privacy under the blockchain better. It is beneficial for the public to use blockchain technology more efficiently and safely. (3) The financial industry under the blockchain is very complex. Let us take the financial situation under industrial enterprises as an example. Investigate the company’s supply chain financial situation data, and compare the advantages of supply chain finance and the company’s financial situation. It also evaluates the risks generated by supply chain finance and makes corresponding analysis.

Optical control of structural and electronic properties of Weyl semimetals allows development of switchable and dissipationless topological devices at the ultrafast scale. An unexpected orbital-selective photoexcitation in type-II Weyl material WTe 2 is reported under linearly polarized light (LPL), inducing striking transitions among several topologically-distinct phases mediated by effective electron-phonon couplings. The symmetry features of atomic orbitals comprising the Weyl bands result in asymmetric electronic transitions near the Weyl points, and in turn a switchable interlayer shear motion with respect to linear light polarization, when a near-infrared laser pulse is applied. Consequently, not only annihilation of Weyl quasiparticle pairs, but also increasing separation of Weyl points can be achieved, complementing existing experimental observations. In this work, we provide a new perspective on manipulating the Weyl node singularity and coherent control of electron and lattice quantum dynamics simultaneously. Photoexcitation in Weyl semimetals is recently reported to induce topological phase transitions useful for ultrafast switching devices. Here, the authors predict that the symmetry of the atomic orbitals comprising the Weyl bands in response to linear light polarization allows for not only annihilation but also separation of Weyl quasiparticles.

Activatable theranostic systems combining precise diagnosis and robust immune activation have significant potential in cancer treatment. Herein, we develop a versatile nanoplatform integrating hypoxia-activatable molecular imaging with effective photoimmunotherapy for cancer treatment. Our molecular probe features turn-on near-infrared-II (NIR-II) fluorescence and photoacoustic signals in hypoxic tumor environments. It also induces hypoxia-triggered photodynamic and photothermal effects, promoting immunogenic cell death and activating the STING pathway, engaging both innate and adaptive immunity. The molecular probe is formulated with a vascular disrupting agent to amplify the hypoxia-responsive phototheranostic properties, on which M1-like macrophage membrane is camouflaged to shield against premature release while conferring cancer-targeting affinity. The activatable NIR-II fluorescence and photoacoustic imaging enable precise tumor delineation, while the enhanced phototherapy activates tumor-specific cytotoxic T cells, impeding both primary and distant tumor progression and providing protective immunity against rechallenge in 4T1 tumor-bearing female mice. This work advances activatable theranostic protocols for image-guided immunotherapy. Here the authors report the development and characterization of a nanoplatform that integrates hypoxia-activatable molecular imaging and photo-immunotherapy for cancer diagnosis and immunotherapy.

Researchers frequently utilize the method of optical initial structure (MOIS) of Czerny–Turner (C–T) spectrometers for aberration-correction studies based on the coma-free equation. While effective, this method has limitations: small numerical apertures at slits (0.05–0.07) hinder weak signal detection; V or W-shaped variations in Airy disk across wavelengths; optical resolution depends on the radius of the collimating lens may not match detector resolution; and sequence patterns based on the spot diagrams cannot simulate the full width at half maximum (FWHM) under discrete sampling. To address these issues, using ray tracing and imaging equations, three criteria are proposed: luminous flux and aberration balance (LFAB), Airy disk variation at imaging points (ADVI), and optical-detector resolution matching (ORDR). A verification system with a 500–750 nm wavelength range and 0.4 nm resolution was designed. Results show that designing spectrometers based on these criteria increases the slit’s numerical aperture to 0.11 while controlling aberrations. After optimization, the tangential Airy disk size decreased by 28% with variations within 3 μm. Discrete sampling indicates FWHM pixel errors remain within 1/2 pixel of the theoretical value, and FWHM is at least 2.5 pixels, satisfying stricter sampling requirements beyond Nyquist. Optimization only involves adjusting the image plane by 0.017 mm axially, 0.879 mm off-axis, and 0.48° eccentricity. This research strengthens spectrometer design theory and improves practical applications.

The origin of charge density waves (CDWs) in TiSe 2 has long been debated, mainly due to the difficulties in identifying the timescales of the excitonic pairing and electron–phonon coupling (EPC). Without a time-resolved and microscopic mechanism, one has to assume simultaneous appearance of CDW and periodic lattice distortions (PLD). Here, we accomplish a complete separation of ultrafast exciton and PLD dynamics and unravel their interplay in our real-time time-dependent density functional theory simulations. We find that laser pulses knock off the exciton order and induce a homogeneous bonding–antibonding transition in the initial 20 fs, then the weakened electronic order triggers ionic movements antiparallel to the original PLD. The EPC comes into play after the initial 20 fs, and the two processes mutually amplify each other leading to a complete inversion of CDW ordering. The self-amplified dynamics reproduces the evolution of band structures in agreement with photoemission experiments. Hence we resolve the key processes in the initial dynamics of CDWs that help elucidate the underlying mechanism. The physical origins of charge density waves in 1T-TiSe2 and their response to ultrafast excitation have long been a topic of theoretical and experimental debate. Here the authors present an ab initio theory that successfully captures the observed dynamics of charge density wave formation.

Background Inflammatory reactions induced by spinal cord injury (SCI) are essential for recovery after SCI. Atractylenolide III (ATL‐III) is a natural monomeric herbal bioactive compound that is mainly derived in Atractylodes macrocephala Koidz and has anti‐inflammatory and neuroprotective effects. Objective Here, we speculated that ATL‐III may ameliorate SCI by modulating microglial/macrophage polarization. In the present research, we focused on investigating the role of ATL‐III on SCI in rats and explored the potential mechanism. Methods The protective and anti‐inflammatory effects of ATL‐III on neuronal cells were examined in a rat SCI model and lipopolysaccharide (LPS)‐stimulated BV2 microglial line. The spinal cord lesion area, myelin integrity, and surviving neurons were assessed by specific staining. Locomotor function was evaluated by the Basso, Beattie, and Bresnahan (BBB) scale, grid walk test, and footprint test. The activation and polarization of microglia/macrophages were assessed by immunohistofluorescence and flow cytometry. The expression of corresponding inflammatory factors from M1/M2 and the activation of relevant signaling pathways were assessed by Western blotting. Results ATL‐III effectively improved histological and functional recovery in SCI rats. Furthermore, ATL‐III promoted the transformation of M1 into M2 and attenuated the activation of microglia/macrophages, further suppressing the expression of corresponding inflammatory mediators. This effect may be partly mediated by inhibition of neuroinflammation through the NF‐κB, JNK MAPK, p38 MAPK, and Akt pathways. Conclusion This study reveals a novel effect of ATL‐III in the regulation of microglial/macrophage polarization and provides initial evidence that ATL‐III has potential therapeutic benefits in SCI rats. ATL‐III has potential therapeutic benefits in promoting histological and functional repair in rats after SCI. This effect may be partly mediated by inhibiting neuroinflammation induced by microglial polarization through the NF‐κB, JNK MAPK, p38 MAPK, and Akt pathways.

Public open spaces are important venues for children’s participation in outdoor activities and social life. This study performs a comparative and qualitative review of the tools that can be used to audit the environments of children-focused public open spaces. The analysis reviews 25 studies involving 11 tools for comparison. The results reveal that (1) the tools were developed in different fields; (2) the tools use two data resources, field investigation and geographic databases; (3) the tool dimensions are diverse, as are the number of items covered, and are generally related to four categories: surrounding environment and accessibility, activity and perceived safety, children’s sports and play opportunities, and aesthetic and comfort of the environment; (4) the reliability of most tools has been verified, with some validity still to be confirmed; (5) there are differences in tool users, settings, and aims. Among the tools, the CPAT and the EAPRS are the most comprehensive. Comparative analysis of the tools provides a reference for studies on children-focused public open spaces and for the development and improvement of corresponding tools in the future.

BackgroundsImmunotherapy is less effective in patients with epidermal growth factor receptor (EGFR) mutant non-small-cell lung cancer (NSCLC). Lower programmed cell death-ligand 1 (PD-L1) expression and tumor mutation burden (TMB) are reported to be the underlying mechanism. Being another important factor to affect the efficacy of immunotherapy, tumor microenvironment (TME) characteristics of this subgroup of NSCLC are not comprehensively understood up to date. Hence, we initiated this study to describe the specific TME of EGFR-mutant lung adenocarcinoma (LUAD) from cellular compositional and functional perspectives to better understand the immune landscape of this most common subtype of NSCLC.MethodsWe used single-cell transcriptome sequencing and multiplex immunohistochemistry to investigate the immune microenvironment of EGFR-mutant and EGFR wild-type LUADs and determined the efficacy of immunotherapy. We analyzed single cells from nine treatment-naïve samples and compared them to three post-immunotherapy samples previously reported from single cell perspective using bioinformatics methods.ResultsWe found that EGFR-mutant malignant epithelial cells had similar characteristics to the epithelial cells in non-responders. EGFR-mutant LUAD lacked CD8+ tissue-resident memory (TRM) cells, which could promote tertiary lymphoid structure generation by secreting CXCL13. In addition, other cell types, including tumor-associated macrophages and cancer-associated fibroblasts, which are capable of recruiting, retaining, and expanding CD8+ TRM cells in the TME, were also deficient in EGFR-mutant LUAD. Furthermore, EGFR-mutant LUAD had significantly less crosstalk between T cells and other cell types via programmed cell death-1 (PD-1) and PD-L1 or other immune checkpoints compared with EGFR wild-type LUAD.ConclusionsOur findings provide a comprehensive understanding of the immune landscape of EGFR-mutant LUAD at the single-cell level. Based on the results, many cellular components might have negative impact on the specific TME of EGFR-mutant LUAD through influencing CD8+ TRM. Lack of CD8+ TRM might be a key factor responsible for the suppressive TME of EGFR-mutant LUAD.

The ultimate goal of phototherapy based on nanoparticles, such as photothermal therapy (PTT) which generates heat and photodynamic therapy (PDT) which not only generates reactive oxygen species (ROS) but also induces a variety of anti-tumor immunity, is to kill tumors. In addition, due to strong efficacy in clinical treatment with minimal invasion and negligible side effects, it has received extensive attention and research in recent years. In this paper, the generations of nanomaterials in PTT and PDT are described separately. In clinical application, according to the different combination pathway of nanoparticles, it can be used to treat different diseases such as tumors, melanoma, rheumatoid and so on. In this paper, the mechanism of pathological treatment is described in detail in terms of inducing apoptosis of cancer cells by ROS produced by PDT, immunogenic cell death to provoke the maturation of dendritic cells, which in turn activate production of CD4+ T cells, CD8+T cells and memory T cells, as well as inhibiting heat shock protein (HSPs), STAT3 signal pathway and so on.