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Background Virtual Reality (VR) technology has become an essential preclinical training tool in the field of oral and maxillofacial surgery education. This study aimed to evaluate the effectiveness of VR simulation system for penetrating facial wound suture skill training. Methods 26 stomatology undergraduates were recruited to attend the preclinical training using the virtual training system. Students were asked to learn and practice the program for 5 consecutive days and complete the final test. The scores of each test were recorded and assessed to evaluate the effects of the training. Finally, a self-designed questionnaire was distributed to the students, and qualitative interviews were conducted to assess the applicability and acceptability of this virtual training system. Results Five steps of wound debridement and suture including “History and Physical Examination”, “Wound Inspection”, “Pre-operation preparation”, “Suturing”, “Post-operative instructions” as well as the total score were shown with statistically significant differences among the five training results. With the increased number of practices, the performance gradually improved. Most students agreed that this system helped them familiarize with the clinical procedure due to its simulation of the real clinical environment (88.46%) and increased the availability of time and space for practice without limitation (100%). Totally speaking, Most students (92.31%) agreed that the VR simulation was interesting and useful. Conclusions The case-based VR simulation software for penetrating facial wound suture skill training positively influences medical students’ clinical reasoning, operative skills, and self-confidence. It provides an efficient strategy for conserving resources while providing quality education for medical students.

Multi-rotor unmanned aerial vehicle (UAV) is a new chemical application tool for tall stalk tropical crop Areca catechu , which could improve deposit performance, reduce operator healthy risk, and increase spraying efficiency. In this work, a spraying experiment was carried out in two A. catechu fields with two leaf area index (LAI) values, and different operational parameters were set. Spray deposit quality, spray drift, and ground loss were studied and evaluated. The results showed that the larger the LAI of A. catechu , the lesser the coverage of the chemical deposition. The maximum coverage could reach 4.28% and the minimum 0.33%. At a flight speed of 1.5 m/s, sprayed droplets had the best penetration and worst ground loss. The overall deposition effect was poor when the flight altitudes were greater than 11.09 m and the flight speed was over 2.5 m/s. Comparing flight speed of 2.5 to 1.5 m/s, the overall distance of 90% of the total drift increased to double under the same operating parameters. This study presents reference data for UAV chemical application in A. catechu protection.

This study aimed to describe the clinical features of different types of traumatic temporomandibular joint (TMJ) ankylosis. Seventy-one patients with 102 ankylosed joints were retrospectively reviewed and categorized into four groups according to the grades of severity: type I, non-bony ankylosis of the joint with almost-normal joint space; type II, lateral bony ankylosis marked by a normal joint space that coexists with a radiolucent line; type III, complete bony ankylosis of the joint characterized by only a radiolucent line; and type IV, extensive bony ankylosis without any radiolucent line. The period of ankylosis, maximal mouth opening (MMO), rate of complications, and histopathological changes were compared among groups. Intergroup comparison showed significant differences in the clinical features of MMO and the incidence of complications (p < 0.05). Younger trauma patients tended to develop more severe types of ankylosis than older patients. Additionally, long post-trauma periods were related to the development of severe ankylosis. MMO was highly negatively correlated with the severity of ankylosis. Significant differences were noted among the four types of ankylosis. Younger trauma patients with long post-trauma periods tended to develop more severe TMJ ankylosis, experience more complications, and face more challenges in treatment than older patients.

Unmanned aerial vehicles (UAVs) have been widely used in plant protection, and the mechanism of droplet deposition drift while spraying with the 3WQF120-12 produced by Quanfeng Aviation, a representative model of single-rotor plant protection UAVs in China, still requires more research. This study used a combination of computational fluid dynamics (CFD) and wind tunnel experiments to analyze the droplet deposition drift pattern of the 3WQF120-12 single-rotor plant protection UAV. The CFD modeling of the nozzle was confirmed to be feasible using wind tunnel experiments. Pearson correlation analysis was performed between experimental and simulated values, and multiple correlation coefficients reached above 0.89, which is a robust correlation. In this study, CFD simulations were performed to simulate the drift of UAV spray droplets under the rotor wind field and the combined effect of front and side winds. The deposition of droplets at different heights was simulated. The UAV’s spraying conditions at different flight speeds, side wind magnitudes, and spraying heights were evaluated. According to the CFD simulation results of the 3WQF120-12 plant protection UAV, the recommended flight height is 1–3 m, the recommended flight speed is below 3 m/s, and the recommended ambient wind speed is within 3 m/s. The simulation results were verified by the field test, and the trend of the field experimental data and CFD simulation results are qualitatively consistent to verify the reasonableness and feasibility of the simulation’s data. The simulated results were similar to the curves and spray area of the field test results at operating heights of 1.5 m and 3.5 m.

Hydrogen sulfide, the third gaseous transmitter, is one of the main causes of halitosis in the oral cavity. It is generally considered as playing a deleterious role in many oral diseases including oral cancer. However, the regulatory mechanisms involved in the effects of hydrogen sulfide on oral cancer growth remain largely unknown. In the present study, we investigated the underlying mechanisms through CCK-8 assay, EdU incorporation, real-time PCR, western blot and pathway blockade assays. Our results showed that hydrogen sulfide promoted oral cancer cell proliferation through activation of the COX2, AKT and ERK1/2 pathways in a dose-dependent manner. Blocking any of the three above pathways inhibited hydrogen sulfide-induced oral cancer cell proliferation. Meanwhile, blockade of COX2 by niflumic acid downregulated NaHS-induced p-ERK and p-AKT expression. Inactivation of the AKT pathway by GSK690693 significantly decreased NaHS-induced p-ERK1/2 expression, and inhibition of the ERK1/2 pathway by U0126 markedly increased NaHS-induced p-AKT expression. Either the AKT or ERK1/2 inhibitor did not significantly alter the COX2 expression level. Our data revealed, for the first time, that hydrogen sulfide promotes oral cancer cell proliferation through activation of the COX2/AKT/ERK1/2 axis, suggesting new potential targets to eliminate the effect of hydrogen sulfide on the development of oral cancer.

Ruminants are a diverse group of mammals that includes families containing well-known taxa such as deer, cows, and goats. However, their evolutionary relationships have been contentious, as have the origins of their distinctive digestive systems and headgear, including antlers and horns (see the Perspective by Ker and Yang). To understand the relationships among ruminants, L. Chen et al. sequenced 44 species representing 6 families and performed a phylogenetic analysis. From this analysis, they were able to resolve the phylogeny of many genera and document incomplete lineage sorting among major clades. Interestingly, they found evidence for large population reductions among many taxa starting at approximately 100,000 years ago, coinciding with the migration of humans out of Africa. Examining the bony appendages on the head—the so-called headgear—Wang et al. describe specific evolutionary changes in the ruminants and identify selection on cancer-related genes that may function in antler development in deer. Finally, Lin et al. take a close look at the reindeer genome and identify the genetic basis of adaptations that allow reindeer to survive in the harsh conditions of the Arctic. Science , this issue p. eaav6202 , p. eaav6335 , p. eaav6312 ; see also p. 1130 The genetics of distinctive reindeer characteristics are identified from their genome. The reindeer is an Arctic species that exhibits distinctive biological characteristics, for which the underlying genetic basis remains largely unknown. We compared the genomes of reindeer against those of other ruminants and nonruminant mammals to reveal the genetic basis of light arrhythmicity, high vitamin D metabolic efficiency, the antler growth trait of females, and docility. We validate that two reindeer vitamin D metabolic genes ( CYP27B1 and POR ) show signs of positive selection and exhibit higher catalytic activity than those of other ruminants. A mutation upstream of the reindeer CCND1 gene endows an extra functional binding motif of the androgen receptor and thereby may result in female antlers. Furthermore, a mutation (proline-1172→threonine) in reindeer PER2 results in loss of binding ability with CRY1, which may explain circadian arrhythmicity in reindeer.

Background Tumor-associated macrophages (TAMs) play a critical role in modulating the tumor microenvironment and promote tumor metastases. Our studies have demonstrated that ginsenoside Rh2 (G-Rh2), a monomeric compound extracted from ginseng, is a promising anti-tumor agent in lung cancer cells. However, it remains unclear whetherG-Rh2 can modulate the differentiation of TAMs and its interaction with tumor microenvironment. In this study, we investigated how G-Rh2 regulates the phenotype of macrophages and affects the migration of non-small cell lung cancer (NSCLC) cells. Methods Murine macrophage-like RAW264.7 cells and human THP-1 monocyte were differentiated into M1 and M2 subsets of macrophages with different cytokines combination, which were further identified by flow cytometry with specific biomarkers. M2 macrophages were sorted out to co-culture with NSCLC cell lines, A549 and H1299. Wound healing assay was performed to examine the cell migration. Expression levels of matrix metalloproteinases 2 and 9 (MMP-2, − 9) and vascular endothelial growth factor-C (VEGF-C) were measured by RT-qPCR and western blot, and the release of VEGF in the supernatant was measured by a VEGF ELISA kit. Finally, modulation of TAMs phenotype and VEGF expression by G-Rh2 was examined in vivo. Results We demonstrated that M2 subset of macrophages alternatively differentiated from RAW264.7 or THP-1cells promote migration of NSCLC cells. Further examinations revealed that NSCLC significantly increased the release of VEGF to the media and elevated the expression levels of VEGF at mRNA and protein levels after being co-cultured with M2 macrophages. Similar alterations in MMP-2 and MMP-9 were observed in NSCLC after being co-cultured. Of note,G-Rh2 had a potential to effectively convert M2 phenotype to M1 subset of macrophages. Importantly, G-Rh2 had a preference to decrease the expression levels of VEGF, MMP2, and MMP9 in co-cultured lung cancer cells, over than those in lung cancer cells without co-culturing. Consistently, G-Rh2 reduced M2 macrophage marker CD206 and VEGF expression levels in vivo. Conclusions All of these results suggested that M2 subset macrophages drive lung cancer cells with more aggressive phenotypes. G-Rh2 has a potential to convert TAMs from M2 subset to M1 in the microenvironment and prevents lung cancer cell migration, suggesting the therapeutic effects of G-Rh2onlung cancer.

Necrotizing enterocolitis (NEC) is a devastating disease observed in premature infants, characterized by intestinal ischemia and inflammation. Hypoxia‐inducible factor‐1 alpha (HIF‐1α), a master regulator of the cellular response to hypoxia and ischemia, plays a critical role in NEC pathogenesis. However, the precise mechanisms by which HIF‐1α influences the intestines in NEC remain poorly understood. Herein, we aimed to explore the role of HIF‐1α in NEC using a transgenic mouse model. We induced NEC in neonatal mice from postnatal day 5 to 9, and various parameters, including intestinal injury, oxidative stress, inflammatory responses, intestinal epithelial cell (IEC) proliferation, and apoptosis, were assessed. The results confirmed that the absence of intestinal epithelial HIF‐1α increased the susceptibility of mice to NEC‐induced intestinal injury, as evidenced by increased oxidative stress, inflammatory responses, apoptosis, and inhibition of proliferation. Additionally, we observed an upregulation of the Toll‐like receptor 4 (TLR4)/nuclear factor kappa B (NF‐κB) signaling pathway specifically in the intestines of mice lacking HIF‐1α in IECs (HIF‐1α ΔIEC ) with NEC. These findings provide crucial insights into the role of HIF‐1α in regulating intestinal oxidative stress and inflammation to maintain intestinal homeostasis, highlighting its association with the TLR4–NF‐κB signaling pathway. Furthermore, these insights might lead to the identification of novel therapeutic targets for the treatment of NEC.

Relieving the stress or strain associated with volume change is highly desirable for high‐performance SiOx anodes in terms of stable solid electrolyte interphase (SEI)‐film growth. Herein, a Si‐valence gradient is optimized in SiOx composites to circumvent the large volume strain accompanied by lithium insertion/extraction. SiOx@C annealed at 850°C has a gentle Si‐valence gradient along the radial direction and excellent electrochemical performances, delivering a high capacity of 506.9 mAh g−1 at 1.0 A g−1 with a high Coulombic efficiency of ~99.8% over 400 cycles. Combined with the theoretical prediction, the obtained results indicate that the gentle Si‐valence gradient in SiOx@C is useful for suppressing plastic deformation and maintaining the inner connection integrity within the SiOx@C particle. Moreover, a gentle Si‐valence gradient is expected to form a stress gradient and affect the distribution of dangling bonds, resulting in local stress relief during the lithiation/delithiation process and enhanced Li‐ion kinetic diffusion. Furthermore, the lowest interfacial stress variation ensures a stable SEI film at the interface and consequently increases cycling stability. Therefore, rational design of a Si‐valence gradient in SiOx can provide further insights into achieving high‐performance SiOx anodes with large‐scale production. It is highly desirable to develop a novel internal‐stress‐relief strategy to suppress SiOx expansion via design and optimization of Si valence in SiOx composites for high‐energy‐density lithium‐ion batteries. In our work, enhanced electrochemical performances of SiOx are obtained via optimizing the Si‐valence gradient from the viewpoint of stress evolution upon cycling.

Abstract Background: Biliary tract carcinomas (BTCs) are relatively rare but lethal primary malignant tumors derived from the biliary tract system. The burden of BTCs varies according to sex, age, region, and country, but limited attention has been paid to the burden of BTCs. We sought to explore the up-to-date data from the Global Burden of Disease Study (GBD) and expand findings by accessing the demographic features of BTC disease burden. Methods: Using the latest data from the GBD 2021, we evaluated and analyzed the distributions and patterns of BTC disease burden in various age groups, sexes, regions, and countries. Results: The number of incident cases, deaths, and disability-adjusted life-years (DALYs) tended to increase and peaked at 216,770 (95% uncertainty interval [UI]: 181,890–245,240), 171,960 (95% UI: 142,350–194,240), and 3,732,100 (95% UI: 3,102,900–4,317,000) person-years, respectively, in 2021. However, the average global age-standardized rates (ASRs) of incident cases, deaths, and DALYs shrunk by −11.46% (95% UI: −21.91 to 3.35%), −24.09% (95% UI: −33.19 to 16.88%), and −26.25% (95% UI: −35.53 to 18.36%), respectively, from 1990 to 2021. Meanwhile, the male/female ratio (male per 100 female) of incidence, deaths, and DALYs changed from 76.40, 75.41, and 74.72 to 86.89, 79.11, and 82.29, respectively. In 2021, the highest number of incident cases, deaths, and DALYs occurred in East Asia. The top three highest incidences, deaths, and DALYs were observed in China, India, and Japan, and the highest ASRs were observed in Chile in 2021. Analysis of the Human Development Index along with disease burden estimates of BTCs also suggests that the burden of the disease is related to the level of comprehensive development of the society. Conclusion: This study provided a comprehensive comparison of differences in the burden of disease across populations and over time, and further presented evidence concerning the formulation of prevention and control policies and etiologic studies for BTCs and proposed logical hypotheses to investigate.