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Pyroptosis, a type of programmed cell death illuminated by inflammasomes and active caspases, is implicated in post-stroke inflammation. Our previous study showed that lncRNA taurine upregulated gene 1 ( Tug1 ) sponging miR-145a-5p modulated microglial activation after oxygen-glucose deprivation (OGD). However, the role and mechanism of Tug1 on post-stroke pyroptosis is not fully clear. Photo-thrombosis stroke mice and OGD-treated BV-2 microglia were established respectively. Tug1 knockdown or overexpression was achieved by intraventricular infusion of AAV-sh Tug1 in vivo, or transfection of si Tug1 and pcDNA3.1- Tug1 in vitro. Neurological function and infarction volume were evaluated. Meanwhile, pyroptosis-associated proteins (IL-1β, IL-18, NLRP3, ASC, cleaved-caspase-1, and GSDMD-N), TLR4, and p-p65/p65 as well as Tug1 and miR-145a-5p were detected 24 h after photo-thrombosis or 4 h after OGD by qRT-PCR, western blot, and ELISA. The correlation between Tug1 /miR-145a-5p/ Tlr4 axis and pyroptosis was explored by dual-luciferase reporter assay and functional gain-and-loss experiments. Photo-thrombosis or OGD caused neural injury and upregulated pyroptosis-associated proteins, Tug1 , TLR4, and p-p65 as well as downregulated miR-145a-5p, which was prevented by Tug1 knockdown in vivo and in vitro. Tlr4 gene, putatively binding with miR-145a-5p by bioinformatics analysis, was found to be a direct target of miR-145a-5p with negative interactions. Furthermore, miR-145a-5p inhibitor abolished the inhibitive effects of si Tug1 on TLR4 and p-p65 as well as pyroptosis-associated proteins, whereas miR-145a-5p mimics abrogated the enhanced effects of pcDNA3.1- Tug1 on that, suggesting an involvement of Tug1 /miR-145a-5p/ Tlr4 axis on pyroptosis. Tug1 contributes NLRP3 inflammasome-dependent pyroptosis through miR-145a-5p/ Tlr4 axis post-stroke, providing a promising therapeutic strategy against inflammatory injury.

Background Root-knot nematode is one of the most significant diseases of vegetable crops in the world. Biological control with microbial antagonists has been emerged as a promising and eco-friendly treatment to control pathogens. The aim of this study was to screen and identify novel biocontrol agents against root-knot nematode, Meloidogyne incognita . Results A total of 890 fungal isolates were obtained from rhizosphere soil of different crops and screened by nematicidal activity assays. Snef1910 strain showed high virulence against second stage juveniles (J2s) of M. incognita and identified as Trichoderma citrinoviride by morphology analysis and biomolecular assay. Furthermore, T. citrinoviride Snef1910 significantly inhibited egg hatching with the hatching inhibition percentages of 90.27, 77.50, and 67.06% at 48, 72, and 96 h after the treatment, respectively. The results of pot experiment showed that the metabolites of T. citrinoviride Snef1910 significantly decreased the number of root galls, J2s, and nematode egg masses and J2s population density in soil and significantly promoted the growth of tomato plants. In the field experiment, the biocontrol application showed that the control efficacy of T. citrinoviride Snef1910 against root-knot nematode was more than 50%. Meanwhile, T. citrinoviride Snef1910 increased the tomato plant biomass. Conclusions T. citrinoviride strain Snef1910 could be used as a potential biological control agent against root-knot nematode, M. incognita .

Backgrounds Research has demonstrated that elevated serum total bilirubin (STB) levels have a beneficial impact on various diseases, particularly metabolic syndrome. This study aims to investigate the association between STB levels and serum testosterone (STT) in order to determine if bilirubin plays a protective role in relation to testosterone deficiency (TD) risk. Methods In this study, a total of 6,526 eligible male participants aged 20 years or older were analyzed, all of whom took part in the National Health and Nutrition Examination Survey (NHANES) conducted between 2011 and 2016. To investigate the relationship between STB and STT levels, we employed weighted multivariate regression models along with restricted cubic splines (RCS). Additionally, a subgroup analysis was conducted to explore the heterogeneity of this relationship across different subpopulations. Results Among the participants, 1,832 individuals (28.07%) were identified as having testosterone deficiency (TD), defined as an STT level below 300 ng/dL. A significant positive correlation between STB and STT levels was observed in both crude and adjusted models (all P  < 0.0001). The association between STB and STT levels was found to be statistically significant up to a threshold of 17.1 µmol/L, after which it became statistically insignificant( P for non-linearity = 0.0035). Weighted logistic regression analysis indicated that a 1 µmol/L increase in STB was associated with a 4% decrease in the likelihood of TD (odds ratio = 0.96, P  < 0.0001). Subgroup analysis showed that the inverse relationship was limited to individuals aged 60 and over, non-smokers/drinkers, and obese individuals. Conclusion STB within the physiological range(17.1 µmol/L) was positively associated with STT in adult males. The potential protective role of bilirubin regarding testosterone levels merits further exploration.

MEMS-based LIDAR has the advantages of fast-scanning, simple structure, small volume, and light weight, which make it a hot topic for 3D imaging. In order to overcome the limited scanning angle of the current MEMS mirrors, a large angle MEMS-based optical scanning system was designed using telephoto structure. In this design an f-θ lens group is used for flat field scanning and a symmetrical cemented lens group is used for angle expansion. The principle of this system is discussed in detail and an optical design is implemented by ZEMAX software. The designed system realized a scanning angle as large as 57° and is in excellent linear relation with the tilt angle of the MEMS mirror. At the target distance of 100 m, the maximum RMS radius of the light spot is only 7 cm. This optical scanning system shows a significant application potentiality for 3D imaging LIDAR.

With an increasing concern of the food security, more and more winemakers choose bioprotection as an alternative of SO 2 in winemaking process as a practice of natural wine producing. In order to insight a way to produce reliable natural wine, three wines were produced in vintage 2021, in the Republic Moldova. This study provides two comparisons of physicochemical property and sensory analysis: (1) Comparison between commercial yeast and wild yeast, two wines followed a conventional fermentation technique, but different yeasts were used: Saccharomyces cerevisiae (wine A), wild yeast (wine); (2) Comparison between bioprotection and SO 2 usage: Wine A and Wine C (which were inoculated two non- Saccharomyces : Torulaspora delbrueckii and Metschnikowia pulcherrima before alcohol fermentation). As a result of this comparative study, it is found that the commercial yeast is more capable of converting sugar in the alcoholic fermentation, but after malolatic fermentation (MLF) the alcohol levels of each are almost same. From physicochemical poit of view, the bioprotectors obviously modified the volatile acidity, total polyphenol index (TPI), phenolic, anthoyanin and ethyl acetate. From sensory perspective, the smell intensity of bioprotection wine is higher and with more fruity aroma.

Background We previously performed targeted sequencing of autism risk genes in probands from the Autism Clinical and Genetic Resources in China (ACGC) (phase I). Here, we expand this analysis to a larger cohort of patients (ACGC phase II) to better understand the prevalence, inheritance, and genotype–phenotype correlations of likely gene-disrupting (LGD) mutations for autism candidate genes originally identified in cohorts of European descent. Methods We sequenced 187 autism candidate genes in an additional 784 probands and 85 genes in 599 probands using single-molecule molecular inversion probes. We tested the inheritance of potentially pathogenic mutations, performed a meta-analysis of phase I and phase II data and combined our results with existing exome sequence data to investigate the phenotypes of carrier parents and patients with multiple hits in different autism risk genes. Results We validated recurrent, LGD, de novo mutations (DNMs) in 13 genes. We identified a potential novel risk gene ( ZNF292 ), one novel gene with recurrent LGD DNMs ( RALGAPB ), as well as genes associated with macrocephaly ( GIGYF2 and WDFY3 ). We identified the transmission of private LGD mutations in genes predominantly associated with DNMs and showed that parental carriers tended to share milder autism-related phenotypes. Patients that carried DNMs in two or more candidate genes show more severe phenotypes. Conclusions We identify new risk genes and transmission of deleterious mutations in genes primarily associated with DNMs. The fact that parental carriers show milder phenotypes and patients with multiple hits are more severe supports a multifactorial model of risk.

This study delves into the sensory intricacies of 20 Fetească Neagră (FN) wines originating from the 2019 and 2020 vintages. By conducting a comprehensive analysis, we explore the dynamic relationship between vintage, aroma, and taste attributes. Notably, discernible differences in aroma and taste profiles between the two years come to light. The descriptors of Forest fruit and oak aromas emerge as pivotal indicators of vintage variations, offering reliable tools for discerning aroma disparities. Significant taste differences, excluding bitter and alcohol, are evident in both vintages. Interestingly, descriptors including prune, cherry, black pepper, plant, smoke, bitter, and alcohol demonstrate consistent qualities across both years. These findings underscore their potential as stable markers in FN wines’ sensory profiles. The study sheds light on the multifaceted influences, from oak treatment to natural factors, shaping wine’s sensory characteristics. This research not only advances our understanding of sensory attributes but also aids producers in informed decision-making to achieve desired flavor profiles, preserving the distinct essence of FN wines across diverse vintages.

Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused great harm to global public health, resulting in a large number of infections among the population. However, the epidemiology of coronavirus has not been fully understood, especially the mechanism of aerosol transmission. Many respiratory viruses can spread via contact and droplet transmission, but increasing epidemiological data have shown that viral aerosol is an essential transmission route of coronavirus and influenza virus due to its ability to spread rapidly and high infectiousness. Aerosols have the characteristics of small particle size, long-time suspension and long-distance transmission, and easy access to the deep respiratory tract, leading to a high infection risk and posing a great threat to public health. In this review, the characteristics of viral aerosol generation, transmission, and infection as well as the current advances in the aerosol transmission of zoonotic coronavirus and influenza virus are summarized. The aim of the review is to strengthen the understanding of viral aerosol transmission and provide a scientific basis for the prevention and control of these diseases.

For the conventional vineyard, reducing the pesticide usage is the most important issue for converting to organic vineyard. In order to study the effect of pesticide usage on grape yeast, an investigation were designed in vintage 2020, ten vineyards (include two organic vineyards) with different treatment frequency index (TFI) were chosen, and the yeast population counted by two method: cultivate method and the direct epifluorescence technique (DEFT). The results show, the grape yeast from Moldova exist the viable but non-culturable (VBNC) state. In organic vineyards, less proportion yeast reach the VBNC, however the opposite conclusion draw from conventional vineyards. High TFI with high level VBNC yeast population, inversely, less culturable yeast. No matter in which yeast state, the treatment of powdery mildew is the most influential factor on yeast population.

Republic Moldova is a country with long history of winemaking. Understanding the microorganism on the grape surface is very important to the winemaking process, and it's also a national strategy of development of the wine industry. In this study, twenty seven samples from three regions and three vintages in Republic of Moldova were studied. The conventional microbiological methods combine with molecular methods (PCR-DGGE) have been used for study the quantity and the quality of microbes. The result show that the yeast population on the berries are variable in different vintages, and in the climatic factors, the Cool nigt index (CI) affect the yeast most. From the identification result, A.Pullulans and R.glutins are two culture which are easy to be found on the Moldova grapes. The autochthonous S.cerevisiae also been identified, but it shows a different results from different vintages.