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Different land-use types have different carbon sequestration capacities, and changes in land-use type directly cause changes in carbon sequestration capacity. To understand the change trend of carbon sequestration capacity, it is necessary to quantify the specific quantity of the impact of land use change on carbon sequestration capacity to indirectly estimate the change in carbon sequestration capacity by monitoring the change in land use type. Based on the analysis of the changing trends of land use type data and ecological carbon sequestration data in the research area, this study establishes an impact model (IM) of land use change on ecological carbon sequestration capacity, quantifying the specific quantity of the impact of land use change on ecological carbon sequestration capacity from two aspects: the impact of land use type transition in and the impact of land use type transition out. Through verification of the accuracy of the model estimation, it is expected that the impact of land use change on carbon sequestration capacity in Sichuan mountainous areas in 2025 and 2030 will be -20754 tons and -30837 tons, respectively, and the total ecological carbon sinks will be 132 and 133 million tons, respectively. Based on the trend analysis of the total carbon sequestration and the IM estimation data, the impact of land use change on ecological carbon sequestration capacity tends to stabilize (the impact will fluctuate around -31156 tons), and the trend of a significant increase in total carbon sequestration is not obvious. The IM quantifies the specific quantity of the influence of land-use type change on carbon sequestration capacity and can dynamically analyze the external factors that cause the change in carbon sequestration capacity, which is of great significance for monitoring the change law of carbon sequestration capacity.

OsbZIP23 is a member of the basic leucine zipper (bZIP) transcription factor family in rice (Oryza sativa). Expression of OsbZIP23 is strongly induced by a wide spectrum of stresses, including drought, salt, abscisic acid (ABA), and polyethylene glycol treatments, while other stress-responsive genes of this family are slightly induced only by one or two of the stresses. Transactivation assay in yeast demonstrated that OsbZIP23 functions as a transcriptional activator, and the sequences at the N terminus (amino acids 1-59) and a region close to the C terminus (amino acids 210-240) are required for the transactivation activity. Transient expression of OsbZIP23-green fluorescent protein in onion (Allium cepa) cells revealed a nuclear localization of the protein. Transgenic rice overexpressing OsbZIP23 showed significantly improved tolerance to drought and high-salinity stresses and sensitivity to ABA. On the other hand, a null mutant of this gene showed significantly decreased sensitivity to a high concentration of ABA and decreased tolerance to high-salinity and drought stress, and this phenotype can be complemented by transforming the OsbZIP23 back into the mutant. GeneChip and real-time polymerase chain reaction analyses revealed that hundreds of genes were up- or down-regulated in the rice plants overexpressing OsbZIP23. More than half of these genes have been annotated or evidenced for their diverse functions in stress response or tolerance. In addition, more than 30 genes that are possible OsbZIP23-specific target genes were identified based on the comparison of the expression profiles in the overexpressor and the mutant of OsbZIP23. Collectively, these results indicate that OsbZIP23 functions as a transcriptional regulator that can regulate the expression of a wide spectrum of stress-related genes in response to abiotic stresses through an ABA-dependent regulation pathway. We propose that OsbZIP23 is a major player of the bZIP family in rice for conferring ABA-dependent drought and salinity tolerance and has high potential usefulness in genetic improvement of stress tolerance.

The lack of spectrum resources restricts the development of wireless communication applications. In order to solve the problems of low spectrum utilization and channel congestion caused by the static division of spectrum resource, this paper proposes an optimal linear weighted cooperative spectrum sensing for clustered-based cognitive radio networks. In this scheme, different weight values will be assigned for cooperative nodes according to the SNR of cognitive users and the historical sensing accuracy. In addition, the cognitive users can be clustered, and the users with the better channel characteristics will be selected as cluster heads for gathering the local sensing information. Simulation results show that the proposed scheme can obtain better sensing performance, improve the detection probability and reduce the error probability.

The TIFY family is a novel plant-specific gene family involved in the regulation of diverse plant-specific biologic processes, such as development and responses to phytohormones, in Arabidopsis. However, there is limited information about this family in monocot species. This report identifies 20 TIFY genes in rice, the model monocot species. Sequence analysis indicated that rice TIFY proteins have conserved motifs beyond the TIFY domain as was previously shown in Arabidopsis. On the basis of their protein structures, members of the TIFY family can be divided into two groups. Transcript level analysis of OsTIFY genes in tissues and organs revealed different tempo-spatial expression patterns, suggesting that expression and function vary by stage of plant growth and development. Most of the OsTIFY genes were predominantly expressed in leaf. Nine OsTIFY genes were responsive to jasmonic acid and wounding treatments. Interestingly, almost all the OsTIFY genes were responsive to one or more abiotic stresses including drought, salinity, and low temperature. Over-expression of OsTIFY11a, one of the stress-inducible genes, resulted in significantly increased tolerance to salt and dehydration stresses. These results suggest that the OsTIFY family may have important roles in response to abiotic stresses. The data presented in this report provide important clues for further elucidating the functions of the genes in the OsTIFY family.

Background Ovarian cancer (OC) is a common fatal malignant tumor of female reproductive system worldwide. Growing studies have proofed that circular RNAs (circRNAs) engage in the regulation of various types of cancers. However, the underlying biological functions and effect mechanism of circular RNA_LARP4 (circ_LARP4) in OC have not been explored. Methods Quantitative real-time polymerase chain reaction (qRT-PCR) analysis was used to detect the expression of circ_LARP4 in OC cells. The function of circ_LARP4 was measured by cell counting kit-8 (CCK-8), colony formation assay and transwell assay. RNA immunoprecipitation (RIP) assay and luciferase reporter assays assessed the binding correlation between miR-513b-5p and circ_LARP4 (or LARP4). Results The expression of circ_LARP4 in OC cells was much lower than that in human normal ovarian epithelial cells. Overexpressing circ_LARP4 impaired cell proliferation, invasion and migration abilities. Circ_LARP4 worked as a competing endogenous RNA (ceRNA) to sponge miR-513b-5p. Furthermore, LARP4 was indirectly modulated by circ_LARP4 as the downstream target of miR-513b-5p, as well as the host gene of circ_LARP4. Conclusion Circ_LARP4 could hamper cell proliferation and migration by sponging miR-513b-5p to regulate the expression of LARP4. This research may provide some referential value to OC treatment.

During the accelerated transition toward greener and more decarbonized development, urban regeneration initiatives have emerged as an important policy instrument for enhancing the synergistic effect of pollution and carbon reduction (SEPCR). Using panel data for 282 prefecture-level-and-above cities from 2006 to 2022, this study exploits the Ecological Restoration and Urban Repair (ERUR) pilot as an exogenous policy shock and applies a multi-period DID framework to identify its impact on SEPCR. The results show that the ERUR policy significantly enhances SEPCR by approximately 1.9%, and the findings remain robust across multiple robustness checks, including parallel trend tests, stacked DID, and double machine learning. Heterogeneity analyses reveal that policy effects differ across cities with varying geographical locations, resource endowments, industrial characteristics, and spatial forms. Mechanism analyses suggest that the improvement in SEPCR is primarily driven by economies of scale, industrial structure upgrading, and energy intensity improvement. This study extends the quantitative assessment framework of SEPCR and provides robust empirical evidence on how urban regeneration initiatives can be systematically harnessed to enhance urban SEPCR performance, offering important academic insights and policy-relevant implications for promoting green transformation, urban sustainable development, and ecological civilization construction.

Objective This systematic review and meta-analysis investigated whether the use of azithromycin during labour or caesarean section reduces the incidence of sepsis and infection among mothers and newborns. Data sources We independently searched the PubMed, Web of Science, Cochrane Library and EMBASE databases for relevant studies published before February, 2024. Methods We included RCTs that evaluated the effect of prenatal oral or intravenous azithromycin or placebo on intrapartum or postpartum infection incidence. We included studies evaluating women who had vaginal births as well as caesarean sections. Studies reporting maternal and neonatal infections were included in the current analysis. Review Manager 5.4 was used to analyse 6 randomized clinical trials involving 44,448 mothers and 44,820 newborns. The risk of bias of each included study was assessed using the criteria outlined in the Cochrane Handbook for Systematic Reviews of Interventions.Primary outcomes included the incidence of maternal sepsis and all-cause mortality and neonatal sepsis and all-cause mortality; secondary outcomes included maternal (endometritis, wound and surgical site infections, chorioamnionitis, and urinary tract infections) and neonatal outcomes (infections of the eyes, ears and skin). A random-effects model was used to test for overall effects and heterogeneity. Results The pooled odds ratios (ORs) were as follows: 0.65 for maternal sepsis (95% CI, 0.55–0.77; I 2 , 0%; P  < .00001); 0.62 for endometritis (95% CI, 0.52–0.74; I 2 , 2%; P  < .00001); and 0.43 for maternal wound or surgical site infection (95% CI, 0.24–0.78; P  < .005); however, there was great heterogeneity among the studies ( I 2 , 75%). The pooled OR for pyelonephritis and urinary tract infections was 0.3 (95% CI, 0.17–0.52; I 2 , 0%; P  < .0001), and that for neonatal skin infections was 0.48 (95% CI, 0.35–0.65; I 2 , 0%, P  < .00001). There was no significant difference in maternal all-cause mortality or incidence of chorioamnionitis between the two groups. No significant differences were observed in the incidence of neonatal sepsis or suspected sepsis, all-cause mortality, or infections of the eyes or ears. Conclusion In this meta-analysis, azithromycin use during labour reduced the incidence of maternal sepsis, endometritis, incisional infections and urinary tract infections but did not reduce the incidence of neonatal-associated infections, except for neonatal skin infections. These findings indicate that azithromycin may be potentially beneficial for maternal postpartum infections, but its effect on neonatal prognosis remains unclear. Azithromycin should be used antenatally only if the clinical indication is clear and the potential benefits outweigh the harms.

Background Successful neoadjuvant chemotherapy (NACT) could improve the surgical resection rate and radical curability of patients with cervical cancer, but only a subset of patients benefits. Therefore, identifying predictive biomarkers are urgently needed. The aim of this study was to evaluate the predictive value of CD34 and Bcl-2 in the NACT effectiveness of cervical cancer. Methods Sixty-seven patients with locally advanced cervical cancer (FIGO stages IB3, IIA2 or IIB) were classified into two groups based on effective ( n  = 48) and ineffective ( n  = 19) response to NACT. Immunohistochemistry was employed to identify CD34 and Bcl-2 expression before and after NACT. We analyzed the associations between the pre-NACT expression of these two biomarkers and the response of NACT. The expression of these two biomarkers before and after NACT was also assessed and compared. Results More patients were CD34 positive expression before NACT in effective group compared to ineffective group ( p  = 0.005). However, no statistically significant difference in Bcl-2 expression before NACT were found between two groups ( p  = 0.084). In NACT effective group, the expression of both CD34 and Bcl-2 after NACT are down-regulated ( p  < 0.001 and p  < 0.001, respectively), while there are no statistical differences between the pre- and post-NACT expression of CD34 and Bcl-2 in NACT ineffective group ( p  = 0.453 and p  = 0.317, respectively). Conclusion The positive CD34 expression before NACT may serve as a predictive biomarker for NACT of cervical cancer, but the pre-NACT expression of Bcl-2 is not an independent predictor. The down-regulated expression of these two indicators after NACT may indicate effective NACT.

Based on the framework of our previous work [H.L. Lai et al., Phys. Rev. E, 94, 023106 (2016)], we continue to study the effects of Knudsen number on two-dimensional Rayleigh–Taylor (RT) instability in compressible fluid via the discrete Boltzmann method. It is found that the Knudsen number effects strongly inhibit the RT instability but always enormously strengthen both the global hydrodynamic non-equilibrium (HNE) and thermodynamic non-equilibrium (TNE) effects. Moreover, when Knudsen number increases, the Kelvin–Helmholtz instability induced by the development of the RT instability is difficult to sufficiently develop in the later stage. Different from the traditional computational fluid dynamics, the discrete Boltzmann method further presents a wealth of non-equilibrium information. Specifically, the two-dimensional TNE quantities demonstrate that, far from the disturbance interface, the value of TNE strength is basically zero; the TNE effects are mainly concentrated on both sides of the interface, which is closely related to the gradient of macroscopic quantities. The global TNE first decreases then increases with evolution. The relevant physical mechanisms are analyzed and discussed.

The outermost surfaces of plants are covered with an epicuticular wax layer that provides a primary waterproof barrier and protection against different environmental stresses. Glossy 1 (GL1) is one of the reported genes controlling wax synthesis. This study analyzed GL1-homologous genes in Oryza sativa and characterized the key members of this family involved in wax synthesis and stress resistance. Sequence analysis revealed 11 homologous genes of GL1 in rice, designated OsGL1-1 to OsGL1-11. OsGL1-1, -2 and -3 are closely related to GL1. OsGL1-4, -5, -6, and -7 are closely related to Arabidopsis CER1 that is involved in cuticular wax biosynthesis. OsGL1-8, -9, -10 and -11 are closely related to SUR2 encoding a putative sterol desaturase also involved in epicuticular wax biosynthesis. These genes showed variable expression levels in different tissues and organs of rice, and most of them were induced by abiotic stresses. Compared to the wild type, the OsGL1-2-over-expression rice exhibited more wax crystallization and a thicker epicuticular layer; while the mutant of this gene showed less wax crystallization and a thinner cuticular layer. Chlorophyll leaching experiment suggested that the cuticular permeability was decreased and increased in the over-expression lines and the mutant, respectively. Quantification analysis of wax composition by GC-MS revealed a significant reduction of total cuticular wax in the mutant and increase of total cuticular wax in the over-expression plants. Compared to the over-expression and wild type plants, the osgl1-2 mutant was more sensitive to drought stress at reproductive stage, suggesting an important role of this gene in drought resistance.