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Background Applying traditional fluorescence navigation technologies in hepatocellular carcinoma is severely restricted by high false-positive rates, variable tumor differentiation, and unstable fluorescence performance. Results In this study, a green, economical and safe nanomedicine formulation technology was developed to construct carrier-free indocyanine green nanoparticles (nanoICG) with a small uniform size and better fluorescent properties without any molecular structure changes compared to the ICG molecule. Subsequently, nanoICG dispersed into lipiodol via a super-stable homogeneous intermixed formulation technology (SHIFT&nanoICG) for transhepatic arterial embolization combined with fluorescent laparoscopic hepatectomy to eliminate the existing shortcomings. A 52-year-old liver cancer patient was recruited for the clinical trial of SHIFT&nanoICG. We demonstrate that SHIFT&nanoICG could accurately identify and mark the lesion with excellent stability, embolism, optical imaging performance, and higher tumor-to-normal tissue ratio, especially in the detection of the microsatellite lesions (0.4 × 0.3 cm), which could not be detected by preoperative imaging, to realize a complete resection of hepatocellular carcinoma under fluorescence laparoscopy in a shorter period (within 2 h) and with less intraoperative blood loss (50 mL). Conclusions This simple and effective strategy integrates the diagnosis and treatment of hepatocellular carcinoma, and thus, it has great potential in various clinical applications.

Corneal neovascularization (CNV) is one of the common blinding factors worldwide, leading to reduced vision or even blindness. However, current treatments such as surgical intervention and anti-VEGF agent therapy still have some shortcomings or evoke some adverse effects. Recently, SU6668, an inhibitor targeting angiogenic tyrosine kinases, has demonstrated growth inhibition of neovascularization. But the hydrophobicity and low ocular bioavailability limit its application in cornea. Hereby, we proposed the preparation of SU6668 pure nanoparticles (NanoSU6668; size ~135 nm) using a super-stable pure-nanomedicine formulation technology (SPFT), which possessed uniform particle size and excellent aqueous dispersion at 1 mg/mL. Furthermore, mesenchymal stem cell membrane vesicle (MSCm) was coated on the surface of NanoSU6668, and then conjugated with TAT cell penetrating peptide, preparing multifunctional TAT-MSCm@NanoSU6668 (T-MNS). The T-MNS at a concentration of 200 µg/mL was treated for CNV via eye drops, and accumulated in blood vessels with a high targeting performance, resulting in elimination of blood vessels and recovery of cornea transparency after 4 days of treatment. Meanwhile, drug safety test confirmed that T-MNS did not cause any damage to cornea, retina and other eye tissues. In conclusion, the T-MNS eye drop had the potential to treat CNV effectively and safely in a low dosing frequency, which broke new ground for CNV theranostics.

Corneal neovascularization (CNV) is a debilitating ocular surface disease that severely compromises visual function and carries a significant risk of vision loss. Despite its clinical impact, the development of effective and safe pharmacological treatments for CNV remains an unmet medical need. The pathogenesis of CNV is largely driven by inflammation and excessive oxidative stress. In this study, we introduce a novel nanotherapeutic strategy utilizing vanadium carbide quantum dots (V2C QDs) with intrinsic nanozyme properties, co‐encapsulated with a plasmid encoding interleukin‐10 (IL‐10) within a biomimetic metal‐organic framework (MOF) for the treatment of CNV. To enhance targeting and biocompatibility, the nanoparticles (NPs) are further coated with mesenchymal stem cell (MSC)‐derived cell membrane vesicles (CMVs), yielding the final nanomedicine designated as MOF‐V2C‐Plasmid@CMVs (MVPC). In vitro studies demonstrate that MVPC NPs effectively scavenge reactive oxygen species (ROS) induced by tert‐butyl hydroperoxide (tBOOH), mitigating oxidative stress. Moreover, the successful delivery and expression of the IL‐10 plasmid in RAW264.7 cells result in elevated IL‐10 secretion, showcasing robust anti‐inflammatory activity. The CMV coating facilitates targeted delivery, enabling the efficient accumulation of MVPC NPs in the CNV region following topical administration via eye drops. In vivo experiments in CNV model rats reveal that MVPC nanotherapeutics significantly suppress neovascularization without inducing adverse effects. Collectively, this study provides proof of concept for a multifunctional nanotherapeutic platform targeting CNV, offering a promising and clinically translatable approach for the treatment of this challenging ocular disease. An anti‐inflammatory and antioxidant metal‐organic framework (MOF) nanoplatform was prepared via one‐pot self‐assembly. In vitro and in vivo studies demonstrated that the prepared MVPC nanoparticles can effectively scavenge overproduced reactive oxygen species at diseased sites, enhance IL‐10 secretion, and suppress VEGFA expression, offering a novel strategy for safe and effective therapy of corneal neovascularization.

Retinal neovascularization (RNV) drives dual pathological cascades: structural destruction characterized by intraretinal/vitreous hemorrhages and tractional retinal detachment, alongside functional decline marked by progressive neurodegeneration and irreversible vision loss. Current clinical interventions for RNV face critical limitations in targeting specificity and therapeutic durability. To address this, we engineer magnesium acetyl taurate/thalidomide co‐assembled nanoparticles (MT NPs) via Super‐stable Pure Nanomedicine Formulation Technology (SPFT), constructing solvent‐free, coordination‐driven nanostructures with dual‐drug loading. The MT NPs are further coated with transferrin‐modified cell membrane vesicles (Tfm) to form targeted nanocomposites (Tfm@MT NPs). In oxygen‐induced retinopathy (OIR) mouse models, Tfm@MT NPs demonstrated: (1) specific targeting to pathological vascular endothelia and retinal ganglion cells (RGCs) via transferrin receptor‐mediated uptake, (2) sustained drug release exceeding 10 days, and (3) potent therapeutic effects in restoring visual functions. This study establishes a safe and effective targeted nanotherapeutic strategy for RNV, with significant translational potential for retinopathy treatment. Magnesium acetyl taurate and thalidomide co‐assembled nanoparticles (MT NPs) are prepared via Super‐stable Pure Nanomedicine Formulation Technology (SPFT), and the NPs are further coated with transferrin‐modified cell membrane vesicles to construct Tfm@MT nanomedicine for oxygen‐induced retinopathy treatment.

In fiber reinforced composites (FRP), silicon carbide fiber is indispensable. Its mechanical properties determine the performance of ceramic matrix composites. Therefore, the tensile and fracture behavior of silicon carbide fiber monofilaments should be studied in depth. In order to make the test results of fiber properties more accurate, this paper takes SiC fiber monofilament as the research object, carries out tensile tests at different deflection angles, and carries out WEIBULL statistical analysis and comparison of the data. The microscopic morphology of the tensile fracture of each single fiber was observed by SEM, and the change rule of the mechanical properties and fracture behavior of the fiber with the deflection angle was summarized, and whether the slight deflection affected the test results was explored. The results showed that: (1) The mechanical properties of SiC fiber monofilament decreased gradually with the increase of deflection angle, and the extent of decline gradually increased. (2) With the increase of deflection angle, the proportion of stepped fracture gradually increases, and that of flat fracture gradually decreases.

Ceramic matrix composites have excellent properties such as high temperature resistance and oxidation resistance, and have good application prospects in the hot end parts of engines. In ceramic matrix composites, the microstructure composition of silicon carbide fibers determines their macroscopic properties. Therefore, this article takes silicon carbide fiber as the research object, summarizes the existing SiC fibers structure, composition, and morphology characterization methods, compares the development and advantages and disadvantages of XRD, SEM, TEM and other testing equipment analysis, and proposes The problems of sample preparation, surface defects and internal structure changes in the microstructure characterization of silicon carbide fibers are discussed, which provides a reference for the preparation of high-performance SiC fibers and the improvement of their characterization work.

We explored a new artificial intelligence-assisted method to assist junior ultrasonographers in improving the diagnostic performance of uterine fibroids and further compared it with senior ultrasonographers to confirm the effectiveness and feasibility of the artificial intelligence method. In this retrospective study, we collected a total of 3870 ultrasound images from 667 patients with a mean age of 42.45 years ± 6.23 [SD] for those who received a pathologically confirmed diagnosis of uterine fibroids and 570 women with a mean age of 39.24 years ± 5.32 [SD] without uterine lesions from Shunde Hospital of Southern Medical University between 2015 and 2020. The DCNN model was trained and developed on the training dataset (2706 images) and internal validation dataset (676 images). To evaluate the performance of the model on the external validation dataset (488 images), we assessed the diagnostic performance of the DCNN with ultrasonographers possessing different levels of seniority. The DCNN model aided the junior ultrasonographers (Averaged) in diagnosing uterine fibroids with higher accuracy (94.72% vs. 86.63%, P < 0.001), sensitivity (92.82% vs. 83.21%, P = 0.001), specificity (97.05% vs. 90.80%, P = 0.009), positive predictive value (97.45% vs. 91.68%, P = 0.007), and negative predictive value (91.73% vs. 81.61%, P = 0.001) than they achieved alone. Their ability was comparable to that of senior ultrasonographers (Averaged) in terms of accuracy (94.72% vs. 95.24%, P = 0.66), sensitivity (92.82% vs. 93.66%, P = 0.73), specificity (97.05% vs. 97.16%, P = 0.79), positive predictive value (97.45% vs. 97.57%, P = 0.77), and negative predictive value (91.73% vs. 92.63%, P = 0.75). The DCNN-assisted strategy can considerably improve the uterine fibroid diagnosis performance of junior ultrasonographers to make them more comparable to senior ultrasonographers.

Citrus chlorotic dwarf-associated virus (CCDaV) is a Citlodavirus species in the Geminiviridae family that causes tremendous economic loss to the citrus industry in China. Some proteins encoded by geminiviruses are crucial for the interaction between the virus and its host plant. However, the exact functions of CCDaV-encoded proteins such as CCDaV-RepA have not been investigated. This study presents evidence that CCDaV-RepA elicits a hypersensitive response (HR)-like cell death in Nicotiana benthamiana that was accompanied by the production of H 2 O 2 and ion leakage, which suggested that CCDaV-RepA is a potential recognition target for inducing host defense responses. Furthermore, the rolling-circle replication motifs of CCDaV-RepA are associated with triggering HR-like cell death in N. benthamiana . Confocal microscopy and deletion mutagenesis assays showed that CCDaV-RepA was located in the nucleus, while the first eight amino acids (aa) at the N terminus and two regions located between aa residues 122-263 and 220-264 of RepA were not associated with nuclear localization. Tobacco rattle virus-induced gene silencing of the key signaling cascade components revealed that HR-like cell death induced by RepA was inhibited in WRKY1 -silenced N. benthamiana. Moreover, WRKY1 expression was upregulated in RepA-GFP infiltrated Overall, the results suggest that NbWRKY1 positively regulated CCDaV-RepA -induced cell death in N. benthamiana . These findings provide novel information for further research on the interactions between CCDaV and the host plant.

The BESIII experiment has been operating since 2009 and has received several upgrades, to study the τ -charm physics utilizing the BEPCII accelerator. The BEPCII is a double-ring multi-bunch collider with a luminosity of 1 × 10 33 cm - 2 s - 1 optimized at a center-of-mass (cms) energy of 2 × 1.89 GeV. The rich physics topics, long lifetime and the large volume of data present challenges to offline data processing. This paper describes the design and implementation of the offline software system to meet the requirements and challenges of BESIII, including offline software architecture, geometry management, database management, event data model and tag-based analysis.

The complete genomic sequence of a novel citrus tristeza virus (CTV) isolate, CT91-A1, from Orah tangor grafted on Citrus junos cv. Ziyangxiangcheng rootstock in China was determined by transcriptome sequencing. Sequence alignments showed that isolate CT91-A1 shared 83.3 to 95.5% nucleotide sequence identity with extant CTV genotypes at the whole-genome level, with the highest similarity to the S1 genotype. Phylogenetic analysis revealed that CT91-A1 clustered in a unique subclade with the S1 genotype. Isolate CT91-A1 induced severe stem pitting in Mexican lime and C. junos cv. Ziyangxiangcheng and moderate stem pitting in Guanximiyou pummelo and Duncan grapefruit. It was successfully transmitted by Aphis citricidus, and it can potentially cause significant damage to the citrus industry in China.