Explore the vast range of titles available.

This paper presents an adaptive noise reduction method based on Complementary Ensemble Empirical Mode Decomposition (CEEMD) to address the non-stationary characteristics and noise interference present in friction vibration signals from mechanical equipment. and friction testing machine simulation experiments. The performance of CEEMD and Ensemble Empirical Mode Decomposition (EEMD) was compared through MATLAB R2023b simulations and experiments conducted on a friction testing machine. CEEMD achieved a computational efficiency 85.6% higher than that of EEMD and effectively reduced mode aliasing. Among them, the adaptive correlation coefficient screening method performed well in signal reconstruction, and the high correlation (correlation coefficient > 0.8) between the denoised signal and the laboratory noise signal was verified using the multi-scale permutation entropy (MPE) theory, which is of great significance for early diagnosis of mechanical faults, prediction of equipment life and timely maintenance decisions.

p53 plays a critical role in tumor suppression and is the most frequently mutated gene in human cancers. Most p53 mutants (mutp53) are missense mutations and are thus expressed in human cancers. In human cancers that retain wtp53, the wtp53 activities are downregulated through multiple mechanisms. For example, the overexpression of the negative regulators of p53, MDM2/MDMX, can also efficiently destabilize and inactivate wtp53. Therefore, both wtp53 and mutp53 have become promising and intensively explored therapeutic targets for cancer treatment. Current efforts include the development of small molecule compounds to disrupt the interaction between wtp53 and MDM2/MDMX in human cancers expressing wtp53 and to restore wtp53-like activity to p53 mutants in human cancers expressing mutp53. In addition, a synthetic lethality approach has been applied to identify signaling pathways affected by p53 dysfunction, which, when targeted, can lead to cell death. While an intensive search for p53-targeted cancer therapy has produced potential candidates with encouraging preclinical efficacy data, it remains challenging to develop such drugs with good efficacy and safety profiles. A more in-depth understanding of the mechanisms of action of these p53-targeting drugs will help to overcome these challenges.

Sepsis-induced acute lung injury (ALI) is driven by dysregulated innate immunity and mitochondrial dysfunction. Monocyte/macrophage trafficking and polarization critically shape disease trajectory, yet clinically tractable immunometabolic interventions are limited. We hypothesized that 650 nm red-light photobiomodulation (PBM) alleviates septic ALI by reprogramming myeloid responses and preserving mitochondrial function via adiponectin signaling. Septic ALI was induced by cecal ligation and puncture (CLP) in mice. Animals received 650 nm PBM (10 min, every 6 h, three times within 24 h). Survival, lung edema, histology, and serum cytokines were assessed. Lung chemokines/cytokines were profiled by 23-plex Luminex. Immune composition was analyzed by flow cytometry, and CCR2 /CX3CR1 subsets were visualized in CcrRFP-Cx3cr1GFP mice using 3D cryo-fMOST. IHC quantified CX3CR1, CCR2, CD68, CD86, and CD206. Adiponectin was measured in serum/BALF and lung. Pathway relevance was tested by AdipoR1 siRNA. In LPS-stimulated RAW264.7 macrophages, PBM effects on cytokines, ATP, mitochondrial ROS (MitoSOX), membrane potential (JC-1), and MitoTracker fluorescence were evaluated, with/without AdipoR1 knockdown. PBM prolonged survival, reduced lung edema, improved histopathology, and lowered systemic TNF-α, IL-6, IL-1β, and MCP-1. Luminex showed broad suppression of pro-inflammatory mediators (e.g., G-/GM-CSF, IL-1 family, IL-6, IL-12, IL-17A, TNF-α) and chemokines (CCL11, CXCL1, MCP-1/CCL2, CCL3/4/5), with increases in IL-4/IL-10/IL-13. Flow cytometry revealed decreased neutrophils, monocytes, and inflammatory macrophages, alongside restored eosinophils and resident macrophages. Cryo-fMOST and IHC demonstrated reduced CCR2 /CD86 inflammatory cells and enrichment of CX3CR1 /CD206 reparative cells. PBM elevated adiponectin in serum, BALF, and lung; AdipoR1 knockdown abrogated anti-inflammatory effects and myeloid rebalancing. , PBM dose-dependently suppressed LPS-induced TNF-α/IL-6 and IL-1β while increasing IL-10, restored ATP, reduced mitochondrial ROS, and improved membrane potential, that benefits lost with AdipoR1 silencing. Septic ALI modulated by 650 nm PBM was characterized by suppressing CCR2 inflammatory recruitment, enriching CX3CR1 /M2-like macrophages, and preserving mitochondrial function through adiponectin-AdipoR1 signaling. These data position red-light PBM as a mechanistically grounded, non-invasive method for sepsis-associated lung injury.

Background/Aims: ADAMTSs (A disintegrin and metalloprotease domains with thrombospondins motifs) are a family of extracellular proteases that have been related to both oncogenic and tumor-suppressive functions. The aim of the present study was to investigate: 1) the mutation, copy-number alterations, and expression profile of ADAMTSs in colorectal cancer and 2) whether ADAMTSs participate in colorectal cancer (CRC) progression and invasion. Methods: The mutation, copy-number alterations, and expression profile of ADAMTSs in CRC were analyzed in the TCGA cohort using cBioportal. ADAMTS4 expression in tumor tissues and cell lines were determined by immunostaining and real-time quantitative PCR. The role of ADAMTS-4 in CRC progression and the underlying mechanisms were studied by using short hairpin RNA-mediated knockdown of ADAMTS4. The effects of ADAMTS4 in cell proliferation and invasion were determined by clone formation assay and transwell migration assay, respectively. Macrophages were depleted by liposomal clodronate in immune-competent BALB/c mice and tumor growth was analyzed. Results: ADAMTS4 was differentially expressed in CRC and predicted a poor prognosis. Elevated ADAMTS4 expression was closely associated with larger tumor size, enhanced TNM stage, and a poor clinical outcome in patients with CRC. ADAMTS4 knockdown had no inhibitory implications on cell proliferation and invasion in vitro, but significantly attenuated tumor growth in vivo. Mechanistically, we revealed that ADAMTS4 was associated macrophages infiltration and polarization in the tumor microenvironment of CRC. Macrophage depletion largely abolished the promotive effect of ADAMTS4 on tumor growth in the immune competent BALB/c mice. Conclusion: ADAMTS4 seemed to be a promising prognostic indicator in CRC. The novel link between ADAMTS4 and macrophages mirrors the potential regulatory roles of ADAMTSs in the inflammatory microenvironment of cancers.

Endometriosis is defined as a disorder in which the glands and stroma of the endometrium grow and shed periodically outside the uterine cavity. Highly prevalent in women of reproductive age, the most common clinical manifestations are chronic pelvic pain and infertility. The pathogenesis of endometriosis may be multifactorial, including factors of anatomy, immunity, inflammation, hormones (estrogen), oxidative stress, genetics, epigenetics, and environment. There are generally three types of endometriotic disease, namely peritoneal, ovarian, and deep infiltration. For the same patient, there may be a single or multiple types concurrently. The different manifestations of these types suggests that they each have their own etiology. Numerous studies have shown that the evasion of endometrial cells from peritoneal immune surveillance helps establish and maintain peritoneal endometriosis, but the specific mechanism is not well understood. Likewise, the molecular mechanisms of endometriosis-related infertility have not been clearly elucidated. This review attempts to identify the role of peritoneal immunity in peritoneal endometriosis and related infertility, especially in the aspects of molecular mechanisms.

Brake-by-wire (BBW) system is the key system to ensure the safety and stability control of intelligent vehicle chassis. How to evaluate the BBW systems to meet the safety design requirements is one of the important links of system design. This article takes the BBW system as an example to illustrate the basic process of the safety assessment of intelligent vehicle. The fault tree analysis (FTA) method is used to quantitatively evaluate the failure status of the complete loss of the main brake function, and the fault tree analysis process is detailed. The results show that FTA plays an important role in guiding the design of system architecture. With the help of the importance index, we can quickly find out the weaknesses in the system architecture, when the probability of the failure state of the automobile brake-by-wire system cannot meet the ASIL requirements. Furthermore, effective countermeasures can be taken to optimize the design of the system architecture. Finally, the failure probability corresponding to the failure state of the car’s brake-by-wire system is reduced to 7×10 -14 .

The oocyte cumulus complex is mainly composed of an oocyte, the perivitelline space, zona pellucida and numerous granulosa cells. The cumulus granulosa cells (cGCs) provide a particularly important microenvironment for oocyte development, regulating its growth, maturation and meiosis. In this study, we studied the internal structures and cell-to-cell connections of mouse cGCs using focused ion beam scanning electron microscopy (FIB-SEM). We reconstructed three-dimensional models to display characteristic connections between the oocyte and cGCs, and to illustrate various main organelles in cGCs together with their interaction relationship. A special form of cilium identified in granulosa cell was never reported in previous literature.

Quadrature spatial modulation (QSM), where the real and imaginary parts of the transmitted symbol are transmitted separately, was recently proposed to increase the spectral efficiency of spatial modulation. An optimum maximum likelihood (ML) detection was introduced in QSM systems, which leads to high complexity as the exhausting search of all possible transmit antennas. In this paper, a low complexity detection, called signal vector based minimum mean square error (SVMMSE), is proposed for QSM systems. In the proposed SVMMSE detection, we estimate the transmit antenna indices and the transmitted symbol by considering the active transmit antennas in two different scenarios. Simulation results show that the performance of our SVMMSE detection is very close to the ML detection and complexity analysis shows that the computational complexity of the proposed SVMMSE detection is significantly reduced compared with that of the ML detection.

With the globalization of the integrated circuit (IC) design flow of chip fabrication, intellectual property (IP) piracy is becoming the main security threat. While most of the protection methods are dedicated for digital circuits, we are trying to protect radio-frequency (RF) designs. For the first time, we applied the split manufacturing method in RF circuit protection. Three different implementation cases are introduced for security and design overhead tradeoffs, i.e., the removal of the top metal layer, the removal of the top two metal layers and the design obfuscation dedicated to RF circuits. We also developed a quantitative security evaluation method to measure the protection level of RF designs under split manufacturing. Finally, a simple Class AB power amplifier and a more sophisticated Class E power amplifier are used for the demonstration through which we prove that: (1) the removal of top metal layer or the top two metal layers can provide high-level protection for RF circuits with a lower request to domestic foundries; (2) the design obfuscation method provides the highest level of circuit protection, though at the cost of design overhead; and (3) split manufacturing may be more suitable for RF designs than for digital circuits, and it can effectively reduce IP piracy in untrusted off-shore foundries.

In this paper, we investigate an enhanced relay beamforming design for two-way relay networks (TWRN). In order to reduce the computational complexity, we derive a sum of the inverse of the signal-to-noise ratio (SI-SNR) problem equivalent to the objective sum-rate (SR) problem. The SI-SNR problem can be reformulated as a simple optimization problem by using the Cholesky decomposition and Cauchy-Schwarz inequality, and solved by the interior-point method. The numerical results show that the proposed SI-SNR method can not only reduce the computational complexity but also have the same SR performance as that of the conventional works.