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Chili anthracnose is one of the most devastating fungal diseases affecting the quality and yield production of chili. The aim of this review is to summarize the current knowledge concerning the chili anthracnose disease, as well as to explore the use of marker-assisted breeding programs aimed at improving anthracnose disease resistance in this species. This disease is caused by the Colletotrichum species complex, and there have been ongoing screening methods of chili pepper genotypes with resistance to anthracnose in the field, as well as in laboratories. Conventional breeding involves phenotypic selection in the field, and it is more time-consuming compared to molecular breeding. The use of marker-assisted selection (MAS) on the basis of inheritance, the segregation ratio of resistance to susceptibility, and the gene-controlling resistance may contribute to the development of an improved chili variety and speed up the selection process, while also reducing genetic drag in the segregating population. More importantly, by using molecular markers, the linkage groups are determined dominantly and co-dominantly, meaning that the implementation of a reliable method to produce resistant varieties is crucial in future breeding programs. This updated information will offer a supportive direction for chili breeders to develop an anthracnose-resistant chili variety.

Rice, generally classified as a typical glycophyte, often faces abiotic stresses such as excessive drought, high salinity, prolonged submergence, cold, and temperature, which significantly affects growth, development, and ultimately, grain yield. Among these negative impacts of abiotic factors in rice production, salinity stress is a major constraint, followed by drought. There is considerable research on the use of marker-assisted selection (MAS), genome editing techniques, and transgenic studies that have profoundly improved the present-day rice breeders’ toolboxes for developing salt-tolerant varieties. Salinity stresses significantly affect rice plants during seedling and reproductive stages. Hence, greater understanding and manipulation of genetic architecture in developing salt-tolerant rice varieties will significantly impact sustainable rice production. Rice plants’ susceptibility or tolerance to high salinity has been reported to be the result of coordinated actions of multiple stress-responsive quantitative trait loci (QTLs)/genes. This paper reviews recent literature, updating the effects of salinity stress on rice plants and germplasm collections and screening for salinity tolerance by different breeding techniques. Mapping and identification of QTLs salt tolerance genes are illuminated. The present review updates recent breeding for improvement in rice tolerance to salinity stress and how state-of-the-art tools such as MAS or genetic engineering and genome editing techniques, including mutagenesis and conventional breeding techniques, can assist in transferring salt-tolerant QTLs genes into elite rice genotypes, accelerating breeding of salt-resistant rice cultivars.

Andrographis paniculata (Burm.f.) Wall. (Acanthaceae) is revered for its medicinal properties. In vitro culture of medicinal plants has assisted in improving both the quantity and quality of their yield. The current study investigated the effects of different surface sterilization treatments, plant growth regulators (PGRs), and elicitors on culture establishment and axillary shoot multiplication of A. paniculata. Subsequently, the production of andrographolide in the in vitro plantlets was evaluated using high-performance liquid chromatography (HPLC) analysis. The shoot-tip explant was successfully sterilized using 60% commercial bleach for 5 min of immersion with a 90% survival rate and 96.67% aseptic culture. The optimal PGR for shoot growth was 6-benzylaminopurine (BAP) at 17.76 µM, supplemented into Murashige and Skoog (MS) media, producing 23.57 ± 0.48 leaves, 7.33 ± 0.10 shoots, and a 3.06 ± 0.02 cm length of shoots. Subsequently, MS medium supplemented with 5 mg/L chitosan produced 26.07 ± 0.14 leaves, 8.33 ± 0.07 shoots, and a 3.63 ± 0.02 cm length of shoots. The highest andrographolide content was obtained using the plantlets harvested from 5 mg/L chitosan with 2463.03 ± 0.398 µg/mL compared to the control (without elicitation) with 256.73 ± 0.341 µg/mL (859.39% increase). The results imply that the protocol for the shoot-tip culture of A. paniculata was developed, and that elicitation enhanced the herbage yield and the production of andrographolide.

The lablab bean or ‘Kacang sepat’ is a food crop originating from India and is commonly found in tropical areas such as Indonesia, Thailand and the Philippines due to its adaptability. However, in Malaysia, lablab is grown as a backyard crop, and the area cultivated with this crop is still limited. Lablab has the potential to become a commercial vegetable crop in Malaysia due to its high protein content (18–25%). Therefore, to increase lablab cultivation, good quality seeds are a prerequisite. A key factor in the production of quality seeds is the ideal harvest time of the pods during seed development and maturation and based on identifiable characteristics. The first part of this study was conducted to assess the physical characteristics of lablab pods and seeds of the cultivar Highworth (MDI 12842). Plants were grown using standard cultural practices. Pods and seeds were collected at ten different maturity stages (5, 10, 15, 20, 25, 30, 35, 40, 45, and 50 days after anthesis (DAA)). Pods and seeds showed a significant difference in size and colour at different maturity stages. Seeds harvested at 20 DAA had maximum pod and seed size. The seeds attained physiological maturity (PM) at 30 DAA when the pod colour is light reddish brown and, after being subjected to sun and oven drying, gave maximum germination percentages of 89% and 91%, respectively, while fresh seeds had only 78% germination. Results indicated that the germination percentage of lablab improved, regardless of the drying method used. Therefore, the lablab bean pod colour is a suitable indicator to be used as an easy method for the identification of the best time for pod harvesting for seed production.

Agricultural waste is a type of solid waste that needs to be managed properly. Organic waste can be recycled to produce bokashi fertilizer, which can be used to improve soil health, increase crop production, and sanitize the environment. However, it may contain heavy metals that could be toxic to plants and can pollute the environment if not properly decomposed. This study was designed to evaluate the fertilizer quality of six different bokashi fertilizer ratios (bfrs) over seven- and thirty-day maturation periods. The raw materials used include horse bedding waste (HBW), cow dung (CD), and paddy husk charcoal (PHC) in different ratios, treated with an effective microorganisms (EM4) solution. All the nutrients studied (N, P, K, Mg, and Ca) were significantly affected by the bokashi fertilizer ratios (bfrs). The best bokashi fertilizer ratio was bokashi fertilizer ratio-6 (bfr6), but it was statistically similar to bokashi fertilizer ratio-5 (bfr5). Its N, P, K, Mg, and Ca contents were higher than the control (bfr1) by 133.9%, 225.5%, 196.4%, 105.0%, and 84.7%, respectively. Similarly, all these nutrients were significantly affected by time. N, P, K, and Mg increased by 21.2, 33.0%, 16.4%, and 28.8%, respectively, after 30 days of maturation, with a decrease in Ca only 2.4%, which was not significant A germination index (GI) of 90.1% was obtained using cabbage seeds. The heavy metals result and germination bioassay confirmed the safety and maturity of the bokashi fertilizer. In conclusion, the results revealed that good-quality bokashi fertilizer can be produced within 30 days. Bfrr5 and bfr6 are equally good candidates for producing good-quality bokashi fertilizer for effective crop growth.

A selenium (Se) biofortification program for vegetables has never been attempted in Malaysia. This study was performed to determine the optimum Se fertilization rates for high yield and Se uptake by green spinach (Amaranthus spp.) through a glasshouse experiment and to evaluate the effect of Se application timing on dry matter, growth, and Se accumulation in green spinach through field experiments. Glasshouse experiment conducted with factorial randomized complete block design showed that applying P fertilizer as recommended for green spinach increased yield and Se uptake by the leaves and stems of green spinach. Selenium application in the form of Se(IV) solution at 120 g ha–1 to sandy clay soil was determined to be the optimum application rate, as higher rates reduced plant yield and decreased Se uptake by green spinach. Three cycles of field experiments showed that a single application of 120 g ha–1 Se at 14 d after planting (DAP) produced the optimum results in terms of green spinach growth and dry matter. Although the highest Se accumulation in leaves was observed when Se was applied at 7 DAP (26.04–29.20 μg 10 plants–1), its detrimental effect on yield caused it to be considered an inappropriate time of application. Therefore, with optimum Se accumulation in leaves (21.16–22.38 μg 10 plants–1) and stems (5.89–5.98 μg 10 plants–1), application of Se as Se(IV) solution at 14 DAP at a rate of 120 g ha–1 has been identified as an effective Se fertilization management strategy for producing Se‐biofortified green spinach.

Abiotic and biotic stresses adversely affect rice growth, development and grain yield. Traditional rice breeding techniques are insufficient in modern agriculture to meet the growing population’s food needs on a long-term basis. The development of DNA markers closely linked to target genes or QTLs on rice chromosomes, and advanced molecular techniques, such as marker-assisted selection (MAS), have encouraged the evolution of contemporary techniques in rice genetics and breeding, such as gene pyramiding. Gene pyramiding refers to the act of combining two or more genes from multiple parents into a single genotype, which allows the overexpression of more than one gene for broad-spectrum abiotic and biotic stress resistance. Marker-assisted pedigree, backcrossing and pseudo-backcrossing methods can increase the conventional breeding speed by reducing the number of breeding generations in order to enhance the pyramiding process. Pyramiding is affected by several factors: the number of transferred genes; the range within gene and flanking markers; the number of chosen populations in every breeding generation; the features of genes and germplasms; and the potentiality of breeders to identify the target genes. Modern breeding methods, such as the marker-assisted backcrossing approach, have made gene pyramiding more precise and reliable for the development of stress-tolerant rice varieties in the coming decades. This review presents up-to-date knowledge on gene pyramiding schemes, marker-assisted gene pyramiding techniques, the efficiency of marker-assisted gene pyramiding and the advantages and limitations of gene pyramiding methods. This review also reports on the potential application of marker-assisted selection breeding to develop stress-tolerant rice varieties that stabilize abiotic and biotic stresses. This review will help rice breeders to improve yields by increasing rice productivity under abiotic and biotic stress conditions.

Natural and man-made ecosystems worldwide are subjected to flooding, which is a form of environmental stress. Genetic variability in the plant response to flooding involves variations in metabolism, architecture, and elongation development that are related with a low oxygen escape strategy and an opposing quiescence scheme that enables prolonged submergence endurance. Flooding is typically associated with a decrease in O 2 in the cells, which is especially severe when photosynthesis is absent or limited, leading to significant annual yield losses globally. Over the past two decades, considerable advancements have been made in understanding of mechanisms of rice adaptation and tolerance to flooding/submergence. The mapping and identification of Sub1 QTL have led to the development of marker-assisted selection (MAS) breeding approach to improve flooding-tolerant rice varieties in submergence-prone ecosystems. The Sub1 incorporated in rice varieties showed tolerance during flash flood, but not during stagnant conditions. Hence, gene pyramiding techniques can be applied to combine/stack multiple resistant genes for developing flood-resilient rice varieties for different types of flooding stresses. This review contains an update on the latest advances in understanding the molecular mechanisms, metabolic adaptions, and genetic factors governing rice flooding tolerance. A better understanding of molecular genetics and adaptation mechanisms that enhance flood-tolerant varieties under different flooding regimes was also discussed.

Intercropping is a labor-intensive practice largely adopted by smallholder farmers to increase yield per unit area, cope with crop failures and market fluctuations, meet food preferences and increase farm income. A field experiment was conducted to examine the yield performances and intercropping efficiencies of sweet corn and okra planted in a young rubber plantation. The treatments were arranged in Randomized Complete Block Design (RCBD) with three replications. The treatments ratios were 20% okra + 80% sweet corn (strip and strip relay intercropping) with sole okra and sweet corn as the control. This consists of strips intercropping (okra and sweet corn planted on the same date), strip relay intercropping (sweet corn planted 4 weeks after okra planting), sole okra and sole sweet corn. The highest yield of okra was obtained from strip relay intercropping while the highest yield of sweet corn was produced from the pattern of strip intercropping. However, the economic analysis showed that the strip intercropping recorded the maximum gross margin and costbenefit ratio of RM 17,733.20 ha-1 and 2.09, respectively. Immature rubber growth was unaffected by intercropping with sweet corn and okra crops. Strip intercropping not only resulted in the higher land equivalent ratio (1.29), but also area time equivalent ratio, % land saved and monetary advantage index of 1.14, 22.28% and RM 7,583.50 ha-1, respectively compared with strip relay intercropping. The highest assessment of intercropping based on relative crowding coefficient was recorded by strip intercropping with 4.56. With regard to competition between the intercrops, okra was a more dominant species as measured by the positive value of aggressivity. However, strip intercropping was indicated the lowest competitive ratio than the strip relay intercropping. Thus, this intercropping pattern could be recommended to the farmers for adoption.

Konjac (Amorphophallus muelleri Blume) is a tuberous species that thrives in Malaysian forests and has substantial commercial potential. Despite its importance, agronomic practices that suited with local climate is still scarce. Optimizing key agronomic practices such as shade and K rates require a clear understanding of how they affect plant growth and nutrient dynamics, and how these effects, in turn, influence yield. A field experiment was conducted and treatments of four K rates–0 (T1), 75 (T2), 150 (T3), 225 (T4) kg [ha.sup.-1] were nested in three shading rates–0%, 50%, 70%. Under each shading, each K rate was arranged in a randomized complete block design (RCBD) with four replicates. Regardless of K rates, plants under 70% shading had enhanced growth (leaflet area, petiole diameter), 33.1% more rapid K uptake and 27.9% higher corm yield (P [less than or equal to] 0.01; [alpha] = 0.05), attributed to 15.71% higher photosynthesis rates compared to plants under 50% shading. For nested effects, under 70% shading, T3 was optimum since K uptake and growth was delayed in T4 plants and the corm yield showed nonsignificant difference to T3. Under 50% shading, yield in T4 plants were significantly (P [less than or equal to] 0.01; [alpha] = 0.05) higher (46.4%) compared to T3 plants, demonstrating that under less favourable shading, higher K rate (225 kg [ha.sup.-1]) is needed to increase the yield. Soil K content across growth stages follows quadratic function and the optimum time for K fertilization which can enhance its uptake efficiency under the recommended 70% shading rate is 9-10 wk after planting. Key words: Dry matter, fertilizer, konjac, potassium rates, potassium uptake, shading.