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Motivated by recent work on rotating black hole shadow (Chang and Zhu in Phys Rev D 101:084029, 2020), we investigate the shadow behaviours of rotating Hayward–de Sitter black hole for static observers at a finite distance in terms of astronomical observables. This paper uses the newly introduced distortion parameter (Chang and Zhu in Phys Rev D 102:044012, 2020) to describe the shadow’s shape quantitatively. We show that the spin parameter would distort shadows and the magnetic monopole charge would increase the degree of deformation. The distortion will increase as the distance between the observer and the black hole increases, and distortion reduces as the cosmological constant increases. Besides, the increase of the spin parameter, magnetic monopole charge and cosmological constant will cause the shadows shrunken.

In recent decades, limb-salvage surgery has replaced amputation as the first choice for the treatment of bone tumors around knee. After tumor resection, there are a variety of reconstruction methods for us to choose, including autograft or allograft, inactivation and reimplantation, artificial prosthesis replacement, and allograft-prosthesis compound reconstruction. Compared with other reconstruction methods, artificial prosthesis reconstruction has some advantages: relatively simple, early weight bearing, fewer early complications, and good function in the early and mid-term follow-up. After decades of continuous improvements, the design of tumor prosthesis has reached a relatively mature stage, and the failure rate of prosthesis has also been declining year by year. However, artificial prostheses also have multiple complications such as infection, aseptic loosening, prosthetic breakage, and patients sometimes face the risk of revision or amputation. Therefore, clinicians need to deeply understand the characteristics of related complications and the principles of treatment.

Compatibility between host cells and heterologous pathways is a challenge for constructing organisms with high productivity or gain of function. Designer yeast cells incorporating the Synthetic Chromosome Rearrangement and Modification by LoxP-mediated Evolution (SCRaMbLE) system provide a platform for generating genotype diversity. Here we construct a genetic AND gate to enable precise control of the SCRaMbLE method to generate synthetic haploid and diploid yeast with desired phenotypes. The yield of carotenoids is increased to 1.5-fold by SCRaMbLEing haploid strains and we determine that the deletion of YEL013W is responsible for the increase. Based on the SCRaMbLEing in diploid strains, we develop a strategy called Multiplex SCRaMbLE Iterative Cycling (MuSIC) to increase the production of carotenoids up to 38.8-fold through 5 iterative cycles of SCRaMbLE. This strategy is potentially a powerful tool for increasing the production of bio-based chemicals and for mining deep knowledge. The SCRaMbLE system integrated into Sc2.0’s synthetic yeast chromosome project allows rapid strain evolution. Here the authors use a genetic logic gate to control induction of recombination in a haploid and diploid yeast carrying synthetic chromosomes.

Phytoplankton growth in the western subarctic Pacific is strongly influenced by the supply of bioavailable iron (BFe), which is primarily regulated by atmospheric deposition that was most prominent in spring, weak in summer, and negligible in other seasons. The seasonal phytoplankton responses on these events were investigated using a coupled physical‐biogeochemical model. In fall and winter, light limitation and BFe dilution caused by deep mixing suppressed phytoplankton growth following such events. However, as the mixed layer shallowed in spring, BFe concentrations in the upper ocean increased sharply, triggering a rapid increase in diatom biomass. In contrast, summer exhibited no significant enhancement of diatom biomass due to mesozooplankton grazing pressure, while picoplankton instead dominated the phytoplankton increase. These findings underscore the importance of accounting for local physical and biological factors when projecting the impacts of atmospheric deposition on marine biogeochemical cycles under global climate change.

The spatiotemporal variability of oceanic fronts in the Indonesian seas was investigated using high-resolution satellite observations. The study aimed to understand the underlying mechanism driving these fronts and their impact on chlorophyll-a variability. A high value of frontal probability was found near the coasts of major islands, exhibiting a distinct seasonal cycle with peaks occurrences during austral winter. The distribution variability of chlorophyll-a was generally consistent with the presence of active frontal zones, although a significantly positive relationship between fronts and chlorophyll-a was limited to only some specific areas, e.g., south Java Island and the Celebes Sea. Wind-driven upwelling played a major role in front generation in the Java upwelling region and enhanced frontal activity can promote the growth of phytoplankton, leading to higher chlorophyll-a. Furthermore, the study demonstrated that wind patterns preceded variations in front probability and chlorophyll-a by approximately two months. This lag suggests that the spatiotemporal variability of fronts and chlorophyll-a in this region is primarily influenced by the monsoon system. In addition, the sea surface temperature (SST) simultaneously modulated the chlorophyll-a variability. Negative SST anomalies were typically associated with positive anomalies in front probability the chlorophyll-a in most areas. Notably, the interannual variability of fronts and chlorophyll-a are prominent in the Java upwelling region. During El Niño years, this region experienced an enhanced monsoon, resulting in a negative SST anomaly alongside positive anomalies in front probability and chlorophyll-a. A comprehensive description and underlying dynamics of frontal activity in the Indonesian seas are provided by this study. The findings are helpful to delineate the variability in chlorophyll-a, thereby facilitating the future understanding of local primary production and the carbon cycle.

The mesoscale eddies off the coast of Chile significantly impact the distribution of local chlorophyll and the development of marine ecosystem. Multiple processes, including eddy trapping, pumping, advection, Ekman-pumping, and submesoscale dynamics, exert their impacts simultaneously on transport of water masses at different distances with respect to the eddy center. The cyclonic (anticyclonic) eddies are generally characterized by upwelling (downwelling) within the eddy, which elevates (depresses) chlorophyll inside the eddy. Outside the eddy periphery, multiple processes are involved simultaneously, but their corresponding influences on chlorophyll are not well identified. In this study, the amplitudes of cyclonic and anticyclonic eddies are distinguished as positive and negative values, respectively. A linear regression method is applied to seek the connection between eddy's amplitude and chlorophyll distribution at different locations w.r.t. the eddy center. The regression slope between eddy amplitude and chlorophyll anomaly is found to be negative in the eddy interior and along the periphery, which gradually changes to positive away from the periphery. The location where the response of chlorophyll to an eddy switches its sign is defined as the transition zone. The location of the transition zone varies with offshore distance and is impacted by topography, such as the presence of islands, which can change the dynamics of eddies. Thus, the distance from eddy center and offshore distance from coast should be taken into consideration when investigating their influences on nutrient transport and chlorophyll distribution.

The trends of the sea surface temperature (SST) and SST fronts in the South China Sea (SCS) are analyzed during 2003–2017 using high-resolution satellite data. The linear trend of the basin averaged SST is 0.31°C per decade, with the strongest warming identified in southeastern Vietnam. Although the rate of warming is comparable in summer and winter for the entire basin, the corresponding spatial patterns of the linear trend are substantially different between them. The SST trend to the west of the Luzon Strait is characterized by rapid warming in summer, exceeding approximately 0.6°C per decade, but the trend is insignificant in winter. The strongest warming trend occurs in the southeast of Vietnam in winter, with much less pronounced warming in summer. A positive trend of SST fronts is identified for the coast of China and is associated with increasing wind stress. The increasing trend of SST fronts is also found in the east of Vietnam. Large-scale circulation, such as El Niño, can influence the trends of the SST and SST fronts. A significant correlation is found between the SST anomaly and Niño3.4 index, and the ENSO signal leads by eight months. The basin averaged SST linear trends increase after the El Niño event (2009–2010), which is, at least, due to the rapid warming rate causing by the enhanced northeasterly wind. Peaks of positive anomalous SST and negatively anomalous SST fronts are found to co-occur with the strong El Niño events.

Liquid lens offers a novel approach to achieving large depth of field, wide viewing angle, high speed, and high-quality imaging in zoom optical systems. However, the aperture and reliability limit the lens’s performance in various optical applications. The liquid material is crucial for the reliability of the large-aperture liquid lens. To solve the dielectric failure problem associated with the large aperture, we first reveal the mechanism of dielectric failure based on the transport properties of electrolyte solutions and the impact of electrochemical reaction rates from physical chemistry so as to propose a theoretical method to suppress dielectric failure fundamentally. Based on this theory, we develop a series of non-aqueous organic solutions to suppress high-voltage dielectric failure. Next, we identify the optimal formulation for comprehensive optical performance and fabricate a centimeter-level large-aperture electrowetting liquid lens. This lens features an optical power variation range of −11.98 m −1 to 12.93 m −1 , with clear and high-quality imaging function, which can enlarge the field of view and depth adjustment range of holographic reconstructions while maintaining excellent edge clarity of the reconstructed images. The proposed centimeter-level large-aperture non-aqueous electrowetting liquid lens effectively suppresses dielectric failure under high voltage, demonstrates excellent optical performance, and holds exciting potential for applications in 3D display, precision measurement, biomedical observation, and more. A non-aqueous electrowetting liquid lens with centimeter-level large aperture based on dielectric failure suppression principle is proposed.

It is well established that human marrow stromal cells (hMSCs) can directly migrate towards tumor microenvironments associated with tumor formation and intracellular communication. Gene regulatory networks in tumors may be targeted by microRNAs (miRNAs), especially those derived in exosomes from hMSCs. However, the potential functional roles of hMSCs in glioma cell growth still remain controversial. Therefore, this study aimed at exploring the regulatory mechanisms of hMSC exosomal microRNA-375 (miR-375) in glioma. Microarray analysis was used to initially screen out glioma-related genes. The interaction between miR-375 and solute carrier family 31 member 1 (SLC31A1) was confirmed by dual-luciferase reporter gene assay. miR-375 and SLC31A1 expression in glioma cells were determined. Glioma cells were initially exposed to exosomes derived from hMSCs treated with miR-375. Subsequently, the rates of proliferation, migration, invasion and apoptosis were determined in glioma cells using in vitro assays. The effects of exosomal miR-375 from hMSCs on tumor growth in vivo were also measured using xenograft tumor in nude mice. We found that miR-375 and SLC31A1 showed significantly lower and higher expression of glioma cells respectively. Additionally, restored miR-375 expression resulted in suppressed cell proliferation, migration and invasion, and increased apoptosis by targeting SLC31A1. Next, in vitro experiments demonstrated that hMSC-derived exosomes overexpressing miR-375 promoted apoptosis while suppressing proliferation, migration and invasion. Furthermore, in vivo experiments confirmed the negative regulatory effects of hMSC-derived exosomes with overexpressed miR-375. We conclude that exosomal miR-375 from hMSCs inhibits glioma cell progression through SLC31A1 suppression, and ultimately serves as a potential target in the treatment of gliomas.

Pancreatic cancer is an aggressive malignancy with poor prognosis. Recent advances in chimeric antigen receptor (CAR)-T cell immunotherapy have offered renewed hope. The pivotal factors determining the effectiveness of CAR-T cells in the clinic is the selection of a reliable target and a specific ectodomain of CAR molecule. EpCAM is widely expressed in epithelial tumors, including pancreatic cancer, and is used as a tumor target in clinical trials of CAR-T cell therapy for gastric and colorectal cancers. However, the feasibility of using EpCAM CAR-T cells in pancreatic cancer still needs to be verified. In this work, we reported novel EpCAM CAR-T cells with a fully human single-chain variable fragment and evaluated their cytotoxic effects in a panel of pancreatic adenocarcinoma cell lines, three-dimensional tumor spheroid models and patient-derived organoids, and two xenograft mouse models. We demonstrated that EpCAM CAR-T cells exhibited potent and specific anti-tumor activity against EpCAM positive pancreatic cancer in vitro and in vivo, without adverse effects by systematic delivery in xenograft mouse models. Our preclinical results provided evidence of the efficacy and feasibility of EpCAM CAR-T cells for the immunotherapy of pancreatic cancer.