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Phospholipids are major components of biological membranes. They play an essential role in intracellular signaling and organelle dynamics; however, the availability of suitable lipid-specific probes is limited, which has hindered studies on their spatial distribution and functional dynamics in living cells. Previously, we demonstrated that octadecyl rhodamine B chloride (R18) is transported to the endoplasmic reticulum via nonvesicular membrane transport. In this study, we showed that R18 is internalized in a phosphatidylethanolamine (PE)-dependent manner in vivo. The internalization of R18 in Saccharomyces cerevisiae is blocked in PE-deficient mutants, but restored by ethanolamine supplementation, which suggests strict PE dependence. Moreover, R18 delivered to vacuoles through autophagy was not terminally retained, but underwent Pep4- and Atg15-dependent export from the vacuoles. The exported R18 was then redirected to endosomes following prolonged autophagy. These results suggest that R18 may serve as an indicator of PE dynamics and vacuole–endosome lipid transport, which contributes to lipid homeostasis inside vacuoles.Key words: autophagy, in vivo lipid dynamics, octadecyl rhodamine B (R18), phospholipase, phospholipid, vacuole, yeast

Soil heavy metal pollution caused by human activities has become one of the most critical environmental issues with a global concern. Phytoremediation is widely used due to its low cost and environmental friendliness. However, the impact of heavy metals on plant growth remains unclear. This study investigated the effects on the growth and photosynthetic activity of Picris divaricata Vant. under different cadmium concentrations using a hydroponics cultivation system. The results showed that the growth and photosynthetic processes of P. divaricata exhibited a phenomenon of promotion in low Cd concentrations and inhibition in high Cd concentrations. Under a low to medium Cd concentration (≤25 μM), there was no Cd toxicity in terms of plant growth, but high concentrations of Cd inhibited plant growth. The Fe content of leaves gradually increased as the Cd concentration increased; it reached 201.8 mg kg−1 in 75 μM Cd. However, there was no significant difference in Mn between the 75 μM Cd treatment and the control (p > 0.05). The contents of carotenoid ranged between 3.06 and 3.26 mg/g across the different Cd treatments, showing no significant differences. The treatment with 5–75 μM Cd did not directly affect the photosynthesis of P. divaricata. Higher Cd concentrations reduced the stomatal density on the of P. divaricata leaves, resulting in stomatal and mesophyll conductance limitations, indirectly affecting P. divaricata photosynthesis. These research results provide a reference for evaluating and selecting heavy metal tolerant plants and provide environmentally friendly approaches to remediate heavy metal pollution.

In this paper, we prove the existence and uniqueness of solutions for a singular fractional differential equation boundary value problem with p-Laplacian operator. The main results of this paper are obtained by constructing the monotone iterative sequences of upper and lower solutions and applying the comparison result. Finally, we also provide an illustrative example in support of the existence theorem. Our results generalize some related results in the literature.

Historical chemical production sites often harbor irregularly distributed solid waste landfills, posing significant environmental risks. Traditional drilling methods, while accurate, are inefficient for comprehensive characterization due to high costs and spatial limitations. This study aims to develop an integrated geophysical drilling approach to accurately delineate the spatial distribution and volume of landfilled solid waste (predominantly organic pollutants) at two decommissioned chemical plant sites (total area: 8954 m2). Methods: We combined (1) geophysical surveys (transient electromagnetic (TEM, 50 profiles, 2936 points), high-density resistivity (HDR, 2 profiles, 192 points), and ground-penetrating radar (GPR, 22 profiles, 1072.1 m)) and (2) systematic drilling verification (136 boreholes, ≤10 m × 10 m density). Anomalies were interpreted through integrating geophysical responses, historical records, and borehole validation. Spatial modeling was conducted using Kriging interpolation in EVS software. The results show that (1) the anomalies exhibited a “sparse multi-point distribution” across zones A2 (primary waste concentration), A4, and A6, which were differentiated into solid waste, foundations, contaminated soil, voids, and cracks; (2) drilling confirmed solid waste at nine locations (A2: “multi-point, small-quantity” residues; A6: contaminated clay layers with garbage) with irregular thicknesses (0.2–1.3 m); (3) TEM identified diagnostic medium–high-resistivity anomalies (e.g., 28–37 m in A4L3), while GPR detected 17 shallow anomalies (only one validated as waste); and (4) the total waste volume was quantified as 266.9 m3. The methodology reduced the field effort by ∼35% versus drilling-only approaches, resolved geophysical limitations (e.g., HDR’s volume effect overestimating the thickness), and provided a validated framework for efficient characterization of complex historical landfills.

Good daylighting performance positively affects students’ physical and mental health, learning efficiency, and the building’s energy-saving capability. Due to the terrace classroom having ample space, large capacity, the ability to avoid obstructing sight, and the ability to meet various use needs, it is the most important place in university buildings. However, research on the daylighting performance of university terrace classrooms is limited, leading to a lack of quantitative guidance in early design stages. This study aims to explore the effects of interior space and window geometry of terrace classrooms in universities in severe cold regions on daylighting performance. This research took Shenyang as an example; spatial daylight autonomy (sDA300,50%) and useful daylight illuminance (UDI100–2000) were selected as daylighting performance evaluation indices. Based on the Grasshopper parametric platform, the simulation was carried out using Ladybug and Honeybee plugins. Correlation and regression analyses revealed the relationship between interior space and window geometry parameters and the evaluation indices. The results showed the following: window-to-floor ratio (WFR), classroom height (Htc), window height (Hw), window-to-wall ratio (WWR), classroom width (Wtc), and window width (Ww) have positive effects on improving the daylight sufficiency of the terrace classrooms facing each orientation, and the degree of the effect decreases in order. To ensure the overall daylighting performance, the Wtc can be maximized. The width of walls between windows for south-facing and west-facing classrooms should be 0.9 m. The WWR and WFR for south-facing classrooms should be 0.3–0.5 and 0.11–0.14, respectively. The WWR and WFR for north-facing classrooms should be 0.6–0.7 and 0.14–0.20, respectively. Prediction models are established for the sDA300,50% and UDI100–2000 of the terrace classrooms facing each orientation.

The unique climate and the landscape of severely cold regions in winter attract many tourists. The outdoor thermal environment affects the space use and the tourist experience, becoming one of the key factors in the design of tourist attractions. The outdoor thermal comfort of tourists from different regions should be considered, but it has been poorly studied in winter in severely cold regions. This paper explores the differences in outdoor thermal comfort in winter between local and non-local tourists through the field measurement of the thermal environment and a questionnaire survey of thermal comfort at tourist attractions in Harbin, China. The results show that the proportion of local tourists who expect the air temperature and solar radiation to rise in winter is higher than that of non-local tourists. The thermal sensation vote of local tourists is generally higher than that of non-local tourists. When the Physiologically Equivalent Temperature (PET) < −6 °C, the thermal satisfaction of non-local tourists is higher than that of local tourists. When the PET value is −10 °C, the thermal comfort of non-local tourists is the highest. The thermal comfort decreases with the rise or fall of the PET value. When −28 °C < PET < −7 °C, the thermal comfort of non-local tourists is generally higher than that of local tourists. This paper provides a reference and evaluation basis for urban tourist attractions’ outdoor thermal environment design in severely cold regions.

Selecting representative groundwater monitoring wells in polluted areas is crucial to comprehensively assess groundwater pollution, thereby ensuring effective groundwater remediation. However, numerous factors can affect the effectiveness of groundwater monitoring well network optimizations. A local sensitivity analysis method was used in this study to analyze the hydrogeological parameters of a simulation groundwater solute transport model. The results showed a strong effect of longitudinal dispersion and transverse dispersion on the output results of the simulation model, and a good fit between the backpropagation neural network (BPNN)-based alternative model’s results and those obtained using the solute transport simulation model, accurately reflecting the input and output relationship of the simulation model. The optimized groundwater monitoring layout scheme consisted of four groundwater monitoring wells, namely no. 7, no. 16, no. 23, and no. 24. These wells resulted in a groundwater fluoride pollution rate of 98.44%, which was substantially higher than that obtained using the random layout scheme. In addition, statistical analysis of the fluoride groundwater pollution results obtained using the Monte Carlo random simulation highlighted continuous and high groundwater fluoride levels in the second and third pollution sources and their downstream groundwater. Therefore, more attention should be devoted to these sources to ensure the effective remediation of groundwater pollution in the study area.

Through sampling and analysis of 20 groundwater monitoring wells from nine oil storage enterprises in the Jiangbei New District of Nanjing, the pollution characteristics and chemical spatial distribution of total petroleum hydrocarbons (TPH) in the groundwater of the study area were revealed. TPH was detected in all 20 groundwater samples, with concentrations ranging from 0.26 to 90.24 mg/L. A factor analysis identified two principal factors, F1 and F2, representing the biodegradation processes of iron–manganese reduction and sulfate reduction, respectively. A correlation analysis showed that TPH was significantly positively correlated with total dissolved solids (TDS), total hardness, Fe, Mn2+, and oxygen consumption, but its correlation with sulfides and SO42− was not significant. A further multiple regression analysis indicated that the relative contribution rates of electron acceptors followed the order of iron reduction (90.62%) > manganese reduction (9.35%) > sulfate reduction (0.032%), suggesting that TPH biodegradation is primarily dominated by iron–manganese reduction. Additionally, the study found that microbial growth was more robust in freshwater environments, facilitating TPH degradation, whereas saline environments inhibited microbial activity, thereby hindering TPH degradation.

The geographical distributions of Cd and several other heavy metals (HMs) (Hg, Cu, Ni, Pb, Zn, Cr, As, Co, and V) were characterized in 90 (p > 0.05) terra rossa samples across the Anhui karst area. Significant enrichment of HM was observed in this soil, mainly associated with the weathering of Cd-enriched carbonate rocks. Then, this enrichment was developed in 31 profiles. Our investigations revealed pedogenic processes as the dominant factors accounting for the enrichment of Hg, Cu, Ni, As, Co, and V. We also observed that all soil samples had a silty clay texture, with a pH scope of 4.08–8.04 and a median value of 6.50. In addition, the soil samples had relatively high saturation, with basic cations over 6.68%. The enrichment of the HMs based on their distinct factors were as follows: Cd (3.92) > As (2.55) > Zn (1.62) > Ni (1.50) > Cu (1.47) > Pb (1.47) > V (1.43) > Cr (1.23) > Co (1.19) > Hg (1.12). Finally, terra rossa samples derived from carbonate rocks were categorized as Cambisols, Luvisols, and Regosols. The soil profiles of Cambisols and Luvisolsis were less developed, so the HM concentrations were relatively low. The Regosols profile contained the highest total Cd concentration and exhibited a higher capacity to immobilize Cd compared with other soil profiles. Regosols are also characterized by high pH values (scope of 7.05 to 8.22, with an average value of 7.56). The contents of HM also exhibited minor changes across the Regosol, Cambisol, and Luvisol profiles, implying that the karst development degrees of weathering in Anhui were relatively low.

Phospholipids are major components of biological membranes. They play an essential role in intracellular signaling and organelle dynamics; however, the availability of suitable lipid-specific probes is limited, which has hindered studies on their spatial distribution and functional dynamics in living cells. Previously, we demonstrated that octadecyl rhodamine B chloride (R18) is transported to the endoplasmic reticulum via nonvesicular membrane transport. In this study, we showed that R18 is internalized in a phosphatidylethanolamine (PE)-dependent manner in vivo. The internalization of R18 in Saccharomyces cerevisiae is blocked in PE-deficient mutants, but restored by ethanolamine supplementation, which suggests strict PE dependence. Moreover, R18 delivered to vacuoles through autophagy was not terminally retained, but underwent Pep4- and Atg15-dependent export from the vacuoles. The exported R18 was then redirected to endosomes following prolonged autophagy. These results suggest that R18 may serve as an indicator of PE dynamics and vacuole–endosome lipid transport, which contributes to lipid homeostasis inside vacuoles.Key words: autophagy, in vivo lipid dynamics, octadecyl rhodamine B (R18), phospholipase, phospholipid, vacuole, yeast