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Background Alpine ecosystem underlain by permafrost is considered as one of the most vulnerable ecosystems to disturbance, especially the alpine grassland on the Tibetan plateau with an altitude above 4000 m. Plateau pika ( Ochotona curzoniae ) burrowing can create distinctive bare grounds and cause micro-topographical heterogeneity in alpine grasslands. The burrowing-induced changes in microtopography may directly alter plant and soil interactions as well as ecosystem carbon cycle, which have rarely been studied in Tibetan alpine grasslands. Methods To test the responses of ecosystem respiration (Re) to pika burrowing-induced changes in microtopography, we investigated plant characteristics, soil properties and Re from the bare grounds and vegetated grounds in the alpine meadow and steppe on the Tibetan Plateau. Results Our study showed that vegetation cover, species richness, plant biomass, soil moisture (SM), soil organic carbon (SOC), total nitrogen (STN), soil microbial biomass carbon (MBC) and nitrogen (MBN) in the bare grounds were significantly lower than in the vegetated grounds in both alpine meadow and alpine steppe ( P  < 0.05). However, soil temperature and inorganic nitrogen tended to increase in the bare grounds. The growing season Re was significantly lower in the bare grounds than that in the vegetated grounds ( P  < 0.01). Pika burrowing had negative effects on Re and its temperature sensitivity in both alpine vegetations ( P  < 0.05). The relative changes in Re due to burrowing-induced changes in microtopography were positively correlated with the burrowing caused changes of AGB, BGB, SOC and MBC ( P  < 0.05). Pika burrowing-induced changes in soil temperature, soil moisture, plant biomass and microbial biomass are the major factors for the decrease of Re in the bare grounds. Conclusion In view of the large number of pika burrows in the alpine grasslands and the loss of soil organic carbon due to pika bioturbation, the impacts of pika burrowing-induced changes in microtopography on Re must be considered in predicting the carbon cycle in alpine grasslands.

Background and aims Seasonal grazing is a traditional grassland management practice in alpine swamp meadows on the Tibetan Plateau, but little information is available on the interactions between plant diversity and soil carbon and nitrogen sequestration in warm- and cold- season grazed alpine swamp meadows. Methods A multisite survey was conducted to investigate the plant characteristics and soil properties of Tibetan alpine swamp meadows under warm-season grazing (WG) and cold-season grazing (CG). Results Our study showed that plant biomass, litter mass, soil water content (SW), soil available nitrogen, soil microbial biomass carbon and nitrogen were significantly lower in the WG meadows than in the CG meadows ( P  < 0.05). However, plant diversity, species richness and the evenness index tended to increase in the WG meadows. Soil C and N storage were significantly lower in the WG meadows than in the CG meadows ( P  < 0.01). Grazing-induced changes in SW, plant diversity, plant biomass and litter mass were the major factors resulting in the decrease in soil C and N storage. Conclusions Our results indicate that warm-season grazing is beneficial for species diversity conservation, whereas cold-season grazing is suitable for soil C and N sequestration in alpine swamp meadows. Grazing-induced changes in litter mass greatly contributed to variations in plant diversity and soil C and N storage. In view of the accelerated vegetation and soil degradation in alpine grasslands, periodic warm- and cold-season grazing strategies should be considered to maintain alpine swamp meadow sustainability.

Background and aims Changes of soil organic matter (SOM) stability in alpine ecosystems can alter carbon release from the soil and consequently feed back to climate change. We sought to explore the altitudinal patterns of SOM stability and their relationships with climatic, vegetational and edaphic factors. Methods We combined a number of biological, thermal, chemical, molecular and isotopic indices to characterize SOM stability in two soil layers (0–10 and 10–30 cm) of alpine grassland along two altitudinal gradients of 3200–4200 m (Haibei) and 4400–5200 m (Damxung) on the Tibetan Plateau, respectively. Results There were significant altitudinal variations in SOM stability between the two transects. Greater biological, chemical and isotopic indices of SOM stability were observed in the middle altitude in Haibei, while opposite results were observed in Damxung. Except for a decreasing trend of SOM stability along altitudinal gradient in subsurface soils (10–30 cm) in Damxung, there was no significant altitudinal trend of thermal and molecular indices of SOM stability. The multiple indices of SOM stability were mutually associated and all showed greater SOM stability in subsurface soils than in surface soils across regions and altitudes. Conclusions Along the altitudinal gradient, SOM stability showed opposite unimodal patterns in Haibei and Damxung, and had higher values in subsurface soils than in surface soils of the alpine grassland. Climatic, vegetational and edaphic factors collectively control SOM stability rather than just temperature or precipitation.

Climate warming and human activities are modifying plant litter inputs in alpine grasslands, which is predicted to affect ecosystem structure and function. However, the effects of plant litter removal and warming as well as the combined impacts on the ecological functions of alpine grasslands are not well understood. A field experiment was conducted to investigate the effects of experimental warming, litter removal, and their interaction on ecosystem multifunctionality (EMF) of alpine grasslands. Our results demonstrated a significant decrease in plant diversity ( p < 0.05) and vegetation cover ( p < 0.01) under experimental warming treatment, whereas the richness index ( R ) and belowground biomass (BGB) significantly increased under litter removal treatment ( p < 0.05). The interaction effect of experimental warming and litter removal results in a neutralizing effect on the ecological functions in alpine grasslands. Meanwhile, the EMF tended to increase under all treatments of experimental warming, litter removal, and experimental warming-litter removal. However, there are differences in the response of aboveground and belowground multifunctionality to experimental warming and litter removal. The aboveground ecosystem multifunctionality (AEMF) showed a decreasing trend, while belowground ecosystem multifunctionality (BEMF) increased significantly ( p < 0.01) under the experimental warming treatment. In contrast, AEMF and BEMF showed an increasing trend in litter removal treatment. In addition, the study found that litter removal could alleviate the negative effect of experimental warming on multiple ecological functions. These research findings can serve as a reference for maintaining ecosystem functions in alpine grasslands under climate change conditions and provide effective measures to enhance the capacity of grassland ecosystems to respond to climate change. The application of appropriate litter management measures and other nature-based solutions (NbS) to improve ecosystem functions, aiming to adopt sustainable approaches to address environmental challenges, holds significant importance for ecological conservation.

Water use efficiency (WUE) measures the tradeoff between carbon uptake and water consumption in terrestrial ecosystems. It remains unclear how the responses of WUE to drought vary with drought severity. We assessed the spatio-temporal variations of ecosystem WUE and its responses to drought for terrestrial ecosystems in Southwest China over the period 2000–2017. The annual WUE values varied with vegetation type in the region: Forests (3.25 gC kg−1H2O) > shrublands (2.00 gC kg−1H2O) > croplands (1.76 gC kg−1H2O) > grasslands (1.04 gC kg−1H2O). During the period 2000–2017, frequent droughts occurred in Southwest China, and overall, drought had an enhancement effect on WUE. However, the effects of drought on WUE varied with vegetation type and drought severity. Croplands were the most sensitive to drought, and slight water deficiency led to the decline of cropland WUE. Over grasslands, mild drought increased its WUE while moderate and severe drought reduced its WUE. For forests and shrublands, mild and moderate drought increased their WUE, and only severe drought reduce their WUE, indicating that these ecosystems had stronger resistance to drought. Assessing the patterns and trends of ecosystem WUE and its responses to drought are essential for understanding plant water use strategy and informing ecosystem water management.

Precipitation and temperature are major controls on multiple ecosystem functions in alpine grasslands. There is scant evidence for the interactive effects of temperature and precipitation changes on the ecosystem multifunctionality (EMF) in alpine meadows. To explore the interactive effects of temperature and precipitation changes on the EMF in alpine meadows, we transplanted meadow blocks reciprocally among three altitudes (4,650, 4,950, and 5,200 m) on the central Tibetan Plateau. Compared with the home sites (control), the EMF has a trend to increase when meadow blocks were downward transplanted (experimental warming) to the high-precipitation sites but decrease as meadow blocks were downward transplanted to the low-precipitation sites. However, the experimental cooling (upward transplantation) consistently reduced the EMF regardless of the precipitation change. The increase of EMF under the experimental warming was closely related to the variation of both plant and soil functions, whereas the reduction of EMF under the cooling was highly correlated with the decrease of plant function. Our results highlight that climate warming effects on the EMF are greatly associated with precipitation changes in the semi-arid alpine ecosystems.

Future climate may profoundly impact the functioning of terrestrial ecosystems. However, we do not know well how the functioning of different types of grassland ecosystems is associated with variation in temperature and precipitation. Here, we used long‐term field measurements to examine how climatic changes between the 1980s and the 2010s (i.e., growing season temperature, precipitation, habitat moisture index, solar radiation, and sunshine duration) have affected aboveground net primary productivity (ANPP) for all major grassland types in northern China. We found that ANPP consistently declined over the 30‐year period across all types of grassland, on average by about 6.1%. Warming, associated with increased solar radiation and, hence, soil temperature, was the primary factor driving the decrease of ANPP. We further show that ANPP was more sensitive to climate change in alpine and lowland grasslands than in temperate grasslands. Together, our findings indicate that climate warming consistently reduces plant productivity of different types of grassland ecosystems, and emphasize the importance of soil temperature in driving the decline in grassland productivity under climate change. Plain Language Summary Future climate may profoundly impact the functioning of terrestrial ecosystems. However, we do not know well how the functioning of different types of grassland ecosystems is associated with variation in temperature and precipitation. Here, we used long‐term field measurements to examine how climatic changes between the 1980s and the 2010s (i.e., growing season temperature, precipitation, habitat moisture index, solar radiation, and sunshine duration) have affected aboveground net primary productivity (ANPP) for all major grassland types in northern China. We found that ANPP consistently declined over the 30‐year period across all types of grassland, on average by about 6.1%. Warming, associated with increased solar radiation and, hence, soil temperature, was the primary factor driving the decrease of ANPP. We further show that ANPP was more sensitive to climate change in alpine and lowland grasslands than in temperate grasslands. Together, our findings indicate that climate warming consistently reduces plant productivity of different types of grassland ecosystems, and emphasize the importance of soil temperature in driving the decline in grassland productivity under climate change. Key Points There is a universal decrease in aboveground net primary productivity (ANPP) over 30 years across different grassland types, and the magnitude of ANPP decline differed between types The air temperature and soil temperature rather than precipitation were the primary drivers of ANPP decline to climate change The increased air temperature and soil temperature from increased total solar radiation were the main drivers for ANPP decline

Background Intrauterine adhesion (IUA) as a prevalent gynecological disease is developed from infection or trauma. However, therapeutic strategies to repair damaged endometrium are relatively limited. Emerging studies have shed light on the crucial role of endometrial stromal cells (EnSCs) in the process of uterine endometrial regeneration. EnSCs isolated from the uterine endometrium have similar characteristics to mesenchymal stem cells (MSCs). However, it is still unknown whether EnSCs could be used as donor cells to treat IUA. The aim of this study was to evaluate the potential efficacy of EnSCs in treating rat IUA. Methods Human EnSCs were isolated from the endometrial tissue of healthy female donors and subjected to extensive expansion and culture in vitro. Immunofluorescence, flow cytometry, cell proliferation assay, trilineage differentiation experiment, and decidualization assay were used to characterize the biological properties of EnSCs. We evaluated the immunoregulatory potential of EnSCs by analyzing their secreted cytokines and conducting bulk RNA sequencing after IFN-γ treatment. After EnSCs were transplanted into the uterine muscle layer in IUA rats, their therapeutic effects and underlying mechanisms were analyzed using histological analysis, Q-PCR, fertility and pregnancy outcome assay, and transcriptome analysis. Results We successfully isolated EnSCs from the endometrium of human donors and largely expanded in vitro. EnSCs exhibited characteristics of mesenchymal stem cells and retained responsiveness to sex hormones. Following IFN-γ stimulation, EnSCs upregulated the anti-inflammatory cytokines and activated immunosuppressive molecules. Xenogeneic transplantation of EnSCs successfully repaired injured endometrium and significantly restored the pregnancy rate in IUA rats. Mechanistically, the therapeutic effects of EnSCs on IUA endometrium functioned through anti-inflammation, anti-fibrosis and the secretion of regeneration factor. Conclusions Due to their large expansion ability, immunoregulatory properties, and great potential in treating IUA, EnSCs, as a valuable source of donor cells, could offer a potential treatment avenue for injury-induced IUA.

In this study, a Bayesian-based three-cornered hat (BTCH) method is developed to improve the estimation of terrestrial evapotranspiration (ET) by integrating multisource ET products without using any a priori knowledge. Ten long-term (30 years) gridded ET datasets from statistical or empirical, remotely-sensed, and land surface models over contiguous United States (CONUS) are integrated by the BTCH and ensemble mean (EM) methods. ET observations from eddy covariance towers (ETEC) at AmeriFlux sites and ET values from the water balance method (ETWB) are used to evaluate the BTCH- and EM-integrated ET estimates. Results indicate that BTCH performs better than EM and all the individual parent products. Moreover, the trend of BTCH-integrated ET estimates, and their influential factors (e.g., air temperature, normalized differential vegetation index, and precipitation) from 1982 to 2011 are analyzed by the Mann–Kendall method. Finally, the 30-year (1982 to 2011) total water storage anomaly (TWSA) in the Mississippi River Basin (MRB) is retrieved based on the BTCH-integrated ET estimates. The TWSA retrievals in this study agree well with those from the Gravity Recovery and Climate Experiment (GRACE).

Successfully applied in the carbon research area, sun-induced chlorophyll fluorescence (SIF) has raised the interest of researchers from the water research domain. However, current works focused on the empirical relationship between SIF and plant transpiration (T), while the mechanistic linkage between them has not been fully explored. Two mechanism methods were developed to estimate T via SIF, namely the water-use efficiency (WUE) method and conductance method based on the carbon–water coupling framework. The T estimated by these two methods was compared with T partitioned from eddy covariance instrument measured evapotranspiration at four different sites. Both methods showed good performance at the hourly (R2 = 0.57 for the WUE method and 0.67 for the conductance method) and daily scales (R2 = 0.67 for the WUE method and 0.78 for the conductance method). The developed mechanism methods provide theoretical support and have a great potential basis for deriving ecosystem T by satellite SIF observations.