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This article provides a broad overview on the natural polymer-based bio-nanocomposite properties, processing and application. Bio-nanocomposites prepared with natural biopolymers, such as starch and protein, can be formed using a melt intercalation or a solvent intercalation method. Incorporation of layered silicates into the biopolymer matrices results in improved mechanical properties, water vapor barrier properties, and thermal stability of the resulting bio-nanocomposites without sacrificing biodegradability due to their nanometer size dispersion. Consequently, even though natural polymer-based bio-nanocomposite is in its infancy, it has a huge potential in the future.

In this study, sweet potato β-amylase (SPA) was modified by methoxy polyethylene glycol maleimide (molecular weight 5000, Mal-mPEG5000) to obtain the Mal-mPEG5000-SPA modified β-amylase and the interaction mechanism between SPA and Mal-mPEG5000 was investigated. the changes in the functional groups of different amide bands and modifications in the secondary structure of enzyme protein were analyzed using infrared spectroscopy and circular dichroism spectroscopy. The addition of Mal-mPEG5000 transformed the random curl in the SPA secondary structure into a helix structure, forming a folded structure. The Mal-mPEG5000 improved the thermal stability of SPA and protected the structure of the protein from breaking by the surrounding. The thermodynamic analysis further implied that the intermolecular forces between SPA and Mal-mPEG5000 were hydrophobic interactions and hydrogen bonds due to the positive values of ΔHθ and ΔSθ. Furthermore, the calorie titration data showed that the binding stoichiometry for the complexation of Mal-mPEG5000 to SPA was 1.26, and the binding constant was 1.256 × 107 mol/L. The binding reaction resulted from negative enthalpy, indicating that the interaction of SPA and Mal-mPEG5000 was induced by the van der Waals force and hydrogen bonding. The UV results showed the formation of non-luminescent material during the interaction, the Fluorescence results confirmed that the mechanism between SPA and Mal-mPEG5000 was static quenching. According to the fluorescence quenching measurement, the binding constant (KA) values were 4.65 × 104 L·mol−1 (298K), 5.56 × 104 L·mol−1 (308K), and 6.91 × 104 L·mol−1 (318K), respectively.

This study addresses the challenges of early recognition of fruit and vegetable diseases and pests in facility horticultural greenhouses and the difficulty of real-time deployment on edge devices, and proposes a lightweight cross-scale intelligent recognition network, Light-HortiNet, designed to achieve a balance between high accuracy and high efficiency for automated greenhouse pest and disease detection. The method is built upon a lightweight Mobile-Transformer backbone and integrates a cross-scale lightweight attention mechanism, a small-object enhancement branch, and an alternative block distillation strategy, thereby effectively improving robustness and stability under complex illumination, high-humidity environments, and small-scale target scenarios. Systematic experimental evaluations were conducted on a greenhouse pest and disease dataset covering crops such as tomato, cucumber, strawberry, and pepper. The results demonstrate significant advantages in detection performance, with mAP@50 reaching 0.872, mAP@50:95 reaching 0.561, classification accuracy reaching 0.894, precision reaching 0.886, recall reaching 0.879, and F1-score reaching 0.882, substantially outperforming mainstream lightweight models such as YOLOv8n, YOLOv11n, MobileNetV3, and Tiny-DETR. In terms of small-object recognition capability, the model achieved an mAP-small of 0.536 and a recall-small of 0.589, markedly enhancing detection stability for micro pests such as whiteflies and thrips as well as early-stage disease lesions. In addition, real-time inference performance exceeding 20 FPS was achieved on edge platforms such as Jetson Nano, demonstrating favorable deployment adaptability.

In this study, for the first time, we found a Subsurface Low Potential Vorticity Water (SLPVW) to the east of Taiwan Island (122°$\\mathit{{}^{\\circ}}$ –124°E^{\\circ}\\text{E}$ , 21.67°$\\mathit{{}^{\\circ}}$ –23°N$\\mathit{{}^{\\circ}}\\text{N}$ ) via an array comprising 12 current and pressure‐recording inverted echo sounders (CPIESs) from 25 June 2018 to 29 July 2019. This SLPVW exhibits remarkable intraseasonal variability, with an ∼100‐day period east of Taiwan Island, corresponding with the variability in mesoscale eddies. Compared with large‐scale climatological mean circulation, mesoscale eddy can trap SLPVW as a highway westward transport. In addition, the SLPVW impingement results in a significant subsurface velocity variation (about 10 cm/s) in the 200–400 m layer. These findings shed new light on the mechanisms of the intraseasonal variation in the Kuroshio subsurface layer. Plain Language Summary In this study, we directly observed intraseasonal variation of a Subsurface Low Potential Vorticity Water (SLPVW) in the upstream area of the Kuroshio via in‐situ measurements. This SLPVW originated from water masses from the climatological mean North Pacific Subtropical mode water (STMW) formation region. The STMW forms south of the Kuroshio Extension in the North Pacific in late winter, is an important water mass involved in shaping and memorizing climate variability in the global ocean. The SLPVW was transferred westwards over 1,300 km from the STMW formation region to the Kuroshio region by westward‐propagating mesoscale eddies. Compared with large‐scale circulation, trapping by mesoscale eddies serves as a highway for SLPVW transportation. Moreover, SLPVW impingement results in significant intraseasonal variation in the Kuroshio subsurface velocity. This study provides new insight into the dynamics of Kuroshio intraseasonal variations and highlights the importance of interactions between SLPVW and WBCs. Key Points Intraseasonal variation of Subsurface Low Potential Vorticity Water (SLPVW) was observed in the Kuroshio upstream area by a CPIES array SLPVW is trapped and transported westward by eddies from the Subtropical Mode Water climatological formation region Impingement of SLPVW plays a key role in intraseasonal variation of velocity of the Kuroshio in the subsurface layer

The origin, structure, and variability of the Ryukyu Current (RC) have long been debated, mostly due to limited observations. A mooring array, deployed for two years southeast of Miyakojima in the southern portion of the Ryukyu Island chain, has provided, for the first time, data confirming the existence and revealing the characteristics of the RC in that upstream region, including its velocity structure and variability. The observations show a shoreward-intensified current flowing northeastward, with a subsurface core located near the 1,000 m isobath and having a record-long mean speed of up to 19.4 cm s −1 at 500 m depth. Estimated volume transport across the observation section had mean 9.0 Sv (1 Sv = 10 6 m 3 s −1 ) and standard deviation 8.7 Sv. The RC shows significant barotropic character compared with other similar mid-latitude currents.

Lactobacillus plantarum zrx03 was a bacteriocin‐producing strain isolated from infant's feces. The fermentation supernatant produced by this strain could strongly inhibit Escherichia coli JM109 ATCC 67387, Staphylococcus aureus ATCC 25923, and Listeria monocytogenes CICC 21633, in which the diameter of inhibition zone was 12.83 ± 0.62 mm, 15.08 ± 0.31 mm, 6.75 ± 0.20 mm, respectively, compared with lactic acid bacteria N1, N2, M13, M21, M31, and M37. According to amplification of 16S rRNA gene and identification of phylogenetic tree, this strain had a 1,450 bp sequence and 100% identity to the L. plantarum strain. Based on the influence of different protease treatments, such as pepsin, trypsin, papain, and proteinase K on the antimicrobial activity, this antimicrobial substance was considered to be a natural protein. Using bacteriocin produced by this strain as study object of this experiment, it had been extracted from ammonium sulfate precipitation and different organic solvents. The results showed that ethyl acetate was selected as the optimal solution to crude extraction of bacteriocin after comparing ammonium sulfate precipitation method and organic solvent extraction method, such as n‐butanol, n‐hexane, dichloromethane, trichloromethane, in which the diameter of the inhibition zones was above 28 mm. Results also showed the inhibition spectrum of the obtained bacteriocin had a broad spectrum of inhibition which could inhibit Gram‐positive, Gram‐negative, yeast. Especially, it could effectively inhibit S. aureus ATCC 25923, Bacillus subtilis CICC 10002, Bacillus anthracis CICC 20443, E. coli JM109 ATCC 67387, and Salmonella CMCC 541, and the zone diameter of inhibition has reached more than 28 mm. Moreover, it had a good thermal stability which antibacterial activity was retained 70.58% after treatment at 121°C for 30 min, and pH‐stability was between pH 2.0–9.0. These results suggested bacteriocin produced by L. plantarum zrx03 had potential application prospects in food preservation. This study investigated the crude extraction method and basic characteristics of a purified bacteriocin produced by Lactobacillus plantarum zrx03 isolated from infant's feces. This bacteriocin was considered to be a potential resource to develop natural and high‐efficiency preservatives, which could be well used in the food industry.

Oceanic, nonisostatic responses to near 5-day Rossby–Haurwitz atmospheric pressure waves have been observed in open oceans; however, such responses based on observations in marginal seas such as the South China Sea have not been reported, owing to the limited ocean bottom pressure P bot records. The P bot measurements from pressure recording inverted echo sounders (PIESs) at sites in the northern South China Sea revealed a nonisostatic-like response near 5 days, although the coastal-trapped waves (CTWs) appeared to obscure it because their broadband periods include the near 5-day band. Cross-spectral analysis revealed that the PIES P bot records and the sea level (SL) records of Hong Kong all correlate strongly with the atmospheric pressure and winds over the East China Sea. This is indicative of remotely forced CTWs. The PIES P bot records showed higher coherence near 5 days with the zonal low-pass wavelength filters applied to the atmospheric pressure, and the phase analysis results strongly suggest nonisostatic oceanic responses to the westward-propagating Rossby–Haurwitz waves. Effective separation of CTWs and the nonisostatic responses from the P bot records at the near 5-day period was achieved. The oceanic responses to the Rossby–Haurwitz waves in the northern South China Sea were nonisostatic; a 1-mbar change in air pressure resulted in a 1.58-mbar change in P bot with a phase lag of 14.8°. The mean phase speed of CTWs from Hong Kong to station P3 was estimated to be 9.9 m s −1 .

The Gravity Recovery and Climate Experiment (GRACE), and its follow-on mission (GRACE-FO), provides a novel measurement of the variations in ocean bottom pressure (OBP) at global and basin scales, including those in marginal seas. However, these measurements have not yet been validated rigorously for the South China Sea (SCS). In this study, the accuracy in the monthly GRACE-FO mascon solutions in the SCS from the Jet Propulsion Laboratory (JPL), Center for Space Research (CSR), and Goddard Space Flight Center (GSFC) was validated with the results of the comparison with the in situ OBP records from an array of 25 pressure-recording inverted echo sounders (PIESs) that are located west of the Luzon Strait (LS). The correlation coefficient (Cor) and root mean square difference (RMSD) between the 10-month period of GSFC and PIES, spanning from July 2018 to June 2019 (with missing satellite data for August and September 2018), were 0.77 (p-value = 0.005) and 0.41 mbar (1 mbar = 100 Pa), respectively. These values suggest that the accuracy of GSFC in the SCS in this period was substantially better than that of JPL (Cor = 0.35, p-value = 0.16; RMSD = 0.74 mbar) and CSR (Cor = 0.25, p-value = 0.24; RMSD = 0.89 mbar). Moreover, the volume transport anomaly of the SCS abyssal circulation was estimated and compared based on the OBP records from GSFC and PIES observations, indicating that the GRACE-FO OBP (GSFC) can be used to monitor seasonal or longer-period variations in the SCS abyssal volume transport. Additionally, the variations in OBP from GRACE-FO were significantly overestimated on the continental shelf of the SCS, which may be attributed to signal leakage. Our findings provide reliable evidence for the application of long-term, fully covered OBP records from GRACE-FO in the SCS, and also offer a valuable reference for the application of GRACE-FO in other regions.