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Large volumes of product reviews generated by online users have important strategic value for product development. Prior studies often focus on the influence of reviews on customers' purchasing decisions through the word-of-mouth effect. However, little is known about how product developers respond to these reviews. This study adopts a big data analytical approach to investigate the impact of online customer reviews on customer agility and subsequently product performance. We develop a singular value decomposition-based semantic keyword similarity method to quantify customer agility using large-scale customer review texts and product release notes. Using a mobile app data set with over 3 million online reviews, our empirical study finds that review volume has a curvilinear relationship with customer agility. Furthermore, customer agility has a curvilinear relationship with product performance. Our study contributes to innovation literature by demonstrating the influence of firms' capability of utilizing online customer reviews and its impact on product performance. It also helps reconcile inconsistencies found in literature regarding the relationships among the three constructs.

Bactrocera cucurbitae (Coquillett) is an important pest of cucurbit crops and certain vegetables in Asia, the Middle East, Africa and Hawaii. Most studies on B. cucurbitae have focussed on the effects of prolonged high temperature and very few have examined the effects of short-term exposures to high-temperature on behaviour. In this study, short-term of high-temperature treatments of 33°C, 37°C, 41°C and 45°C were maintained for 1-3hr, and long-term, variable high-temperature treatments were established that consisted of experienced one, two and three times high temperatures stages to 31°C, 33°C, 34°C, 35°C, 36°C, 37°C, 41°C and 45°C for 7hr. We compared the effects of the different high temperatures regimes changes treatments on the mating, oviposition and thermotactic taxis of the flies. The results showed that exposure to a 45°C/1hr treatment, delayed both initiation of mating and oviposition for 8 hr relative to the control but mating and was observed 41 times and oviposition 47 times. By comparison, in the control, mating commenced immediately and was observed 38.3 times and oviposition was observed 41.3 times. Under the other treatments, all the indices for the flies declined with the increase in temperature and duration of exposure. Results showed that 1hr of exposure to 45°C significantly stimulated mating, oviposition and thermotactic behaviour of the flies. These results could improve our understanding of the mechanisms responsible for the population dynamics of B. cucurbitae during the high-temperature season.

Background N-glycosylation is a prevalent post-translational modification in eukaryotes, essential for regulating protein secretion. In Saccharomyces cerevisiae , glycosylation mutants have been shown to enhance the secretion of heterologous glycosylated proteins. However, whether these mutants can also increase the secretion of non-glycosylated proteins and whether the growth defects associated with glycosylation mutations can be mitigated remains unclear. This study aimed to characterize and optimize enhanced secretory expression in the promising yeast host Kluyveromyces marxianus by deleting MNN11 , which encodes a subunit of the mannose polymerase II complex responsible for elongating α-1,6-linked mannose chains. Results Compared to wild-type cells, the mnn11 Δ cells significantly increased the secretion activities of four glycosylated enzymes and three non-glycosylated enzymes in flasks, with increases ranging from 29 to 668%. Transcriptomic analysis of mnn11 Δ mutant revealed upregulation of genes related to essential protein secretion processes, including vesicle coating and tethering, protein folding, translocation, and glycosylation. Additionally, genes involved in vacuolar amino acid transport and amino acid biosynthesis were upregulated, suggesting an amino acid shortage, which might contribute to the observed severe growth defect of the mnn11 Δ mutant in a synthetic medium with inorganic nitrogen. Supplementation of the synthetic medium with amino acids or low concentrations of yeast extract alleviated this growth defect, reducing the specific growth rate difference between wild-type strain and mnn11 Δ cells from 65% to as little as 2%. During high-density fermentation, the addition of 0.5% yeast extract substantially reduced the lag phase of mnn11 Δ mutants and increased the secretory activities of α-galactosidase, endoxylanase, and β-glucanase, by 11%, 18%, and 36%, respectively, compared to mnn11 Δ mutant grown without yeast extract. Conclusion In K. marxianus , deletion of MNN11 enhances the secretion of both glycosylated and non-glycosylated proteins by improving key protein secretion processes. The growth defect in the mnn11 Δ mutant is closely tied to insufficient amino acid supply. Supplementing the synthetic medium with low concentrations of organic nitrogen sources effectively alleviates this growth defect and enhances secretory expression. This strategy could be applied to optimize the expression of other glycosylation mutants.

Objective This study aims to assess and compare the six-month postoperative clinical outcomes of Arthroscopic-assisted Uni-portal Spinal Surgery (AUSS), unilateral biportal endoscopy (UBE), and percutaneous interlaminar endoscopic discectomy (PEID) for lumbar spinal stenosis (LSS). Additionally, muscle injury associated with these procedures is evaluated by analyzing changes in creatine kinase (CK) and C-reactive protein (CRP) levels. Methods A total of 288 patients diagnosed with single-segment unilateral LSS and treated between January 2021 and June 2024 were included in this study. Patients were assigned to the AUSS group ( n  = 129), UBE group ( n  = 86), or PEID group ( n  = 73). Surgical parameters, including operative time, incision length, intraoperative blood loss, and postoperative facet joint preservation rate, were recorded. Clinical outcomes were assessed preoperatively and at 3 days, 3 months, and 6 months postoperatively using the Visual Analog Scale (VAS) for back and leg pain, Oswestry Disability Index (ODI), EQ-5D-5 L quality of life index, and the modified Macnab criteria. The extent of muscle injury was quantified through serum CK and CRP levels measured preoperatively and on postoperative days 1, 3, 5, and 7. Descriptive statistics and multiple comparison analyses were used to assess clinical parameters among the three groups. Longitudinal data were analyzed using a generalized mixed linear model. Results The AUSS group demonstrated significantly shorter operative times and smaller incision lengths compared to the UBE and PEID groups ( P  < 0.001). Postoperative VAS scores decreased significantly in all three groups, with the greatest improvement observed in the AUSS group ( P  < 0.05). ODI and EQ-5D-5 L scores indicated superior postoperative quality of life in the AUSS group compared to the UBE and PEID groups ( P  < 0.05). However, no statistically significant differences were observed in the modified Macnab criteria outcomes or postoperative complication rates among the three groups ( P  > 0.05). The PEID group exhibited the lowest postoperative CK and CRP levels as well as the least intraoperative blood loss ( P  < 0.05), suggesting minimal muscle injury. The AUSS group showed lower muscle injury levels than the UBE group ( P  < 0.05). Conclusion Arthroscopic-assisted Uni-portal Spinal Surgery (AUSS), unilateral biportal endoscopic (UBE) technique, and percutaneous endoscopic interlaminar discectomy (PEID) are all effective minimally invasive approaches for lumbar spinal stenosis (LSS). AUSS offers shorter operative time and better preservation of anatomical structures. PEID minimizes intraoperative tissue damage and reduces inflammatory response, while UBE balances visualization with operational flexibility. All three techniques demonstrate good short-term clinical outcomes. The surgical approach should be tailored to each patient’s symptoms and individualized needs. Although AUSS demonstrated certain intraoperative advantages, this study did not show that AUSS is superior to UBE or PEID in clinical efficacy or complication control. As a novel technique, AUSS may improve postoperative pain and quality of life, providing a valuable addition to the minimally invasive treatment options for lumbar spinal stenosis.

Industrial solid waste poses significant environmental and economic challenges due to its volume and potential hazards. Several industrial activities, such as mining, chemical production, textile, food processing, construction, and power plants contribute to industrial solid waste generation. Industrial solid waste can be classified into several categories, including hazardous, non-hazardous, organic, and inorganic waste. Direct release of industrial solid waste into the environment can cause several health impacts. It is a need for effective recycling and utilization of industrial solid waste through proper waste management methods. The recycling technologies for industrial solid waste, include metallic, non-metallic, hazardous, and non-hazardous materials. Effective waste management practices reduce environmental impact, conserve resources, and promote sustainability by converting waste into valuable products and energy. This review provides a comprehensive overview of industrial solid waste management practices aimed at mitigating environmental impacts. Integrating advanced recycling methods can significantly improve the recycling and utilization of industrial solid waste, contributing to a more sustainable and circular economy.

A series of centrifuge model tests was performed to investigate the influence of subgrade surcharge loading on adjacent bridge pile foundations in soft soils, based on the Mingu Road project in Zhongshan City, China. Four surcharge distances (1D, 2D, 3D, and 4D, where D is the pile diameter) were examined to clarify the spatial–temporal evolution of pile–soil interaction. The results show that horizontal displacement, bending moment, and lateral soil resistance of the pile increase over time, exhibiting significant time-dependent behavior characterized by rapid initial growth followed by stabilization. As the surcharge distance increases, these responses decrease markedly, indicating a strong spatial attenuation effect. The bending moment along the pile depth follows a unimodal pattern with a peak at the soft soil layer. In contrast, the lateral soil resistance exhibits a similar trend of increase and decrease with depth. When the surcharge distance exceeds approximately 4D, the additional influence on the pile response becomes small. This study provides physical evidence and theoretical support for the safe design and construction of bridge pile foundations adjacent to road embankments in areas with soft soil.

The differential characteristics with high probability are critical for differential cryptanalysis. The process of searching such differential characteristics, especially the best one, is time-consuming. We believe that the modern hybrid computing systems can be used to accelerate the search process. However, to the best of our knowledge, the existing solutions are not designed for heterogeneous architectures. In this paper, we propose a parallel search algorithm for the best differential characteristic. Our method can be applied to any substitution–permutation network (SPN) block ciphers after making minor modifications. We implemented the proposed parallel search algorithm for PRESENT block cipher and also a sequential version, which based on the classic Matsui’s method, for comparison. The experimental result shows that the parallel algorithm using both CPU and GPU can achieve at least 4.4x and up to 18x speed-up compared to the sequential version.

Androgenetic alopecia (AGA) is a common clinical condition, affecting over 200 million people globally each year. For decades, Minoxidil (Mi) tincture has been the primary treatment for this disease, but its low utilization rate and significant side effects necessitate new therapeutic strategies. Nitric oxide (NO) is a signaling molecule in various physiological processes, including vasodilation, immune responses, and cell proliferation. Herein, we constructed a hyaluronic acid liposome (HL) complex as a novel transdermal delivery system (HL@Mi/NONOate) for NO and Mi, which displayed promising transdermal and hair-regrowth effects. In-depth mechanistic studies revealed three potential pathways of the synergistic AGA therapy. First, NO promoted capillary dilation and accelerated blood flow, thus achieving efficient penetration of Mi. Due to the structural advantage of liposomes, the residence time of the Mi in the skin was prolonged. Moreover, HL@Mi/NONOate promoted cell proliferation and angiogenesis, and upregulated the expression of regulatory factors involved in follicle stem cell differentiation. In the AGA model, HL@Mi/NONOate down-regulated the expression of inflammatory factors, inhibiting the inflammation of follicle and improving the microenvironment of hair regrowth. Concurrently, HL@Mi/NONOate upregulated the expression of Ki67 and PCNA proteins in follicle tissues, inducing follicle regeneration and development, ultimately achieving the synergistic multimodal AGA therapy.

Potassium ion (K + ) dynamics are vital for various biological processes. However, the limited availability of detection tools for tracking intracellular and extracellular K + has impeded a comprehensive understanding of the physiological roles of K + in intact biological systems. In this study, we developed two novel red genetically encoded potassium indicators (RGEPOs), RGEPO1 and RGEPO2, through a combination of directed evolution in Escherichia coli and subsequent optimization in mammalian cells. RGEPO1, targeted to the extracellular membrane, and RGEPO2, localized in the cytoplasm, exhibited positive K + -specific fluorescence response with affinities of 2.4 and 43.3 mM in HEK293FT cells, respectively. We employed RGEPOs for real-time monitoring of subsecond K + dynamics in cultured neurons, astrocytes, acute brain slices, and the awake mouse in both intracellular and extracellular environments. Using RGEPOs, we were able, for the first time, to visualize intracellular and extracellular potassium transients during seizures in the brains of awake mice. Furthermore, molecular dynamics simulations provided new insights into the potassium-binding mechanisms of RGEPO1 and RGEPO2, revealing distinct K + -binding pockets and structural features. Thus, RGEPOs represent a significant advancement in potassium imaging, providing enhanced tools for real-time visualization of K + dynamics in various cell types and cellular environments.

We assessed the incidence and clinical significance of the fetal region of homozygosity (ROH) detected using single nucleotide polymorphism (SNP) array by analyzing clinical information and pregnancy outcomes. We collected data on 6176 mid- and late pregnancies. All fetuses were subjected to SNP array analysis. Fetuses with ROH were analyzed by karyotyping, parental SNP array verification, whole-exome sequencing, and/or placental studies. Eighty-seven ROHs met our reporting thresholds. Thirty-four fetuses were detected from noninvasive prenatal testing-positive results, with the most common detection rate (2.03%). Twenty-four cases were diagnosed using ultrasound abnormalities; fetal growth restriction was the indication with the highest diagnostic rate. Fifteen cases of uniparental disomy in mid- and late pregnancy were identified (0.24%). Nine cases were of ROH accompanied by aneuploidy or pathogenic/likely pathogenic copy number variants with an adverse pregnancy outcome rate of 88.9%. Of the remaining 78 cases, 14 carriers had adverse outcomes (including two cases of imprinting syndrome), 63 had normal development after birth, and one was lost to follow-up. ROH is relatively common in mid- and late-term pregnancies; its incidence is higher than that reported previously. SNP array is effective in assessing ROH and should be combined with multiple techniques to evaluate ROH’s clinical relevance.