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Although miR-10b-3p has been identified to be involved in cerebral ischemia injury, its impact and specific mechanism in cerebral ischemia injury remain unclear. The effects of Mir-10b-3p were investigated by establishing rat and cell models of ischemia/reperfusion (I/R) injury. Oxygen-glucose deprivation/reperfusion (OGD/R) was performed on pheochromocytoma-12 (PC12) cells. MiR-10b-3p expression levels in brain tissues and PC12 cells were detected by qRT-PCR. The impacts of miR-10b-3p on neurological deficits, infarct volume, inflammatory factor expression, in vivo brain water content, cell viability, and cell apoptosis were assessed. The relationship between miR-10b-3p and KLF5 was determined by TargetScan and luciferase reporter assay. The rescue experiments were performed to confirm the role of this axis in cerebral ischemia injury. Mir-10b-3p levels in rat brain tissue and PC12 cells were significantly decreased after I/R injury. MiR-10b-3p overexpression obviously reduced neurological deficits, infarct volume, brain water content, inflammatory factors expression, and neuronal apoptosis in the brain of ischemia-stroked rats. Meanwhile, miR-10b-3p upregulation also inhibited cell viability and apoptosis of OGD/R-induced PC12 cells. Besides, KLF5 was identified as a target of miR-10b-3p, and rescue experiments revealed that KLF5 was involved in the regulation of miR-10b-3p in ischemic injury. Our results demonstrated that miR-10b-3p had the neuroprotective effects against ischemia injury by targeting KLF5 and provided a potential underlying target for ischemic stroke treatment.

Responses of transpiration (E c) to rain pulses are presented for two semiarid tree species in a stand of Pinus tabulaeformis and Robinia pseudoacacia. Our objectives are to investigate (1) the environmental control over the stand transpiration after rainfall by analyzing the effect of vapor pressure deficit (VPD), soil water condition, and rainfall on the post-rainfall E c development and recovery rate, and (2) the species responses to rain pulses and implications on vegetation coverage under a changing rainfall regime. Results showed that the sensitivity of canopy conductance (G c) to VPD varied under different incident radiation and soil water conditions, and the two species exhibited the same hydraulic control (−dG c/dlnVPD to G cᵣₑf ratio) over transpiration. Strengthened physiological control and low sapwood area of the stand contributed to low E c. VPD after rainfall significantly influenced the magnitude and time series of post-rainfall stand E c. The fluctuation of post-rainfall VPD in comparison with the pre-rainfall influenced the E c recovery. Further, the stand E c was significantly related to monthly rainfall, but the recovery was independent of the rainfall event size. E c enhanced with cumulative soil moisture change (ΔVWC) within each dry–wet cycle, yet still was limited in large rainfall months. The two species had different response patterns of post-rainfall E c recovery. E c recovery of P. tabulaeformis was influenced by the pre- and post-rainfall VPD differences and the duration of rainless interval. R. pseudoacacia showed a larger immediate post-rainfall E c increase than P. tabulaeformis did. We, therefore, concluded that concentrated rainfall events do not trigger significant increase of transpiration unless large events penetrate the deep soil and the species differences of E c in response to pulses of rain may shape the composition of semiarid woodlands under future rainfall regimes.

In recent years, the technology of storing and transporting natural gas in the form of hydrate has received a lot of attention. At present, the research on the synthesis of natural gas hydrate for the purpose of storage and transportation is still in the laboratory stage, and its synthesis process is in the design and conception stage. The influencing factors of natural gas hydrate synthesis under pilot-scale conditions are more complex. Moreover, pilot experiments are oriented to actual production, and its economic feasibility and operational convenience have higher requirements. This paper aimed to study the influencing factors of gas hydrate synthesis by spray method under pilot-scale conditions. Under specific conditions of surfactant and pressure, we carried out research on the effects of reaction temperature, different forms of atomizers, high-pressure pump flow, experimental water, and other factors. Experiments show that the optimal synthesis conditions were a temperature of −5 °C, a pressure of 5 MPa, a conical nozzle, a generated gas hydrate as the hydrate of type I structure, and a gas storage capacity of 1:123 (gas–water ratio).

The PRMS_Storm model was built as a storm event, distributed hydrological model for studying the hydrological effects of forest composition and spatial distribution on storm-flow volume and peakflow rates in the Xiangshuixi Watershed in the Three Gorges Reservoir Area, in the Yangtze River Basin in southwestern China. We developed three simulation scenarios based on forest composition and their spatial arrangements across the watershed, including all mixed conifer-evergreen broadleaf forests (Scenario 1), all mixed evergreen broadleaf forests (Scenario 2), and mixed conifer + evergreen broadleaf + shrub forests (Scenario 3). We examined 11 storm events observed during 2002-2005. Compared with the existing forest covers, modeling results suggested that the amount of overland flow was reduced by 21, 23, and 22%, and the interflow increased by 16, 88, and 30%, for Scenarios 1, 2, and 3, respectively. During the same time, peakflow rates were reduced by 20.8, 9.6, and 18.9%, respectively. The reduction of peakflow rates was most significant when rainfall intensity exceeded 0.8 mm/min and events with a short duration and effect was minor when rainfall intensity was below 0.5 mm/min. In general, we found that Scenarios 1 and 3 were preferred for reducing storm-flow volume and peakflow rates due to their higher interception rates, large soil water holding capacity, and higher soil infiltration capacity. The modeled results suggested soil properties are important in affecting the flow processes and thus forest composition and forest spatial distributions will affect storm-flow volume and peakflow rates at the watershed scale. To maximize flood reduction functions of a watershed, high priority should be given to those forest types (Scenarios 1 and 3) in reforestation practices in the study region. This study suggests both forest composition and spatial pattern are important reforestation designs for flood reduction in the Three Gorges Reservoir Area.

A preliminary study of the hydrological effects of forest litter and soils in the Simianshan Mountains was carried out. Results indicate that the annual accumulation of different forest litters is about 6.80-20.21 t/hm^sup 2^ and the maximum water carrying capacity ranges from 1.8 to 4.6 mm. Among them the water carrying abilities of the litter of Lithocarpus glabra and natural deciduous forests are larger than that of Pinus massoniana. A power function relationship exists between the accumulated water-carrying volume and time. An investigation of the physical properties shows that forest soils, to a depth of 1 m, have a powerful water-carrying ability, varying from 7.84 to 18.87 mm. Non-linear regression analysis shows that the soil infiltration rate is significantly correlated with time.[PUBLICATION ABSTRACT]

Soil moisture is an important hydrological variable in reforestation practices in a water-limited region of the Loess Plateau of northwestern China. The objective of this study was to quantify the spatial dynamics of soil moisture on a complex terrain. During 2004-2006, a total of 313 sample points in two kinds of grid (2 x 2 m and 20 x 20 m) were arranged for soil moisture measurements (two soil layers: 0-30 and 30-60 cm) with Time Domain Reflectometry. The geostatistical properties of soil moisture patterns, the variance and correlation structure of the soil moisture, and the effects of terrain factors on soil moisture were analyzed. The results suggested that our sampling grid captured the spatial variability of soil moisture distributions for this complex terrain. Principal Component Analysis and Cluster Analysis statistics showed that soil moisture decreased as slope gradient increased; that sunny aspects (112.5°-292.5°) had relatively lower soil moisture than did shady aspects (292.5°-112.5°); that soil moisture was lowest in the SWW direction and highest in the NWN direction; and that hillslope aspect was the main factor affecting soil moisture in the 0- to 30-cm soil layer, whereas the main factor for the 30- to 60-cm layer was slope gradient. It was found that the relative values of soil moisture for steep slopes (>36%) with shady aspect (292.5°-112.5°), gentle slopes (<36%) with sunny aspect (112.5°-292.5°), and steep slopes with sunny aspect were 99, 82, and 80, respectively - assuming a soil moisture value of 100 for gentle slopes with shady aspect. The results of this study are expected to be relevant to and useful for reforestation planning and design, parameterization of distributed hydrology models, and land productivity assessment in the study region.

In the process of high resolution images orthorectification, an uneven distribution of ground control points in mountainous area results in the phenomenon of ground objects dislocation between orthophotos edges in the overlap area. Followed image mosaic, fusion and interpretation are seriously affected. High-resolution (2.5 m) panchromatic images with a 30% forward overlap of ALOS satellite were acquired over topography complication zones in Beijing mountainous area. After being ortho-rectified in Geomatica PCI 2012, odd or even serial numbered orthophotos on the same satellite track without edge overlapping were mosaiced and then experimental automatic matching tests between mosaic orthophotos and residual orthophotos were carried out in Automatic Registration of ENVI 4.8 to obtain empirical automatic matching parameters and guiding ground control points in the overlap areas. Finally, automatic edge matching operation was conducted by using parameters and guiding GCPs previously acquired. The results showed that mosaic alternate orthophotos in course line or lateral course lines shortened edge matching time effectively. In Automatic Registration module of ENVI 4.8, Search Window Size = 361, Moving Window Size =19, Area Chip Size = 256 were empirical automatic matching parameters of ALOS panchromatic orthophotos. This method can accomplish high resolution orthophotos edge matching fast and accurately.

:  Soil moisture is an important hydrological variable in reforestation practices in a water‐limited region of the Loess Plateau of northwestern China. The objective of this study was to quantify the spatial dynamics of soil moisture on a complex terrain. During 2004‐2006, a total of 313 sample points in two kinds of grid (2 × 2 m and 20 × 20 m) were arranged for soil moisture measurements (two soil layers: 0‐30 and 30‐60 cm) with Time Domain Reflectometry. The geostatistical properties of soil moisture patterns, the variance and correlation structure of the soil moisture, and the effects of terrain factors on soil moisture were analyzed. The results suggested that our sampling grid captured the spatial variability of soil moisture distributions for this complex terrain. Principal Component Analysis and Cluster Analysis statistics showed that soil moisture decreased as slope gradient increased; that sunny aspects (112.5°‐292.5°) had relatively lower soil moisture than did shady aspects (292.5°‐112.5°); that soil moisture was lowest in the SWW direction and highest in the NWN direction; and that hillslope aspect was the main factor affecting soil moisture in the 0‐ to 30‐cm soil layer, whereas the main factor for the 30‐ to 60‐cm layer was slope gradient. It was found that the relative values of soil moisture for steep slopes (>36%) with shady aspect (292.5°‐112.5°), gentle slopes (<36%) with sunny aspect (112.5°‐292.5°), and steep slopes with sunny aspect were 99, 82, and 80, respectively – assuming a soil moisture value of 100 for gentle slopes with shady aspect. The results of this study are expected to be relevant to and useful for reforestation planning and design, parameterization of distributed hydrology models, and land productivity assessment in the study region.

Soil moisture is an important hydrological variable in reforestation practices in a water‐limited region of the Loess Plateau of northwestern China. The objective of this study was to quantify the spatial dynamics of soil moisture on a complex terrain. During 2004‐2006, a total of 313 sample points in two kinds of grid (2 × 2 m and 20 × 20 m) were arranged for soil moisture measurements (two soil layers: 0‐30 and 30‐60 cm) with Time Domain Reflectometry. The geostatistical properties of soil moisture patterns, the variance and correlation structure of the soil moisture, and the effects of terrain factors on soil moisture were analyzed. The results suggested that our sampling grid captured the spatial variability of soil moisture distributions for this complex terrain. Principal Component Analysis and Cluster Analysis statistics showed that soil moisture decreased as slope gradient increased; that sunny aspects (112.5°‐292.5°) had relatively lower soil moisture than did shady aspects (292.5°‐112.5°); that soil moisture was lowest in the SWW direction and highest in the NWN direction; and that hillslope aspect was the main factor affecting soil moisture in the 0‐ to 30‐cm soil layer, whereas the main factor for the 30‐ to 60‐cm layer was slope gradient. It was found that the relative values of soil moisture for steep slopes (>36%) with shady aspect (292.5°‐112.5°), gentle slopes (<36%) with sunny aspect (112.5°‐292.5°), and steep slopes with sunny aspect were 99, 82, and 80, respectively – assuming a soil moisture value of 100 for gentle slopes with shady aspect. The results of this study are expected to be relevant to and useful for reforestation planning and design, parameterization of distributed hydrology models, and land productivity assessment in the study region.

:  The PRMS_Storm model was built as a storm event, distributed hydrological model for studying the hydrological effects of forest composition and spatial distribution on storm‐flow volume and peakflow rates in the Xiangshuixi Watershed in the Three Gorges Reservoir Area, in the Yangtze River Basin in southwestern China. We developed three simulation scenarios based on forest composition and their spatial arrangements across the watershed, including all mixed conifer‐evergreen broadleaf forests (Scenario 1), all mixed evergreen broadleaf forests (Scenario 2), and mixed conifer + evergreen broadleaf + shrub forests (Scenario 3). We examined 11 storm events observed during 2002‐2005. Compared with the existing forest covers, modeling results suggested that the amount of overland flow was reduced by 21, 23, and 22%, and the interflow increased by 16, 88, and 30%, for Scenarios 1, 2, and 3, respectively. During the same time, peakflow rates were reduced by 20.8, 9.6, and 18.9%, respectively. The reduction of peakflow rates was most significant when rainfall intensity exceeded 0.8 mm/min and events with a short duration and effect was minor when rainfall intensity was below 0.5 mm/min. In general, we found that Scenarios 1 and 3 were preferred for reducing storm‐flow volume and peakflow rates due to their higher interception rates, large soil water holding capacity, and higher soil infiltration capacity. The modeled results suggested soil properties are important in affecting the flow processes and thus forest composition and forest spatial distributions will affect storm‐flow volume and peakflow rates at the watershed scale. To maximize flood reduction functions of a watershed, high priority should be given to those forest types (Scenarios 1 and 3) in reforestation practices in the study region. This study suggests both forest composition and spatial pattern are important reforestation designs for flood reduction in the Three Gorges Reservoir Area.