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Lipid accumulation in mammals has been widely studied for decades due to its significant association with obesity in humans and meat quality in livestock animals. Fatty acid transport 1 (FATP1) is an evolutionarily conserved protein that localizes to the plasma membrane to enhance the transportation of fatty acids (FAs). In line with this function, FATP1 is involved in the metabolism of FAs, including their esterification and oxidation. In addition, the expression of FATP1 can be regulated by several energy-related factors, such as insulin and PPAR activators and transcription factors. These events connect FATP1 with cellular lipid accumulation. Recently, several studies have suggested that FATP1 acts as a facilitator in cellular lipid accumulation, whereas others hold a contrary view. Here, we will review these data and probe the possibility that FATP1 acts as a regulator in lipid accumulation, which will provide effective information for studies on the relationship between FATP1 and obesity in humans and meat quality in livestock animals.

This study aimed to investigate the associations and mediating roles of coping styles and perceived discrimination in the relationship between recurrence risk perception and depression among first-ever stroke patients. Convenience sampling was used to select 382 first-ever stroke patients admitted to our hospital between June 2023 and June 2025. General information questionnaires, the Recurrence Risk Perception Scale, Patient Health Questionnaire-9 (PHQ-9), Medical Coping Modes Questionnaire (MCMQ), and Perceived Discrimination Scale (PDS) were used for assessment. A chain mediation model was constructed and tested. A total of 382 questionnaires were distributed, and 375 valid questionnaires were returned, yielding an effective response rate of 98.17%. The total scores for recurrence risk perception, depression, coping style, and perceived discrimination among the 375 first-ever stroke patients were 35.65 ± 7.37, 5.86 ± 1.39, 43.36 ± 5.12, and 20.77 ± 3.67, respectively. Recurrence risk perception was significantly positively correlated with depression, avoidance, resignation, and perceived discrimination, and significantly negatively correlated with confrontation ( P  < 0.05). Patients with stronger perceived discrimination had a higher recurrence risk perception, more severe depression, and were more inclined to adopt negative coping styles. The specific indirect effect through coping style was 0.230, accounting for 17.54% of the total effect; the specific indirect effect through perceived discrimination was 0.214, accounting for 16.32% of the total effect; and the chain mediating effect through coping style and perceived discrimination was 0.044, accounting for 3.36% of the total effect. Coping style and perceived discrimination were associated with recurrent risk perception and depression in patients with first-ever stroke. Healthcare professionals should address patients’ risk perception of recurrence, focus on guiding them toward adopting positive coping styles, and mitigate the negative psychological impact of perceived discrimination.

Severe water deficits due to abnormal climatic conditions can be observed in hydrology and agriculture and can be assessed by various characteristics of the water system that showdifferent responses to climate variability. This paper comprehensively investigates the sensitivities of hydrological (i.e. streamflow and water storage) and agricultural (i.e. plant water availability) water deficits to climate variability at a global scale from a hydrological cycle perspective. The sensitivities of 77 large basins across the globe are quantified by both multiple linear regression (MLR) and the Budyko framework based on a newly released terrestrial water cycle dataset. We find that streamflow and water storage deficits are generally more sensitive to rainfall variation, while plant water availability is more responsive to variations of potential evapotranspiration. The climate sensitivities of the water deficit indices are shown to vary with the wetness index and are shaped by catchment surface properties like water storage capacity. The sensitivities of streamflow deficits to rainfall are higher in wetter regions, while the sensitivities of plant water availability to potential evapotranspiration are higher in drier regions. The findings about the divergent responses in water deficit indices can be conducive to developing region-dependent water resource management strategies to alleviate water deficits under a changing environment.

The adipose-derived stem cells (ASCs) are a valuable resource for regenerative medicine and essential materials for research in fat deposition. However, the isolation procedure of ASCs has not been standardized and needs to be harmonized; differences in proliferation and adipogenic differentiation of ASCs obtained from different fat depots have not been well characterized. In the present study, we compared the efficiency of ASCs isolation by enzymatic treatment and explant culture methods and the proliferation ability and adipogenic differentiation potential of ASCs isolated from subcutaneous and visceral fat depots. The explant culture method was simple and with no need for expensive enzymes while the enzymatic treatment method was complex, time consuming and costly. By the explant culture method, a larger number of ASCs were isolated from subcutaneous and visceral fat depots. By contrast, fewer ASCs were obtained by the enzymatic treatment method, especially from visceral adipose. ASCs isolated by the explant culture method performed well in cell proliferation and adipogenic differentiation, though they were slightly lower than those by the enzymatic treatment method. ASCs isolated from visceral depot demonstrated higher proliferation ability and adipogenic differentiation potential. In total, the explant culture method is simpler, more efficient, and lower cost than the enzymatic treatment method for ASCs isolation; compared with visceral adipose, subcutaneous adipose is easier to isolate ASCs; however, the visceral ASCs are superior to subcutaneous ASCs in proliferation and adipogenic differentiation.

Water contamination by mercury ions (Hg2+) causes irreversible and serious effect on the ambient environment, ecological systems, and human health, necessitating further improvement of Hg2+ monitoring at low concentrations. Here, we proposed a novel surface plasmon resonance (SPR) sensor for Hg2+ detection with desirable advantages of high sensitivity, simple operation, label-free, and low cost, in which the chitosan/poly (vinyl alcohol)/SnO2 composite film was modified on sensing surface as the active layer for sensitivity enhancement. Benefiting from the relatively high refractive index of SnO2 nanoparticles, the evanescent field generated at the metal-solution interface can be significantly enhanced, which results in a 5 times improvement of sensitivity. Through appropriate optimization in the aspects of componential constitutions, the sensor exhibits excellent sensitivity of 25.713 nm/μg/L and ultra-low calculated detection limit of 6.61 ng/L(32.95 pM). Such detection limit is strikingly lower than the limitation (10 nM) in drinking water set by the US Environmental Protection Agency. In addition, the as-prepared sensor presents relatively high selectivity for Hg2+, attributing to plenty of binding sites for specific adsorption produced by functionalized chitosan/poly (vinyl alcohol) composites, which have been furtherly verified by characterization of FTIR and XPS spectra. The proposed sensor also exhibits great repeatability and good time stability for 15 days. This work provides a promising strategy for developing high-performance SPR sensor for Hg2+ detection and a prospective application in environmental monitoring.

Background In China, although buffaloes are abundant, beef is mainly obtained from cattle, and this preference is mainly attributed to the low intramuscular fat (IMF) content of buffalo. Genetic factors are an important driver that affects IMF deposition. Results To reveal the intrinsic factors responsible for the low IMF content of buffalo, mRNA expression patterns in muscle and adipose tissue between buffalo and cattle were characterized by RNA sequencing analysis. The IMF content in Nanyang cattle was higher than that in Xinyang buffalo. A total of 1566 mRNAs expressed in adipose tissue showed differential expression between the longissimus dorsi muscles of buffalo and cattle. Functional annotation suggested a difference in the glycolysis/gluconeogenesis pathway between the two species. The results of RT-qPCR analysis and gain-of-function experiments confirmed the positive association between the IMF content and phosphoenolpyruvate carboxykinase 1 ( PCK1 ) expression in buffalo. In both mouse C2C12 cells and cultured bovine myocytes, the activity of the PCK1 promoter in buffalo is lower than that in cattle. However, in mouse 3T3-L1 adipocytes and cultured bovine adipocytes, the activity of PCK1 in buffalo promoter is higher than that in cattle. Conclusions These results indicate the important role of PCK1 in buffalo IMF deposition and illustrate the differences between buffalo and cattle promoter activity that drive PCK1 expression. This research helps to establish a foundation for further studies investigating IMF deposition in buffalo.

Background The intramuscular fat content and the level of unsaturated fatty acids influence meat quality and nutritional value. microRNAs can participate in lipid metabolic processes. Understanding how novel miRNAs regulate lipogenesis and fatty acid metabolism is key to enhancing buffalo meat quality and nutritional value. Results In this study, miRNA sequencing was conducted on 36 adipose tissues from six anatomical sites in river buffaloes (Murrah). A total of 1,682 miRNAs were identified in 36 adipose tissues, of which 987 were novel and 695 were known miRNAs. Weighted gene co-expression networks analysis (WGCNA) revealed that bta-novel-miR-25336 was highly expressed in sternum subcutaneous adipose tissue (SSAT) and had a high correlation with unsaturated fatty acids (UFA). Functional studies showed that bta-novel-miR-25336 was positively correlated with the proliferation and adipogenic differentiation of both buffalo preadipocytes and intramuscular adipocytes. Additionally, we used bioinformatics analysis and a 3’ untranslated region (3’UTR) luciferase reporter assay to validate acyl-CoA dehydrogenase short chain ( ACADS ) as a target gene of bta-novel-miR-25336. Conclusions In summary, bta-novel-miR-25336 may regulate biological processes associated with cell proliferation, lipid differentiation and unsaturated fatty acid synthesis through ACADS . This study provides a theoretical basis for further elucidating the regulation of lipid metabolism by novel miRNAs in farm animals.

Background Abnormal platelet activation is a key factor in the occurrence and development of thrombotic diseases. However, the physiological mechanisms that underlie platelet homeostasis remain unclear. Oleic acid, one of the most abundant lipids in the human diet, has potential antithrombotic effects. This study aimed to investigate the effects of oleic acid on platelet activation and thrombosis. Methods Platelet aggregation, ATP release, and fibrinogen spread were evaluated to determine the role of oleic acid in platelet activation. A ferric chloride-induced carotid injury model was used to establish the effect of oleic acid on thrombus formation in vivo. Western blotting analysis and transfection experiments were performed to determine the mechanisms involved in this process. Results Oleic acid inhibited platelet aggregation, granule release, and calcium mobilization. Furthermore, it inhibited the spread of platelets on fibrinogen. We also found that oleic acid delayed arterial thrombosis in mice, as demonstrated in a murine model of ferric chloride-induced carotid artery thrombosis. The molecular mechanism of its inhibition of platelet activity may be through the Syk-PLCγ2 and CaMKKβ/AMPKα/VASP pathways. In addition, we demonstrated that the phosphorylation of AMPK at Ser496 was an important mechanism of platelet activation. Conclusions Our study showed that oleic acid inhibits platelet activation and reduces thrombogenesis by inhibiting the phosphorylation of multiple signaling molecules, offering new insights into the research and development of antiplatelet drugs. E5Xy1vH3DfoQAQBKnckQzx Video Abstract

In many places around the world, panevaporation has been detected to decrease with the increase in temperature, which is known as the “panevaporation paradox.” An example of the paradox was found in the Haihe River Basin from 1957 to 2001. To explain the mechanism of the paradox, an approach to quantify the contributions of climate factors to the panevaporation trend has been proposed, in which the individual contribution was defined as the product of the partial derivative and slope of the trend for the concerned variables. Four variables, including temperature, wind speed, solar radiation, and vapor pressure, were selected based on the Penman‐Monteith method to assess their individual contribution to the panevaporation trend. The results showed that an increase in temperature resulted in the increase of panevaporation, but this effect had been offset by an increase in vapor pressure and decrease in wind speed and solar radiation. Wind speed was the dominant factor contributing to panevaporation decreases in the Haihe River Basin.

In order to study the impact of risk aversion characteristics of enterprises on supply chain risk transmission, the risk aversion utility function is introduced, and the risk elasticity coefficient is used to construct a supplier-dominated low-carbon supply chain risk transmission model. Simulation analysis is conducted to investigate the transmission of emission reduction and revenue risks caused by internal and external contingent risk factors. The study reveals that under conditions of market demand uncertainty, the risk transmission effect is unaffected by the risk aversion characteristics of members in the low-carbon supply chain. While the risk-aversion characteristics of suppliers can decrease their own profit risk, they have a negative effect on the profit risk of manufacturers and the emission reduction risk of supply chain nodes. There exists a critical threshold for the impact of the risk-aversion degree of suppliers on their own emission reduction risk transmission effect. When this threshold is exceeded, the emission reduction risk decreases with increasing risk aversion intensity of suppliers, and vice versa. The risk aversion characteristics of manufacturers can weaken the negative effect of supplier risk aversion on the fluctuation risk of manufacturer profits, but they exacerbate the emission reduction risk transmission effect of manufacturers under asymmetric information influence. The findings have important theoretical and practical implications for supply chain risk management.