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• Molybdenum (Mo) is an essential micronutrient for most living organisms, including humans. Cereals such as rice (Oryza sativa) are the major dietary source of Mo. However, little is known about the genetic basis of the variation in Mo content in rice grain. • We mapped a quantitative trait locus (QTL) qGMo8 that controls Mo accumulation in rice grain by using a recombinant inbred line population and a backcross introgression line population. • We identified a molybdate transporter, OsMOT1;1, as the causal gene for this QTL. OsMOT1;1 exhibits transport activity for molybdate, but not sulfate, when heterogeneously expressed in yeast cells. OsMOT1;1 is mainly expressed in roots and is involved in the uptake and translocation of molybdate under molybdate-limited condition. Knockdown of OsMOT1;1 results in less Mo being translocated to shoots, lower Mo concentration in grains and higher sensitivity to Mo deficiency. We reveal that the natural variation of Mo concentration in rice grains is attributed to the variable expression of OsMOT1;1 due to sequence variation in its promoter. • Identification of natural allelic variation in OsMOT1;1 may facilitate the development of rice varieties with Mo-enriched grain for dietary needs and improve Mo nutrition of rice on Modeficient soils.

Al/steel composite plate has a wide application prospect, but great differences in properties between Al and steel are observed. It is difficult to obtain high bonding strength by the traditional cold roll bonding process. Al/steel composite plate was thus prepared by cold roll bonding at a reduction rate of 60% after oxidation treatment on the surface to be composited on the steel side. The heat treatment of holding at 400 °C for 1 h and cooling with the furnace was then adopted. The bonding strength, microstructure, and properties of the Al/steel composite plate before and after annealing were analysed and compared through shear test, bending test, tensile test, and micro-characterization. Results show that the shear strengths of the interface before and after annealing are 100 and 80 MPa, respectively. Although the shear strength of the annealed Al/steel composite plate decreases, the bending and overall tensile properties of the composite plate are improved, showing better mechanical properties.

In recent years, compounds that are active ingredients in herbal medicines have been investigated for their ability to treat cancer. Astragalin may exert anticancer effects through anti-inflammatory, anti-glycosylation, anti-adipogenesis, antioxidant and neuroprotective effects; however, there has been no literature review on the specific mechanism of action of astragalin in cancer, to the best of our knowledge. Therefore, the present review searched and reviewed the literature related to astragalin and cancer, and summarized the possible mechanisms. The results revealed that astragalin affects the proliferation, invasion and angiogenesis of cancer cells through participating in signaling pathways, regulating apoptotic proteins, inactivating oncogenes and suppressor genes, as well as the tumor microenvironment, angiogenesis and other mechanisms of action. In turn, this exerts antitumor effects and reverses chemotherapy resistance. Astragalin is expected to serve a greater role in the prevention and treatment of malignant tumors in the future due to its availability, safety and economy, thus providing new hope for global cancer treatment. Key words: astragalin, Chinese herbal medicine, neoplasms, apoptosis, mortality, broad-spectrum anti-cancer

The purpose of this study was to accurately evaluate the diagnostic efficacy of combined detection of ascitic fluid alpha- l -fucosidase (AFU) and cholesterol (TCH) compared with that of their individual detection. We assayed ascitic AFU activity by colorimetry and TCH level by CHOD-PAP method simultaneously in all 213 cases. Then, we assessed the value of combined detection of AFU and TCH activities with receiver operating characteristic curve (ROC curve), including diagnostic sensitivity, specificity, diagnostic accuracy, positive predict value(PV+), negative predict value(PV−) to see whether these two ascitic fluid biochemical examinations might help in differential diagnosis between malignant and non-tuberculous benign ascites. The mean values of AFU and TCH in malignant group [(164.96 ± 87.72) μmol/lh and (1.65 ± 1.00) mmol/l, respectively] were significantly higher than those in non-tuberculous benign group [(104.02 ± 62.08) μmol/lh and (0.69 ± 0.58) mmol/l, respectively] ( P  < 0.01). The optimal cutoff value of 101.95 μmol/lh for ascitic AFU and 1.04 mmol/l for ascitic TCH resulted in a diagnostic sensitivity of 82.3% and 70.8%, specificity of 63.2 and 83.8%, accuracy of 72.8 and 77.9%, PV+ of 65.3 and 78.2%, PV− of 83.1 and 77.8%, respectively. Combined detection of the two markers, the sensitivity, specificity, accuracy, PV+ and PV− were 86.5, 85.5, 85.9, 83.0, and 88.5%, respectively. Through comparison by Pearson Chi-square, the combined detection of AFU and TCH produced better diagnostic accuracy (85.9%) compared with the individual detection ( P  < 0.05). The combined detection of AFU and TCH produced better diagnostic accuracy in comparison with their individual detection, which is helpful to differential diagnosis between malignant and non-tuberculous benign ascites that may be relatively ideal markers to fit for clinical application in local hospitals.

Roll number and rolling schedule are the important parameters affecting the quality of steel bar straightening，when domestic manufacturer design the straightening equipment, these two parameters design basic is by analogy, so it is necessary to study these two parameters. This paper calculated the straightening roller to obtain reasonable roll number; next step quantitative calculate to obtains the theory rolling schedule, in the meantime actual measurement to obtains the practical rolling schedule of the same equipment；then based on rigid-flexible virtual prototype technology to build straightening system dynamics model. At the theory rolling schedule, and the practical rolling schedule for steel bar straighten emulation, obtained straightness and deviation of different diameter steel bar after straightening. By comparison and analysis the simulation results, we obtain the reasonable roll number and rolling schedule. In this paper, this method have reference value to design the roll number and rolling schedule for similar straightening equipment.

Cryopreservation of few spermatozoa is still a major challenge for male fertility preservation. This study reports use a new micro-straw （LSL straw） for freezing few spermatozoa for intracytoplasmic sperm injection （ICSI）. Semen samples from 22 fertile donors were collected, and each semen sample was diluted and mixed with cryoprotectant in a ratio of 1：1, and then frozen using three different straws such as LSL straw （50-100μl）, traditional 0.25 ml and 0.5 ml straws. For freezing, all straws were fumigated with liquid nitrogen, with temperature directly reducing to -130--140℃. Sperm concentration, progressive motility, morphology, acrosome integrity, and DNA fragmentation index were evaluated before and after freezing. After freezing-thawing, LSL straw group had significantly higher percentage of sperm motility than traditional 0.25 ml and 0.5 ml straw groups （38.5% vs 27.4% and 25.6%, P 〈 0.003）. Sperm motility and acrosomal integrity after freezing-thawing were significantly lower than that of before freezing. However, there was no significant difference in morphology, acrosome, and DNA integrity between the three types of straws （P 〉 0.05）. As LSL straws were thinner and hold very small volume, the freezing rate of LSL straw was obviously faster than 0.25 ml straw and 0.5 ml straws. In conclusion, LSL micro-straws may be useful to store few motile spermatozoa with good recovery of motility for patients undergoing ICSI treatment.

The vertical steel bar bending forming is a kind of new process of bending method. The bending speed, bending radius and clamping length H which is the parameters of vertical steel bar bending machine , is directly affect the quality of bending forming parts. This paper calculated the length of reinforcement before being incised and the springback angle of bending steel bar which obtained the reasonable cutting length and bending Angle; Then based on rigid-flexible virtual prototype technology to build the dynamics model of vertical steel bar bending system. Through simulation analysis ,it obtained the relationship between bending speed, bending radius , clamping length H and forming quality of bending steel bar. In this paper, the analysis method have reference value to the design of similar steel bar bending machines.

Background： Lack of fluoroscopic landmarks can make valve deployment more difficult in patients with absent aortic valve （AV） calcification. The goal of this article was to evaluate the feasibility and effectiveness oftranscatheter implantation of a valved stent into the AV position of a goat, assisted with a microcatheter which provides accurate positioning of coronary artery ostia to help valved stent deployment. Methods： The subjects were 10 healthy goats in this study. A microcatheter was introduced into the distal site of right coronary artery （RCA） through femoral artery sheath. A minimal thoracic surgery approach was used to access the apex of the heart. The apex of the left ventricle was punctured： a delivery catheter equipped with the valved stent was introduced over a stiffguidewire into the aorta arch. We could accurately locate the RCA ostia through the microcatheter placed in the RCA under fluoroscopy. After correct valve position was confirmed, the valved stent was implanted after rapid inflation of the balloon. The immediate outcome of the function of the valved stents was evaluated after implantation. Results： All ten devices were successfully implanted into the AV position of the goats. Immediate observation after the procedure showed that the valved stents were in the desired position after implantation by angiography, echocardiogram. No obstruction of coronary artery ostia occurred, and no moderate to severe aortic regurgitation was observed. Conclusions： When the procedure of transcatheter implantation of a balloon-expandable valved stent into the AV position of goats is assisted with microcatheter positioning coronary artery ostia, the success rate of operation can be increased in those with noncalcified AV.

This systematic review was performed to compare the efficacy and complications of transperineal （TP） vs. transrectal （TR） prostate biopsy. A systematic research of PUBM ED, EMBASE and the Cochrane Library was performed to identify all clinical controlled trials on prostate cancer （PCa） detection rate and complications achieved by TP and TR biopsies. Prostate biopsies included sextant, extensive and saturation biopsy procedures. All patients were assigned to a TR group and a TP group. Subgroup analysis was performed according to prostate-specific antigen （PSA） levels and digital rectal examination （DRE） findings. The Cochrane Collaboration＇s RevMan 5.1 software was used for the meta-analysis. A total of seven trials, including three randomized controlled trials （RCTs） and four casecontrol studies （CCS）, met our inclusion criteria. There was no significant difference in the cancer detection rate between the sextant TR and TP groups （risk difference （RD）, -0.02; 95% confidence interval （CI）, -0.08-0.03; P=0.34）. Meta-analysis for RCTs combined with CCS showed that there was no difference in the cancer detection rate between the extensive TR and TP group （RD, -0.01; 95% CI, -0.05-0.04; P=0.81）. There was no significant difference in PCa detection rate between the saturation TR and TP approaches （31.4% vs. 25.7%, respectively;P=0.3）. There were also no significant differences in cancer detection between the TR and TP groups in each subgroup. Although the data on complications were not pooled for the meta-analysis, no significant difference was found when comparing TR and TP studies. TR and TP biopsies were equivalent in terms of efficiency and related complications. TP prostate biopsy should be an available and alternative procedure for use by urologists.

The wind field plays a decisive role in haze generation and dissipation processes over the Beijing-Tianjin-Hebei (BTH) region. Although geographically the BTH region is under the influence of the East Asian winter monsoon (EAWM), this study finds that common indices of the EAWM cannot adequately describe the actual wind speed changes in the BTH region. Thus, observational data are used to analyze the interannual variations of the winter wind field over the BTH region. The results show that the average winter wind speed is 2.0 m s −1 , with a slight rate of decline of 0.01 m s −1  yr −1 . In most cases, strong-wind years correspond to negative sea surface temperature (SST) anomalies over the tropical Pacific, whereas weak-wind years correspond to positive SST anomalies. Moreover, correlation and composite analyses show that the interannual variability is affected by multiple factors, including the following: (1) the pressure gradient in the high and middle latitudes of the Northern Hemisphere, as in strong-wind years the pressure gradient helps cold air move from high latitudes to middle latitudes; (2) the skin temperature in Eurasia, as low skin temperature in Eurasia in strong-wind years is conducive to the accumulation of cold air; and (3) the SST of the tropical Pacific east of the Philippines, as in strong-wind years the high temperature of this area affects the BTH region through anticyclonic activity and associated tropical circulation systems.