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Jasmonate and ethylene act synergistically in the regulation of plant defence but antagonistically in other aspects. Here the recent findings regarding the mechanisms for their dual interactions are reviewed. Jasmonate (JA) and ethylene (ET) are considered to be two essential plant hormones helping plants to tolerate infections by necrotrophic fungi. Phenotypic observations and marker gene expression analysis suggest that JA and ET act synergistically and interdependently in these defence responses. However, JA and ET also interact in an antagonistic way. JA represses ET-induced apical hook formation, while ET inhibits JA-controlled wounding responses. Although these physiological observations have been reported for more than a decade, only recently have the underlying molecular mechanisms been uncovered. Here, I review the recent advances in the understanding of these two hormone interactions and further discuss the biological significance of these apparently opposite interactions between these two hormones in orchestrating plant growth and development.

Composite structures made of 2 mm-thick titanium and 10 mm-thick carbon steel are widely used in infrastructures such as long-distance gas transportation. However, cracking, which is caused by intermetallic compounds (ICs), is a dominate failure mode in welds of this structure. Thus, a common way to improve the in-service life of is reduce the number of ICs. In this paper, we employ a novel hybrid welding method to fabricate composite structures of TA 2 titanium and Q235 carbon steel. Specifically, Ti and carbon steel is welded by laser and double Cold Metal Transfer (CMT) welding, respectively. The microstructure near the interface of Ti and steel is then examined using SEM, EBSD, EDS, with emphasis on the ICs in terms of chemical elements and morphologies. Results show that FeTi and Fe 2 Ti are the main ICs near the interface, and responsible for the failure of the welds. The effect of welding heat input on the formation of ICs is investigated as well. Results show that ICs are smaller when the heat input is low. Under low heat input circumstance, the tensile strength of the weld can reach up to 420 MPa.

The clinical success of immunotherapy that inhibits the negative immune regulatory pathway programmed cell death protein 1/PD-1 ligand (PD-1/PD-L1) has initiated a new era in the treatment of metastatic cancer. PD-L1 expression is upregulated in many solid tumors including lung cancer and functions predominantly in lactate-enriched tumor microenvironments. Here, we provided evidence for PD-L1 induction in response to lactate stimulation in lung cancer cells. Lactate-induced PD-L1 induction was mediated by its receptor GPR81. The silencing of GPR81 signaling in lung cancer cells resulted in a decrease in PD-L1 protein levels and functional inactivation of PD-L1 promoter activity. In addition, GPR81-mediated upregulation of PD-L1 in glucose-stimulated lung cancer cells that recapitulates the enhanced glycolysis in vivo was dependent on lactate dehydrogenase A (LDHA). We also demonstrated that activation of GPR81 decreases intracellular cAMP levels and inhibits protein kinase A (PKA) activity, leading to activation of the transcriptional coactivator TAZ. Interaction of TAZ with the transcription factor TEAD was essential for TAZ activation of PD-L1 and induction of its expression. Furthermore, we found that lactate-induced activation of PD-L1 in tumor cells led to reduced production of interferon-γ and induction of apoptosis of cocultured Jurkat T-cell leukemia cells. Our findings reveal an unexpected role of lactate in contributing to tumor cell protection from cytotoxic T-cell targeting and establishes a direct connection between tumor cell metabolic reprograming and tumor evasion from the immune response.

The successful synthesis of superconducting infinite-layer nickelate thin films with the highest T c  ≈ 15 K has ignited great enthusiasm for this material class as potential analogs of the high- T c cuprates. Pursuing a higher T c is always an imperative task in studying a new superconducting material system. Here we report high-quality Pr 0.82 Sr 0.18 NiO 2 thin films with T c onset  ≈ 17 K synthesized by carefully tuning the amount of CaH 2 in the topotactic chemical reduction and the effect of pressure on its superconducting properties by measuring electrical resistivity under various pressures in a cubic anvil cell apparatus. We find that the onset temperature of the superconductivity, T c onset , can be enhanced monotonically from ~17 K at ambient pressure to ~31 K at 12.1 GPa without showing signatures of saturation upon increasing pressure. This encouraging result indicates that the T c of infinite-layer nickelates superconductors still has room to go higher and it can be further boosted by applying higher pressures or strain engineering in the heterostructure films. Despite much recent effort, the highest reported T c of the infinite-layer nickelates remains lower than 15 K. Here, the authors apply pressure to Pr 0.82 Sr 0.18 NiO 2 thin films and observe a monotonic increase of T c to 31 K at 12.1 GPa, an increase that does not level off with increasing pressure.

In addition to protein-coding genes, the human genome makes a large amount of noncoding RNAs, including microRNAs and long noncoding RNAs (lncRNAs). Both microRNAs and lncRNAs have been shown to have a critical role in the regulation of cellular processes such as cell growth and apoptosis, as well as cancer progression and metastasis. Although it is well known that microRNAs can target a large number of protein-coding genes, little is known whether microRNAs can also target lncRNAs. In the present study, we determine whether miR-21 can regulate lncRNA expression. Using the lncRNA RT-PCR (reverse transcription-polymerase chain reaction) array carrying 83 human disease-related lncRNAs, we show that miR-21 is capable of suppressing the lncRNA growth arrest-specific 5 (GAS5). This negative correlation between miR-21 and GAS5 is also seen in breast tumor specimens. Of interest, GAS5 can also repress miR-21 expression. Whereas ectopic expression of GAS5 suppresses, GAS5–siRNA increases miR-21 expression. Importantly, there is a putative miR-21-binding site in exon 4 of GAS5; deletion of the miR-21-binding site abolishes this activity. Experiments with in vitro cell culture and xenograft mouse model suggest that GAS5 functions as a tumor suppressor. We further show that the biotin-labeled GAS5–RNA probe is able to pull down the key component (AGO2) of the RNA-induced silencing complex (RISC) and we subsequently identify miR-21 in this GAS5–RISC complex, implying that miR-21 and GAS5 may regulate each other in a way similar to the microRNA-mediated silencing of target mRNAs. Together, these results suggest that miR-21 targets not only tumor-suppressive protein-coding genes but also lncRNA GAS5.

Osteoporosis is a highly prevalent disease with distinct sex pattern. We aimed to estimate the sex specific incidence, prevalence, and disability-adjusted life (DALYs) years of osteoporosis between 1990 and 2019, with additional predictions from 2020 to 2034. We collected osteoporosis disease burden data from the Global Burden of Disease study covering the years 1990 through 2019 in 204 countries and territories. The data included information on the number of incident cases of osteoporosis, DALYs, age-standardized incidence rates (ASIR), age-standardized prevalence rates (ASPR) and age-standardized DALYs rates. Additionally, we performed an age-period-cohort analysis to forecast the burden of osteoporosis. The global number of incidence cases of osteoporosis, in 2019, reached 41.5 million cases. From 1990 to 2019, the low-middle socio-demographic index (SDI) region had the highest estimated annual percentage change in the world. Compared to males, female's ASIR and ASPR were all about 1.5 times higher than males for the same years in the same SDI regions. The projected global total number of incidence cases for osteoporosis between 2030 and 2034 is estimated to reach 263.2 million (154.4 million for females and 108.8 for males). Additionally, the burden in terms of DALYs is predicted to be 128.7 million (with 78.4 million for females and 50.3 million for males). The global burden of osteoporosis is still increasing, mainly observed in high SDI countries. Females bear a burden 1.5 times higher than males in terms of incidence and DALYs. Steps should be taken to reduce the osteoporosis burden, especially in high SDI countries.

In this paper, the damage mechanism of sandstone under the action of water and force is clarified by analyzing different creep stages of rock, and the corresponding damage variables are put forward. The effects of accelerated creep and initial water content on the model are fully considered by improving the Model. Based on the relationship between model parameters and initial water content as well as between model parameters and stress, a new method for determining creep parameters is proposed, and a relatively perfect nonlinear creep constitutive model of rock under the action of water and force is established. Taking sandstone as the research object, data were obtained through uniaxial creep tests under different initial water contents, and verification was conducted combined with FLAC 3D numerical simulation. Compared with the experimental results and numerical results, the model established in this paper can better reflect the deterioration characteristics of sandstone under water–rock coupling. Finally, the damage characteristics of rock with variable water content are briefly analyzed, and the superiority of the nonlinear creep constitutive model of rock with variable water content under the action of water and force is proposed. The results show that the model can well reflect the deterioration characteristics of sandstone under water–rock coupling.

The authors assess the ability of 18 Earth system models to simulate the land and ocean carbon cycle for the present climate. These models will be used in the next Intergovernmental Panel on Climate Change (IPCC) Fifth Assessment Report (AR5) for climate projections, and such evaluation allows identification of the strengths and weaknesses of individual coupled carbon–climate models as well as identification of systematic biases of the models. Results show that models correctly reproduce the main climatic variables controlling the spatial and temporal characteristics of the carbon cycle. The seasonal evolution of the variables under examination is well captured. However, weaknesses appear when reproducing specific fields: in particular, considering the land carbon cycle, a general overestimation of photosynthesis and leaf area index is found for most of the models, while the ocean evaluation shows that quite a few models underestimate the primary production. The authors also propose climate and carbon cycle performance metrics in order to assess whether there is a set of consistently better models for reproducing the carbon cycle. Averaged seasonal cycles and probability density functions (PDFs) calculated from model simulations are compared with the corresponding seasonal cycles and PDFs from different observed datasets. Although the metrics used in this study allow identification of somemodels as better or worse than the average, the ranking of this study is partially subjective because of the choice of the variables under examination and also can be sensitive to the choice of reference data. In addition, it was found that the model performances show significant regional variations.

It is generally believed that long-duration gamma-ray bursts (GRBs) originate from the core collapse of rapidly spinning massive stars, and at least some of them are powered by hyperaccreting black holes (BHs). However, definite proofs about the progenitor and central engine of these GRBs have not been directly observed in the past. Here, we report the existence of a quasiperiodic oscillation (QPO) signature with periodic frequency ∼0.02 Hz in the early X-ray afterglow phase of GRB 220711B. Such a low-frequency QPO likely signals the precession of a relativistic jet launched from a GRB hyperaccreting BH central engine. The energy injection signature from the late X-ray observations (from 5 × 102 s ∼ 1 × 104 s) is consistent with the precession hypothesis. The prompt gamma-ray light curve does not show any QPO signature, suggesting that the X-ray flaring emission in the early afterglow phase and prompt emission likely originate from different accretion processess, indicating that the progenitor stars of GRBs have a core-envelope structure with a stratified angular momentum distribution and the late-time accretion disk likely has a misalignment with respect to the rotation axis of the BH. Such a misalignment is not expected in a canonical collapsar model. As a result, the QPO signature in GRB 220711B may reveal a new formation channel of long GRBs, possibly a stellar-merger-induced core collapse, with the orbital angular momentum of the binary misaligned with the spin axis of the collapsing star.

Aim: Our scientific understanding of the extent and distribution of mangrove forests of the world is inadequate. The available global mangrove databases, compiled using disparate geospatial data sources and national statistics, need to be improved. Here, we mapped the status and distributions of global mangroves using recently available Global Land Survey (GLS) data and the Landsat archive. Methods: We interpreted approximately 1000 Landsat scenes using hybrid supervised and unsupervised digital image classification techniques. Each image was normalized for variation in solar angle and earth-sun distance by converting the digital number values to the top-of-the-atmosphere reflectance. Ground truth data and existing maps and databases were used to select training samples and also for iterative labelling. Results were validated using existing GIS data and the published literature to map ' true mangroves'. Results: The total area of mangroves in the year 2000 was 137,760 km² in 118 countries and territories in the tropical and subtropical regions of the world. Approximately 75% of world's mangroves are found in just 15 countries, and only 6.9% are protected under the existing protected areas network (IUCN I-IV). Our study confirms earlier findings that the biogeographic distribution of mangroves is generally confined to the tropical and subtropical regions and the largest percentage of mangroves is found between 5° N and 5° S latitude. Main conclusions: We report that the remaining area of mangrove forest in the world is less than previously thought. Our estimate is 12.3% smaller than the most recent estimate by the Food and Agriculture Organization (FAO) of the United Nations. We present the most comprehensive, globally consistent and highest resolution (30 m) global mangrove database ever created. We developed and used better mapping techniques and data sources and mapped mangroves with better spatial and thematic details than previous studies.