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Background Due to cowpea ability to fix nitrogen in poor soils and relative tolerance to drought and salt stresses, efforts have been directed to identifying genes and pathways that confer stress tolerance in this species. Real-time quantitative PCR (qPCR) has been widely used as the most reliable method to measure gene expression, due to its high accuracy and specificity. In the present study, nine candidate reference genes were rigorously tested for their application in normalization of qPCR data onto roots of four distinct cowpea accessions under two abiotic stresses: root dehydration and salt (NaCl, 100 mM). In addition, the regulation of four target transcripts, under the same referred conditions was also scrutinized. Results geNorm, NormFinder, BestKeeper, and ΔCt method results indicated a set of three statistically validated RGs for each stress condition: (I) root dehydration (actin, ubiquitin-conjugating enzyme E2 variant 1D, and a Phaseolus vulgaris unknown gene— UNK ), and (II) salt (ubiquitin-conjugating enzyme E2 variant 1D, F-box protein, and UNK ). The expression profile of the target transcripts suggests that flavonoids are important players in the cowpea response to the abiotic stresses analyzed, since chalcone isomerase and chalcone synthase were up-regulated in the tolerant and sensitive accessions. A lipid transfer protein also participates in the cowpea tolerance mechanisms to root dehydration and salt stress. The referred transcript was up-regulated in the two tolerant accessions and presented no differential expression in the sensitive counterparts. Chitinase B, in turn, generally related to plant defense, was an important target transcript under salt stress, being up-regulated at the tolerant, and down-regulated in the sensitive accession. Conclusions Reference genes suitable for qPCR analyses in cowpea under root dehydration and salt stress were identified. This action will lead to a more accurate and reliable analysis of gene expression on this species. Additionally, the results obtained in this study may guide future research on gene expression in cowpea under other abiotic stress types that impose osmotic imbalance. The target genes analyzed, in turn, deserve functional evaluation due to their transcriptional regulation under stresses and biotechnological potential.

Agroforestry systems (AFSs) exhibit varied composition and dynamics as intrinsic characteristics of their specificities. In this context, a review of the adoption, composition, and dynamics of AFSs in the Amazon biome was conducted to identify the origin, institutions, and researchers of published studies with results on this scientific topic, focused on trends and characteristics of AFSs diversity in the Amazon. The methodology adopted was a scoping review, based on searches in the Scopus and Web of Science databases, using specific keywords to ensure that the articles addressed topics related to the adoption, composition, and dynamics of AFSs in the Amazon. Following the selection of subtopics, 66 articles were selected and analyzed. The analysis revealed that research on AFSs in the Amazon highlights interactions among traditional knowledge, innovations, and sustainability. The analysis of research published between 1996 and 2023 indicated growth in studies with an interdisciplinary focus, primarily from Brazil. However, internationalization, collaborative networks, and funding factors contribute to the prominence of foreign institutions. Research studies often address topics such as species diversity, agrobiodiversity, and tree growth in agroforestry intercrops. In this context, homegarden agroforestry (HAF) emerges as one of the main subjects of study, encompassing multifunctional environments, richness diversity, and ongoing experimentation with plant species. The choice of species for AFSs is influenced by factors such as labor, personal preferences, and market demands, although loggers and commercial forestry systems tend to have lower diversity, contrasting with HAF. AFSs implementation methods vary according to financing, management, and the farmer’s education and gender. Environmental conservation, food security, ecosystem services, and production flexibility are highlighted as benefits of AFSs, while challenges include technical and economic limitations. This research highlights the strengthening and consolidation of AFSs by addressing scientific gaps and demonstrating the need for studies on the adoption, consolidation, and management of these systems, as well as the relationship between diversity and yield. Future research should be concentrated on deepening studies on the relationship between diversity and yield in AFSs, as well as on management strategies that support the consolidation of these systems in the Amazon biome, integrating innovation, public policy support, and traditional knowledge of farmers.

New-generation sequencing (NGS) techniques have brought the opportunity for genomic monitoring of several microorganisms potentially relevant to public health. The establishment of different methods with different mechanisms provides a wide choice, taking into account several aspects. With that in mind, the present aim of the study was to compare basic genomic sequencing metrics that could potentially impact genotyping by nanopores from Oxford Nanopore Technologies and by synthesis from Illumina in clinical samples positive for Chikungunya (CHIKV). Among the metrics studied, running time, read production, and Q score were better represented in Illumina sequencing, while the MinIOn platform showed better response time and greater diversity of generated files. That said, it was possible to establish differences between the studied metrics in addition to verifying that the distinctions in the methods did not impact the identification of the CHIKV virus genotype.

This study compared the behavior of the biomass in two fixed-film anaerobic reactors operated under equivalent organic loading rates but at different temperatures, i.e., 30 °C (RMM) and 55 °C (RMT). The reactors were fed with sugarcane vinasse and molasses (both fermented) in a simulation of sequential periods of season and off-season. The dynamics of biomass growth and retention, as well as the microbial composition, were assessed throughout 171 days of continuous operation, coupled with an additional 10-day test assessing the microbial activity in the bed region. Despite the different inoculum sources used (mesophilic granular vs. thermophilic flocculent sludge types), the biomass growth yield was identical (0.036–0.038 g VSS g−1COD) in both systems. The retention rates (higher in RMT) were regulated according to the initial amount of biomass provided in the inoculation, resulting in similar amounts of total retained biomass (46.8 vs. 43.3 g VSS in RMT and RMM) and biomass distribution patterns (30–35% in the feeding zone) at the end of the operation. Meanwhile, hydrogenotrophic methanogenesis mediated by Methanothermobacter coupled to syntrophic acetate oxidation prevailed in RMT, while the Methanosaeta-mediated acetoclastic pathway occurred in RMM. The results show that different anaerobic consortia can behave similarly in quantitative terms when subjected to equivalent organic loads, regardless of the prevailing methane-producing pathway. The community grows and reaches a balance (or a given cell activity level) defined by the amount of substrate available for conversion. In other words, while the metabolic pathway may differ, the endpoint (the amount of biomass) remains the same if operational stability is maintained.

The study aimed to investigate the chemical composition and the anti-inflammatory activity of the hydroethanolic rhizomes, stems, and leaf extracts of Renealmia petasites using in vitro and in vivo assays. The chemical composition of the extracts was characterized in a linear iron trap mass spectrometer. Total phenolic, flavonoid, and tannin content were determined by spectrophotometry analyses. In vitro anti-inflammatory activity was investigated in lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O 2 − ), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6). In vivo effects were determined using the air pouch model in which were inoculated carrageenan and thereafter treated with 50 mg/kg of the hydroethanolic extracts of R. petasites . After 4 and 24 h, the cellular influx, protein exudation, cytokines, and nitric oxide were evaluated. Eight compounds were tentatively identified in the R. petasites extracts, suggesting five diarylheptanoids, one flavonoid, and two fatty alcohols. The in vitro results showed that the extracts were capable of blocking free radicals and/or inhibiting their intracellular actions by inhibiting the production of important mediators of the inflammatory process, such as NO, O 2 − , TNF-α, and IL-6. In vivo, R. petasites significantly decrease the influx of leukocytes, mainly neutrophils, protein exudation, NO, TNF-α, and IL-6 concentration in the air pouch model. The results evidenced that R. petasites can be considered a promising alternative therapy for the treatment and management of osteoarthritis and other inflammatory diseases.

Biotech Network (Rede Biotech in Portuguese) is a crossdisciplinary initiative launched at the Federal University of Santa Catarina (UFSC) to foster innovative biotechnological solutions aligned with Brazil’s scientific, environmental, and societal challenges. Here, we highlight the structure, mission, and pioneering research efforts of the network, offering a model for integrated and sustainable biotechnology development in emerging economies. Biotech Network (Rede Biotech in Portuguese) is a crossdisciplinary initiative launched at the Federal University of Santa Catarina (UFSC) to foster innovative biotechnological solutions aligned with Brazil’s scientific, environmental, and societal challenges. Here, we highlight the structure, mission, and pioneering research efforts of the network, offering a model for integrated and sustainable biotechnology development in emerging economies.

Plasmodial infection induces systemic inflammation with great potential to contribute to the development of severe illness and lethality. It is known that the hippocampus and cortex both play a pivotal role in memory processes and are affected by the neuroinflammation associated with cerebral malaria that causes long-lasting cognitive and behavioral sequelae. Since these sequelae are also associated with the non-severe form of malaria, it is important to correlate brain morphology, particularly glial cell involvement, and neuroimmune and neuroinflammatory features with memory acquisition and consolidation processes, in this clinical presentation of malaria, the most frequent worldwide. Here, we aimed to investigate cellular and molecular neuroimmune aspects of non-severe experimental malaria-associated cognitive dysfunction. Female C57BL/6 mice were infected with Plasmodium berghei ANKA and treated with chloroquine before any clinical signs of cerebral malaria emerged. No histopathological alteration, in hematoxylin-eosin staining, or axonal damage, in Bielschowsky’s silver-impregnated brain sections, was observed. However, morphological alterations in GFAP+ and Iba-1+ cells suggest that: i) astrocytes in the hippocampal dentate gyrus and the cornu Ammonis 1 regions and ii) microglia in the cornu Ammonis 1 region are responding to infection. Curiously, the effect persisted only in Iba-1+ cells up to 22 days post-infection. An increase in pro-inflammatory cytokines levels and expression was observed, in both the prefrontal cortex and the hippocampus. Also, serum and spleen cytokine levels were increased at 4 days post-infection. At 22 days post-infection, infected and treated mice showed an increase in serum cytokine levels that had homeostatic levels at 155 days post-infection. This dynamic points to both an immune stimulus persistence and a cytokine autoregulation post-infection. Infected mice exhibited acquisition and consolidation memory deficits in behavioral tests early after treatment (22 days post-infection). In conclusion, in a context of sustained systemic inflammation, mild neuroinflammatory alterations of glial cells may be involved in cognitive sequelae following a single episode of non-severe experimental malaria.