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The blend sign (BS) is a known predictor of hemorrhage expansion. However, its prognostic value remains unclear. Therefore, we aimed to compare functional and mortality outcomes between intracerebral hemorrhage (ICH) patients with and without BS. We searched PubMed, EMBASE, Web of Science, and Cochrane Library for studies comparing patients with and without BS who underwent either conservative treatment or surgical evacuation for spontaneous ICH. Outcomes were functional status, hemorrhage expansion, mortality, and initial hematoma volume. Statistical analysis was performed using R software (version 4.4.0), and heterogeneity was assessed with I2 statistics. We included 13 studies with a total of 8685 patients, of whom 5131 (59.08 %) were male. The mean age was 64.64 ± 4.92 years, and the follow-up period ranged from 30 to 360 days. Poor neurological outcomes (OR 1.71, 95 % CI: 1.23─2.37, p < 0.01, I2 = 84.2 %) and hemorrhage expansion (OR 2.11, 95 % CI: 1.36─3.26, p < 0.01, I2 = 42 %) were significantly higher in patients with BS. However, the overall mortality rate (OR 1.56, 95 % CI: 0.95─2.58, p = 0.08, I2 = 0 %) was not statistically different between groups. Furthermore, no significant differences were found in initial hematoma volume (MD 1.26 mL, 95 % CI: −9.94–12.46, p = 0.83, I2 = 92 %). Patients with blend sign are associated with higher rates of poor neurological outcomes and hemorrhage expansion. Therefore, BS is a clear and easily identifiable finding that, based on our results, can improve patient management and treatment selection. •This meta-analysis compared outcomes of patients with and without the blend sign.•The blend sign was linked to significantly worse functional outcomes in patients.•No significant difference in mortality rates was found between the two groups.•Hematoma expansion occurred more often in patients with the blend sign.•The blend sign marks increased severity in spontaneous intraparenchymal hemorrhage.

Background: The Pilates Method is a program of physical and mental training that involves the whole body, aiming at the gain of muscular strength, mainly of the central region, formed by the abdominal muscles, the spine and the pelvic floor muscles (PFM). The PFM responds to sexual stimulation, with increased local blood circulation and involuntary contractions during orgasm. The training of this musculature assists in the female sexual function. Objective: To evaluate the influence of Pilates Mat associated with perineal contraction in the female sexual response. Method: Uncontrolled clinical trial in which participated twelve women between 20 and 50 years old, who performed Mat Pilates classes associated with perineal exercises, three times a week. The sexual response was evaluated through the Female Sexual Function Index (FSFI) and the contraction capacity of PFM through the Functional Evaluation of the Pelvic Floor (FEPF) before and after 21 sessions. Results: Given the proposed treatment, the FSFI responded positively (0.0099), with an improvement in the median and interquartile range that initially was 25.6 ± 5.4 and increased to 28.4 ± 1.2. The Functional Evaluation of the Pelvic Floor (FEPF) also achieved significant improvement (p= 0.0077), increased from the initial median and interquartile range of 2 ± 1.5 to 4 ± 1. Conclusion: Mat Pilates provides encouraging results, and its use is relevant to promote improved sexual response and strength gain of the pelvic floor muscle.

Introduction Meckel-Gruber Syndrome (MKS) is an autosomal recessive genetic disorder, notable for its triad of occipital encephalocele, polycystic renal dysplasia, and postaxial polydactyly. Identified by Johann Friederich Meckel in 1822, MKS is categorized as a ciliopathy due to gene mutations. Diagnosis is confirmed by the presence of at least two key features. The condition is incompatible with life, leading to death in the womb or shortly after birth. Recent studies have largely focused on the genetic aspects of MKS, with limited information regarding the impact of neurosurgical approaches, particularly in treating encephaloceles. Methods A systematic review was performed according to the PRISMA statement. The PubMed, Embase, and Web of Science databases were consulted for data screening and extraction, which was conducted by two independent reviewers. The search strategy aimed to encompass studies documenting cases of MKS with published reports of encephalocele excisions, and the search strings for all databases were: Meckel-Gruber syndrome OR Meckel Gruber syndrome OR Meckel-gruber OR Meckel Gruber. Results The study included 10 newborns with MKS associated with occipital encephalocele or meningocele, all of whom underwent surgical repair of the occipital sac. The mean gestational age at birth was 36 (± 2) weeks. The mean of birth weight was 3.14 (± 0.85) kilograms. The average head circumference at birth was 33.82 cm (± 2.17). The mean diameter of the encephalocele/meningocele was 5.91 (± 1.02) cm. Other common central nervous system abnormalities included hydrocephalus, Dandy-Walker malformation, and agenesis of the corpus callosum. 40% required shunting for hydrocephalus. Surgery to remove the occipital sac occurred at a median age of 2.5 days (1.5–6.5). The most common post-surgical complication was the need for mechanical ventilation. The most common cause of death was pneumonia and the median age at death was 6.66 (0.03–18) months. Conclusion Our findings suggest that neurosurgical intervention, especially for managing encephaloceles, may offer some improvement in survival, albeit within a context of generally poor prognosis. However, these results should be interpreted with caution.

This study presents the first description of Bovine herpesvirus 6 (BoHV-6) that was isolated from buffaloes of Amazon region in Brazil. Phylogenetic analysis showed that the BoHV-6 Brazilian strains clustered with the sequence of BoHV-6 from elsewhere available at the GenBank. It was observed in some buffaloes with lymphoproliferative disease in one herd, thus the animals were also tested for Bovine leukemia virus (BLV), which has been associated to lymphoma in bovines. All animals were negative to BLV. These results indicate that BoHV-6 is present in buffaloes in Brazil, but the importance and impact of this infection and its association with any illness is still undefined.

Niobium weld seams performed by laser technology, have received nitrogen plasma based ion implantation (PBII) to nitride formation on the material surface layers. The treatment was performed in temperature range of 1000-1200°C for 1h. Results show a significant change of the chemical composition in the material surface, with the presence of niobium nitrides in this region. In general, nitrides of refractory materials have desirable properties for applications involving high temperature working such as: thermal stability, high melting point and good oxidation resistance. Due to the substrate heating during the heat treatment, it was observed microstructural changes of the treated material with homogeneity of hardness values in different regions: weld bead, heat affected zone (HAZ) and base material.

Silicon (Si) supplementation is well-known for enhancing plant resistance to insect pests, however, only recently studies revealed that Si accumulation in the plant not only confers a mechanical barrier to insect feeding, but also primes jasmonic acid-dependent defenses. Here, we examined whether Si supplementation alters wheat volatile emissions that influence the bird cherry-oat aphid (Rhopalosiphum padi) olfactory preference and the aphid parasitoid Lysiphlebus testaceipes. Even though Si accumulation in wheat did not impact aphid performance, we found that R. padi preferred constitutive volatiles from-Si wheat over those emitted by +Si wheat plants. In Y-tube olfactometer bioassays, the parasitoid was attracted to volatiles from +Si uninfested wheat, but not to those from-Si uninfested wheat. +Si and-Si aphid-infested plants released equally attractive blends to the aphid parasitoid; however, wasps were unable to distinguish +Si uninfested plant odors from those of aphid-infested treatments. GC-MS analyses revealed that +Si uninfested wheat plants emitted increased amounts of a single compound, geranyl acetone, compared to -Si uninfested wheat, but similar to those emitted by aphid-infested treatments. By contrast, Si supplementation in wheat did not alter composition of aphid-induced plant volatiles. Our results show that changes in wheat volatile blend induced by Si accumulation mediate the non-preference behavior of the bird cherry-oat aphid and the attraction of its parasitoid L. testaceipes. Conversely to the literature, Si supplementation by itself seems to work as an elicitor of induced defenses in wheat, and not as a priming agent.

The numerous health benefits associated with the use of plants in traditional medicine can be linked to secondary metabolites in the products obtained through the hydrodistillation process, such as hydrolates and essential oils (EOs). This study aimed to assess the chemical profile and the antioxidant, antimicrobial, cytotoxic, and anti-inflammatory activities of two hydrodistillation products obtained from four plants belonging to two distinct families: chamomile (Matricaria chamomilla), purple coneflower (Echinacea purpurea), conehead thyme (Thymbra capitata), and basil (Ocimum basilicum). The EOs were found to be more effective than hydrolates in inhibiting microorganisms’ growth, registering MIC values equal to or higher than 50% (v/v), except for T. capitata. Only T. capitata demonstrated the ability to reduce NO levels with both hydrolate and EO. Moreover, it inhibited the cell growth of RAW 264.7, 3T3, and HaCaT lines at the tested concentrations. In contrast, O. basilicum EO did not affect the cell proliferation of the tested cell lines in concentrations below 0.063% (v/v) and showed a significant reduction in the macrophage NO production at all concentrations. Thus, EOs showed a superior biological activity than hydrolates. In particular, O. basilicum EO was found to be a promising candidate for therapeutic applications on the skin.

Alzheimer’s and Parkinson’s diseases are considered the most common neurodegenerative disorders, representing a major focus of neuroscience research to understanding the cellular alterations and pathophysiological mechanisms involved. Several natural products, including flavonoids, are considered able to cross the blood-brain barrier and are known for their central nervous system-related activity. Therefore, studies are being conducted with these chemical constituents to analyze their activities in slowing down the progression of neurodegenerative diseases. The present systematic review summarizes the pharmacological effects of flavonoids in animal models for Alzheimer’s and Parkinson’s diseases. A PRISMA model for systematic review was utilized for this search. The research was conducted in the following databases: PubMed, Web of Science, BIREME, and Science Direct. Based on the inclusion criteria, 31 articles were selected and discussed in this review. The studies listed revealed that the main targets of action for Alzheimer’s disease therapy were reduction of reactive oxygen species and amyloid beta-protein production, while for Parkinson’s disease reduction of the cellular oxidative potential and the activation of mechanisms of neuronal death. Results showed that a variety of flavonoids is being studied and can be promising for the development of new drugs to treat neurodegenerative diseases. Moreover, it was possible to verify that there is a lack of translational research and clinical evidence of these promising compounds.

Zika virus (ZIKV) is an arbovirus from the Flaviviridae family and Flavivirus genus. Neurological events have been associated with ZIKV-infected individuals, such as Guillain-Barré syndrome, an autoimmune acute neuropathy that causes nerve demyelination and can induce paralysis. With the increase of ZIKV infection incidence in 2015, malformation and microcephaly cases in newborns have grown considerably, which suggested congenital transmission. Therefore, the development of an effective vaccine against ZIKV became an urgent need. Live attenuated vaccines present some theoretical risks for administration in pregnant women. Thus, we developed an in silico multiepitope vaccine against ZIKV. All structural and non-structural proteins were investigated using immunoinformatics tools designed for the prediction of CD4 + and CD8 + T cell epitopes. We selected 13 CD8 + and 12 CD4 + T cell epitopes considering parameters such as binding affinity to HLA class I and II molecules, promiscuity based on the number of different HLA alleles that bind to the epitopes, and immunogenicity. ZIKV Envelope protein domain III (EDIII) was added to the vaccine construct, creating a hybrid protein domain-multiepitope vaccine. Three high scoring continuous and two discontinuous B cell epitopes were found in EDIII. Aiming to increase the candidate vaccine antigenicity even further, we tested secondary and tertiary structures and physicochemical parameters of the vaccine conjugated to four different protein adjuvants: flagellin, 50S ribosomal protein L7/L12, heparin-binding hemagglutinin, or RS09 synthetic peptide. The addition of the flagellin adjuvant increased the vaccine's predicted antigenicity. In silico predictions revealed that the protein is a probable antigen, non-allergenic and predicted to be stable. The vaccine’s average population coverage is estimated to be 87.86%, which indicates it can be administered worldwide. Peripheral Blood Mononuclear Cells (PBMC) of individuals with previous ZIKV infection were tested for cytokine production in response to the pool of CD4 and CD8 ZIKV peptide selected. CD4 + and CD8 + T cells showed significant production of IFN-γ upon stimulation and IL-2 production was also detected by CD8 + T cells, which indicated the potential of our peptides to be recognized by specific T cells and induce immune response. In conclusion, we developed an in silico universal vaccine predicted to induce broad and high-coverage cellular and humoral immune responses against ZIKV, which can be a good candidate for posterior in vivo validation.

Arrowroot starch (AA)-based films incorporated with a carnauba wax nanoemulsion (CWN), cellulose nanocrystals (CNCs), and essential oils (EOs) from Mentha spicata (MEO) and Cymbopogon martinii (CEO) were produced using the casting technique and then characterized in terms of their water barrier, tensile, thermal, optical, and microstructural properties and in vitro antifungal activity against Rhizopus stolonifer and Botrytis cinerea. Whereas the incorporation of CNCs decreased the moisture content and water vapor permeability of the AA/CWN/CNC film, the additional incorporation of either EO decreased the transparency and affected the microstructure of the AA/CWN/CNC/EO nanocomposites. MEO and CEO incorporation improved the thermal stability of the films and provided excellent protection against fruit-spoiling fungi. Because of their excellent barrier properties against fungal growth, water vapor permeability, and ultraviolet and visible light, these AA/CWN/CNC/EO films have promising potential for application as active food packaging or coating materials. Graphic abstract