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There is great interest for NPs manufacturing by environmentally friendly and economic manner. The aqueous leaves extract of Thuja orientalis was used to synthesized silica nanoparticles (SiO2NPs) by using two green methods, the first one is the magnetic stirrer method and the second method by using the cold plasma. The XRD pattern for both samples annealed at Ta=600˚C for 1h, while they showed the characteristic of Bragg peaks for poly phases at (001), (010), (10-1), (1-10), (0-12) and (002) for SiO2 triclinic (anorthic). The average crystalline size was calculated by using Scherer’s formula, which was 11.1868 nm in magnetic stirrer method, while the cold plasma method showed amorphous structure. The morphology analysis using atomic force microscopy showed that the grain size was 33.94 and 18.37 nm for magnetic stirrer and cold plasma methods respectively. Fourier Transform Infrared Spectroscopy (FTIR) analysis indicates hydrophilic functional groups in the capping matrix, which can improve the stability of silica NPs. The biofilm inhibition of silica NPs were investigated for two genus of bacteria Staphylococcus aureus and Escherichia coli, the green silica NPs that synthesized by using cold plasma method showed the highest inhibition effect on S. aureus and E. coli respectively.

The present study was aimed to biosynthesis of silver nanoparticles by using rhaminolipid produced from local isolate Pseudomonas aeruginosa as reducing and stabilizing agent. Silver nanoparticles (AgNPs) synthesized by green method have shown several applications such as biomedical, anticancer, bio sensing, catalysis etc. Characterization study of purified bioemulsifier using thin layer chromatography (TLC) was demonstrated that the biosurfactant contains mono, and di- rhamnolipid with Rf values of 0.86 and 0.36 respectively. Optimization results of biosynthesis silver nanoparticles were revealed that an increasing in intensity of Surface Plasmon Resonance (SPR) bands of nanoparticles with shifting at wavelength (400 nm). Also optimum synthesis of AgNPs was at pH 5, Temperature 40℃, reaction time 5 minutes with concentration of rhaminolipid as reducing agents (2×10-3w/v) and Silver ion concentration (6×10-3 mol/L). The result of X-ray diffraction was indicated that the size of silver nanoparticles observed was 38 nm and show relatively stable peak at -23.2 mV. Finally, the minimum inhibitory concentration of Ag NPs against human pathogenic bacteria obtained at concentration (1mg/ml) for both gram negative and gram-positive bacteria. The results of antiinflammatory effects of Ag NPs obviously, cleared that the infection of test animals treated with AgNPs were completely healed after 6 days of treatment, while the animals treated with fucidin (as control) not exhibited any healing in the infection.