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The present study was aimed to biosynthesis of silver nanoparticles by using rhaminolipid produced from local isolate Pseudomonas aeruginosa as reducing and stabilizing agent. Silver nanoparticles (AgNPs) synthesized by green method have shown several applications such as biomedical, anticancer, bio sensing, catalysis etc. Characterization study of purified bioemulsifier using thin layer chromatography (TLC) was demonstrated that the biosurfactant contains mono, and di- rhamnolipid with Rf values of 0.86 and 0.36 respectively. Optimization results of biosynthesis silver nanoparticles were revealed that an increasing in intensity of Surface Plasmon Resonance (SPR) bands of nanoparticles with shifting at wavelength (400 nm). Also optimum synthesis of AgNPs was at pH 5, Temperature 40℃, reaction time 5 minutes with concentration of rhaminolipid as reducing agents (2×10-3w/v) and Silver ion concentration (6×10-3 mol/L). The result of X-ray diffraction was indicated that the size of silver nanoparticles observed was 38 nm and show relatively stable peak at -23.2 mV. Finally, the minimum inhibitory concentration of Ag NPs against human pathogenic bacteria obtained at concentration (1mg/ml) for both gram negative and gram-positive bacteria. The results of antiinflammatory effects of Ag NPs obviously, cleared that the infection of test animals treated with AgNPs were completely healed after 6 days of treatment, while the animals treated with fucidin (as control) not exhibited any healing in the infection.

This study was aimed to produce silver nanoparticles by fruits extract of Juniperus phoenicea and comparison between the ethanolic extract and nano extract through the antioxidant, antibacterial, anti-parasite and cytotoxicity against prostate cancer. The synthesis of silver nanoparticles was shown by many characterizing techniques: UV, FTIR, XRD and SEM. Nano extract exhibited a higher antibacterial, antioxidant, antiparasite compared to ethanolic extract and higher cytotoxicity activity. The nano extract exhibited higher antibacterial activity compared with antibiotics. Finally, we study the toxicity of J. phoenicea by Inhibition of RBC hemolysis by H2O2, the results exhibited the highest inhibition activity of J. phoenicea against H2O2. The nanoextract of J. phoenicea can be used effectively in the production of potential antioxidant, antiparasite antimicrobial and anticancer.

This advanced scientific study by Fatima Attaher Abdusslam focuses on developing modern organic formulations capable of fighting cancer without inducing cytotoxicity to healthy cells. The research addresses one of the key challenges in oncology-the high toxicity of conventional chemotherapy-and seeks to design selective, biocompatible organic compounds based on principles of green chemistry and molecular targeting. Employing spectroscopic techniques such as mass spectrometry, infrared (IR), and nuclear magnetic resonance (NMR), the author characterized the synthesized compounds and evaluated their bioactivity through in-vitro assays on various cancer cell lines. The findings reveal several novel organic molecules that exhibit strong anticancer activity with minimal toxicity, particularly against liver and lung cancer cells, while selectively triggering apoptosis in malignant cells. Some compounds also demonstrated antioxidant properties, reducing oxidative stress and protecting non-cancerous tissues during treatment. The study highlights the potential of these new agents as non-toxic, targeted anticancer therapies, emphasizing the shift from destructive chemotherapy to regenerative molecular modulation. Abdusslam concludes that continued preclinical development of these compounds could lead to a new generation of safe, sustainable, and precision-driven cancer treatments, representing a major step toward intelligent organic medicines that reprogram rather than destroy cells. Abstract Written by Dar AlMandumh, 2025, Using AI