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Appropriate lifestyle pattern and behavior interventions are crucial to weight loss success but maintaining is extremely challenging. The current study aimed to evaluate the effect of mixing some spices (cinnamon, cardamom, turmeric, ginger, and clove) on biochemical parameters and body weight gain in obese rats. This work was carried out on (40) Adult male Wister albino rats (weighing 120 ± 20 g) randomly classified into five groups. control (c-), group (2) was fed with High Fat Diet (c+). group (3,4, and 5) fed with HFD + different mixing of spices (cinnamon, cardamom, turmeric, ginger, and clove) throughout the experimental period of 8 weeks. Chemical composition, Mineral's content, fatty acid, phenolic compounds, biochemical signs, Seram glucose level, body weight gain, liver & kidney function and histopathological examinations were determined. The results showed that there was a significant difference in Chemical composition and fatty acids content between the three tested samples. according to Biochemical Parameters treated groups with the three mixes shows a significant decrease in Cholesterol, Triglyceride, and LDL-c, and a significant increase in HDL-c. especially the group fed on mix 1 reported a great improvement in Cholesterol, Triglyceride, HDL-c, and LDL-c, compared with groups fed on mix 2 or mix 3. the three treated groups showed a significant improve in Seram glucose level. and a significant decrease in (BWG) especially the group fed on mix 3. it could be noticed that the mixing several medical spices together will provides more significant effect in treating obesity, hyperlipidemia, and high blood glucose levels especially mix (1) was the most effective mix.