Explore the vast range of titles available.

Bone Research is supported by West China School of Stomatology, Sichuan University State Key Laboratory of Oral Diseases, Sichuan University International Chinese Musculoskeletal Research Society （ICMRS） Type of manuscript accepted Review articles provide a comprehensive review of the state of the art on topics selected by the Editor-in-Chief. Reviews should not be submitted without contacting the Editorial Office. A comprehensive review or full review should be thoroughly cover the most recent progress in bone related field.

Bone Research is supported by West China School of Stomatology, Sichuan University State Key Laboratory of Oral Diseases, Sichuan University International Chinese Musculoskeletal Research Society （ICMRS） Type of manuscript accepted Review articles provide a comprehensive review of the state of the art on topics selected by the Editor-in-Chief. Reviews should not be submitted without contacting the Editorial Office. The word limit is 10 000 words （not including the title, author affiliations, abstracts, or references） and 200 references.

Bone Research is supported by West China School of Stomatology, Sichuan University State Key Laboratory of Oral Diseases, Sichuan University International Chinese Musculoskeletal Research Society （ICMRS） Type of manuscript accepted Review articles provide a comprehensive review of the state of the art on topics selected by the Editor-in-Chief. Reviews should not be submitted without contacting the Editorial Office. The word limit is 10 000 words （not including the title, author affiliations, abstracts, or references） and 200 references.

Bone Research is supported by West China School of Stomatology, Sichuan University State Key Laboratory of Oral Diseases, Sichuan University International Chinese Hard Tissue Society （ICHTS）.

Bone Research is supported by West China School of Stomatology, Sichuan University State Key Laboratory of Oral Diseases, Sichuan University International Chinese Hard Tissue Society （ICHTS） Type of manuscript accepted Review articles provide a comprehensive review of the state of the art on topics selected by the Editor-in-Chief. Reviews should not be submitted without contacting the Editorial Office. The word limit is 10 000 words （not including the title, author affiliations, abstracts, or references） and 200 references.

Titanium dioxide （TiO2） nanoparticles are produced for many different purposes, including development of therapeutic and diagnostic nanoparticles for cancer detection and treatment, drug delivery, induction of DNA double-strand breaks, and imaging of specific cells and subcellular structures. Currently, the use of optical microscopy, an imaging technique most accessible to biology and medical patholog36 to detect TiO2 nanoparticles in cells and tissues ex vivo is limited with low detection limits, while more sensitive imaging methods （transmission electron microscopy, X-ray fluorescence microscop~ etc.） have low throughput and technical and operational complications. Herein, we describe two in situ post- treatment labeling approaches to stain TiO2 nanoparticles taken up by the cells. The first approach utilizes fluorescent biotin and fluorescent streptavidin to label the nanoparticles before and after cellular uptake; the second approach is based on the copper-catalyzed azide-alkyne cycloaddition, the so-called Click chemistry, for labeling and detection of azide-conjugated TiO2 nanoparticles with alkyne- conjugated fluorescent dyes such as Alexa Fluor 488. To confirm that optical fluorescence signals of these nanoparticles match the distribution of the Ti element, we used synchrotron X-ray fluorescence microscopy （XFM） at the Advanced Photon Source at Argonne National Laboratory. Titanium-specific XFM showed excellent overlap with the location of optical fluorescence detected by confocal microscopy. Therefore, future experiments with TiO2 nanoparticles may safely rely on confocal microscopy after in situ nanoparticle labeling using approaches described here.

The National Laboratory of Solid State Mi- crostructures （NLSSM） was established in 1984, among the first group of state key laboratories established in China. The Laboratory is affili- ated with Nanjing University.

Ellis Reinherz and I were riding in a luxury Boston Coach to Long Island in New York State driven by a professional driver. This was not a vacation trip to one of the best leisure places in the United States. We were heading toward the National Laboratory at Brookhaven, where a powerful X-ray source is available in a facility called a synchrotron. The aim of our trip was to use a strong X-ray to shoot the crystals of the T cell receptor （TCR） we had just managed to grow after considerable effort for three-dimensional structure determination. In 1996, this was one of the hottest projects in structural biology as well as in immunology.