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1 result(s) for "活性氧代谢"
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外源ABA对短期H2O2胁迫下侧柏幼苗活性氧代谢及相关基因的影响
[目的] 研究外源ABA 处理对H2O2胁迫下侧柏幼苗活性氧代谢系统的影响,探讨ABA调控侧柏氧化胁迫的可能作用机制。[方法] 以侧柏幼苗为试验材料,采用水培方式,研究外施低浓度(0.5 μmol·L^-1)和高浓度(200 μmol·L^-1)ABA对100 mmol·L^-1 H2O2胁迫下侧柏幼苗活性氧代谢的影响。[结果] (1)100 mmol·L^-1 H2O2胁迫48 h 显著增加了侧柏幼苗叶片过氧化氢(H2O2)、丙二醛(MDA)、谷胱甘肽(GSH)和脯氨酸含量、抗氧化物酶(SOD和CAT)活性,而可溶性蛋白含量降低。(2)相较于高浓度200 μmol·L^-1 ABA,施加0.5 μmol·L^-1 ABA显著减少了H2O2胁迫下侧柏幼苗H2O2和MDA的积累,进一步提高了侧柏幼苗叶片SOD、POD和CAT活性,同时促进GSH、脯氨酸和可溶性蛋白的合成。(3)100 mmol·L^-1 H2O2胁迫处理48 h,侧柏幼苗叶片活性氧代谢相关基因Cu/Zn-SOD、CAT、GR、APX、MDAR和GST表达水平较对照CK均有显著性提高;正常和H2O2胁迫下侧柏幼苗外施0.5 μmol·L^-1 ABA相较于200 μmol·L^-1更有利于提高侧柏叶片活性氧代谢相关基因Cu/Zn-SOD、CAT、GR、APX、MDAR和GST的表达量。[结论] 低浓度0.5 μmol·L^-1 ABA有效地增强抗氧化系统的防御能力,减弱幼苗的氧化胁迫和膜脂过氧化水平,从而降低活性氧对侧柏的伤害。