Explore the vast range of titles available.

The image of Peter the Great casts a long shadow in modern Russian thought and culture. As important to modern Russia as the French Revolution is to France and the Reformation is to Germany, the image of this militaristic ruler, founder of St Petersburg, and czar of all Russia from 1689-1725 has been central to Russian history, literature, and art since the early 1700s.Riasanovsky, one of the foremost historians of Russia, traces the development of this image from 1700 to the present. Drawing examples from Russian historical accounts, literature, folklore, and the arts, he shows how the use of the image of Peter has reflected the changing cultural and political values of the Russian people.

Extracellular vesicles (EVs) are membrane-enclosed vesicles which play important role for cell communication and physiology. EVs are found in many human biological fluids, including blood, breast milk, urine, cerebrospinal fluid (CSF), ejaculate, saliva etc. These nano-sized vesicles contain proteins, mRNAs, microRNAs, non-coding RNAs and lipids that are derived from producing cells. EVs deliver complex sets of biological information to recipient cells thereby modulating their behaviors by their molecular cargo. In this way EVs are involved in the pathological development and progression of many human disorders, including neurodegenerative diseases. In this study EVs purified by ultracentrifugation from CSF of patients with Parkinson's disease (PD) and individuals of the comparison group were characterized using nanoparticle tracking analysis, flow cytometry and cryo-electron microscopy. Vesicular size and the presence of exosomal marker CD9 on the surface provided evidence that most of the EVs were exosome-like vesicles. Cryo-electron microscopy allowed us to visualize a large spectrum of extracellular vesicles of various size and morphology with lipid bilayers and vesicular internal structures. Thus, we described the diversity and new characteristics of the vesicles from CSF suggesting that subpopulations of EVs with different and specific functions may exist.

The invention and development of next or second generation sequencing methods has resulted in a dramatic transformation of ancient DNA research and allowed shotgun sequencing of entire genomes from fossil specimens. However, although there are exceptions, most fossil specimens contain only low (~ 1% or less) percentages of endogenous DNA. The only skeletal element for which a systematically higher endogenous DNA content compared to other skeletal elements has been shown is the petrous part of the temporal bone. In this study we investigate whether (a) different parts of the petrous bone of archaeological human specimens give different percentages of endogenous DNA yields, (b) there are significant differences in average DNA read lengths, damage patterns and total DNA concentration, and (c) it is possible to obtain endogenous ancient DNA from petrous bones from hot environments. We carried out intra-petrous comparisons for ten petrous bones from specimens from Holocene archaeological contexts across Eurasia dated between 10,000-1,800 calibrated years before present (cal. BP). We obtained shotgun DNA sequences from three distinct areas within the petrous: a spongy part of trabecular bone (part A), the dense part of cortical bone encircling the osseous inner ear, or otic capsule (part B), and the dense part within the otic capsule (part C). Our results confirm that dense bone parts of the petrous bone can provide high endogenous aDNA yields and indicate that endogenous DNA fractions for part C can exceed those obtained for part B by up to 65-fold and those from part A by up to 177-fold, while total endogenous DNA concentrations are up to 126-fold and 109-fold higher for these comparisons. Our results also show that while endogenous yields from part C were lower than 1% for samples from hot (both arid and humid) parts, the DNA damage patterns indicate that at least some of the reads originate from ancient DNA molecules, potentially enabling ancient DNA analyses of samples from hot regions that are otherwise not amenable to ancient DNA analyses.

The COVID-19 pandemic has tremendously affected higher education systems in Russia and all over the world, forcing to transform curriculum into an online format, which is a challenge for all the educational process participants. The current study discusses the implementation of online learning amid the COVID-19 pandemic in the Russian higher education context and investigates the challenges experienced by university teachers during this period to define their readiness for online education. To address the above-mentioned issues, a study was conducted in Peter the Great St. Petersburg Polytechnic University. A variety of methods of scientific and pedagogical research were used including systematic structural analysis, synthesis, work with research papers, the generalization of experience and experimental work, observation, surveys, etc., with 87 university teachers asked to respond to several sets of questions describing their online teaching experience after the launch of online education amid the COVID-19 pandemic. The analysis of the participants’ answers helped to identify the following main challenges experienced by university teachers: computer literacy level, the university electronic environment and support, academic staff readiness and students’ readiness for online learning, the last two being the most important hindering the implementation of the efficient online education process. It was also underlined by most respondents that methodological work of a teacher in a digital educational environment differs from conventional teaching methods. Thus, psychological, technological, methodological support and teachers’ professional development programs are of vital importance to minimize the negative impact of the rapid changes of the educational process and to ensure efficient online education.

All his attention was focused on the study of archaeological sites and the purchase of objects from burial grounds, and he also conducted the first archaeological excavations of burial mounds in Russia. Part of his scientific library was bought by the Academy of Sciences, part was brought to Moscow University, but most of the materials and collections of the scientist was lost during the fire in the building of the Kunstkamera Museum in 1747. [...]currently there are only 8 original drawings of the scientist in the Archive of the Russian Academy of Sciences in Saint Petersburg; 6 of which were made by Messerschmidt himself and 2 presumably made by draftsman K. G. Schulmann. Messerschmidt himself became the first artist who captured the Okunev culture sites of the Bronze Age \"The Statue of Ulug Khurtuyakh Tas\" and \"The Statue of KhysTas\" in the 20s of the 18th century.

This book highlights the main features and trends of Russian \"political\" thought in an era when sovereignty, state, and politics, as understood in Western Christendom, were non-existent in Russia, or were only beginning to be articulated. It concentrates on enigmatic authors and sources that shaped official perception of rulership, or marked certain changes of importance of this perception. Special emphasis is given to those written and visual sources that point towards depersonalization and secularization of rulership in Russia. A comparison with Western Christendom frames the argument throughout the book, both in terms of ideas and the practical aspects of state-building, allowing the reader to ponder Russia's differentia specifica .

This book highlights the main features and trends of Russian \"political\" thought in an era when sovereignty, state, and politics, as understood in Western Christendom, were non-existent in Russia, or were only beginning to be articulated. It concentrates on enigmatic authors and sources that shaped official perception of rulership, or marked certain changes of importance of this perception. Special emphasis is given to those written and visual sources that point towards depersonalization and secularization of rulership in Russia. A comparison with Western Christendom frames the argument throughout the book, both in terms of ideas and the practical aspects of state-building, allowing the reader to ponder Russia's differentia specifica .

Exosomes are a type of extracellular vesicles (EVs) secreted by multiple mammalian cell types and involved in intercellular communication. Numerous studies have explored the diagnostic and therapeutic potential of exosomes. The key challenge is the lack of efficient and standard techniques for isolation and downstream analysis of nanovesicles. Conventional isolation methods, such as ultracentrifugation, precipitation, filtration, chromatography, and immune-affinity-based approaches, rely on specific physical properties or on surface biomarkers. However, any of the existing methods has its limitations. Various parameters, such as efficacy, specificity, labor input, cost and scalability, and standardization options, must be considered for the correct choice of appropriate approach. The isolation of exosomes from biological fluids is especially challenged by the complex nature and variability of these liquids. Here, we present a comparison of five protocols for exosome isolation from human plasma: two chemical affinity precipitation methods (lectin-based purification and SubX ™ technology), immunoaffinity precipitation, and reference ultracentrifugation-based exosome isolation method in two modifications. An approach for the isolation of exosomes based on the phenomenon of binding and aggregation of these particles via clusters of outer membrane phosphate groups in the presence of SubX ™ molecules has been put forward in the present study. The isolated EVs were characterized based upon size, quantity, and protein content.

A remarkable snow climate exists on the Japanese islands of Honshu and Hokkaido near the Sea of Japan. Mean annual snowfall in this “gosetsu chitai” (heavy snow area) exceeds 600 cm (235 in.) in some near-sea-level cities and 1,300 cm (512 in.) in some mountain areas. Much of this snow falls from December to February during the East Asian winter monsoon when frequent cold-air outbreaks occur over the Sea of Japan. The resulting sea-effect precipitation systems share similarities with lake-effect precipitation systems of the Laurentian Great Lakes of North America, but are deeper, are modulated by the regional coastal geometry and topography, and can sometimes feature transversal mode snowbands. Snowfall can maximize in the lowlands or the adjoining mountains depending on the direction and strength of the boundary layer flow. Remarkable infrastructure exists in Japan for public safety, road and sidewalk maintenance, and avalanche mitigation, yet snow-related hazards claim more than 100 lives annually. For winter recreationists, there is no surer bet for deep powder than the mountains of Honshu and Hokkaido near the Sea of Japan in January, but the regional snow climate is vulnerable to global warming, especially in coastal areas. Historically, collaborative studies of sea- and lake-effect precipitation systems involving North American and Japanese scientists have been limited. Significant potential exists to advance our understanding and prediction of sea- and lake-effect precipitation based on studies from the Sea of Japan region and efforts involving meteorologists in North America, Japan, and other sea- and lake-effect regions.

Spiophanes bombyx (Claparède, 1870) from the Gulf of Naples, Tyrrhenian Sea, Italy, was the first described Spiophanes with fronto-lateral horns on the prostomium. It was also considered the only horned species occurring in European waters. Our sequence data of five gene fragments suggest the presence of two horned sibling Spiophanes species in northern Europe: S. cf. bombyx in the North and the Norwegian seas, and S. cf. convexus in Brittany, northern France, and Bay of Biscay, northern Spain. Spiophanes cf. bombyx worms are genetically close to a single examined specimen of S. bombyx from Venice Lagoon, Italy but their conspecificity should be verified by further study. Our sequence data show that horned Spiophanes from the North Pacific are genetically distant from horned European species, and that S. uschakowi Zachs, 1933, originally described from the Sea of Japan (East Sea) is a valid species. The data also suggest the presence of two horned sibling Spiophanes species in the North East Pacific: S. hakaiensis Radashevsky & Pankova, n. sp. distributed from Alaska south to about Point Conception, and S. norrisi Meißner & Blank, 2009, distributed from San Francisco Bay south to Baja California Sur, Mexico. Spiophanes from South America, morphologically similar to S. norrisi, are suggested to belong to a new species. Molecular data also suggest the presence of two sibling species among the worms from northern Europe identified by morphology as S. kroyeri Grube, 1860. Worms from the Barents Sea and northern part of the North Sea are tentatively referred to as S. cf. kroyeri; worms from the northern and central parts of the North Sea and from the Bay of Biscay, northern Spain, are tentatively referred to as S. cf. cirrata M. Sars in G.O. Sars, 1872. Sequence data also show that S. duplex from California is genetically different from morphologically similar worms from South America. The South American worms are referred to resurrected S. soederstroemi Hartman, 1953 which was originally described from off Rio Grande do Sul, Brazil, and then considered as a junior synonym of S. duplex. Analysis of divergence times of Spiophanes lineages suggested that the origin of the most recent common ancestor of horned Spiophanes with metameric nuchal organs was around 11.1 mya (95% HPD: 5.1-19.0 mya) and that the divergence of the North Atlantic and North Pacific lineages was around 7.9 mya (95% HPD: 4.1-13.3 mya). The North Atlantic lineage was estimated to have diverged 4.8 mya (95% HPD: 2.2-8.6 mya), resulting in the origin of S. cf. bombyx and S. cf. convexus. The North Pacific lineage was estimated to have diverged first by the isolation and speciation of S. norrisi 1.7 mya (95% HPD: 2.3-1.0 mya), and then by the isolation and speciation of S. uschakowi and S. hakaiensis n. sp. 1.3 mya (95% HPD: 2.0-0.7 mya). The estimates place the divergences soon after maximum glacial period in the North Pacific (2.4-3.0 mya).