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Sacred natural sites are culturally and environmentally significant areas to be researched in different cultural contexts. Hence, this research was conducted to investigate the indigenous mechanisms of preserving sacred natural sites in Guji Oromo, southern Ethiopia. Particularly, the study was conducted in Guji zone, Adoolaa Reeddee and Annaa Sorraa districts. Data were produced by interview (in-depth and key informants' interview), focus group discussion and transect walk. The data analysis was made qualitatively. The findings of the study demonstrate customary laws and oral declaration, taboos, customary punishment and social banishment are used as indigenous mechanisms used to preserve sacred natural sites in the study area.