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BackgroundQuaternary ammonium compounds (QACs), commonly used in cleaning, disinfecting, and personal care products, have recently gained worldwide attention due to the massive use of disinfectants during the COVID-19 pandemic. However, despite extensive use of these chemicals, no studies have focused on the analysis of QACs in human milk, a major route of exposure for infants.ObjectiveOur objectives were to identify and measure QACs in breast milk and evaluate early-life exposure to this group of compounds for nursing infants.MethodsEighteen QACs, including 6 benzylalkyldimethyl ammonium compounds (BACs, with alkyl chain lengths of C8-C18), 6 dialkyldimethyl ammonium compounds (DDACs, C8-C18), and 6 alkyltrimethyl ammonium compounds (ATMACs, C8-C18), were measured in breast milk samples collected from U.S. mothers. Daily lactational intake was estimated based on the determined concentrations for 0–12 month old nursing infants.ResultsThirteen of the 18 QACs were detected in breast milk and 7 of them were found in more than half of the samples. The total QAC concentrations (ΣQAC) ranged from 0.33 to 7.4 ng/mL (median 1.5 ng/mL). The most abundant QAC was C14-BAC with a median concentration of 0.45 ng/mL. The highest median ΣQAC estimated daily intake (EDI) was determined for <1-month old infants based on the average (using the median concentration) and high (using the 95th percentile concentration) exposure scenarios (230 and 750 ng/kg body weight/day, respectively).SignificanceOur findings provide the first evidence of the detection of several QACs in breast milk and identify breastfeeding as an exposure pathway to QACs for nursing infants.Impact statementOur findings provide the first evidence of QAC occurrence in breast milk and identify breastfeeding as one of the exposure pathways to QACs for nursing infants.

Soil extractable nitrate, ammonium, and organic nitrogen (N) are essential N sources supporting primary productivity and regulating species composition of terrestrial plants. However, it remains unclear how plants utilize these N sources and how surface-earth environments regulate plant N utilization. Here, we establish a framework to analyze observational data of natural N isotopes in plants and soils globally, we quantify fractional contributions of soil nitrate ( f NO3- ), ammonium ( f NH4+ ), and organic N ( f EON ) to plant-used N in soils. We find that mean annual temperature (MAT), not mean annual precipitation or atmospheric N deposition, regulates global variations of f NO3- , f NH4+ , and f EON . The f NO3- increases with MAT, reaching 46% at 28.5 °C. The f NH4+ also increases with MAT, achieving a maximum of 46% at 14.4 °C, showing a decline as temperatures further increase. Meanwhile, the f EON gradually decreases with MAT, stabilizing at about 20% when the MAT exceeds 15 °C. These results clarify global plant N-use patterns and reveal temperature rather than human N loading as a key regulator, which should be considered in evaluating influences of global changes on terrestrial ecosystems. Isotopic constraints reveal that soil nitrogen contribution to global plants is temperature-controlled, not by precipitation or nitrogen deposition. As temperatures rise, inorganic nitrogen becomes more important and preferred over organic nitrogen.

The impacts of enhanced nitrogen (N) deposition on the global forest carbon (C) sink and other ecosystem services may depend on whether N is deposited in reduced (mainly as ammonium) or oxidized forms (mainly as nitrate) and the subsequent fate of each. However, the fates of the two key reactive N forms and their contributions to forest C sinks are unclear. Here, we analyze results from 13 ecosystem-scale paired 15 N-labelling experiments in temperate, subtropical, and tropical forests. Results show that total ecosystem N retention is similar for ammonium and nitrate, but plants take up more labelled nitrate ( 20 15 25 %) ( mean minimum maximum ) than ammonium ( 12 8 16 %) while soils retain more ammonium ( 57 49 65 %) than nitrate ( 46 32 59 %). We estimate that the N deposition-induced C sink in forests in the 2010s  is 0.72 0.49 0.96  Pg C yr −1 , higher than previous estimates because of a larger role for oxidized N and greater rates of global N deposition. A study using paired 15 N tracers shows atmospheric N deposited in oxidized form is more likely retained by trees, while the reduced form is retained in soil. The authors argue that this is a greater contribution of deposited N to the global forest C sink than previously reported.

Data on bulk nitrogen deposition, plant foliar nitrogen and crop nitrogen uptake in China between ad  1980 and ad  2010 show that the average annual bulk deposition of nitrogen increased by approximately 8 kilograms of nitrogen per hectare during that period and that nitrogen deposition rates in the industrialized and agriculturally intensified regions of China are as high as the peak levels of deposition in northwestern Europe in the 1980s. Nitrogen on the up over China Atmospheric nitrogen emissions have increased substantially since the beginning of the industrial revolution, and the resulting deposition of nitrogen can have detrimental effects on human and ecosystem health. But little is known about the magnitude and environmental consequences of nitrogen deposition in today's fastest growing economy, China. This paper reports that average annual bulk deposition of nitrogen increased by 8 kg of nitrogen per hectare from the 1980s to the 2000s. Ammonium is the dominant form of nitrogen in bulk deposition, whereas the rate of increase is largest for nitrate deposition. Nitrogen deposition has also increased plant foliar nitrogen concentrations in semi-natural ecosystems and has elevated crop nitrogen uptake in long-term unfertilized croplands. China is experiencing intense air pollution caused in large part by anthropogenic emissions of reactive nitrogen 1 , 2 . These emissions result in the deposition of atmospheric nitrogen (N) in terrestrial and aquatic ecosystems, with implications for human and ecosystem health, greenhouse gas balances and biological diversity 1 , 3 , 4 , 5 . However, information on the magnitude and environmental impact of N deposition in China is limited. Here we use nationwide data sets on bulk N deposition, plant foliar N and crop N uptake (from long-term unfertilized soils) to evaluate N deposition dynamics and their effect on ecosystems across China between 1980 and 2010. We find that the average annual bulk deposition of N increased by approximately 8 kilograms of nitrogen per hectare ( P  < 0.001) between the 1980s (13.2 kilograms of nitrogen per hectare) and the 2000s (21.1 kilograms of nitrogen per hectare). Nitrogen deposition rates in the industrialized and agriculturally intensified regions of China are as high as the peak levels of deposition in northwestern Europe in the 1980s 6 , before the introduction of mitigation measures 7 , 8 . Nitrogen from ammonium (NH 4 + ) is the dominant form of N in bulk deposition, but the rate of increase is largest for deposition of N from nitrate (NO 3 − ), in agreement with decreased ratios of NH 3 to NO x emissions since 1980. We also find that the impact of N deposition on Chinese ecosystems includes significantly increased plant foliar N concentrations in natural and semi-natural (that is, non-agricultural) ecosystems and increased crop N uptake from long-term-unfertilized croplands. China and other economies are facing a continuing challenge to reduce emissions of reactive nitrogen, N deposition and their negative effects on human health and the environment.

Ice cores from low latitudes can provide a wealth of unique information about past climate in the tropics, but they are difficult to recover and few exist. Here, we report annually resolved ice core records from the Quelccaya ice cap (5670 meters above sea level) in Peru that extend back ~1800 years and provide a high-resolution record of climate variability there. Oxygen isotopic ratios (δ¹⁸O) are linked to sea surface temperatures in the tropical eastern Pacific, whereas concentrations of ammonium and nitrate document the dominant role played by the migration of the Intertropical Convergence Zone in the region of the tropical Andes. Quelccaya continues to retreat and thin. Radiocarbon dates on wetland plants exposed along its retreating margins indicate that it has not been smaller for at least six millennia.

Biologically available nitrogen limits photosynthesis in much of the world ocean. Organic matter (OM) stoichiometry had been thought to control the balance between the two major nitrogen removal pathways—denitrification and anammox—but the expected proportion of 30% anammox derived from mean oceanic OM is rarely observed in the environment. With incubations designed to directly test the effects of stoichiometry, however, we showed that the ratio of anammox to denitrification depends on the stoichiometry of OM supply, as predicted. Furthermore, observed rates of nitrogen loss increase with the magnitude of OM supply. The variable ratios between denitrification and anammox previously observed in the ocean are thus attributable to localized variations in OM quality and quantity and do not necessitate a revision to the global nitrogen cycle.

Partial-nitritation anammox (PNA) is a novel wastewater treatment procedure for energy-efficient ammonium removal. Here we use genome-resolved metagenomics to build a genome-based ecological model of the microbial community in a full-scale PNA reactor. Sludge from the bioreactor examined here is used to seed reactors in wastewater treatment plants around the world; however, the role of most of its microbial community in ammonium removal remains unknown. Our analysis yielded 23 near-complete draft genomes that together represent the majority of the microbial community. We assign these genomes to distinct anaerobic and aerobic microbial communities. In the aerobic community, nitrifying organisms and heterotrophs predominate. In the anaerobic community, widespread potential for partial denitrification suggests a nitrite loop increases treatment efficiency. Of our genomes, 19 have no previously cultivated or sequenced close relatives and six belong to bacterial phyla without any cultivated members, including the most complete Omnitrophica (formerly OP3) genome to date. ANaerobic AMMonium OXidation (ANAMMOX) combined with partial nitritation has been adopted for removal of ammonium from wastewater. Here, Speth et al . describe the bacterial metagenome of a partial-nitritation/anammox (PNA) reactor, and provide 23 draft genomes, 19 of which were previously uncharacterized/sequenced/cultivated.

The nitrogen isotopic composition of sedimentary rocks (δ 15 N) can trace redox-dependent biological pathways and early Earth oxygenation 1 , 2 . However, there is no substantial change in the sedimentary δ 15 N record across the Great Oxidation Event about 2.45 billion years ago (Ga) 3 , a prominent redox change. This argues for a temporal decoupling between the emergence of the first oxygen-based oxidative pathways of the nitrogen cycle and the accumulation of atmospheric oxygen after 2.45 Ga (ref.  3 ). The transition between both states shows strongly positive δ 15 N values (10–50‰) in rocks deposited between 2.8 Ga and 2.6 Ga, but their origin and spatial extent remain uncertain 4 , 5 . Here we report strongly positive δ 15 N values (>30‰) in the 2.68-Gyr-old shallow to deep marine sedimentary deposit of the Serra Sul Formation 6 , Amazonian Craton, Brazil. Our findings are best explained by regionally variable extents of ammonium oxidation to N 2 or N 2 O tied to a cryptic oxygen cycle, implying that oxygenic photosynthesis was operating at 2.7 Ga. Molecular oxygen production probably shifted the redox potential so that an intermediate N cycle based on ammonium oxidation developed before nitrate accumulation in surface waters. We propose to name this period, when strongly positive nitrogen isotopic compositions are superimposed on the usual range of Precambrian δ 15 N values, the Nitrogen Isotope Event. We suggest that it marks the earliest steps of the biogeochemical reorganizations that led to the Great Oxidation Event. Observations of strongly positive nitrogen isotopic compositions of sedimentary rocks in the 2.68-Gyr-old shallow to deep marine sedimentary deposit of the Serra Sul Formation suggest that oxygenic photosynthesis predated the Great Oxidation Event.

The health effects associated with particulate matter (PM) are extensively reported; nevertheless, limited research has explored the exact contributions of its chemical constituents to physical dysfunctional aging. This study assesses the relationships between prolonged exposure to significant ambient air pollutants, especially ammonium (NH 4 + ), and physical dysfunction in a nationally representative cohort of older Chinese adults. We investigated data from 14,641 participants aged 45 years or older in the 2015 wave of the China Health and Retirement Longitudinal Study (CHARLS), which was integrated with high-resolution air pollution data from the ChinaHighAirPollutants (CHAP) dataset. Physical dysfunction was evaluated by self-reported challenges in executing routine activities. After controlling for a wide range of confounders, associations among eight air contaminants (averaged from 2013 to 2015) and dysfunction risk were investigated using logistic regression models. Multicollinearity among covariates in the fully adjusted models was assessed using the generalized variance inflation factor (GVIF), with a threshold value of 5 adopted as the criterion to indicate potential collinearity. Sensitivity analyses—including exclusion of high-exposure participants, standardized z-score modeling, stratified subgroup evaluations, and multipollutant adjustments—were performed to assess the robustness of associations. Dose–response relationships were modeled using both quartile-based logistic regression and restricted cubic spline (RCS) models, revealing consistent and complementary trends. To estimate independent effects and address potential collinearity, we further constructed a multipollutant model adjusting for seven co-pollutants. In all and fully adjusted models, ambient ammonium (NH 4 + ) was the only air pollutant that demonstrated a significant and independent association with physical dysfunction (OR: 1.03; 95% CI: 1.01–1.05; p  < 0.05); no significant associations were found for the other pollutants. This association remained robust across multiple sensitivity analyses, including exclusion of extreme exposure (OR: 1.13; 95% CI: 1.08–1.17; p  < 0.001), z-score standardization (OR: 2.17; 95% CI: 1.57–2.98; p  < 0.001), and 5 stratified subgroup models. A significant dose–response relationship was identified both in quartile-based trend tests (p for trend < 0.001) and restricted cubic spline analysis (p for non-linearity < 0.001). Taken together, the monotonic trend from quartile analysis and the non-linear pattern from spline modeling suggest that even moderate exposure to NH4⁺ may contribute to physical dysfunction. Furthermore, multicollinearity diagnostics based on generalized variance inflation factors (GVIFs) indicated no evidence of problematic collinearity among covariates in the fully adjusted models (all GVIF < 5). Besides, the association remained significant and became stronger in a multipollutant model, highlighting the independent effect of NH 4 + beyond co-pollutant confounding (OR: 1.49; 95% CI: 1.26–1.76; p  < 0.001). Our findings indicate that NH 4 + , a significant secondary component of PM predominantly sourced from agricultural ammonia emissions, may uniquely contribute to the deterioration of physical function. It may be important to evaluate particle chemical makeup for analyzing health concerns, as there is no association for total PM mass. Long-lasting exposure to ambient NH 4 + has been independently associated with increased odds of physical dysfunction across older adults in China. These findings underscore the necessity for specific environmental strategies focused on ammonia reduction to alleviate age-related functional deterioration and foster healthy aging.

Slow release of nitrate by charred organic matter used as a soil amendment (i.e. biochar) was recently suggested as potential mechanism of nutrient delivery to plants which may explain some agronomic benefits of biochar. So far, isolated soil-aged and composted biochar particles were shown to release considerable amounts of nitrate only in extended (>1 h) extractions (\"slow release\"). In this study, we quantified nitrate and ammonium release by biochar-amended soil and compost during up to 167 h of repeated extractions in up to six consecutive steps to determine the effect of biochar on the overall mineral nitrogen retention. We used composts produced from mixed manures amended with three contrasting biochars prior to aerobic composting and a loamy soil that was amended with biochar three years prior to analysis and compared both to non-biochar amended controls. Composts were extracted with 2 M KCl at 22°C and 65°C, after sterilization, after treatment with H2O2, after removing biochar particles or without any modification. Soils were extracted with 2 M KCl at 22°C. Ammonium was continuously released during the extractions, independent of biochar amendment and is probably the result of abiotic ammonification. For the pure compost, nitrate extraction was complete after 1 h, while from biochar-amended composts, up to 30% of total nitrate extracted was only released during subsequent extraction steps. The loamy soil released 70% of its total nitrate amount in subsequent extractions, the biochar-amended soil 58%. However, biochar amendment doubled the amount of total extractable nitrate. Thus, biochar nitrate capture can be a relevant contribution to the overall nitrate retention in agroecosystems. Our results also indicate that the total nitrate amount in biochar amended soils and composts may frequently be underestimated. Furthermore, biochars could prevent nitrate loss from agroecosystems and may be developed into slow-release fertilizers to reduce global N fertilizer demands.