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The process of cable bolt anchoring the roof of a coal mine roadway involves the mixture of different types of resin cartridges. However, this procedure presently faces several shortcomings including insufficient cartridge breakage, non-ideal viscosity and annular thickness values of the anchoring body, short effective anchorage length, incomplete hole walls in the surrounding rock, the resin cartridges easily roll, bend, overlap, and pile up on the hole wall and do not properly react. This study presents a systematic analysis of the anchoring characteristics of roof cable bolts in a roadway with weak interlayers based on the research and development of synergistic components. The synergistic mechanism is identified. Numerical simulations are performed to demonstrate the dynamic flow field characteristics of the resin cartridge mixing, and laboratory tests are performed for comparison to determine a reasonable setting for the synergistic components. Field tests are conducted to comprehensively verify the working performance of the synergistic technology. The results show that the synergistic component can greatly improve the fluidity and uniformity of the resin cartridge reaction. The anchoring performance of the synergistic anchorage cable bolt used in the field is also significantly greater than that of ordinary anchoring cable bolts and higher than or equal to the engineering requirements. When an anchor hole is broken or collapses, the spacing of the synergistic components can be reasonably adjusted (i.e., by shortening the anchorage length) to allow the resin cartridge to evenly and densely fill the anchoring area, which greatly improves the anchoring system’s bearing capacity.HighlightsCable bolt resin anchoring on roadway roofs with weak interlayers is analyzed under typical on-field construction conditions.Anchoring synergistic components greatly improve the bearing capacity and energy absorption capacity of an anchoring system.On-field dynamic mixing guidance parameters are proposed for different types of resin cartridges in combination or used alone.Synergistic component spacing adjustments effectively improve the density and uniformity of an anchoring body, thus improving the anchoring quality.

Observing the structure and regeneration of the myelin sheath in peripheral nerves following injury and during repair would help in understanding the pathogenesis and treatment of neurological diseases caused by an abnormal myelin sheath. In the present study, transmission electron microscopy, immunofluorescence staining, and transcriptome analyses were used to investigate the structure and regeneration of the myelin sheath after end-to-end anastomosis, autologous nerve transplantation, and nerve tube transplantation in a rat model of sciatic nerve injury, with normal optic nerve, oculomotor nerve, sciatic nerve, and Schwann cells used as controls. The results suggested that the double-bilayer was the structural unit that constituted the myelin sheath. The major feature during regeneration was the compaction of themyelin sheath, wherein the distance between the 2 layers of cell membrane in the double-bilayer became shorter and the adjacent double-bilayers tightly closed together and formed the major dense line. The expression level of myelin basic protein was positively correlated with the formation of the major dense line, and the compacted myelin sheath could not be formed without the anchoring of the lipophilin particles to the myelin sheath.

This paper adds to the growing body of literature investigating Ukrainian women refugees’ framing of their experience as forced migrants. Using semi-structured interviews with 11 Ukrainian women refugees currently residing in Romania, we explore how these women navigate the complexities of adaptation and integration in Romania. We examine their perception of refugee status, negotiation of belonging to various transnational social spaces and problematization of their lived experiences as women, mothers and main decision-makers of their families. We are equally interested in identifying the factors that empower them to rebuild their lives and foster resilience in the host country. Our analysis shows that resilient adaptation is the result of an accumulation of everyday problem-solving, professional achievement and anchoring in re-invented roles as caregivers and advocates for Ukrainian communities.

A-kinase anchoring proteins (AKAPs) shape second-messenger signaling responses by constraining protein kinase A (PKA) at precise intracellular locations. A defining feature of AKAPs is a helical region that binds to regulatory subunits (RII) of PKA. Mining patient-derived databases has identified 42 nonsynonymous SNPs in the PKA-anchoring helices of five AKAPs. Solid-phase RII binding assays confirmed that 21 of these amino acid substitutions disrupt PKA anchoring. The most deleterious side-chain modifications are situated toward C-termini of AKAP helices. More extensive analysis was conducted on a valine-to-methionine variant in the PKA-anchoring helix of AKAP18. Molecular modeling indicates that additional density provided by methionine at position 282 in the AKAP18γ isoform deflects the pitch of the helical anchoring surface outward by 6.6°. Fluorescence polarization measurements show that this subtle topological change reduces RII-binding affinity 8.8-fold and impairs cAMP responsive potentiation of L-type Ca2+ currents in situ. Live-cell imaging of AKAP18γ V282M-GFP adducts led to the unexpected discovery that loss of PKA anchoring promotes nuclear accumulation of this polymorphic variant. Targeting proceeds via a mechanism whereby association with the PKA holoenzyme masks a polybasic nuclear localization signal on the anchoring protein. This led to the discovery of AKAP18ε: an exclusively nuclear isoform that lacks a PKA-anchoring helix. Enzyme-mediated proximity-proteomics reveal that compartment-selective variants of AKAP18 associate with distinct binding partners. Thus, naturally occurring PKA-anchoring-defective AKAP variants not only perturb dissemination of local second-messenger responses, but also may influence the intracellular distribution of certain AKAP18 isoforms.

Werner syndrome (WS) is a premature aging disorder caused by WRN protein deficiency. Here, we report on the generation of a human WS model in human embryonic stem cells (ESCs). Differentiation of WRN-null ESCs to mesenchymal stem cells (MSCs) recapitulates features of premature cellular aging, a global loss of H3K9me3, and changes in heterochromatin architecture. We show that WRN associates with heterochromatin proteins SUV39H1 and HP1α and nuclear lamina–heterochromatin anchoring protein LAP2β. Targeted knock-in of catalytically inactive SUV39H1 in wild-type MSCs recapitulates accelerated cellular senescence, resembling WRN-deficient MSCs. Moreover, decrease in WRN and heterochromatin marks are detected in MSCs from older individuals. Our observations uncover a role for WRN in maintaining heterochromatin stability and highlight heterochromatin disorganization as a potential determinant of human aging.

Genomic regions preferentially associate with regions of similar transcriptional activity, partitioning genomes into active and inactive compartments within the nucleus. Herewe explore mechanisms controlling genome compartment organization in Caenorhabditis elegans and investigate roles for compartments in regulating gene expression. Distal arms of C. elegans chromosomes, which are enriched for heterochromatic histone modifications H3K9me1/me2/me3, interact with each other both in cis and in trans, while interacting less frequently with central regions, leading to genome compartmentalization. Arms are anchored to the nuclear periphery via the nuclear envelope protein CEC-4, which binds to H3K9me. By performing genome-wide chromosome conformation capture experiments (Hi-C), we showed that eliminating H3K9me1/me2/me3 through mutations in the methyltransferase genes met-2 and set-25 significantly impaired formation of inactive Arm and active Center compartments. cec-4 mutations also impaired compartmentalization, but to a lesser extent. We found that H3K9me promotes compartmentalization through two distinct mechanisms: Perinuclear anchoring of chromosome arms via CEC-4 to promote their cis association, and an anchoring-independent mechanism that compacts individual chromosome arms. In both met-2 set-25 and cec-4 mutants, no dramatic changes in gene expression were found for genes that switched compartments or for genes that remained in their original compartment, suggesting that compartment strength does not dictate gene-expression levels. Furthermore, H3K9me, but not perinuclear anchoring, also contributes to formation of another prominent feature of chromosome organization, megabase-scale topologically associating domains on X established by the dosage compensation condensin complex. Our results demonstrate that H3K9me plays crucial roles in regulating genome organization at multiple levels.

A liquid crystal (LC) director distribution was numerically analyzed in 90-degree twisted nematic (TN) LC cells with a symmetric and an asymmetric azimuthal anchoring strength of the alignment substrate and the influence of anchoring strength on the electro-optical property of the TN cell was evaluated. The twist angle decreased with decreasing azimuthal anchoring strength and the LC orientation changed to a homogeneous orientation with the twist angle of 0 degrees in the LC cell with asymmetric azimuthal anchoring strength, specifically with the strong anchoring substrate and the weak anchoring substrate below a critical strength. The asymmetric anchoring LC cell was fabricated by using a poly (vinyl cinnamate) alignment substrate as the weak anchoring surface and a polyimide alignment substrate as the strong anchoring surface. The LC cell performed the dark–bright–dark switching of the transmittance in the crossed polarizers, since the homogeneous LC orientation changed to the TN orientation again with increasing the applied voltage. Therefore, it was experimentally confirmed that LC molecules rotated at 90 degrees in the plane on the alignment surface by the electric field perpendicular to the weak anchoring substrate.

Electrocatalysis by atomic catalysts is a major focus of chemical and energy conversion effort. Although transition-metal-based bulk electrocatalysts for electrochemical application on energy conversion processes have been reported frequently, anchoring the stable transition-metal atoms (e.g. nickel and iron) still remains a practical challenge. Here we report a strategy for fabrication of ACs comprising only isolated nickel/iron atoms anchored on graphdiyne. Our findings identify the very narrow size distributions of both nickel (1.23 Å) and iron (1.02 Å), typical sizes of single-atom nickel and iron. The precision of this method motivates us to develop a general approach in the field of single-atom transition-metal catalysis. Such atomic catalysts have high catalytic activity and stability for hydrogen evolution reactions. Single atom catalysts provide the most efficient metal atoms usage and afford active site homogeneity, but surface attachment has proven challenging. Here, the authors use triple-bond-rich graphdiyne to anchor nickel/iron atoms and show high hydrogen evolution electrocatalysis activities.

Redox cocatalysts play crucial roles in photosynthetic reactions, yet simultaneous loading of oxidative and reductive cocatalysts often leads to enhanced charge recombination that is detrimental to photosynthesis. This study introduces an approach to simultaneously load two redox cocatalysts, atomically dispersed cobalt for improving oxidation activity and anthraquinone for improving reduction selectivity, onto graphitic carbon nitride (C₃N₄) nanosheets for photocatalytic H₂O₂ production. Spatial separation of oxidative and reductive cocatalysts was achieved on a two-dimensional (2D) photocatalyst, by coordinating cobalt single atom above the void center of C₃N₄ and anchoring anthraquinone at the edges of C₃N₄ nanosheets. Such spatial separation, experimentally confirmed and computationally simulated, was found to be critical for enhancing surface charge separation and achieving efficient H₂O₂ production. This center/edge strategy for spatial separation of cocatalysts may be applied on other 2D photocatalysts that are increasingly studied in photosynthetic reactions.

Anchoring is á ubiquitous heuristic by which decision makers heavily rely on apiece of information (anchor) that appears prior to a decision. Yet, we know little about its role in strategic decisions. This study considers its influence on acquisition premiums by examining whether a focal premium decision may be anchored on the premium that another firm paid for the acquisition that directly preceded the focal acquisition in the same market because it presents a salient and compatible premium to decision makers. Our results support this premise, particularly when preceding acquisitions happened more recently and were similar in size to the focal deals, when focal deals were in a foreign market, and when acquirers lacked acquisition experience in the target market or had a higher acquisition rate.