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South American Camelids (SACs) make several material and non-material contributions to people and are a key component of the Andean biocultural heritage. From the perspective of the IPBES’ Conceptual Framework, SACs constitute the “nature” component in the complex system of interactions between human societies and the Andean mountain environment. There are four SAC living species today, two of which are wild, or Salqa, in the indigenous cosmovision: guanaco (Lama guanicoe) and vicuña (Vicugna vicugna). Llama (Lama glama) and alpaca (Vicugna pacos) were domesticated 5000 years ago, and are therefore Uywa, in the indigenous cosmovision. Both wild and domestic camelids were, and in several cases still are, the most highly appreciated resource for Andean livelihoods. Historically, camelids and their contributions have been used by Andean people since the peopling of the Americas over 11,000 years ago. In this paper, we present three case studies (chakus for vicuña management, llama caravans, and llama nanobodies) to bring attention to the essential role of vicuñas and llamas for Andean communities today, their intercultural linkages with the Western world, and telecoupling interactions.

Research carried out in the Carangas region of highland Bolivia has identified a surprising concentration of pre-Hispanic religious sites, which are linked both to ancient Andean cults of wak'a (sacred mountains, tutelary hills and mummified ancestors) and to the Incan settlement of the region. Of these sites, one ceremonial centre stands out for its unprecedented characteristics for the Andes.

Archaeological research carried out in the altiplano locale of Escaramayu (in the community of Escara, Potosí, Bolivia), revealed a prehispanic metallurgical establishment (ninth to fifteenth centuries AD) for the extractive processing of copper ores and, to a lesser extent, lead-silver ore exploited at the nearby Pulacayo mine. The number and variety of metallurgical equipment identified in this establishment for smelting metallic ore and for refining the metal indicate a deep level of technological experience and experimentation among the resident Escaramayu metalworkers during the Middle Horizon (MH) and Late Intermediate periods (LIP). In Escaramayu we find the first known antecedents of Andean wind furnaces (wayras) and a model of a prehispanic reverberatory furnace that was widely used in the southern Andean altiplano during the colonial period.

Quinoa ( Chenopodium quinoa ) agriculture has been a cornerstone of highland Andean diets for thousands of years, but it has received relatively little attention from archaeologists studying diet through stable isotope analysis. In this study, we present the largest sample published to date (n = 49) of archaeological carbon (δ 13 C) and nitrogen (δ 15 N) stable isotope ratios in quinoa, as well as single samples of cactus ( Opuntia spp.), wild bean ( Trifolium amabile ), and potato ( Solanum tuberosum ) from a Late Intermediate Period (cal ad 1250–1450) hillfort town in the western Lake Titicaca basin. Quinoa δ 15 N averages + 8.83‰±2.17, indicating that agricultural fields at this site were fertilized with camelid manure, but values were significantly higher in samples recovered from high-status compounds than low-status ones. This suggests that high-status groups within the community had larger camelid herds and/or older fields that had been improved with fertilizer for longer periods of time, possibly allowing their plants to be more productive than those of lower-status groups. Mean quinoa δ 13 C was − 23.95‰±0.72, which indicates that plants were not significantly more water-stressed than modern or historic comparative samples grown with similar methods. This concurs with paleoclimate data suggesting that the environment surrounding this settlement was in a period of drought recovery during the occupation. Alternatively, fertilizer may have allowed plants to combat the effects of drought without recycling sub-stomatal CO 2 . This study is an important contribution to research on foodways in the ancient Andes and non-cereal grain-dependent societies more broadly.

The phylum Bacteroidetes represents one of the most abundant bacterial groups of marine and freshwater bacterioplankton. We investigated the diversity of Bacteroidetes in water and sediment samples from three evaporitic basins located in the highlands of northern Chile. We used both 16S rRNA gene clone libraries created with targeted Bacteroidetes‐specific primers and separation of specifically amplified gene fragments by denaturing gradient gel electrophoresis (DGGE). DGGE analysis revealed a reduced richness of these organisms in samples from Salar de Huasco (two to four DGGE bands) increasing in Salar de Ascotán (two to seven DGGE bands) and Laguna Tebenquiche at Salar de Atacama (four to eight DGGE bands). Cluster analysis (WPGMA) of DGGE bands showed that bands from Salar de Huasco and Salar de Ascotán grouped together and samples from Salar de Atacama formed separate clusters in water and sediment samples, reflecting different Bacteroidetes communities between sites. Most of the sequences analyzed belonged to the family Flavobacteriaceae and clustered with the genera Psychroflexus, Gillisia, Maribacter, Muricauda, Flavobacterium, and Salegentibacter. The most abundant phylotype was highly related to Psychroflexus spp. and was recovered from all three study sites. The similarity of the analyzed sequences with their closest relatives in GenBank was typically <97% and notably lower when compared with type strains, demonstrating the unique character of these sequences. Culture efforts will be necessary to get a better description of the diversity of this group in saline evaporitic basins of northern Chile.

Background The Andean Altiplano hosts a repertoire of high-altitude lakes with harsh conditions for life. These lakes are undergoing a process of desiccation caused by the current climate, leaving terraces exposed to extreme atmospheric conditions and serving as analogs to Martian paleolake basins. Microbiomes in Altiplano lake terraces have been poorly studied, enclosing uncultured lineages and a great opportunity to understand environmental adaptation and the limits of life on Earth. Here we examine the microbial diversity and function in ancient sediments (10.3–11 kyr BP (before present)) from a terrace profile of Laguna Lejía, a sulfur- and metal/metalloid-rich saline lake in the Chilean Altiplano. We also evaluate the physical and chemical changes of the lake over time by studying the mineralogy and geochemistry of the terrace profile. Results The mineralogy and geochemistry of the terrace profile revealed large water level fluctuations in the lake, scarcity of organic carbon, and high concentration of SO 4 2- -S, Na, Cl and Mg. Lipid biomarker analysis indicated the presence of aquatic/terrestrial plant remnants preserved in the ancient sediments, and genome-resolved metagenomics unveiled a diverse prokaryotic community with still active microorganisms based on in silico growth predictions. We reconstructed 591 bacterial and archaeal metagenome-assembled genomes (MAGs), of which 98.8% belonged to previously unreported species. The most abundant and widespread metabolisms among MAGs were the reduction and oxidation of S, N, As, and halogenated compounds, as well as aerobic CO oxidation, possibly as a key metabolic trait in the organic carbon-depleted sediments. The broad redox and CO 2 fixation pathways among phylogenetically distant bacteria and archaea extended the knowledge of metabolic capacities to previously unknown taxa. For instance, we identified genomic potential for dissimilatory sulfate reduction in Bacteroidota and α- and γ-Proteobacteria, predicted an enzyme for ammonia oxidation in a novel Actinobacteriota, and predicted enzymes of the Calvin–Benson–Bassham cycle in Planctomycetota, Gemmatimonadota, and Nanoarchaeota. Conclusions The high number of novel bacterial and archaeal MAGs in the Laguna Lejía indicates the wide prokaryotic diversity discovered. In addition, the detection of genes in unexpected taxonomic groups has significant implications for the expansion of microorganisms involved in the biogeochemical cycles of carbon, nitrogen, and sulfur. EPcqnK6xUD66cr6MUL2q3U Video Abstract

Clarifying the taxonomic status and distribution of endangered species is crucial to their conservation. In this study, we contrasted different lines of evidence (morphology, mtDNA, and nucDNA: microsatellites and SNP) to clarify the taxonomic status of endangered Telmatobius water frog species and unidentified populations that inhabit the Salt Puna in Chile. We studied population differentiation and species divergence by performing morphometric, population genetic and species delimitation analyses. The results confirmed the species status of Telmatobius fronteriensis and T. philippii, as they exhibited morphometric, mitochondrial and genomic SNP divergence. Although Bayes factor delimitation analysis indicated that the Telmatobius populations of Ascotán and Carcote could represent a new species, their few mitochondrial differences and similar morphology with respect to T. philippii suggested otherwise. Instead, they can be considered an evolutionarily significant unit of T. philippii that has differentiated from the type locality. These results extend the geographic distribution of T. philippii, which is categorized as critically endangered by the IUCN.

In the western Andes, climate changes have led to drastic ecological changes during the Pleistocene and Holocene. Given the debate surrounding precipitation pattern changes and the lack of research on lakes in the Chilean Altiplano, this study aims to assess recent climate changes. The paper presents an innovative methodology based on Google Earth Engine (GEE), utilizing fluctuations in water levels in endorheic lakes as natural precipitation indicators. Three lakes (Chungará, Miscanti, and Miniques) in isolated drainage systems were studied, where changes in water levels directly reflect rainfall variations. Data from Landsat-OLI 8, Landsat-ETM+, Landsat-TM 5, and MODIS spanning 31 years were processed using the Google Earth Engine platform. The shapes of the water bodies were extracted using hue saturation value (HSV) composites. The surface areas of the lakes were compared with precipitation data from national meteorological stations and the Tropical Rainfall Measuring Mission (TRMM) using linear regression analyses. Both lake area and rainfall volume showed a decrease over time, with varying trends depending on environmental conditions. However, the analysis consistently indicates a reduction in the area and volume of Chilean lakes corresponding to observed rainfall patterns over the past three decades.

This study compares temperature, precipitation, and other climate variables from six widely used climate reanalysis products to inform ice‐core climate proxy record calibration in the Altiplano region of the central Andes. The reanalyzes are the European Reanalysis version 5 (ERA5), European Reanalysis Interim, Modern‐Era Retrospective analysis for Research and Applications (MERRA2), Japanese 55‐year Reanalysis, Climate Forecast System Reanalysis and version 2 extension, and NCEP/NCAR Reanalysis version 1. These data products are validated against observations from automatic weather stations on the Quelccaya Ice Cap, Peru (5,650 m a.s.l) and Chacaltaya, Bolivia (5,238 m a.s.l), in addition to lower sites ranging in elevation 2,500–4,900 m a.s.l. Our results suggest that ERA5 provides the most robust overall depiction of temperature and precipitation across the study domain, and the data set is particularly useful for its back‐extension to 1950. However, MERRA2 produces lower precipitation error scores owing to a gaged‐based bias correction. An examination of ERA5 vertical atmospheric profiles for a latitudinal transect over Quelccaya shows considerable variability, including across major El Niño events, suggesting the need for caution when interpreting isotopic signatures in ice cores. Key Points European Reanalysis version 5 (ERA5) affords a robust overall climate depiction for a domain spanning the central Andes in comparison to other reanalyzes Modern‐Era Retrospective analysis for Research and Applications version 2 produces lower error scores than ERA5 against precipitation observations, but improvement is due to a gauge‐based bias correction ERA5 vertical profiles over Quelccaya for major El Niños suggest need for caution interpreting El Niño Southern Oscillation signatures in ice cores

Frustulia tunariensis sp. nov. is originated from a high-altitude peatland in the Tunari Cordillera, a branch of the Andean range in Bolivia. The new taxon is distinguished by the thick longitudinal ribs, the globose polar nodule with faint helictoglossa that does not produce an apical extension, and by the high areola and stria density, not found in any of the morphologically closely related taxa. Features of the folded valvocopula, such as the presence of a siliceous membrane as pars interior, and poroids present in the tube-like portion opening as slits to the valve interior and as a single row of poroids to the exterior, are also unique characters in the new taxon. Based on a literature review, a comparison of the newly proposed species with morphologically similar taxa was made. Also, published information shows the potential of girdle bands to distinguish groups of species and species themselves within Frustulia. Likewise, remarks on the ecological and distribution aspects of Frustulia in the Bolivian Altiplano are included, focusing on taxonomic quality, geographic coverage and sampling, and potential to represent the genus in the high Bolivian Andean plateau.