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This study is aimed to cost-effectively produce bacterial cellulose (BC) and impart it with antimicrobial activity by introducing bactericidal plant extracts into its three-dimensional matrix through an ex situ modification strategy. Briefly, the fruit wastes were utilized as the carbon source for BC production by Gluconacetobacter hansenii, followed by impregnation of bioactive extracts of Anogeissus dhofarica and Withania somnifera plants. FE-SEM and FTIR analyses showed the successful synthesis of BC-Anogeissus dhofarica (BC-A) and BC-Withania somnifera (BC-W) composites. Both BC-A and BC-W composites held 87% and 75% water of their dry weight, respectively, and each composite retained about 50% of originally absorbed water after 24 h. The bactericidal activities of BC-A and BC-W composites were investigated against Staphylococcus aureus and Escherichia coli as model Gram-positive and Gram-negative pathogens, respectively. Both BC-A and BC-W composites showed impressive antibacterial activity against S. aureus by producing a clear inhibition zone of 13 mm and 6 mm, respectively. Although no clear inhibition zone was observed against E. coli, both composites reduced its growth up to 50% of its live cell density. This is the first report of exploring the antibacterial activities of A. dhofarica and W. somnifera plant extracts and developing their composites with BC, which could be of vital importance for natural products and biopolymer researchers to develop biomedical materials.Graphical abstractToC. Low-cost production and ex situ impregnation of bioactive plant-extract into bacterial cellulose matrix showing antibacterial activity.

Two novel proanthocyanidins, (2R, 3R)-(+)-Gallocatechin-(4β → 8)4-(2R, 3R)-(+)-gallocatechin (compound 1) and 3-O-galloyl-(2S, 3S)-(–)-epicatechin-(4α → 8)-[3-O-galloyl-(2S, 3S)-(–)-epicatechin (4α → 8)]2-(2S, 3S)-(–)-epicatechin (compound 2), were structurally characterized from leaves of Anogeissus pendula . The structures were determined by ultraviolet spectroscopy (UV), proton nuclear magnetic resonance ( 1 H NMR), 13 C NMR, and heteronuclear multiple bond correlation. Molinspiration and Osiris property explorer applications were used to predict bioactivity and drug score. Drug scores of 0.08 and 0.05 were predicted for compounds 1 and 2, respectively. Predicted bioactivity scores were high. Due to their molecular weight, chemical structure, and conformation, the newly discovered proanthocyanidins possess an inclination to interact with proteins. Based on this premise, both compounds were subjected to in vitro testing against ruminal enzymes. They exhibited significant inhibition activities ( p < 0.01) with a range of half maximal effective concentration (EC 50 ) of 14.80–17.88 mg/mL of glutamic oxaloacetic transaminase in both protozoa and bacteria fractions. The ruminal glutamic pyruvic transaminase activity was significantly inhibited ( p < 0.01) from EC 50 12.59–16.29 mg/mL, and R-cellulase inhibition was recorded with EC 50 18.20–21.98 mg/mL by compounds 1 and 2, respectively. Protease activity decreased with increasing incubation time and concentration of both compounds. The novel proanthocyanidins have potential roles in improving feed conversion ratios and in drug development.

In the current study, methanol (ADAM) extracts and their fractions, including chloroform (ADAC), ethyl acetate (ADAE), n-hexane (ADAH), and aqueous (ADAA) fractions, were prepared from aerial parts of Anogeissus dhofarica and evaluated for phytochemical assessment, high-resolution electrospray ionization mass spectrometry (HR-ESI-MS) analysis, and in vitro bioassays. The qualitative analysis determined that, except alkaloids, all the representative groups were found to be present in the analyzed samples. Samples under quantitative study displayed the highest amount of total phenolic contents in the ADAE fraction, while total flavonoid contents were highest in the ADAM extract. The ADAM extract was subjected to HR-ESI-MS to identify the chemical constituents that presented twenty-two bioactive ingredients, outlined for the first time from A. dhofarica, mainly contributed by sub-class flavanones. In the case of antimicrobial activity, the ADAE extract revealed an effective zone of inhibition (ZOI) against the Gram-positive bacterial strain (Staphylococcus aureus) with an MIC value of 0.78 ± 0.3 mg/mL, while the ADAA extract exhibited higher ZOI (34 ± 0.12 mm) against the fungal strain Candida kruzei with an MIC of 0.78 mg/mL. In the DPPH (2,2-diphenyl-1-picrylhydrazyl) analysis, the ADAE extract exhibited a maximum scavenging potential with an IC50 of 9.8 ± 1.2 μg/mL, succeeded by the ADAM extract with an IC50 of 17.4 ± 0.4 μg/mL free radical scavenging capability. In the antidiabetic assessment, the ADAE extract was the most effective, with an IC50 of 6.40 ± 0.1 μg/mL, while the same extract demonstrated prominent activity with 30.8% viability and an IC50 of 6.2 ± 0.3 μg/mL against breast cancer cell lines. The brine shrimp lethality assay demonstrated a correlation with the in vitro cytotoxicity assay, showing the ADAE extract as the most active, with a 70% mortality rate and an LC50 of 300.1 μg/mL. In conclusion, all the tested samples, especially the ADAE and ADAM extracts, have significant capabilities for the investigated activities that could be due to the presence of the bioactive compounds.

It is important to estimate soil organic carbon (SOC) content of natural forests for an understanding of the Himalayan ecosystem. In this study SOC concentration was evaluated at three different soil depths (0–10, 10–20 and 20–30 cm) under Anogeissus latifolia (site I) and Pinus roxburghii (site II) forest stands in Himachal Pradesh, India. SOC (%) in these forests ranged from 0.37% to 2.20% up to 30 cm soil depth and was higher at site I compared to site II. Tree density was also more at site I than site II. The present study shows that the tree species can influence SOC of the forest ecosystem, but other environmental parameters such as soil type, moisture and pH are also responsible for changes in the soil carbon sequestration potential. Carbon sequestration in the study area showed significant contribution in minimizing the increase in carbon dioxide in the atmosphere and improving soil quality.

Several polyphenol-rich Terminalia species (Combretaceae) are known to accelerate wound healing. Recently, the Omani medicinal plant Anogeissus dhofarica (now Terminalia dhofarica) was attributed to the genus Terminalia based on phylogenetic studies. Leaves, bark, and extracts of T. dhofarica are traditionally used for various medicinal purposes, including wound treatment and personal hygiene. In the present study, the phytochemical profile of leaves from T. dhofarica was evaluated by ultra-high-performance liquid chromatography coupled with electrospray ionization high-resolution mass spectrometry (UHPLC-ESI-HRMS) and nuclear magnetic resonance (NMR) spectroscopy. Simple phenolics, polyphenolics (e.g., flavonoids and tannins) and their glucosides were characterized as major metabolite classes. In addition, 20 phenolics were isolated and structurally identified. Nine of these compounds were never described before for T. dhofarica. For the first time, we provide complete NMR data for 1-O-galloyl-6-O-p-coumaroyl-d-glucose (1). Biological screening demonstrated moderate efficacy against the Gram-negative bacterium Aliivibrio fischeri, the phytopathogenic fungus Septoria tritici, and the oomycete Phytophthora infestans. In summary, the data expand the knowledge of the phytochemistry of the underexplored species T. dhofarica and underscore its potential for therapeutic applications, particularly in the context of traditional medicine.

This study investigated the principal leaf protein (rubisco) solubilization and in vitro ruminal enzyme activity in relation to the molecular structure of proanthocyanidins extracted from leaves of Anogeissus pendula and Eugenia jambolana. Six proanthocyanidin fractions were extracted by 50% (v/v) methanol–water followed by 70% (v/v) acetone–water and then distilled water from leaves of A. pendula (AP) and E. jambolana (EJ) to yield EJ–70, EJ–50, EJ–DW, AP–70, AP–50 and AP–DW. Fractions were examined for their molecular structure and their effects on sheep ruminal enzymes and solubilization of rubisco in vitro. All fractions significantly (p < 0.05) inhibited the activity of ruminal glutamic oxaloacetic transaminase and glutamic pyruvic transaminase. The fractions AP–50 and EJ–50 significantly inhibited the activity of the R-cellulase enzyme. Most of the fractions inhibited R-glutamate dehydrogenase activity (p < 0.05) by increasing its concentration, while protease activity decreased by up to 58% with increasing incubation time and concentration. The solubilization of rubisco was observed to be comparatively higher in A. pendula (16.60 ± 1.97%) and E. jambolana (15.03 ± 1.06%) than that of wheat straw (8.95 ± 0.95%) and berseem hay (3.04 ± 0.08%). A significant (p < 0.05) increase in protein solubilization was observed when wheat straw and berseem hay were supplemented with A. pendula and E. jambolana leaves at different proportions. The efficiency of microbial protein was significantly (p < 0.05) greater with the supplementation of leaves of A. pendula in comparison to E. jambolana. The overall conclusion is that the proanthocyanidins obtained from E. jambolana exhibited greater inhibitory activities on rumen enzymes, whereas A. pendula recorded higher protein solubilization. Thus, PAs from A. pendula and E. jambolana appear to have the potential to manipulate rumen enzyme activities for efficient utilization of protein and fiber in ruminants.

The present study investigates the effect of an aqueous extract of (AEAL) on normotensive Wistar rats and its chronic antihypertensive effects in L-NAME-induced hypertensive rats by using a non-invasive tail-cuff model. The effects of AEAL (50mg/kg) and NaCl 0.9% on blood pressure were investigated by daily oral administration in normotensive Wistar rats over four weeks. L-NAME-induced hypertensive rats were produced by L-NAME (40mg/kg) daily oral administration for two weeks. For chronic antihypertensive effects, induced hypertensive rats have received L-NAME in combination with AEAL (10 or 50mg/kg/day) for two following weeks. In normotensive rats, daily administration of AEAL (50mg/kg) has no significant effect on their blood pressure, which was similar to that of the control group. L-NAME's daily oral administration induces a progressive increase in systolic blood pressure (SBP) from 115.8 ± 7.9mmHg to 153.5 ± 4.6mmHg after two weeks, which was maintained to the end of the treatment. In L-NAME-induced hypertensive rats, AEAL (50mg/kg/day) significantly decreases the SPB from 160.0 ± 5.8 mmHg to 108.8 ± 2.7mmHg after only four days of administration. However, the lower dose of AEAL (10mg/kg) also normalized the SBP of L-NAME-induced hypertensive rats but only evident after seven days of administration. Moreover, AEAL does not effect on the serum biochemical parameters (ALAT, ASAT, CREAT, etc.) and any macroscopic adverse effect was detected on the sensible organs involved during hypertension. In the aorta rings from treated rats, AEAL (50mg/kg/day) alone or in combination with L-NAME has enhanced the vasodilation effect of acetylcholine. However, the vasodilation effect of AEAL alone or in association with L-NAME has enhanced the sodium nitroprusside effect in treated rat aorta rings after autopsy. These findings suggest that AEAL affords significant antihypertensive effects against L-NAME-induced hypertensive rats without modification of serum parameters and deleterious effects.

This study was designed to explore the micro-anatomical characteristics of leaf and petiole of two unstudied West African native monotypic genera - Anogeissus and Qusqualis in Combretaceae with a view to characterizing the taxa, providing useful research-based information for identification. The samples were prepared following standard procedures. The light microscopic study of the transverse sections of the leaves in A. leiocarpus revealed a thick upper and lower cuticle while the cuticle on both leaf surfaces in Q. indica remain thin. The midrib vascular bundle of the leaf in Q. indica is characterized by arc-shape and starch grains were observed in the parenchyma cells of the ground tissue. The uniseriate rows of lamina epidermis were oval, squared, rectangular or polygonal in Q. indica while it was rectangular or slightly oval in A. leiocarpus. Vascular bundle in the leaves and petioles of both taxa were collateral. Simple, slender and short unicellular non-glandular trichomes were also observed in the leaf and petiole micro-anatomy of both taxa. It was concluded that the micro-anatomical features of the leaves and petioles are important parameters of characterization, used in the identification of the studied taxa.

Schleichera oleosa (Lour.) Oken is an important tree species and holds great ecological and economical importance in the lower Western Himalaya of India. Unfortunately, its populations in the region are facing decline due to range of natural (extreme weather/edaphic conditions and invasion of invasive alien species) and human-induced disturbances (grazing, lopping for fodder and fuel wood consumption). To address this issue, a study was conducted in the Doon Valley of the lower Western Himalaya to evaluate the population status and regeneration pattern of this species. Additionally, the study aimed to identify and map the potential habitat of the Indian lac tree in this region. Therefore, a detailed vegetation study was conducted using the stratified random sampling procedure at elevations between 250 and 1000 m. The relative values of frequency, density, and abundance were used to determine the importance value index (IVI) for tree species. These IVI data was further used to re-congregate the quadrates to know the forest association; following this the multivariate analysis was conducted using PAST 4.0 software to group vegetation into different catagories. In the present study, we categorized the tree species into 8 distinct plant associations as Anogeissus − Lannea − Adina, Lannea − Mallotous − Schleichera, Schleichera − Lannea − Syzygium , Schleichera − Anogeissus − Adina , Holoptelea − Lagerstroemia − Schleichera, Schleichera − Cordia − Albizia , Shorea − Mallotous − Cassia , and Azadirachta − Senegalia − Tectona association on the basis of species dominance. The study observed a total of 177 species in S. oleosa forests, including both native and exotic species. Among these, 89 species (50.28%) were classified as trees, 31 species (17.51%) as shrubs, 37 species (20.90%) as herbs, and 20 species (11.30%) as climbers. In various associations, the overall density of S. oleosa trees ranged from 16 to 86 individuals per hectare, while the total tree density ranged from 213 to 540 individuals per hectare. The maximum density of saplings for S. oleosa was recorded as 253 individuals per hectare, while for seedlings, it was 2750 individuals per hectare. Of the eight association types that have existed in the area, the  Azadirachta − Senegalia − Tectona association showed a complete failure of regeneration (absence of seedlings or saplings), while the  Holoptelea − Lagerstroemia − Schleichera , Schleichera − Cordia − Albizia and Shorea − Mallotous − Cassia association showed absence of saplings. In order to create a species conservation and management plan, we utilized the MaxEnt model to develop a potential distribution map for the species. The results obtained from this model were quite satisfactory, with an AUC mean of 0.929. The model determined that an area of 152.85 km 2 is highly suitable for the species and could potentially be utilized for species reintroduction. Moreover, the present study emphasizes the importance of regularly monitoring species populations to effectively plan the conservation and restoration efforts. It also highlights the need to develop standardized propagation techniques for mass multiplication, conserve species both in their natural habitats and in controlled environments, and raise awareness about these species among the local community.

BACKGROUND AND AIMS: In the dry tropics, vegetative phenology varies widely with tree characteristics and soil conditions. The present work aims to document the phenological diversity of flowering and fruiting with reference to leafing events in Indian dry-tropical tree species. METHODS: Nine tree species, including one leaf-exchanging and eight deciduous showing varying leafless periods, were studied. Monthly counts of leaves, flowers and fruits were made on 160 tagged twigs on ten individuals of each species for initiation, completion and duration of different phenological events through two annual cycles. KEY RESULTS: Variation in flowering relative to leaf flushing (which occurred just prior to or during a hot, dry summer) revealed five flowering types: summer flowering (on foliated shoots), rainy-season flowering (on foliated shoots following significant rains), autumn flowering (on shoots with mature leaves), winter flowering (on shoots undergoing leaf fall) and dry-season flowering (on leafless shoots). Duration of the fruiting phenophase was shortest (3-4 months) in dry-season and winter-flowering species, 6-9 months in rainy-and autumn-flowering species, and maximum (11 months) in summer-flowering species. A wide range of time lag (<1 to >8 months) between the start of vegetative (first-leaf flush) and reproductive (first-visible flower) phases was recorded in deciduous species; this time lag was correlated with the extent of the leafless period. A synthesis of available phenological information on 119 Indian tropical trees showed that summer-flowering species were most abundant (56 % of total species) amongst the five types recognized. CONCLUSIONS: The wide diversity of seasonal flowering and fruiting with linkages to leaf flush time and leafless period reflect the fact that variable reproductive and survival strategies evolved in tree species under a monsoonic bioclimate. Flowering periodicity has evolved as an adaptation to an annual leafless period and the time required for the fruit to develop. The direct relationship between leafless period (inverse of growing period) and time lag between onset of vegetative and reproductive phases reflects the partitioning of resource use for supporting these phases. Predominance of summer flowering coupled with summer leaf flushing seems to be a unique adaptation in trees to survive under a strongly seasonal tropical climate.