Explore the vast range of titles available.

Artocarpus altilis, A. Champeden, and Artocarpus heterophylus are popular species in Indonesia, and are commonly used as traditional medicine. This study aims to evaluate the diuretic effects of the ethyl acetate fraction of these three species on normotensive Wistar rats. The ethyl acetate fraction was prepared by a liquid–liquid extraction method. To evaluate diuretic effects, the sixty rats were divided into normal (distilled water), negative (4.5% NaCl), positive control (furosemide 5 mg/kg), and the testing groups. The testing groups were orally given the ethyl acetate fraction of A. altilis, Artocarpus champeden, and A. heterophylus at three dose levels of 25, 50, and 100 mg/kg. All animals were orally given 4.5% NaCl at a dose of 2 mL/200 g except the normal group; then the animals were given drugs according to group doses. Urine volume and electrolyte levels produced by the testing groups were compared to those of the control group. The concentration and ratio of ions were calculated to determine the natriuretic and carbonic anhydrase activities. Results: The ethyl acetate fractions of the three Artocarpus species at 100 mg/kg dose were more active than the standard furosemide (p < 0.05) to increase urinary excretion. Furthermore, at doses of 50 and 100 mg/kg, the fraction significantly increased the excretion of Na+, K+, and Cl- ions more than the standard (p < 0.05). The testing groups showed good natriuretic and carbonic anhydrase activities. The ethyl acetate fraction of A. altilis, A. champeden, and A. heterophylus leaves performed good diuretic and natriuretic activities. Therefore, the fractions can be considered as potential natural diuretic medicines.

Artocarpus is a plant genus widely distributed from Southeast Asia to Oceania. Notably, 34 of 45 Artocarpus species are in Indonesia, especially on Kalimantan Island. Therefore, Kalimantan Island is reconstructed as the center of the diversity of Artocarpus . Some Artocarpus species, including jackfruit ( A. heterophyllus Lam.) and cempedak ( A. integer [Thunb.] Merr.), are widely cultivated in the community. These plants have monoecious flowers, which makes cross-pollination between species possible. Nangkadak, the progeny of a cross between mini-jackfruit and local cempedak, was developed at the Mekarsari Fruit Garden. This study analyzed the genetic diversity of the progeny of A. heterophyllus  ×  A. integer crosses using vegetative characters and microsatellite markers. Microsatellite markers were used to analyze the phylogeny of six accessions of nangkadak and their parents. DNA was extracted from mature leaves, and PCR amplification was performed using Artocarpus microsatellite primers. Eight primers—MAA178, MAA54a, MAA140, MAA156, MAA145, MAA182, MAA54b, and MAA122—amplified 47 alleles, with an average of 5.8 alleles per locus. The amplification results showed that the microsatellite markers were polymorphic, which indicates kinship between accessions, as observed in the dendrogram of alleles identified from the electropherogram. The analysis of microsatellite markers also suggests that the cross between mini-jackfruit and local cempedak produces an allotetraploid progeny, as indicated by the maximum number of successful alleles amplified at the MAA178 and MAA156 loci. The phylogenetic analysis showed that nangkadak is more closely related to mini-jackfruit, used as the female parent than cempedak.

Background: Mandai, the fermented inner skin of cempedak (Artocarpus integer), may have further use as industrial ingredient while maintaining its antioxidative capacity. To promote fermentation, Lactobacillus casei was induced as the starter culture. This research was carried out (i) to investigate the effect of temperature on yield, chemical properties, and antioxidant activity of starter induced fermented mandai powder, (ii) to find the best drying temperature for the powder, and (iii) to find correlations between phenolic contents and antioxidant activity of the powder. Methods: The drying temperature was used as the variable, and was set at 45, 50, and 55°C at a fixed duration of 18 hours. The control was spontaneously fermented mandai dried at 50°C for 18 hours. Total phenolic content (TPC), hydrolyzed tannic content (HTC), and total flavonoid content (TFC) were spectrophotometrically measured, expressed gallic acid (GAE), tannic acid (TAE), and catechin (CAE) equivalents. Antioxidant capacity was measured by DPPH assay.  Results: The best mandai powder had total phenolic content of 348.8±55.6 mg GAE kg -1, HTC of 143.8±9.3 mg TAE kg -1, TFC of 17.5±1.3 mg CAE kg -1, antioxidant activity (IC 50) of 56.96 ppm, ash content of 4.0±0.7%, pH value of 5.0±0.8, and yield of 9.3±0.8%. There was a strong correlation between TPC, HTC, TFC, and the antioxidant activity. Conclusions: Drying temperature affected all observed parameters but not ash and pH. Temperature of 45°C emerged as the best treatment to produce mandai powder from L. casei-inoculated mandai cempedak fermentation. The antioxidant activity of mandai cempedak was contributed by the phenolic components.

‘Cempedak’ ( Artocarpus integer ) is an aromatic fruit that looks similar to jackfruit. Response surface methodology was used to optimize the spray-drying process to produce ‘cempedak’ fruit powder, with inlet air temperature (140–180 °C) and maltodextrin (DE 10) concentrations (5–15% w/w) as independent variables. Statistical analysis revealed that these variables significantly affected process yield, moisture content, water activity, hygroscopicity, L * value of powder and carotenoid content. Inlet air temperature had a negative correlation with all significant variables. At the same time, maltodextrin concentration was found to be positively correlated with all significant powder variables except process yield and moisture content. The recommended optimum spray-drying conditions for drying ‘cempedak’ juice were inlet air temperature and maltodextrin concentration of 160 °C and 15% (w/w), respectively. Under optimized spray-drying conditions, the yield of ‘cempedak’ powder was 60.5%, with moisture content, water activity, hygroscopicity and carotenoid content of 6.07%, 0.22, 25.8 g/100 g and 1.00 mg/g, respectively.

Nephrotoxicity is a condition characterized by a decline in kidney function due to the toxic effects of medications and substances, such as the nephrotoxic antibiotic gentamicin. Artocarpus champeden is a traditional medicinal plant that is commonly found in Indonesia. This study aims to evaluate the potential of the ethyl acetate fraction of Artocarpus champeden leaves (FEC) in improving kidney function in an animal model of nephrotoxicity induced by gentamicin and piroxicam. Three groups of animals were treated with FEC at dosages of 125, 250, and 500 mg/kg BW orally for four weeks following induction with 100 mg/kg gentamicin (Gen) intraperitoneally and 3.6 mg/kg piroxicam (Prx) orally. The nephroprotective effect of FEC was compared with the NaCMC (5%), ketosteril (55 mg/kg), and untreated groups as the normal, positive, and negative controls, respectively. The kidney biochemical examinations and histopathological analysis were conducted on the last day. Compared to the negative control group, FEC-treated animals showed significant improvement in kidney function, correlating with increasing doses. The test group also exhibited increased creatinine clearance and improved cell structure, comparable to samples treated with ketosteril. The nephroprotective effect of FEC is likely attributed to its flavonoid content, acting through antioxidant and anti-inflammatory mechanisms. The ethyl acetate fraction of A. champeden leaves demonstrated nephroprotective activity and has potential as a therapeutic agent for kidney damage and hypertension from natural sources.

BackgroundGolovinomyces cichoracearum (DC.) is the main pathogen for tobacco powdery mildew fungus disease. Its outbreaks often result in severe harvest losses for the yield and quality of tobacco. Artocarpus champeden is rich in prenylated flavonoids, which are important for the plant’s defensive strategies. With the aim of continuously exploring bioactive natural metabolites for agricultural chemicals, the chemical investigations on the twigs of A. champeden were carried out.ResultsSix new (1–6) and five known (7–11) prenylated flavonoids were isolated. Compound 1 is the first example of flavone whose prenylated side-chain is converted into an unusual 1H-pyrrol-2-yl functional group. Compounds 2 and 3 are rare flavones bearing a 4-methylfuran-2-yl moiety. The frameworks of the above three flavones are reported in natural products for the first time. Interestingly, compound 1 showed high anti-G. cichoracearum activity with an inhibition rate of 88.3% ± 6.2. This rate is higher than that of the positive control (with an inhibition rate of 81.5% ± 6.3) compared to the negative control, compounds 2–11 also showed potential activities with inhibition rates in the range of 50.9%–72.0%. In addition, the mechanistic studies on 1 revealed that it has a potent direct effect on conidiospores of G. cichoracearum and induces systemic acquired resistance for tobacco plants, which may be the reasons for its significant effects against G. cichoracearum.ConclusionsPowdery mildew is a fungal disease harmful to tobacco. Flavonoids have been identified as the sources of promising antifungal agents. For prenylated flavonoids, the combination of a flavonoid skeleton with prenylated side-chain can give the resultant more potential for biological activities. The successful isolation and structure identification of the above prenylated flavonoids provide new materials for the screening of powdery mildew inhibitors, and also contribute to the improved utilization of A. champeden.

'Cempedak' (Artocarpus integer L.) is an aromatic exotic tropical fruit that can be widely found in Malaysia during season. The pulp yield and several physicochemical properties of five varietes of 'cempedak' (CH27, CH28, CH29, CH30 and CH33) were determined. The latter included total soluble solids, titratable acidity, pH, color, organic acids, sugars and carotenoid contents. Sensory evaluation of the five 'cempedak' varieties was conducted using Hedonic test, in which the assessed attributes include color, taste, texture and overall acceptability. Results indicate that CH33 yield the highest percentage (35.8%) of edible portion (fruit pulp), while CH27 shows the highest tiratable acidity (0.52%). CH30 had the lowest L· value (52.41), and highest intensity of color in terms of redness (32.45) and yellowness (65.27) values. All 'cempedak' varieties were highest in sucrose content (12.28-20.02 g/100 gFW) compared to fructose (5.70-6.72 g/100 gFW) and glucose (4.94-5.52 g/100 gFW), while malic acid (0.430.70%) was the highest organic acid as compared to citric acid (0.24-0.60%) and succinic acid (0.20-0.33%). All the 'cempedak' varieties studied have high content of a-carotene (2.30-45.27 pg/100 gFW), followed by ß-carotene (2.30-12.23 pg/100 gFW), with CH28 having the highest content. From the five varieties of 'cempedak' fruit examined, it was found that CH28 ranked the highest in terms of sensory properties, namely taste, texture and overall acceptability.

The potential of mandai cempedak (Artocarpus champeden) powder to be mixed with other abundant raw materials such as oyster mushroom (Pleurotus ostreatus) as a flavoring ingredient is an exciting thing to study as a unique flavor source for the archipelago. This study aims to observe panelist acceptance, proximate characteristics of amino acid, volatile compounds, and color profiles on five mixed formulas of fermented cempedak (Artocarpus champeden) and oyster mushroom (Pleurotus ostreatus) seasoning. The five seasoning formulas combine 30–70% flavored mushroom powder and 30–70% mandai cempedak powder with control of commercial mushroom powder and pure mandai powder. Hedonic quality assessment on seasoning samples of flavored mushroom powder and mandai cempedak powder played a more critical role in the acceptance of the final product, with a slightly reddish yellow color tendency with a paleness level of around 66–67%. Seasoning samples had a savory taste with dominant amino acid profiles of ileusine (1.46%, w/w), glutamate (1.37%), methionine (0.82%), and aspartic acid (0.72%). All seasoning formulations of flavored mushroom and mandai cempedak powder have a moisture content of 8.4–10.9%, total protein 7.0–9.0%, soluble protein 2.4–3.5%, ash content 4.5–19.2%, fat content 2.3–4.5%, carbohydrates 62.7–79.4%, and the solubility is 31.0–89.4%. The dominant volatile compounds in seasoning are heptanone, dodecoxyethanol, and etradecyloxyethanol with pleasant aroma profiles, pungent fruity, green, citrus, and herbal. In conclusion, mandai cempedak powder to be mixed with other abundant raw materials such as oyster mushroom (Pleurotus ostreatus) can be used as a typical Indonesian flavor ingredient with unique characteristics in terms of its amino acid content, volatile compounds, and essential oils.

Premise of the Study Underutilized crops and their wild relatives are important resources for crop improvement and food security. Cempedak [Artocarpus integer (Thunb). Merr.] is a significant crop in Malaysia but underutilized elsewhere. Here we performed molecular characterization of cempedak and its putative wild relative bangkong (Artocarpus integer (Thunb). Merr. var. silvestris Corner) to address questions regarding the origin and diversity of cempedak. Methods Using data from 12 microsatellite loci, we assessed the genetic diversity and genetic/geographic structure for 353 cempedak and 175 bangkong accessions from Malaysia and neighboring countries and employed clonal analysis to characterize cempedak cultivars. We conducted haplotype network analyses on the trnH‐psbA region in a subset of these samples. We also analyzed key vegetative characters that reportedly differentiate cempedak and bangkong. Key Results We show that cempedak and bangkong are sister taxa and distinct genetically and morphologically, but the directionality of domestication origin is unclear. Genetic diversity was generally higher in bangkong than in cempedak. We found a distinct genetic cluster for cempedak from Borneo as compared to cempedak from Peninsular Malaysia. Finally, cempedak cultivars with the same names did not always share the same genetic fingerprint. Conclusions Cempedak origins are complex, with likely admixture and hybridization with bangkong, warranting further investigation. We provide a baseline of genetic diversity of cempedak and bangkong in Malaysia and found that germplasm collections in Malaysia represent diverse coverage of the four cempedak genetic clusters detected.

Deforestation is rapidly transforming primary forests across the tropics into human-dominated landscapes. Consequently, conservationists need to understand how different taxa respond and adapt to these changes in order to develop appropriate management strategies. Our two year study seeks to determine how wild Sumatran orangutans (Pongo abelii) adapt to living in an isolated agroforest landscape by investigating the sex of crop-raiders related to population demographics, and their temporal variations in feeding behaviour and dietary composition. From focal animal sampling we found that nine identified females raided cultivated fruits more than the four males. Seasonal adaptations were shown through orangutan feeding habits that shifted from being predominantly fruit-based (56% of the total feeding time, then 22% on bark) to the fallback food of bark (44%, then 35% on fruits), when key cultivated resources such as jackfruit (Artocarpus integer), were unavailable. Cultivated fruits were mostly consumed in the afternoon and evening, when farmers had returned home. The finding that females take greater crop-raiding risks than males differs from previous human-primate conflict studies, probably because of the low risks associated (as farmers rarely retaliated) and low intraspecific competition between males. Thus, the behavioral ecology of orangutans living in this human-dominated landscape differs markedly from that in primary forest, where orangutans have a strictly wild food diet, even where primary rainforests directly borders farmland. The importance of wild food availability was clearly illustrated in this study with 21% of the total orangutan feeding time being allocated to feeding on cultivated fruits. As forests are increasingly converted to cultivation, humans and orangutans are predicted to come into conflict more frequently. This study reveals orangutan adaptations for coexisting with humans, e.g. changes in temporal foraging patterns, which should be used for guiding the development of specific human-wildlife conflict mitigation strategies to lessen future crop-raiding and conflicts.