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"BIOMASS FUELS"
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Our energy future : introduction to renewable energy and biofuels
\"Our Energy Future is an introductory textbook for a college course in energy production, alternative and renewable fuels, and related issues involved in building a sustainable energy future. Our society is consuming energy at an alarming rate as trends in energy consumption continue to rise. Jones and Mayfield explore the creation and history of fossil fuels, their impact on the environment, and how they have become critical to our society. They warn that continuing fuel-usage patterns could permanently damage our environment. Jones and Mayfield also outline how the adoption of sustainable biofuels will be key to our future energy stability. They discuss a number of renewable energy options, and then discuss different biofuel feedstocks and their potential as replacements for petroleum-based products. This book emphasizes the importance of continued scientific, agricultural, and engineering development, while outlining the political and environmental challenges that are coupled with a complete shift from fossil fuels to renewable energy and biomass. Our Energy Future is an excellent, accessible resource for undergraduate students studying biofuels and bioenergy.\"--Provided by publisher.
Book
Measurement of Black Carbon Absorption Coefficients Using an Aethalometer and Their Association with Visibility
Black carbon (BC) is a pollutant aerosol affecting climate and human health. Light absorption coefficients of black carbon (Babs) were measured using an aethalometer model AE33 at wavelengths 370, 470,520,660,880, and 950 nm. Babs for the seven wavelengths at seven sites in Jordan fluctuated with time and peaked at rush hours. The daily average values for all sites were inversely proportional to the wavelength. The average daily visibility values in the seven Jordan sites varied between 72 km and 211km. In the Irbid site, the daily average visibility values for 7-13 Nov. 2021 varied between 43 km and 107 km. BC varied from hour to hour and from day to day. The daily average values of BC in Irbid for the period of 7 -13 Nov. 2021 varied between 2.24 μg.m-3 and 4.66 μg.m-3. BC peaked at the rush hour and had the lowest values on Friday. About 90% of the measured BC was from fossil fuel sources and 10% from biomass-burning sources.
Journal Article
Development of a thermophilic coculture for corn fiber conversion to ethanol
The fiber in corn kernels, currently unutilized in the corn to ethanol process, represents an opportunity for introduction of cellulose conversion technology. We report here that Clostridium thermocellum can solubilize over 90% of the carbohydrate in autoclaved corn fiber, including its hemicellulose component glucuronoarabinoxylan (GAX). However, Thermoanaerobacterium thermosaccharolyticum or several other described hemicellulose-fermenting thermophilic bacteria can only partially utilize this GAX. We describe the isolation of a previously undescribed organism, Herbinix spp. strain LL1355, from a thermophilic microbiome that can consume 85% of the recalcitrant GAX. We sequence its genome, and based on structural analysis of the GAX, identify six enzymes that hydrolyze GAX linkages. Combinations of up to four enzymes are successfully expressed in T. thermosaccharolyticum. Supplementation with these enzymes allows T. thermosaccharolyticum to consume 78% of the GAX compared to 53% by the parent strain and increases ethanol yield from corn fiber by 24%.
Journal Article
Elephant Clan Optimization for Scenario‐Based Fuel‐Constrained Heat and Power Scheduling for Remote Microgrid
This manuscript proposes elephant clan optimization (ECO) to address heat and electric power scheduling in remote microgrids (MGs) under three distinct scenarios, considering fuel constraints. ECO is a population‐based approach influenced by the behavior and social structure of elephants. The MG consists of wind turbine generators (WTGs), biomass‐fuel‐fired micro‐cogeneration (BMC) units, diesel generators (DGs), battery energy storage systems (BESS), solar micro‐cogeneration (SMC) units, and plug‐in electric vehicles (PEVs). BMC units and SMC units are alternately integrated into the MG. Numerical results of a standard system are contrasted with those derived from a hierarchical particle swarm optimizer with time‐varying acceleration coefficients (HPSO‐TVAC), as well as gray wolf optimization (GWO). The analysis demonstrates that ECO offers an improved solution. This manuscript proposes ECO (elephant clan optimization) to address heat and electric power scheduling in remote MG (microgrids) under three distinct scenarios, considering fuel constraints. ECO is a population‐based approach influenced by the behavior and social structure of elephants. The microgrid (MG) constitutes WTGs (wind turbine generators), BMC (biomass‐fuel‐fired micro‐cogeneration) units, DGs (diesel generators), BESS (battery energy storage systems), SMC (solar micro‐cogeneration) units, and PEVs (plug‐in electric vehicles). BMC units and SMC units are alternately integrated into MG. Numerical results of a standard system contrasted with those derived from HPSO‐TVAC (hierarchical particle swarm optimizer with time‐varying acceleration coefficients) as well as GWO (gray wolf optimization). The analysis demonstrates ECO offers an improved solution.
Journal Article
High Flux Through the Oxidative Pentose Phosphate Pathway Lowers Efficiency in Developing Camelina Seeds
Many seeds are green during development, and light has been shown to play a role in the efficiency with which maternally supplied substrates are converted into storage compounds. However, the effects of light on the fluxes through central metabolism that determine this efficiency are poorly understood. Here, we used metabolic flux analysis to determine the effects of light on central metabolism in developing embryos of false flax (Camelina sativa). Metabolic efficiency in C. sativa is of interest because, despite its growing importance as a model oilseed and engineering target and its potential as a biofuel crop, its yields are lower than other major oilseed species. Culture conditions under which steady-state growth and composition of developing embryos match those in planta were used to quantify substrate uptake and respiration rates. The carbon conversion efficiency (CCE) was 21% 6 3% in the dark and 42% 6 4% under high light. Under physiological illumination, the CCE (32% 6 2%) was substantially lower than in green and nongreen oilseeds studied previously. 13C and 14C isotopic labeling experiments were used together with computer-aided modeling to map fluxes through central metabolism. Fluxes through the oxidative pentose phosphate pathway (OPPP) were the principal source of CO2 production and strongly negatively correlated with CCE across light levels. OPPP fluxes were greatly in excess of demand for NAD(P)H for biosynthesis and larger than those measured in other systems. Excess reductant appears to be dissipated via cyanide-insensitive respiration. OPPP enzymes therefore represent a potential target for increasing efficiency and yield in C. sativa.
Journal Article
Assembly and seasonality of core phyllosphere microbiota on perennial biofuel crops
Perennial grasses are promising feedstocks for biofuel production, with potential for leveraging their native microbiomes to increase their productivity and resilience to environmental stress. Here, we characterize the 16S rRNA gene diversity and seasonal assembly of bacterial and archaeal microbiomes of two perennial cellulosic feedstocks, switchgrass (Panicum virgatum L.) and miscanthus (Miscanthus x giganteus). We sample leaves and soil every three weeks from pre-emergence through senescence for two consecutive switchgrass growing seasons and one miscanthus season, and identify core leaf taxa based on occupancy. Virtually all leaf taxa are also detected in soil; source-sink modeling shows non-random, ecological filtering by the leaf, suggesting that soil is an important reservoir of phyllosphere diversity. Core leaf taxa include early, mid, and late season groups that were consistent across years and crops. This consistency in leaf microbiome dynamics and core members is promising for microbiome manipulation or management to support crop production.
Journal Article
Potential for Genetic Improvement of Sugarcane as a Source of Biomass for Biofuels
Sugarcane (Saccharum spp. hybrids) has great potential as a major feedstock for biofuel production worldwide. It is considered among the best options for producing biofuels today due to an exceptional biomass production capacity, high carbohydrate (sugar + fiber) content, and a favorable energy input/output ratio. To maximize the conversion of sugarcane biomass into biofuels, it is imperative to generate improved sugarcane varieties with better biomass degradability. However, unlike many diploid plants, where genetic tools are well developed, biotechnological improvement is hindered in sugarcane by our current limited understanding of the large and complex genome. Therefore, understanding the genetics of the key biofuel traits in sugarcane and optimization of sugarcane biomass composition will advance efficient conversion of sugarcane biomass into fermentable sugars for biofuel production. The large existing phenotypic variation in Saccharum germplasm and the availability of the current genomics technologies will allow biofuel traits to be characterized, the genetic basis of critical differences in biomass composition to be determined, and targets for improvement of sugarcane for biofuels to be established. Emerging options for genetic improvement of sugarcane for the use as a bioenergy crop are reviewed. This will better define the targets for potential genetic manipulation of sugarcane biomass composition for biofuels.
Journal Article
Understanding the Effects of Ash Content on Various Pretreatment Technologies for the Bioconversion of Corn Stover
The study aimed to examine the effects of adding biomass ash on the biochemical processes involved in fermentable sugar production. Corn stover was pretreated using several methods—hot water, dilute acid, alkaline, γ-valerolactone, and ionic liquid methods, each examined with ash loadings of 7.18% and 21.07%. The findings demonstrated that increased ash content adversely affected both pretreatment and enzymatic hydrolysis. Specifically, the total sugar yield was 3 to 16% lower at the higher ash content across all pretreatment methods, and up to 4.01% lower during enzymatic hydrolysis. For acidic pretreatment, the sugar yield decreased as ash content increased. In contrast, ash content had a lesser impact on alkaline pretreatment compared to acidic pretreatment. For example, using corn stover with an ash content as high as 22.65% resulted in only a 2.90% decrease in total sugar yield compared to corn stover without added ash. Further, the primary reasons for the reduced sugar yield in higher ash biomass during acidic pretreatments were likely the neutralizing effect of the ash and decreased acid access to the substrates. During enzymatic hydrolysis, ash reduced the sugar yield by limiting enzyme access to cellulose.
Journal Article
Lignin valorization through integrated biological funneling and chemical catalysis
Lignin is an energy-dense, heterogeneous polymer comprised of phenylpropanoid monomers used by plants for structure, water transport, and defense, and it is the second most abundant biopolymer on Earth after cellulose. In production of fuels and chemicals from biomass, lignin is typically underused as a feedstock and burned for process heat because its inherent heterogeneity and recalcitrance make it difficult to selectively valorize. In nature, however, some organisms have evolved metabolic pathways that enable the utilization of lignin-derived aromatic molecules as carbon sources. Aromatic catabolism typically occurs via upper pathways that act as a “biological funnel” to convert heterogeneous substrates to central intermediates, such as protocatechuate or catechol. These intermediates undergo ring cleavage and are further converted via the β-ketoadipate pathway to central carbon metabolism. Here, we use a natural aromatic-catabolizing organism, Pseudomonas putida KT2440, to demonstrate that these aromatic metabolic pathways can be used to convert both aromatic model compounds and heterogeneous, lignin-enriched streams derived from pilot-scale biomass pretreatment into medium chain-length polyhydroxyalkanoates (mcl -PHAs). mcl -PHAs were then isolated from the cells and demonstrated to be similar in physicochemical properties to conventional carbohydrate-derived mcl -PHAs, which have applications as bioplastics. In a further demonstration of their utility, mcl -PHAs were catalytically converted to both chemical precursors and fuel-range hydrocarbons. Overall, this work demonstrates that the use of aromatic catabolic pathways enables an approach to valorize lignin by overcoming its inherent heterogeneity to produce fuels, chemicals, and materials.
Journal Article
A unique Co@CoO catalyst for hydrogenolysis of biomass-derived 5-hydroxymethylfurfural to 2,5-dimethylfuran
The development of precious-metal-free catalysts to promote the sustainable production of fuels and chemicals from biomass remains an important and challenging target. Here, we report the efficient hydrogenolysis of biomass-derived 5-hydroxymethylfurfural to 2,5-dimethylfuran over a unique core-shell structured catalyst, Co@CoO that affords the highest productivity among all catalysts, including noble-metal-based catalysts, reported to date. Surprisingly, we find that the catalytically active sites reside on the shell of CoO with oxygen vacancies rather than the metallic Co. The combination of various spectroscopic experiments and computational modelling reveals that the CoO shell incorporating oxygen vacancies not only drives the heterolytic cleavage, but also the homolytic cleavage of H
2
to yield more active H
δ−
species, resulting in the exceptional catalytic activity. Co@CoO also exhibits excellent activity toward the direct hydrodeoxygenation of lignin model compounds. This study unlocks, for the first time, the potential of simple metal-oxide-based catalysts for the hydrodeoxygenation of renewable biomass to chemical feedstocks.
The development of precious-metal-free catalysts to promote the sustainable production of fuels and chemicals from biomass challenging. Here the authors report a unique core-shell structured Co@CoO catalyst which exhibits excellent performance in the hydrogenolysis of biomass-derived compounds.
Journal Article