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"Bacteriology Laboratory manuals"
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Basic laboratory procedures in clinical bacteriology
This manual is a practical guide, for use by laboratory workers in health centres and district hospitals, to the procedures to be followed in obtaining specimens, isolating and identifying bacteria, and assessing their resistance to antibiotics. It covers bacteriological investigation of blood, cerebrospinal fluid, urine, stool, sputum, pharyngeal and genital specimens, and purulent exudates. Particular attention is given to the need for quality control of all laboratory procedures. A list of media and reagents needed for the isolation and identification of the most common bacterial pathogens is included, together with an indication of their relative importance for the intermediary laboratory. This list is intended for adaptation to local circumstances. This second edition has been updated in many areas, including a greatly enhanced section on stool specimens and a new section on serological tests.
eBook
Practical Medical Microbiology for Clinicians
Infectious diseases constitute a major portion of illnesses worldwide, and microbiology is a main pillar of clinical infectious disease practice. Knowledge of viruses, bacteria, fungi, and parasites is integral to practice in clinical infectious disease. Practical Medical Microbiology is an invaluable reference for medical microbiology instructors. Drs. Berkowitz and Jerris are experienced teachers in the fields of infectious diseases and microbiology respectively, and provide expert insight into microorganisms that affect patients, how organisms are related to each other, and how they are isolated and identified in the microbiology laboratory. The text also is designed to provide clinicians the knowledge they need to facilitate communication with the microbiologist in their laboratory. The text takes a systematic approach to medical microbiology, describing taxonomy of human pathogens and consideration of organisms within specific taxonomic groups. The text tackles main clinical infections caused by different organisms, and supplements these descriptions with clinical case studies, in order to demonstrate the effects of various organisms. Practical Medical Microbiology is an invaluable resource for students, teachers, and researchers studying clinical microbiology, medical microbiology, infectious diseases, and virology.
eBook
Vaccination against serogroup B Neisseria meningitidis: Perceptions and attitudes of parents
•The spontaneous acceptance of serogroup B MID vaccination is unsatisfactory.•The lack of hindsight and adverse effects fear are the main obstacles to vaccination.•After information about the disease and vaccine, few parents are completely resistant.•GPs and paediatricians may play a crucial role in implementing vaccine acceptance.
A vaccine against serogroup B Neisseria meningitidis, major cause of bacterial meningitis in children and adults, has recently been developed. In a context of an increasing parental mistrust against vaccinations, understanding the reason for their choices is crucial in order to improve immunization coverage. Our study aimed at evaluating parental attitudes and perceptions towards serogroup B meningococcal invasive disease vaccination.
A prospective observational study was conducted in different French independent-practice medical offices (general practitioners and paediatricians) and nurseries between May 1 and December 31, 2013, using a questionnaire distributed in electronic and paper forms to parents having at least one child between the ages of 2 months and 16 years old.
1270 parents were included, of whom 671 (52.8%) spontaneously stated to be in favour of this vaccination. Their choice was mainly justified by the severity of the disease (63.8%) and the desire to protect their child (51.7%). In multivariate analysis, the young age of parents (OR 0.949 per additional year; p<10−3), the history of vaccination against serogroup C meningococcal invasive diseases (OR 6.755; p<10−3), and the prior knowledge of the vaccine (OR 2.081; p=0.001) were associated with vaccination acceptance. The main reasons for refusal were the lack of hindsight on this new vaccine (50.6%) and the fear of side effects (45.5%). After objective information on the disease and the vaccine, only 6.3% of the entire responding population would refuse to consider vaccination.
The spontaneous acceptance rate of vaccination against serogroup B meningococcal invasive disease is insufficient. However, after objective information by their physician or public health authorities, only a few parents would in the end be completely resistant.
Journal Article
Highly Variable Mutation Rates in Commensal and Pathogenic Escherichia coli
Matic et al studied mutation rates among different Escherichia coli populations isolated from distinct environments. Defects were found in methyl-directed mismatch repair (MMR) genes, as well as defects in all the phylogenetic groups and independently of the commensal or pathogenic nature of the strains.
Journal Article