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"Botany projects."
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Experimenting with plants : science projects
\"Learn the science behind plant physiology, reproduction and growth\"-- Provided by publisher.
Book
Basin-scale effects of small hydropower on biodiversity dynamics
Construction of small hydropower plants (<10 megawatts) is booming worldwide, exacerbating ongoing habitat fragmentation and degradation, and further fueling biodiversity loss. A systematic approach for selecting hydropower sites within river networks may help to minimize the detrimental effects of small hydropower on biodiversity. In addition, a better understanding of reachand basin-scale impacts is key for designing planning tools. We synthesize the available information about (1) reach-scale and (2) basin-scale impacts of small hydropower plants on biodiversity and ecosystem function, and (3) interactions with other anthropogenic Stressors. We then discuss state-of-the-art, spatially explicit planning tools and suggest how improved knowledge of the ecological and evolutionary impacts of hydropower can be incorporated into project development. Such tools can be used to balance the benefits of hydropower production with the maintenance of ecosystem services and biodiversity conservation. Adequate planning tools that consider basin-scale effects and interactions with other Stressors, such as climate change, can maximize longterm conservation.
Journal Article
Experiments with plants
\"Explore the world of plants and seeds with ... real-world examples and ... step-by-step experiments\"--Page 4 of cover.
Book
Habitat restoration benefits wild bees: A meta-analysis
1. Pollinator conservation is of increasing interest in the light of managed honeybee (Apis mellifera) declines, and declines in some species of wild bees. Much work has gone into understanding the effects of habitat enhancements in agricultural systems on wild bee abundance, richness and pollination services. However, the effects of ecological restoration targeting \"natural\" ecological endpoints (e.g. restoring former agricultural fields to historic vegetation types or improving degraded natural lands) on wild bees have received relatively little attention, despite their potential importance for countering habitat loss. 2. We conducted a meta-analysis to evaluate the effects of ecological restoration on wild bee abundance and richness, focusing on unmanaged bee communities in lands restored and managed to increase habitat availability and quality. Specifically, we assessed bee abundance and/or richness across studies comparing restored vs. unrestored treatments and studies investigating effects of specific habitat restoration techniques, such as burning, grazing, invasive plant removal and seeding. 3. We analysed 28 studies that met our selection criteria: these represented 11 habitat types and 7 restoration techniques. Nearly all restorations associated with these studies were performed without explicit consideration of habitat needs for bees or other pollinators. The majority of restorations targeted plant community goals, which could potentially have ancillary benefits for bees. 4. Restoration had overall positive effects on wild bee abundance and richness across multiple habitat types. Specific restoration actions, tested independently, also tended to have positive effects on wild bee richness and abundance. 5. Synthesis and applications. We found strong evidence that ecological restoration advances wild bee conservation. This is important given that habitat loss is recognized as a leading factor in pollinator decline. Pollinator responses to land management are rarely evaluated in non-agricultural settings and so support for wild bees may be an underappreciated benefit of botanically focused management. Future restoration projects that explicitly consider the needs of wild bees could be more effective at providing nesting, foraging and other habitat resources. We encourage land managers to design and evaluate restoration projects with the habitat needs of wild bee species in mind.
Journal Article
Experiments with plants
\"Explore the world of plants and seeds with clear text, real-world examples and fun, safe step-by-step experiments.\"--Back cover.
Book
Untangling the hedge: Genetic diversity in clonally and sexually transmitted genomes of European wild roses, Rosa L
While European wild roses are abundant and widely distributed, their morphological taxonomy is complicated and ambiguous. In particular, the polyploid Rosa section Caninae (dogroses) is characterised by its unusual meiosis, causing simultaneous clonal and sexual transmission of sub-genomes. This hemisexual reproduction, which often co-occurs with vegetative reproduction, defies the standard definition of species boundaries. We analysed seven highly polymorphic microsatellite loci, scored for over 2 600 Rosa samples of differing ploidy, collected across Europe within three independent research projects. Based on their morphology, these samples had been identified as belonging to 21 dogrose and five other native rose species. We quantified the degree of clonality within species and at individual sampling sites. We then compared the genetic structure within our data to current rose morpho-systematics and searched for hemisexually co-inherited sets of alleles at individual loci. We found considerably fewer copies of identical multi-locus genotypes in dogroses than in roses with regular meiosis, with some variation recorded among species. While clonality showed no detectable geographic pattern, some genotypes appeared to be more widespread. Microsatellite data confirmed the current classification of subsections, but they did not support most of the generally accepted dogrose microspecies. Under canina meiosis, we found co-inherited sets of alleles as expected, but could not distinguish between sexually and clonally inherited sub-genomes, with only some of the detected allele combinations being lineage-specific.
Journal Article
The platform GrowScreen-Agar enables identification of phenotypic diversity in root and shoot growth traits of agar grown plants
Background
Root system architecture and especially its plasticity in acclimation to variable environments play a crucial role in the ability of plants to explore and acquire efficiently soil resources and ensure plant productivity. Non-destructive measurement methods are indispensable to quantify dynamic growth traits. For closing the phenotyping gap, we have developed an automated phenotyping platform,
GrowScreen
-
Agar
, for non-destructive characterization of root and shoot traits of plants grown in transparent agar medium.
Results
The phenotyping system is capable to phenotype root systems and correlate them to whole plant development of up to 280
Arabidopsis
plants within 15 min. The potential of the platform has been demonstrated by quantifying phenotypic differences within 78
Arabidopsis
accessions from the 1001 genomes project. The chosen concept ‘plant-to-sensor’ is based on transporting plants to the imaging position, which allows for flexible experimental size and design. As transporting causes mechanical vibrations of plants, we have validated that daily imaging, and consequently, moving plants has negligible influence on plant development. Plants are cultivated in square Petri dishes modified to allow the shoot to grow in the ambient air while the roots grow inside the Petri dish filled with agar. Because it is common practice in the scientific community to grow
Arabidopsis
plants completely enclosed in Petri dishes, we compared development of plants that had the shoot inside with that of plants that had the shoot outside the plate. Roots of plants grown completely inside the Petri dish grew 58% slower, produced a 1.8 times higher lateral root density and showed an etiolated shoot whereas plants whose shoot grew outside the plate formed a rosette. In addition, the setup with the shoot growing outside the plate offers the unique option to accurately measure both, leaf and root traits, non-destructively, and treat roots and shoots separately.
Conclusions
Because the
GrowScreen
-
Agar
system can be moved from one growth chamber to another, plants can be phenotyped under a wide range of environmental conditions including future climate scenarios. In combination with a measurement throughput enabling phenotyping a large set of mutants or accessions, the platform will contribute to the identification of key genes.
Journal Article
Improving primary students' collaborative problem solving competency in project-based science learning with productive failure instructional design in a seamless learning environment
The paper reports on an empirical study adopting a mixed research method, aiming at improving primary students' collaborative problem solving competency in project-based learning with productive failure (PF) instructional design in a seamless learning environment. Two Grade Six classes participated in a project-based learning of \"Plant Adaptations\". In Class 1 with 27 students, the project-based learning was conducted with PF instructional design; in Class 2 with 26 students, the project-based learning was conducted without PF instructional design. The learning activities spanned across farm, class, home and online spaces supported by mobile devices. Data collection includes various students' created artifacts in groups in the inquiry process, student reflections, student focus group interviews and pre- and post-domain tests. Both qualitative and quantitative data analysis methods were employed. The research findings show that compared to Class 2, the students in Class 1 gained deeper understanding of conceptual knowledge and produced better group artifacts in collaborative problem-solving quality than those in Class 2; and the students in Class 1 were more positive in facing the challenges in their project-based learning process, and developed a sense of ownership of their learning. The findings imply that PF instructional design is conducive to developing primary students' collaborative solving competency in science learning in a seamless learning environment.
Journal Article
Leaf-transcriptome profiles of phoebe bournei provide insights into temporal drought stress responses
Phoebe bournei (Hemsl.) Yang is used as a commercial wood in China and is enlisted as a near-threatened species. Prolonged droughts pose a serious threat to young seedlings (1-2 years old). A transcriptome sequencing approach, together with the measurement of growth parameters and biochemical analyses were used to understand P. bournei’s drought responses on 15d, 30d, and 45d of drought stress treatment. The stem and root dry weights decreased significantly with drought stress duration. Activities of antioxidative enzymes i.e., peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) increased significantly with the increase in drought stress duration. A total of 13,274, 15,648, and 9,949 genes were differentially expressed in CKvs15d, CKvs30d, and CKvs45d, respectively. The differential expression analyses showed that photosystem I and II underwent structural changes, chlorophyll biosynthesis, and photosynthesis were reduced. The genes annotated as POD, SOD, and CAT were upregulated in drought-treated leaves as compared to control. Additionally, plant-hormone signal transduction, MAPK signaling-plant, phenylpropanoid biosynthesis, flavonoid biosynthesis, and starch and sucrose metabolism pathways showed large-scale expression changes in major genes. We also found that members of 25 transcription factor families were differentially expressed. Our study presents and discusses these transcriptome signatures. Overall, our findings represent key data for breeding towards drought stress tolerance in P. bournei .
Journal Article
Assessment of the state of the art of technologies for the processing of digestate residue from anaerobic digesters
Anaerobic digestion is widely used as an important source of renewable energy. With the increasing number and capacity of biogas plants also, adequate treatment technologies for whole digestate – the residue from anaerobic digestion – are gaining attention. In this study the state of the art of digestate processing is analysed, and currently used treatment schemes and the various technological processes involved are evaluated. The study combines data and experiences from existing large-scale digestate processing facilities in Austria, Germany, Switzerland and Italy, as well as know-how from technology providers and relevant research projects. However, the field of digestate processing is still quite new and little detailed information about the performance of different technologies at industrial scale is available. Digestate processing is gaining importance since digestate utilisation can become an important bottleneck when increasing biogas production. In addition, the production of renewable fertiliser from digestate is increasingly of interest to replace fossil fertilisers. This study is the first profound attempt to establish an assessment of the state-of-the-art technologies in use.
Journal Article