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Pea protein dry-fractionated (PDF), pea protein isolated (PIs), soy protein isolated (SIs) and oat protein (OP) were combined in four mixes (PDF_OP, PIs_OP, PDF_PIs_OP, SIs_OP) and extruded to produce meat analogues. The ingredients strongly influenced the process conditions and the use of PDF required higher specific mechanical energy and screw speed to create fibrous texture compared to PIs and SIs. PDF can be conveniently used to produce meat analogues with a protein content of 55 g 100 g−1, which is exploitable in meat-alternatives formulation. PDF-based meat analogues showed lower hardness (13.55–18.33 N) than those produced from PIs and SIs (nearly 27 N), probably due to a more porous structure given by the natural presence of carbohydrates in the dry-fractionated ingredient. PDF_OP and PIs_PDF_OP showed a significantly lower water absorption capacity than PIs OP and SIs_OP, whereas pea-based extrudates showed high oil absorption capacity, which could be convenient to facilitate the inclusion of oil and fat in the final formulation. The sensory evaluation highlighted an intense odor and taste profile of PDF_OP, whereas the extrudates produced by protein isolates had more neutral sensory characteristics. Overall, the use of dry-fractionated protein supports the strategies to efficiently produce clean-labeled and sustainable plant-based meat analogues.

ABSTRACT A 9-week feeding trial was conducted to investigate the effects of Isaria cicadae Miquel (I. cicadae) on growth performance and meat quality of giant freshwater prawn (Macrobrachium rosenbergii). The prawns were fed with five isonitrogenous (40.54% crude protein) and isolipid (6.16% crude lipid) diets with different I. cicadae levels (0, 100, 200, 400, and 800 mg Kg-1). Results revealed that I. cicadae significantly increased specific growth ratio (SGR) and protein efficiency ratio (PER), while decreased feed intake (fi) and feed conversion ratio (FCR). Meanwhile, I. cicadae significantly increased plasma ALT activity, urea nitrogen, total protein, albumin and globulin contents, while significantly decreased AST activity in 400 mg Kg-1 group. haemolymph SOD and GSh-PX activity was enhanced significantly in 400 and 800 mg Kg-1 group, respectively, while MDA content was significantly decrease in these two groups. I. cicadae did not affect the muscle cooking loss, but significantly increased the compression loss. Moreover, I. cicadae significantly increased the muscle texture profile including gumminess, hardness and chewiness, and decreased the muscle sensory evaluations including whiteness and succulency. Therefore, 100 mg Kg-1 I. cicadae could improve growth performance and meat quality of giant freshwater prawn, reduce the damage of oxidative stress.

When food writer Richard Cornish was so overcome by the aroma of the roast leg of lamb he had buckled into the passenger seat next to him that he pulled over to the side of the road and tore it apart with his bare hands, he knew he had a problem.He began to examine what it means to eat meat by becoming vegetarian for a year.