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Scanning fluorescence X-ray microscopy lets one non-destructively and quantitatively map the distribution of most biologically important metals in cells and tissues. For studies on large-scale tissues and organs, a spatial resolution of several micrometres is often sufficient; in this case, bending magnets at synchrotron light sources provide abundant X-ray flux. We describe here the use of bending magnet beamline 8-BM-B at the Advanced Photon Source with two distinct microscopy stations: a pre-existing one with Kirkpatrick–Baez (KB) mirror optics for slightly higher throughput and the ability to accommodate samples tens of centimetres across, and a new prototype station with an axially symmetric, single-bounce, capillary optic with slightly less flux, but finer resolution at similar fluence per time. The KB station provides δ res = 10.5 µm spatial resolution at a per-pixel exposure time of t dwell = 100 ms and a fluence per time of 5.8 × 10 7 photons µm −2 s −1 , while the prototype capillary station provides δ res = 6.5 µm at t dwell = 50 ms and a fluence per time of 5.6 × 10 7 photons µm −2 s −1 . We used image power spectral density to estimate the achieved spatial resolution δ res from individually acquired images, with δ res depending on the optic, the fluorescence signal strength of the sample being imaged, and the method used to process raw fluorescence spectral data.

Optical soliton dynamics can cause extreme alteration of the temporal and spectral shape of a propagating light pulse. This occurs at up to kilowatt peak powers in glass-core optical fibres and at the gigawatt level in gas-filled microstructured hollow-core fibres. Here, we demonstrate optical soliton dynamics in large-core hollow capillary fibres. This enables scaling of soliton effects by several orders of magnitude to the multi-millijoule energy and terawatt peak power level. We experimentally demonstrate two key soliton effects. First, we observe self-compression to sub-cycle pulses and infer the creation of sub-femtosecond field waveforms—a route to high-power optical attosecond pulse generation. Second, we efficiently generate continuously tunable high-energy (1–16 μJ) pulses in the vacuum and deep ultraviolet (110 nm to 400 nm) through resonant dispersive-wave emission. These results promise to be the foundation of a new generation of table-top light sources for ultrafast strong-field physics and advanced spectroscopy.Optical soliton dynamics in large-core hollow capillary fibres is demonstrated. The findings enable the scaling of soliton effects by several orders of magnitude to the multi-millijoule energy and terawatt peak power levels, and open up opportunities for new-generation table-top light sources for ultrafast strong-field physics and advanced spectroscopy.

Coherent dispersive wave emission, as an important phenomenon of soliton dynamics, manifests itself in multiple platforms of nonlinear optics from fibre waveguides to integrated photonics. Limited by its resonance nature, efficient generation of coherent dispersive wave with ultra-broad bandwidth has, however, proved difficult to realize. Here, we unveil a new regime of soliton dynamics in which the dispersive wave emission process strongly couples with the splitting dynamics of the driving pulse. High-order dispersion and self-steepening effects, accumulated over soliton self-compression, break the system symmetry, giving rise to high-efficiency generation of coherent dispersive wave in the ultraviolet region. Simultaneously, asymmetric soliton splitting results in the appearance of a temporally-delayed ultrashort pulse with high intensity, overlapping and copropagating with the dispersive wave pulse. Intense cross-phase modulations lead to octave-wide broadening of the dispersive wave spectrum, covering 200–400 nm wavelengths. The highly-coherent, octave-wide ultraviolet spectrum, generated from the simple capillary fibre set-up, is in great demand for time-resolved spectroscopy, ultrafast electron microscopy and frequency metrology applications, and the critical role of the secondary pulse in this process reveals some new opportunities for all-optical control of versatile soliton dynamics. Here the authors propose method for generating octave-spectral-wide ultraviolet pulses in capillary fibres by coupling dispersive-wave radiation with soliton-splitting dynamics, which may trigger advanced applications in ultrafast optics.

Non-arteritic anterior ischemic optic neuropathy (NAION) is the most common cause of non-glaucomatous optic neuropathy in older adults. Optical coherence tomographic angiography (OCT-A) is an emerging, non-invasive method to study the microvasculature of the posterior pole, including the optic nerve head. The goal of this study was to assess the vascular changes in the optic nerve head and peripapillary area associated with NAION using OCT-A. Retrospective comparative case series. We performed OCT-A in 25 eyes (7 acute and 18 non-acute) in 19 patients with NAION. Fellow, unaffected eyes were analyzed for comparison. Patent macro- and microvascular densities were quantified in the papillary and peripapillary regions of unaffected, acutely affected, and non-acutely affected eyes and compared across these groups according to laminar segment and capillary sampling region, and with respect to performance on automated visual field testing. In acutely affected eyes, OCT-A revealed a reduction in the signal from the major retinal vessels and dilation of patent superficial capillaries in the peripapillary area. By contrast, non-acutely affected eyes showed attenuation of patent capillaries. The peripapillary choriocapillaris was obscured by edema in acute cases, but was similar between non-acute and unaffected eyes. The degree of dilation of the superficial microvasculature in the acute phase and attenuation in the non-acute phase each correlated inversely with visual field performance. The region of reduced patent capillary density correlated with the location of visual field defects in 80% of acute cases and 80% of non-acute cases. OCT-A reveals a dynamic shift in the superficial capillary network of the optic nerve head with strong functional correlates in both the acute and non-acute phases of NAION. Further study may validate OCT-A as a useful adjunctive diagnostic tool in the evaluation of ischemic optic neuropathy.

Realizing programmable assembly and reconfiguration of small objects holds promise for technologically-significant applications in such fields as micromechanical systems, biomedical devices, and metamaterials. Although capillary forces have been successfully explored to assemble objects with specific shapes into ordered structures on the liquid surface, reconfiguring these assembled structures on demand remains a challenge. Here we report a strategy, bioinspired by Anurida maritima , to actively reconfigure assembled structures with well-defined selectivity, directionality, robustness, and restorability. This approach, taking advantage of optocapillarity induced by photodeformation of floating liquid crystal polymer actuators, not only achieves programmable and reconfigurable two-dimensional assembly, but also uniquely enables the formation of three-dimensional structures with tunable architectures and topologies across multiple fluid interfaces. This work demonstrates a versatile approach to tailor capillary interaction by optics, as well as a straightforward bottom-up fabrication platform for a wide range of applications. When floating at the air water interface, the shape of objects determines their capillary menisci and mutual interaction. Hu et al. show how dynamically changing shapes of liquid-crystal polymer actuators can be used to achieve optically controlled and reconfigurable assembly.

To assess the effect of image registration and averaging on the visualization and quantification of the radial peripapillary capillary (RPC) network on optical coherence tomography angiography (OCTA). Twenty-two healthy controls were imaged with a commercial OCTA system (AngioVue, Optovue, Inc.). Ten 10x10° scans of the optic disc were obtained, and the most superficial layer (50-μm slab extending from the inner limiting membrane) was extracted for analysis. Rigid registration was achieved using ImageJ, and averaging of each 2 to 10 frames was performed in five ~2x2° regions of interest (ROI) located 1° from the optic disc margin. The ROI were automatically skeletonized. Signal-to-noise ratio (SNR), number of endpoints and mean capillary length from the skeleton, capillary density, and mean intercapillary distance (ICD) were measured for the reference and each averaged ROI. Repeated measures analysis of variance was used to assess statistical significance. Three patients with primary open angle glaucoma were also imaged to compare RPC density to controls. Qualitatively, vessels appeared smoother and closer to histologic descriptions with increasing number of averaged frames. Quantitatively, number of endpoints decreased by 51%, and SNR, mean capillary length, capillary density, and ICD increased by 44%, 91%, 11%, and 4.5% from single frame to 10-frame averaged, respectively. The 10-frame averaged images from the glaucomatous eyes revealed decreased density correlating to visual field defects and retinal nerve fiber layer thinning. OCTA image registration and averaging is a viable and accessible method to enhance the visualization of RPCs, with significant improvements in image quality and RPC quantitative parameters. With this technique, we will be able to non-invasively and reliably study RPC involvement in diseases such as glaucoma.

In recent years, the abuse of antibiotics has led to the pollution of soil and water environment, not only poultry husbandry and food manufacturing will be influenced to different degree, but also the human body will produce antibody. The detection of antibiotic content in production and life is imperative. In this review, we provide comprehensive information about chemical sensors and biosensors for antibiotic detection. We classify the currently reported antibiotic detection technologies into chromatography, mass spectrometry, capillary electrophoresis, optical detection, and electrochemistry, introduce some representative examples for each technology, and conclude the advantages and limitations. In particular, the optical and electrochemical methods based on nanomaterials are discussed and evaluated in detail. In addition, the latest research in the detection of antibiotics by photosensitive materials is discussed. Finally, we summarize the pros and cons of various antibiotic detection methods and present a discussion and outlook on the expansion of cross-scientific areas. The synthesis and application of optoelectronic nanomaterials and aptamer screening are discussed and prospected, and the future trends and potential impact of biosensors in antibiotic detection are outlined. Graphical abstract

To identify objective optical coherence tomography angiography (OCTA) parameters that characterize the spectrum of non-proliferative diabetic retinopathy (NPDR), especially those that distinguish moderate from severe NPDR. Sixty eyes of 60 patients with treatment-naïve NPDR (mild: 21, moderate: 21, severe: 18), 23 eyes with diabetes and no retinopathy, and 24 healthy control eyes were enrolled. OCTA slabs were segmented into superficial (SCP), middle (MCP), and deep capillary plexus (DCP) and thresholded by a new method based on DCP skeletonized vessel length. The foveal avascular zone (FAZ) area, parafoveal vessel density (VD), and adjusted flow index (AFI) from all three capillary layers and the vessel length density (VLD) of the SCP were compared between each severity group, after adjusting for age and image quality. All vessel density markers decreased with increasing severity of NPDR. SCP VD and VLD demonstrated significant differences between eyes with diabetes with no retinopathy and mild NPDR (p = 0.001 and p < 0.001, respectively), as well as between moderate vs. severe NPDR (p = 0.004 and p = 0.009, respectively). MCP VD significantly decreased between moderate and severe NPDR (p = 0.01). AFI significantly increased in the SCP and showed a decreasing trend in the MCP and DCP with increasing NPDR severity. Changes in the SCP VD, SCP VLD, and MCP VD can distinguish severe NPDR from lower-risk stages. SCP changes may be more reliable due to their lower susceptibility to noise and projection artifacts. Thresholding OCTA images based on DCP skeletonized vessel length showed less variability in moderate and severe NPDR. Additional studies are warranted to validate this new thresholding method.

The use of polycapillary optics in confocal micro‐X‐ray fluorescence analysis (CMXRF) enables the destruction‐free 3D investigation of the elemental composition of samples. The energy‐dependent transmission properties, concerning intensity and spatial beam propagation of three polycapillary half lenses, which are vital for the quantitative interpretation of such CMXRF measurements, are investigated in a monochromatic confocal laboratory setup at the Atominstitut of TU Wien, and a synchrotron setup on the BAMline beamline at the BESSY II Synchrotron, Helmholtz‐Zentrum‐Berlin. The empirically established results, concerning the intensity of the transmitted beam, are compared with theoretical values calculated with the polycap software package and a newly presented analytical model for the transmission function. The resulting form of the newly modelled energy‐dependent transmission function is shown to be in good agreement with Monte Carlo simulated results for the complete energy regime, as well as the empirically established results for the energy regime between 6 keV and 20 keV. An analysis of possible fabrication errors was conducted via pinhole scans showing only minor fabrication errors in two of the investigated polycapillary optics. The energy‐dependent focal spot size of the primary polycapillary was investigated in the laboratory via the channel‐wise evaluation of knife‐edge scans. Experimental results are compared with data given by the manufacturer as well as geometric estimations for the minimal focal spot size. Again, the resulting measurement points show a trend in agreement with geometrically estimated results and manufacturer data. A new analytical model for the transmission function of polycapillary half‐lens optics is presented. The model is compared with measurement results taken at the BAMline beamline at BESSY II and at TU Wien, as well as Monte Carlo simulations.

Chiral nano- or metamaterials and surfaces enable striking photonic properties, such as negative refractive index and superchiral light, driving promising applications in novel optical components, nanorobotics, and enhanced chiral molecular interactions with light. In characterizing chirality, although nonlinear chiroptical techniques are typically much more sensitive than their linear optical counterparts, separating true chirality from anisotropy is a major challenge. Here, we report the first observation of optical activity in second-harmonic hyper-Rayleigh scattering (HRS). We demonstrate the effect in a 3D isotropic suspension of Ag nanohelices in water. The effect is 5 orders of magnitude stronger than linear optical activity and is well pronounced above the multiphoton luminescence background. Because of its sensitivity, isotropic environment, and straightforward experimental geometry, HRS optical activity constitutes a fundamental experimental breakthrough in chiral photonics for media including nanomaterials, metamaterials, and chemical molecules.