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"Capture, 1629"
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A fleeting empire: early Stuart Britain and the merchant adventures to Canada
Before the future of North American rule was decided by the battle between British and French forces on the Plains of Abraham, Britain's emerging imperial interests were represented by ambitious merchants and privateers. A Fleeting Empire examines the lives and exploits of early European adventurers in North America, revealing the murky mix of self-interest, patriotism, and adventure that motivated them. The union of the English and Scottish crowns in 1603 gave rise to a new British seafaring community, which the early Stuart monarchy used to pursue some of the first commercial and colonial ventures in North America. Among those who sailed across the Atlantic were the Kirke brothers, who in 1629 forced Samuel de Champlain's surrender of Quebec, Sir William Alexander of Menstrie, a rising political figure and patentee of Nova Scotia, and James Stewart of Killeith, leader of a colony on Cape Breton Island. King Charles I was more concerned with brokering a peace with France than looking to the new world, so the gains of the merchant adventurers were short-lived, but their adventures provide a tantalizing glimpse of a moment of British colonial control, suggesting what might have been. Andrew Nicholls showcases the enterprises of knights and privateers alike, providing a fascinating account of early European colonies, commerce, and military force in North America. A Fleeting Empire forces us to see the early histories of Canada and the United States in a new light.
eBook
Fleeting Empire
Before the future of North American rule was decided by the battle between British and French forces on the Plains of Abraham, Britain’s emerging imperial interests were represented by ambitious merchants and privateers. A Fleeting Empire examines the lives and exploits of early European adventurers in North America, revealing the murky mix of self-interest, patriotism, and adventure that motivated them.
eBook
Spatial transcriptomic analysis of cryosectioned tissue samples with Geo-seq
Geo-seq combines laser capture microdissection and single-cell RNA-seq technology to enable transcriptome analysis of small quantities of cells from defined geographical locations.
Conventional gene expression studies analyze multiple cells simultaneously or single cells, for which the exact
in vivo
or
in situ
position is unknown. Although cellular heterogeneity can be discerned when analyzing single cells, any spatially defined attributes that underpin the heterogeneous nature of the cells cannot be identified. Here, we describe how to use geographical position sequencing (Geo-seq), a method that combines laser capture microdissection (LCM) and single-cell RNA-seq technology. The combination of these two methods enables the elucidation of cellular heterogeneity and spatial variance simultaneously. The Geo-seq protocol allows the profiling of transcriptome information from only a small number cells and retains their native spatial information. This protocol has wide potential applications to address biological and pathological questions of cellular properties such as prospective cell fates, biological function and the gene regulatory network. Geo-seq has been applied to investigate the spatial transcriptome of mouse early embryo, mouse brain, and pathological liver and sperm tissues. The entire protocol from tissue collection and microdissection to sequencing requires ∼5 d, Data analysis takes another 1 or 2 weeks, depending on the amount of data and the speed of the processor.
Journal Article
Estimating the size of populations at risk for malaria: a case study in cattle herders and agricultural workers in Northern Namibia
Cattle herders and agricultural workers have been identified has key high-risk populations for malaria in northern Namibia. Population size estimates for these groups are lacking but are important for planning, monitoring and evaluating the effectiveness of targeted strategies towards malaria elimination in the region. In this analysis, we extend population size estimation methods routinely used in HIV research, specifically social mapping and multiple source capture-recapture, to the context of malaria to estimate how many cattle herders and agricultural workers lived in two regions of northern Namibia over the course of the 2019–2020 malaria season. Both methods estimated two to three times more agricultural workers than cattle herders but size estimates based on the multiple source capture-recapture method were two to three times greater than the mapping-based, highlighting important methodological considerations to apply such methods to these highly mobile populations. In particular, we compared open versus closed populations assumptions for the capture-recapture method and assessed the impact of sensitivity analyses on the procedure to link records across multiple data sources on population size estimates. Our results are important for national control programs to target their resources and consider integrating routine population size estimation of high risk populations in their surveillance activities.
Journal Article